12 research outputs found
M6AMRFS: Robust Prediction of N6-Methyladenosine Sites With Sequence-Based Features in Multiple Species
As one of the well-studied RNA methylation modifications, N6-methyladenosine (m6A) plays important roles in various biological progresses, such as RNA splicing and degradation, etc. Identification of m6A sites is fundamentally important for better understanding of their functional mechanisms. Recently, machine learning based prediction methods have emerged as an effective approach for fast and accurate identification of m6A sites. In this paper, we proposed “M6AMRFS”, a new machine learning based predictor for the identification of m6A sites. In this predictor, we exploited a new feature representation algorithm to encode RNA sequences with two feature descriptors (dinucleotide binary encoding and Local position-specific dinucleotide frequency), and used the F-score algorithm combined with SFS (Sequential Forward Search) to enhance the feature representation ability. To predict m6A sites, we employed the eXtreme Gradient Boosting (XGBoost) algorithm to build a predictive model. Benchmarking results showed that the proposed predictor is competitive with the state-of-the art predictors. Importantly, robust predictions for multiple species by our predictor demonstrate that our predictive models have strong generalization ability. To the best of our knowledge, M6AMRFS is the first tool that can be used for the identification of m6A sites in multiple species. To facilitate the use of our predictor, we have established a user-friendly webserver with the implementation of M6AMRFS, which is currently available in http://server.malab.cn/M6AMRFS/. We anticipate that it will be a useful tool for the relevant research of m6A sites
Global analysis of N6-methyladenosine functions and its disease association using deep learning and network-based methods
<div><p>N6-methyladenosine (m<sup>6</sup>A) is the most abundant methylation, existing in >25% of human mRNAs. Exciting recent discoveries indicate the close involvement of m<sup>6</sup>A in regulating many different aspects of mRNA metabolism and diseases like cancer. However, our current knowledge about how m<sup>6</sup>A levels are controlled and whether and how regulation of m<sup>6</sup>A levels of a specific gene can play a role in cancer and other diseases is mostly elusive. We propose in this paper a computational scheme for predicting m<sup>6</sup>A-regulated genes and m<sup>6</sup>A-associated disease, which includes Deep-m<sup>6</sup>A, the first model for detecting condition-specific m<sup>6</sup>A sites from MeRIP-Seq data with a single base resolution using deep learning and Hot-m<sup>6</sup>A, a new network-based pipeline that prioritizes functional significant m<sup>6</sup>A genes and its associated diseases using the Protein-Protein Interaction (PPI) and gene-disease heterogeneous networks. We applied Deep-m<sup>6</sup>A and this pipeline to 75 MeRIP-seq human samples, which produced a compact set of 709 functionally significant m<sup>6</sup>A-regulated genes and nine functionally enriched subnetworks. The functional enrichment analysis of these genes and networks reveal that m<sup>6</sup>A targets key genes of many critical biological processes including transcription, cell organization and transport, and cell proliferation and cancer-related pathways such as Wnt pathway. The m<sup>6</sup>A-associated disease analysis prioritized five significantly associated diseases including leukemia and renal cell carcinoma. These results demonstrate the power of our proposed computational scheme and provide new leads for understanding m<sup>6</sup>A regulatory functions and its roles in diseases.</p></div
NCNet: Deep Learning Network Models for Predicting Function of Non-coding DNA
The human genome consists of 98.5% non-coding DNA sequences, and most of them have no known function. However, a majority of disease-associated variants lie in these regions. Therefore, it is critical to predict the function of non-coding DNA. Hence, we propose the NCNet, which integrates deep residual learning and sequence-to-sequence learning networks, to predict the transcription factor (TF) binding sites, which can then be used to predict non-coding functions. In NCNet, deep residual learning networks are used to enhance the identification rate of regulatory patterns of motifs, so that the sequence-to-sequence learning network may make the most out of the sequential dependency between the patterns. With the identity shortcut technique and deep architectures of the networks, NCNet achieves significant improvement compared to the original hybrid model in identifying regulatory markers
Computational Methods for the Analysis of Genomic Data and Biological Processes
In recent decades, new technologies have made remarkable progress in helping to understand biological systems. Rapid advances in genomic profiling techniques such as microarrays or high-performance sequencing have brought new opportunities and challenges in the fields of computational biology and bioinformatics. Such genetic sequencing techniques allow large amounts of data to be produced, whose analysis and cross-integration could provide a complete view of organisms. As a result, it is necessary to develop new techniques and algorithms that carry out an analysis of these data with reliability and efficiency. This Special Issue collected the latest advances in the field of computational methods for the analysis of gene expression data, and, in particular, the modeling of biological processes. Here we present eleven works selected to be published in this Special Issue due to their interest, quality, and originality
Identification of Phage Viral Proteins With Hybrid Sequence Features
