7,291 research outputs found

    Examples of works to practice staccato technique in clarinet instrument

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    Klarnetin staccato tekniğini güçlendirme aşamaları eser çalışmalarıyla uygulanmıştır. Staccato geçişlerini hızlandıracak ritim ve nüans çalışmalarına yer verilmiştir. Çalışmanın en önemli amacı sadece staccato çalışması değil parmak-dilin eş zamanlı uyumunun hassasiyeti üzerinde de durulmasıdır. Staccato çalışmalarını daha verimli hale getirmek için eser çalışmasının içinde etüt çalışmasına da yer verilmiştir. Çalışmaların üzerinde titizlikle durulması staccato çalışmasının ilham verici etkisi ile müzikal kimliğe yeni bir boyut kazandırmıştır. Sekiz özgün eser çalışmasının her aşaması anlatılmıştır. Her aşamanın bir sonraki performans ve tekniği güçlendirmesi esas alınmıştır. Bu çalışmada staccato tekniğinin hangi alanlarda kullanıldığı, nasıl sonuçlar elde edildiği bilgisine yer verilmiştir. Notaların parmak ve dil uyumu ile nasıl şekilleneceği ve nasıl bir çalışma disiplini içinde gerçekleşeceği planlanmıştır. Kamış-nota-diyafram-parmak-dil-nüans ve disiplin kavramlarının staccato tekniğinde ayrılmaz bir bütün olduğu saptanmıştır. Araştırmada literatür taraması yapılarak staccato ile ilgili çalışmalar taranmıştır. Tarama sonucunda klarnet tekniğin de kullanılan staccato eser çalışmasının az olduğu tespit edilmiştir. Metot taramasında da etüt çalışmasının daha çok olduğu saptanmıştır. Böylelikle klarnetin staccato tekniğini hızlandırma ve güçlendirme çalışmaları sunulmuştur. Staccato etüt çalışmaları yapılırken, araya eser çalışmasının girmesi beyni rahatlattığı ve istekliliği daha arttırdığı gözlemlenmiştir. Staccato çalışmasını yaparken doğru bir kamış seçimi üzerinde de durulmuştur. Staccato tekniğini doğru çalışmak için doğru bir kamışın dil hızını arttırdığı saptanmıştır. Doğru bir kamış seçimi kamıştan rahat ses çıkmasına bağlıdır. Kamış, dil atma gücünü vermiyorsa daha doğru bir kamış seçiminin yapılması gerekliliği vurgulanmıştır. Staccato çalışmalarında baştan sona bir eseri yorumlamak zor olabilir. Bu açıdan çalışma, verilen müzikal nüanslara uymanın, dil atış performansını rahatlattığını ortaya koymuştur. Gelecek nesillere edinilen bilgi ve birikimlerin aktarılması ve geliştirici olması teşvik edilmiştir. Çıkacak eserlerin nasıl çözüleceği, staccato tekniğinin nasıl üstesinden gelinebileceği anlatılmıştır. Staccato tekniğinin daha kısa sürede çözüme kavuşturulması amaç edinilmiştir. Parmakların yerlerini öğrettiğimiz kadar belleğimize de çalışmaların kaydedilmesi önemlidir. Gösterilen azmin ve sabrın sonucu olarak ortaya çıkan yapıt başarıyı daha da yukarı seviyelere çıkaracaktır

    Anuário científico da Escola Superior de Tecnologia da Saúde de Lisboa - 2021

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    É com grande prazer que apresentamos a mais recente edição (a 11.ª) do Anuário Científico da Escola Superior de Tecnologia da Saúde de Lisboa. Como instituição de ensino superior, temos o compromisso de promover e incentivar a pesquisa científica em todas as áreas do conhecimento que contemplam a nossa missão. Esta publicação tem como objetivo divulgar toda a produção científica desenvolvida pelos Professores, Investigadores, Estudantes e Pessoal não Docente da ESTeSL durante 2021. Este Anuário é, assim, o reflexo do trabalho árduo e dedicado da nossa comunidade, que se empenhou na produção de conteúdo científico de elevada qualidade e partilhada com a Sociedade na forma de livros, capítulos de livros, artigos publicados em revistas nacionais e internacionais, resumos de comunicações orais e pósteres, bem como resultado dos trabalhos de 1º e 2º ciclo. Com isto, o conteúdo desta publicação abrange uma ampla variedade de tópicos, desde temas mais fundamentais até estudos de aplicação prática em contextos específicos de Saúde, refletindo desta forma a pluralidade e diversidade de áreas que definem, e tornam única, a ESTeSL. Acreditamos que a investigação e pesquisa científica é um eixo fundamental para o desenvolvimento da sociedade e é por isso que incentivamos os nossos estudantes a envolverem-se em atividades de pesquisa e prática baseada na evidência desde o início dos seus estudos na ESTeSL. Esta publicação é um exemplo do sucesso desses esforços, sendo a maior de sempre, o que faz com que estejamos muito orgulhosos em partilhar os resultados e descobertas dos nossos investigadores com a comunidade científica e o público em geral. Esperamos que este Anuário inspire e motive outros estudantes, profissionais de saúde, professores e outros colaboradores a continuarem a explorar novas ideias e contribuir para o avanço da ciência e da tecnologia no corpo de conhecimento próprio das áreas que compõe a ESTeSL. Agradecemos a todos os envolvidos na produção deste anuário e desejamos uma leitura inspiradora e agradável.info:eu-repo/semantics/publishedVersio

