17 research outputs found
21st Rocky Mountain Conference on Analytical Chemistry
Abstracts and meeting program from the 21st annual meeting of the Rocky Mountain Conference on Analytical Chemistry, co-sponsored by the Rocky Mountain Section of the Society for Applied Spectroscopy and the Rocky Mountain Chromatography Discussion Group. Held in Denver, Colorado, July 30 - August 1, 1979
COMPUTER APPLICATIONS IN QUANTITATIVE EPR SPECTROSCOPY OF METALLOPROTEINS (FERRITIN, APOFERRITIN, IRON)
This dissertation examines the use of computers in quantitative EPR spectroscopy. The computers used ranged from hand held calculators to large main frame systems. Applications discussed are protein assay calculations, an EPR minicomputer interface and software system and the modification of an existing EPR simulation program to include corrections for strains in the g and A tensors. The modification permits more accurate linewidth simulation for lines with large M(,I) values. The computer interface and software allows for the collection of EPR spectra, which can then be stored, scaled, added, subtracted (for comparison) and double integrated. The program enhances weak signals by signal averaging. Double integration was used to assist in the study of early iron binding in horse spleen apoferritin. Iron(II) was added to apoferritin followed by oxidation by a variety of methods. In all cases an iron(III) EPR signal was observed at g\u27 = 4.3 which was attributed to mononuclear Fe(III) bound to the protein; this signal increased until 0.5 equivalent per subunit of added iron. In another experiment increasing amounts of Tb(III) were added to apoferritin solutions. Subsequent addition of 0.5 equivalent of iron(II) per subunit resulted in an Fe(III) signal that decreased as a function of added Tb(III). It was also found that ultracentrifugation of commercial ferritin yields a light, low iron content, fraction which showed a majority of the iron signal intensity relative to the heavy, iron rich, fraction. These results suggest that iron core starts to form at an initial binding site that lies between two adjacent subunits resulting in a 0.5 equivalent of binding site per subunit and that this site also serves as the nucleus of core formation within the ferritin molecule. As the core grows beyond 0.5 equivalents per subunit more of the mononuclear sites are converted into growing core. At 0.5 equivalents per subunit double integration shows that only 20% of the added iron is EPR active suggesting a majority of the added iron is present as polymeric iron (core) species
23rd Rocky Mountain Conference
Abstracts and meeting program from the 23rd annual meeting of the Rocky Mountain Conference, co-sponsored by the Rocky Mountain Section of the Society for Applied Spectroscopy and the Rocky Mountain Chromatography Discussion Group. Held in Denver, Colorado, August 2-6, 1981
Research Reports: 1983 NASA/ASEE Summer Faculty Fellowship Program
Thirty-five technical reports contain results of investigations in information and electronic systems; materials and processing; systems dynamics; structures and propulsion; and space sciences. Ecology at KSC, satellite de-spin, and the X-ray source monitor were also studied
Recommended from our members
1995 BRAC Commission
Naval Research Lab, Washington Site: Data Call. Tabular data, memos, documents. Box 178, L-110
Reports to the President
A compilation of annual reports for the 1985-1986 academic year, including a report from the President of the Massachusetts Institute of Technology, as well as reports from the academic and administrative units of the Institute. The reports outline the year's goals, accomplishments, honors and awards, and future plans
Measurement of Elements in the Stratosphere
Balloon-borne winch system; stratospheric free radicals; stratospheric sounding; copper vapor lasers; ozone measurement; NO2 analysis; chlorine chemistry; trace elements; and ClO observations are discussed
Energy: A continuing bibliography with indexes
This bibliography lists 1920 reports, articles, and other documents introduced into the NASA Scientific and Technical Information System from July 1, 1980 through September 30, 1980