The uniqueness of bacteriophages plays an important role in bioinformatics research. In real applications, the function of the bacteriophage virion proteins is the main area of interest. Therefore, it is very important to classify bacteriophage virion proteins and non-phage virion proteins accurately. Extracting comprehensive and effective sequence features from proteins plays a vital role in protein classification. In order to more fully represent protein information, this paper is more comprehensive and effective by combining the features extracted by the feature information representation algorithm based on sequence information (CCPA) and the feature representation algorithm based on sequence and structure information. After extracting features, the Max-Relevance-Max-Distance (MRMD) algorithm is used to select the optimal feature set with the strongest correlation between class labels and low redundancy between features. Given the randomness of the samples selected by the random forest classification algorithm and the randomness features for producing each node variable, a random forest method is employed to perform 10-fold cross-validation on the bacteriophage protein classification. The accuracy of this model is as high as 93.5% in the classification of phage proteins in this study. This study also found that, among the eight physicochemical properties considered, the charge property has the greatest impact on the classification of bacteriophage proteins These results indicate that the model discussed in this paper is an important tool in bacteriophage protein research
Identifying Plant Pentatricopeptide Repeat Coding Gene/Protein Using Mixed Feature Extraction Methods
Motivation: Pentatricopeptide repeat (PPR) is a triangular pentapeptide repeat domain that plays a vital role in plant growth. In this study, we seek to identify PPR coding genes and proteins using a mixture of feature extraction methods. We use four single feature extraction methods focusing on the sequence, physical, and chemical properties as well as the amino acid composition, and mix the features. The Max-Relevant-Max-Distance (MRMD) technique is applied to reduce the feature dimension. Classification uses the random forest, J48, and naïve Bayes with 10-fold cross-validation.Results: Combining two of the feature extraction methods with the random forest classifier produces the highest area under the curve of 0.9848. Using MRMD to reduce the dimension improves this metric for J48 and naïve Bayes, but has little effect on the random forest results.Availability and Implementation: The webserver is available at: http://server.malab.cn/MixedPPR/index.jsp
k-Skip-n-Gram-RF: A Random Forest Based Method for Alzheimer's Disease Protein Identification
In this paper, a computational method based on machine learning technique for identifying Alzheimer's disease genes is proposed. Compared with most existing machine learning based methods, existing methods predict Alzheimer's disease genes by using structural magnetic resonance imaging (MRI) technique. Most methods have attained acceptable results, but the cost is expensive and time consuming. Thus, we proposed a computational method for identifying Alzheimer disease genes by use of the sequence information of proteins, and classify the feature vectors by random forest. In the proposed method, the gene protein information is extracted by adaptive k-skip-n-gram features. The proposed method can attain the accuracy to 85.5% on the selected UniProt dataset, which has been demonstrated by the experimental results
Recent Advances on the Machine Learning Methods in Identifying DNA Replication Origins in Eukaryotic Genomics
The initiate site of DNA replication is called origins of replication (ORI) which is regulated by a set of regulatory proteins and plays important roles in the basic biochemical process during cell growth and division in all living organisms. Therefore, the study of ORIs is essential for understanding the cell-division cycle and gene expression regulation so that scholars can develop a new strategy against genetic diseases by using the knowledge of DNA replication. Thus, the accurate identification of ORIs will provide key clues for DNA replication research and clinical medicine. Although, the conventional experiments could provide accurate results, they are time-consuming and cost ineffective. On the contrary, bioinformatics-based methods can overcome these shortcomings. Especially, with the emergence of DNA sequences in the post-genomic era, it is highly expected to develop high throughput tools to identify ORIs based on sequence information. In this review, we will summarize the current progress in computational prediction of eukaryotic ORIs including the collection of benchmark dataset, the application of machine learning-based techniques, the results obtained by these methods, and the construction of web servers. Finally, we gave the future perspectives on ORIs prediction. The review provided readers with a whole background of ORIs prediction based on machine learning methods, which will be helpful for researchers to study DNA replication in-depth and drug therapy of genetic defect
Unveiling the frontiers of deep learning: innovations shaping diverse domains
Deep learning (DL) enables the development of computer models that are
capable of learning, visualizing, optimizing, refining, and predicting data. In
recent years, DL has been applied in a range of fields, including audio-visual
data processing, agriculture, transportation prediction, natural language,
biomedicine, disaster management, bioinformatics, drug design, genomics, face
recognition, and ecology. To explore the current state of deep learning, it is
necessary to investigate the latest developments and applications of deep
learning in these disciplines. However, the literature is lacking in exploring
the applications of deep learning in all potential sectors. This paper thus
extensively investigates the potential applications of deep learning across all
major fields of study as well as the associated benefits and challenges. As
evidenced in the literature, DL exhibits accuracy in prediction and analysis,
makes it a powerful computational tool, and has the ability to articulate
itself and optimize, making it effective in processing data with no prior
training. Given its independence from training data, deep learning necessitates
massive amounts of data for effective analysis and processing, much like data
volume. To handle the challenge of compiling huge amounts of medical,
scientific, healthcare, and environmental data for use in deep learning, gated
architectures like LSTMs and GRUs can be utilized. For multimodal learning,
shared neurons in the neural network for all activities and specialized neurons
for particular tasks are necessary.Comment: 64 pages, 3 figures, 3 table