    The Adirondack Chronology

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    The Adirondack Chronology is intended to be a useful resource for researchers and others interested in the Adirondacks and Adirondack history.https://digitalworks.union.edu/arlpublications/1000/thumbnail.jp

    Investigation of a Histidine-Based Probe for the Exploration of Proteomes

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    Leishmaniasis is a neglected tropical disease which affects 0.7-1 million people per year. Current chemotherapies for leishmaniasis are toxic with long treatment times and reports of increasing resistance, which stresses the importance of this research area. Inositol phosphorylceramide synthase is a membrane bound enzyme that has no direct human homologue, which converts ceramide to inositol phosphorylceramide through the action of a highly conserved HHD catalytic triad. An ideal method to study this enzyme further would be through activity-based protein profiling, however, there are currently no activity-based probes reported that reacts with this type of active site. Therefore, an activity-based probe was designed based on the structure of diethyl pyrocarbonate, a compound known to bind covalently to active site histidine residues. The synthesised activity-based probe was shown to inhibit Leishmania major inositol phosphorylceramide synthase in a simple assay. In addition, the probe was shown to selectively bind to the active site histidine residue in two pure enzyme models; one of which has the same catalytic triad as inositol phosphorylceramide synthase, and the other was an acid base active site histidine residue. Further, this activity-based probe was able to isolate an overexpressed enzyme in the lysate of Escherichia coli as well as bind to intrinsic proteins. Following the function validation of the activity-based probe, preliminary work was started in Leishmania to isolate proteins identify expressed enzymes

    The mechanisms of antibody generation in the llama

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    The llama is able to generate a unique class of antibody. The heavy chain immunoglobulins consist only of two heavy chain polypeptides and bind antigen specifically through single protein domains. Although the mechanisms by which such an antibody interacts with antigen has been studied at some length the manner in which the heavy chain antibody is generated within the llama is unknown. In this study a number of components of the llama immune system have been characterised. The isolation of genes encoding the variable domain of the heavy chain antibody indicates that specific genetic elements within the llama genome are responsible for the generation of the heavy chain antibody. The discovery of constant region genes that encode the heavy chain antibody provides an explanation for the absence of a major immunoglobulin domain from the final, secreted gene product. The lack of this domain within the expressed antibody is believed to be the result of a single nucleotide splice site mutation. In order to investigate the process of llama antibody generation further additional components of the llama immune system, the recombination activating genes (rag) were isolated. One such llama rag gene (rag-i) was cloned, expressed and utilised in an in vitro assay system to investigate recombination events taking place during antibody generation. This assay involved the use of specific signal sequences derived from variable domain gene sequence data and represents, to our knowledge, the first examination of non-murine RAG activity. Through the use of this system distinct differences between llama and mouse recombination signal sequences (RSSs) were uncovered. These differences, located within a specific region of the RSS known as the coding flank, may play an important role in llama antibody generation. These results have led to the proposal of a number of models for the mechanisms involved in llama antibody generation

    QAnon Propaganda on Twitter as Information Warfare: Influencers, Networks, and Narratives

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    QAnon refers to a set of far-right, conspiratorial ideologies that have risen in popularity in the U.S. since their initial promotion in 2017 on the 4chan internet message board. A central narrative element of QAnon is that a powerful group of elite, liberal members of the Democratic Party engage in morally reprehensible practices, but that former U.S. President Donald J. Trump was prosecuting them. Five studies investigated the influence and network connectivity of accounts promoting QAnon on Twitter from August, 2020 through January, 2021. Selection of Twitter accounts emphasized on-line influencers and "persons of interest" known or suspected of participation in QAnon propaganda promotion activities. Evidence of large-scale coordination among accounts promoting QAnon was observed, demonstrating rigorous, quantitative evidence of "astroturfing" in QAnon propaganda promotion on Twitter, as opposed to strictly "grassroots" activities of citizens acting independently. Further, evidence was obtained supporting that networks of extreme far-right adherents engaged in organized QAnon propaganda promotion, as revealed by network overlap among accounts promoting far-right extremist (e.g., anti-Semitic) content and insurrectionist themes; New Age, occult, and "esoteric" themes; and internet puzzle games like Cicada 3301 and other "alternate reality games." Based on well-grounded theories and findings from the social sciences, it is argued that QAnon propaganda on Twitter in the months circa the 2020 U.S. Presidential election likely reflected joint participation of multiple actors, including nation-states like Russia, in innovative misuse of social media toward undermining democratic processes by promoting "magical" thinking, ostracism of Democrats and liberals, and salience of White extinction narratives common among otherwise ideologically diverse groups on the extreme far-right.Comment: 60 pages, 14 figure

