13,712 research outputs found

    Holistic analysis of lysine acetylation in aquaculture pathogenic bacteria Vibrio alginolyticus under bile salt stress

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    Lysine acetylation modification is a dynamic and reversible post-translational modification, which plays an important role in the metabolism and pathogenicity of pathogenic bacteria. Vibrio alginolyticus is a common pathogenic bacterium in aquaculture, and bile salt can trigger the expression of bacterial virulence. However, little is known about the function of lysine acetylation in V. alginolyticus under bile salt stress. In this study, 1,315 acetylated peptides on 689 proteins were identified in V. alginolyticus under bile salt stress by acetyl-lysine antibody enrichment and high-resolution mass spectrometry. Bioinformatics analysis found that the peptides motif ****A*Kac**** and *******Kac****A* were highly conserved, and protein lysine acetylation was involved in regulating various cellular biological processes and maintaining the normal life activities of bacteria, such as ribosome, aminoacyl-tRNA biosynthesis, fatty acid metabolism, two-component system, and bacterial secretion system. Further, 22 acetylated proteins were also found to be related to the virulence of V. alginolyticus under bile salt stress through secretion system, chemotaxis and motility, and adherence. Finally, comparing un-treated and treated with bile salt stress lysine acetylated proteins, it was found that there were 240 overlapping proteins, and found amino sugar and nucleotide sugar metabolism, beta-Lactam resistance, fatty acid degradation, carbon metabolism, and microbial metabolism in diverse environments pathways were significantly enriched in bile salt stress alone. In conclusion, this study is a holistic analysis of lysine acetylation in V. alginolyticus under bile salt stress, especially many virulence factors have also acetylated

    Drift of ablated material after pellet injection in a tokamak

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    Pellet injection is used for fuelling and controlling discharges in tokamaks, and it is foreseen in ITER. During pellet injection, a movement of the ablated material towards the low-field side (or outward major radius direction) occurs because of the inhomogeneity of the magnetic field. Due to the complexity of the theoretical models, computer codes developed to simulate the cross-field drift are computationally expensive. Here, we present a one-dimensional semi-analytical model for the radial displacement of ablated material after pellet injection, taking into account both the Alfv\'en and ohmic currents which short-circuit the charge separation creating the drift. The model is suitable for rapid calculation of the radial drift displacement, and can be useful for e.g. modelling of disruption mitigation via pellet injection.Comment: 22 pages, 4 figures. Submitted to Journal of Plasma Physic

    Elite perceptions of the Victorian and Edwardian past in inter-war England

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    It is often argued by historians that members of the cultivated Elite after 1918 rejected the pre-war past. or at least subjected it to severe denigration. This thesis sets out to challenge such a view. Above all, it argues that inter-war critics of the Victorian and Edwardian past were unable to reject it even if that was what they felt inclined to do. This was because they were tied to those periods by the affective links of memory, family, and the continually unfolding consequences of the past in the present. Even the severest critics of the pre-war world, such as Lytton Strachey, were less frequently dismissive of history than ambivalent towards it. This ambivalence, it is argued, helped to keep the past alive and often to humanise it. The thesis also explores more positive estimation of Victorian and Edwardian history between the wars. It examines nostalgia for the past, as well as instances of continuity of practice and attitude. It explores the way in which inter-war society drew upon aspects of Victorian and Edwardian history both as illuminating parallels to contemporary affairs and to understand directly why the present was shaped as it was. Again, this testifies to the enduring power of the past after 1918. There are three parts to this thesis. Part One outlines the cultural context in which writers contemplated the Victorian and Edwardian past. Part Two explores some of the ways in which history was written about and used by inter-war society. Part Three examines the ways in which biographical depictions of eminent Victorians after 1918 encouraged emotional negotiation with the pas

    Victims' Access to Justice in Trinidad and Tobago: An exploratory study of experiences and challenges of accessing criminal justice in a post-colonial society

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    This thesis investigates victims' access to justice in Trinidad and Tobago, using their own narratives. It seeks to capture how their experiences affected their identities as victims and citizens, alongside their perceptions of legitimacy regarding the criminal justice system. While there have been some reforms in the administration of criminal justice in Trinidad and Tobago, such reforms have not focused on victims' accessibility to the justice system. Using grounded theory methodology, qualitative data was collected through 31 in-depth interviews with victims and victim advocates. The analysis found that victims experienced interpersonal, structural, and systemic barriers at varying levels throughout the criminal justice system, which manifested as institutionalized secondary victimization, silencing and inequality. This thesis argues that such experiences not only served to appropriate conflict but demonstrates that access is often given in a very narrow sense. Furthermore, it shows a failure to encompass access to justice as appropriated conflicts are left to stagnate in the system as there is often very little resolution. Adopting a postcolonial lens to analyse victims' experiences, the analysis identified othering practices that served to institutionalize the vulnerability and powerlessness associated with victim identities. Here, it is argued that these othering practices also affected the rights consciousness of victims, delegitimating their identities as citizens. Moreover, as a result of their experiences, victims had mixed perceptions of the justice system. It is argued that while the system is a legitimate authority victims' endorsement of the system is questionable, therefore victims' experiences suggest that there is a reinforcement of the system's legal hegemony. The findings suggest that within the legal system of Trinidad and Tobago, legacies of colonialism shape the postcolonial present as the psychology and inequalities of the past are present in the interactions and processes of justice. These findings are relevant for policymakers in Trinidad and Tobago and other regions. From this study it is recognized that, to improve access to justice for victims, there needs to be a move towards victim empowerment that promotes resilience and enhances social capital. Going forward it is noted that there is a need for further research

    Association of the onset of self-feeding with subsequent Developmental Coordination Disorder: A prospective cohort study in China

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    Background: Successful self-feeding reflects the readiness of early motor development and environmental impacts, and the onset of self-feeding as a developmental milestone might be a predictor of subsequent motor development in children. In this study, we explored the association between the onset of self-feeding and childhood risk of Developmental Coordination Disorder in children from one-child and two-child families. Methods: We conducted a data-linkage prospective cohort study from 38 kindergartens in 6 cities in China. A total of 11,727 preschoolers were included in the final analysis and were assessed with the Movement Assessment Battery for Children-second edition (MABC-2) Test. The information on early self-feeding onset was obtained from parents. The mixed and multi-level logistic models utilizing a random intercept were used to investigate the associations between the onset time of self-feeding and subsequent motor performance. Results: The results showed that, compared with those beginning self-feeding at or younger than 12 months of age, children starting self-feeding at 13-24 months, 25-36 months, and later than 36 months, showed a decrease in their total MABC-2 scores of 2.181, 3.026 and 3.874, respectively; and had an increased risk of suspected DCD by 36.0%, 101.6%, 102.6% respectively; they also had 30.2%, 46.6%, 71.2% increased prevalence of at risk of suspected DCD, when adjusting for both child and family characteristics (each p<0.05). Significant associations were observed in fine motor, gross motor, and balance subtests (each p<0.05) in groups with a delayed onset of self-feeding. However, the strength of the associations was mitigated in the fine motor and balance subtests in children with a sibling. Conclusion: The delayed onset time of self-feeding acts as an early behavioural marker for later childhood motor impairment. Moreover, children with a sibling may benefit from additional interaction and their motor developmental pattern may be affected by the presence of a sibling.

    Queer spies in British Cold War culture: literature, film, theatre and television

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    This PhD thesis investigates how male homosexuality has been represented in British spy fiction from the 1950s to the 2010s in multiple media: literature, film, television and theatre. Due mainly to the betrayal of the Cambridge Spy ring around the middle of the century, British culture has associated spies with homosexuality, while the wider Anglophone world was in the grip of a homophobic atmosphere created by McCarthy's Red Scare. My thesis explores how this history is reflected in the spy genre from the Cold War to the present, in which male homosexuality and secret agency intersect as “queer”, in so far as they were both considered to be discreet and criminal, existing outside of the heteronormative order. By following multiple texts across media and time, I discuss how some writers, television and film directors and actors update queer identity in spy fiction, creating a shifting image of queer spies through decades. I refer to the findings of adaptation studies and queer studies, along with numerous studies on spy fiction. I conclude that the interrelation of different media has contributed to the re-drawing of queer identity in spy fiction. These developments have enabled the spies' queer identity to transcend its pejorative history in British culture, towards its more flexible and pliant sense which is designated by the term's modern usage. I also discuss that spies’ homosexuality has been represented as a fleeting ghost in most of the texts examined, hovering on the margins of pages and screen. Although homosexuality is not “the love that dare not speak its name” anymore, clandestine queer spies have been preserved as spectral others in the genre for many years. Spy fiction is a cultural repository retaining the memory of violence inflicted against those who have been called “queer” in twentieth century Britain, and the spectral nature of queer spies narrates this history reaching back to the Oscar Wilde trial in 1895, from which point British queer identity as we know now developed. This thesis benefits the study of spy fiction by filling a gap in the investigation of homosexual representation. It also contributes to the field of gender studies of literature, film, television, and theatre by illustrating queer history in a genre which has not received a great deal of focus on its representation of homosexuality. Spy fiction occupies a central position in British popular culture, and by exploring this genre in terms of homosexuality, this research will identify the role which same-sex desire has historically played in the British cultural imagination

    Early Neanderthal social and behavioural complexity during the Purfleet Interglacial: handaxes in the latest Lower Palaeolithic.