    Modélisation et comparaison de la structure de gènes

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    La bio-informatique est un domaine de recherche multi-disciplinaire, à la croisée de différents domaines : biologie, médecine, mathématiques, statistiques, chimie, physique et informatique. Elle a pour but de concevoir et d’appliquer des modèles et outils statistiques et computationnels visant l’avancement des connaissances en biologie et dans les sciences connexes. Dans ce contexte, la compréhension du fonctionnement et de l’évolution des gènes fait l’objet de nombreuses études en bio-informatique. Ces études sont majoritairement fondées sur la comparaison des gènes et en particulier sur l’alignement de séquences génomiques. Cependant, dans leurs calculs d’alignement de séquences génomiques, les méthodes existantes se basent uniquement sur la similarité des séquences et ne tiennent pas compte de la structure des gènes. L’alignement prenant en compte la structure des séquences offre l’opportunité d’en améliorer la précision ainsi que les résultats des méthodes développées à partir de ces alignements. C’est dans cette hypothèse que s’inscrit l’objectif de cette thèse de doctorat : proposer des modèles tenant compte de la structure des gènes lors de l’alignement des séquences de familles de gènes. Ainsi, par cette thèse, nous avons contribué à accroître les connaissances scientifiques en développant des modèles d’alignement de séquences biologiques intégrant des informations sur la structure de codage et d’épissage des séquences. Nous avons proposé un algorithme et une nouvelle fonction du score pour l’alignement de séquences codantes d’ADN (CDS) en tenant compte de la longueur des décalages du cadre de traduction. Nous avons aussi proposé un algorithme pour aligner des paires de séquences d’une famille de gènes en considérant leurs structures d’épissage. Nous avons également développé un algorithme pour assembler des alignements épissés par paire en alignements multiples de séquences. Enfin, nous avons développé un outil pour la visualisation d’alignements épissés multiples de famille de gènes. Dans cette thèse, nous avons souligné l’importance et démontré l’utilité de tenir compte de la structure des séquences en entrée lors du calcul de leur alignement

    Applications of nanopore DNA sequencing for improved genome assembly

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    An organism\u27s genome is the ultimate determinant of its functional potential. Understanding genomes is therefore essential to understand function, and a foundational knowledge of a genome is required transfer functions to and from microorganisms of interest. Sequencing DNA using nanopores is a recent advance that resolves limitations of previous technologies, enabling an improved understanding of genomes. For this thesis, I improved our understanding of microbial genomes by developing novel approaches to analyze long read sequencing data, setting the foundation for future synthetic biology work. Long sequencing reads have enabled routine assembly of complete bacterial genomes by directly sequencing DNA extracted from bacterial communities. I showed that visualizing sequencing coverage after filtering read alignments using a 95\% query coverage cutoff (i.e., the entire read aligns to the genome) enabled the detection of mis-assemblies. I also showed it can be applied to detect recoverable alternate haplotypes containing important functional elements. Furthermore, I used this approach to demonstrate that a circular genome for a novel species of Saccharibacteria, enriched from a heavy-metal polluted Northern Albertan tailings pond, contains a recently acquired genomic island. I also determined this genomic island encodes heavy metal-resistance genes, suggesting that horizontal gene transfer may be possible under selective pressure in Saccharibacteria. Another track of my thesis focused on applying nanopore sequencing on a marine diatom, Phaeodactylum tricornutum, which has significant interest for synthetic biology applications like producing low-cost glycosylated proteins. This species does not have a complete genome assembly, despite a draft sequence being available since 2008. To determine the full structure of the genome, I used ultra-long sequencing reads to build a telomere-to-telomere genome assembly. I also developed a novel, assembly-free approach to determine the number of chromosomes from eukaryotes directly from nanopore sequencing reads as an orthogonal method to validate the assembly, which I term long-read karyocounting. These studies provide complete genome assemblies for both novel bacterial species and a marine diatom who\u27s genome structure had yet to be resolved. These approaches also demonstrate that there is more information encoded in long read sequencing data than just the sum of assembled sequence
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