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    Only a handful of ‘flagship’ sites from the Purfleet Interglacial (Marine Isotope Stage 9, c. 350-290,000 years ago) have been properly examined, but the archaeological succession at the proposed type-site at Purfleet suggests a period of complexity and transition, with three techno-cultural groups represented in Britain. The first was a simple toolkit lacking handaxes (the Clactonian), and the last a more sophisticated technology presaging the coming Middle Palaeolithic (simple prepared core or proto-Levallois technology). Sandwiched between were Acheulean groups, whose handaxes comprise the great majority of the extant archaeological record of the period – these are the focus of this study. It has previously been suggested that some features of the Acheulean in the Purfleet Interglacial were chronologically restricted, particularly the co-occurrence of ficrons and cleavers. These distinctive forms may have exceeded pure functionality and were perhaps imbued with a deeper social and cultural meaning. This study supports both the previously suggested preference for narrow, pointed morphologies, and the chronologically restricted pairing of ficrons and cleavers. By drawing on a wide spatial and temporal range of sites these patterns could be identified beyond the handful of ‘flagship’ sites previously studied. Hypertrophic ‘giants’ have now also been identified as a chronologically restricted form. Greater metrical variability was found than had been anticipated, leading to the creation of two new sub-groups (IA and IB) which are tentatively suggested to represent spatial and perhaps temporal patterning. The picture in the far west of Britain remains unclear, but the possibility of different Acheulean groups operating in the Solent area, and a late survival of the Acheulean, are both suggested. Handaxes with backing and macroscopic asymmetry may represent prehensile or ergonomic considerations not commonly found on handaxes from earlier interglacial periods. It is argued that these forms anticipate similar developments in the Late Middle Palaeolithic in an example of convergent evolution

    “Things I can remember about my life”: Autobiography and fatherhood in Victorian Britain

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    It is now nearly forty years since John Burnett, David Vincent, and David Mayall compiled their invaluable and much-used three-volume finding aid, The Autobiography of the Working Class: An Annotated, Critical Bibliography (1984-1989), and established working-class autobiography as an important documentary source for exploring the lives of the working poor. Life writing now forms the basis of historical research into areas such as the emotions and domestic life that had hardly been imagined at the time that the annotated bibliography was produced. Yet as research into working-class autobiography has extended into new domains of enquiry, there has been less innovation in methodology. Historians typically use autobiographical material to pursue deep-reading strategies and unpack the meaning, experience, and identity of individual writers rather than generalize about working-class life more broadly. In this article I offer an alternative strategy: to take the autobiographical corpus and read it at scale in order to better understand fatherhood in Victorian Britain. Through a combination of intensive and extensive reading, I demonstrate that many working-class men failed to live up to expectations as breadwinners, and I explore the ramifications of that failure for the women and children with whom they lived

    Cooking the wild: the role of the Lundayeh of the Ulu Padas, (Sabah, Malaysia) in managing forest foods and shaping the landscape

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    This thesis provides an account of the Lundayeh subsistence system as found in the villages of Long Pasia and Long Mio, situated in the Ulu Padas, Sabah. The research focuses on Lundayeh food and diet, describing the diversity of resources used and the importance of forest foods. Comparison with studies from elsewhere in Borneo suggests that there are many similarities between Lundayeh practices and those of other highland peoples. These data are used to critically examine the concepts of 'wild' and 'wilderness', considering whether these concepts are meaningful, either analytically or for the Lundayeh. Investigation of the way in which the Lundayeh manipulate and manage their resources suggests that they have had a profound influence on their environment. Consequently, the Ulu Padas cannot be described as a wilderness, nor its resources as wild. The extent to which the Lundayeh themselves construct the categories of 'wild' and 'cultivated' foods is investigated through examining how these resources are owned, and their different roles in the diet. These data suggest that the Lundayeh recognise that there is no simple dichotomy of 'wild' and 'cultivated', but rather, that there is a gradation between these two categories. There is also evidence to suggest that the Lundayeh do not consider any resources as wild, in the sense of being uninfluenced by people. The environmental perceptions of the Lundayeh are also investigated, and how these have been shaped by their particular way of life, history, beliefs and knowledge systems. It is apparent that for the Lundayeh, the Ulu Padas is a cultural landscape. However, this is changing, as a result of recent social and environmental changes. This thesis concludes by examining the impact of changing perceptions on how the Lundayeh are managing their environment, and on their attitudes towards conservation

    Microservice Architecture Reconstruction and Visualization Techniques: A Review

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    Microservice system solutions are driving digital transformation; however, fundamental tools and system perspectives are missing to better observe, understand, and manage these systems, their properties, and their dependencies. Microservices architecture leads towards decentralization, which implies many advantages to system operation; it, however, brings challenges to their development. Microservice systems often lack a system-centric perspective that would help engineers better cope with system evolution and quality assessment. In this work, we explored microservice-specific architecture reconstruction based on static analysis. Such reconstruction typically results in system models to visualize selected system-centric perspectives. Conventional models involve 2D methods; however, these methods are limited in utility when services proliferate. We considered various architectural perspectives relevant to microservices and assessed the relevancy of the traditional method, comparing it to alternative data visualization using 3D space. As a representative of the 3D method, we considered a 3D graph model presented in augmented reality. To begin testing the feasibility of deriving such perspectives from microservice systems, we developed and implemented prototype tools for software architecture reconstruction and visualization of compared perspectives. Using these prototypes, we performed a small user study with software practitioners to highlight the potentials and limitations of these innovative visualizations used for common practitioner reasoning and tasks
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