4,693 research outputs found
Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering
Background: The production of bioethanol from lignocellulose hydrolysates requires a robust, D-xylose-fermenting and inhibitor-tolerant microorganism as catalyst. The purpose of the present work was to develop such a strain from a prime industrial yeast strain, Ethanol Red, used for bioethanol production.
Results: An expression cassette containing 13 genes including Clostridium phytofermentans XylA, encoding D-xylose isomerase (XI), and enzymes of the pentose phosphate pathway was inserted in two copies in the genome of Ethanol Red. Subsequent EMS mutagenesis, genome shuffling and selection in D-xylose-enriched lignocellulose hydrolysate, followed by multiple rounds of evolutionary engineering in complex medium with D-xylose, gradually established efficient D-xylose fermentation. The best-performing strain, GS1.11-26, showed a maximum specific D-xylose consumption rate of 1.1 g/g DW/h in synthetic medium, with complete attenuation of 35 g/L D-xylose in about 17 h. In separate hydrolysis and fermentation of lignocellulose hydrolysates of Arundo donax (giant reed), spruce and a wheat straw/hay mixture, the maximum specific D-xylose consumption rate was 0.36, 0.23 and 1.1 g/g DW inoculum/h, and the final ethanol titer was 4.2, 3.9 and 5.8% (v/v), respectively. In simultaneous saccharification and fermentation of Arundo hydrolysate, GS1.11-26 produced 32% more ethanol than the parent strain Ethanol Red, due to efficient D-xylose utilization. The high D-xylose fermentation capacity was stable after extended growth in glucose. Cell extracts of strain GS1.11-26 displayed 17-fold higher XI activity compared to the parent strain, but overexpression of XI alone was not enough to establish D-xylose fermentation. The high D-xylose consumption rate was due to synergistic interaction between the high XI activity and one or more mutations in the genome. The GS1.11-26 had a partial respiratory defect causing a reduced aerobic growth rate.
Conclusions: An industrial yeast strain for bioethanol production with lignocellulose hydrolysates has been developed in the genetic background of a strain widely used for commercial bioethanol production. The strain uses glucose and D-xylose with high consumption rates and partial cofermentation in various lignocellulose hydrolysates with very high ethanol yield. The GS1.11-26 strain shows highly promising potential for further development of an all-round robust yeast strain for efficient fermentation of various lignocellulose hydrolysates
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The Alliance of Genome Resources: Building a Modern Data Ecosystem for Model Organism Databases.
Model organisms are essential experimental platforms for discovering gene functions, defining protein and genetic networks, uncovering functional consequences of human genome variation, and for modeling human disease. For decades, researchers who use model organisms have relied on Model Organism Databases (MODs) and the Gene Ontology Consortium (GOC) for expertly curated annotations, and for access to integrated genomic and biological information obtained from the scientific literature and public data archives. Through the development and enforcement of data and semantic standards, these genome resources provide rapid access to the collected knowledge of model organisms in human readable and computation-ready formats that would otherwise require countless hours for individual researchers to assemble on their own. Since their inception, the MODs for the predominant biomedical model organisms [Mus sp (laboratory mouse), Saccharomyces cerevisiae, Drosophila melanogaster, Caenorhabditis elegans, Danio rerio, and Rattus norvegicus] along with the GOC have operated as a network of independent, highly collaborative genome resources. In 2016, these six MODs and the GOC joined forces as the Alliance of Genome Resources (the Alliance). By implementing shared programmatic access methods and data-specific web pages with a unified "look and feel," the Alliance is tackling barriers that have limited the ability of researchers to easily compare common data types and annotations across model organisms. To adapt to the rapidly changing landscape for evaluating and funding core data resources, the Alliance is building a modern, extensible, and operationally efficient "knowledge commons" for model organisms using shared, modular infrastructure
Tv-RIO1 – an atypical protein kinase from the parasitic nematode Trichostrongylus vitrinus
Background: Protein kinases are key enzymes that regulate a wide range of cellular processes, including cell-cycle progression, transcription, DNA replication and metabolic functions. These enzymes catalyse the transfer of phosphates to serine, threonine and tyrosine residues, thus playing functional roles in reversible protein phosphorylation. There are two main groups, namely eukaryotic protein kinases (ePKs) and atypical protein kinases (aPKs); RIO kinases belong to the latter group. While there is some information about RIO kinases and their roles in animals, nothing is known about them in parasites. This is the first study to characterise a RIO1 kinase from any parasite.
Results: A full-length cDNA (Tv-rio-1) encoding a RIO1 protein kinase (Tv-RIO1) was isolated from the economically important parasitic nematode Trichostrongylus vitrinus (Order Strongylida). The uninterrupted open reading frame (ORF) of 1476 nucleotides encoded a protein of 491 amino acids, containing the characteristic RIO1 motif LVHADLSEYNTL. Tv-rio-1 was transcribed at the highest level in the third-stage larva (L3), and a higher level in adult females than in males. Comparison with homologues from other organisms showed that protein Tv-RIO1 had significant homology to related proteins from a range of metazoans and plants. Amino acid sequence identity was most pronounced in the ATP-binding motif, active site and metal binding loop. Phylogenetic analyses of selected amino acid sequence data revealed Tv-RIO1 to be most closely related to the proteins in the species of Caenorhabditis. A structural model of Tv-RIO1 was constructed and compared with the published crystal structure of RIO1 of Archaeoglobus fulgidus (Af-Rio1).
Conclusion: This study provides the first insights into the RIO1 protein kinases of nematodes, and a foundation for further investigations into the biochemical and functional roles of this molecule in biological processes in parasitic nematodes
Yeast prion physiology
2016 Summer.Includes bibliographical references.Prions, or proteinaceous infections, are caused by proteins that have the unique ability to adopt an alternative, self-replicating structure. These self-replicating structures are the causative agent of a number of mammalian diseases including Bovine spongiform encephalopathy, Creutzfeldt-Jakob disease, and Kuru. More recently, yeast were discovered to carry at least a dozen proteins capable of making this structural conversion. Yeast prions are unique in that their prion-forming domains are intrinsically disordered domains, with unusual compositional biases. This thesis addresses two broad questions about yeast prion physiology. First, a recent mutagenic screen suggested that both aromatic and non-aromatic hydrophobic residues strongly promote prion formation. However, while aromatic residues are common in yeast prion domains, non-aromatic hydrophobics are strongly under-represented. The second chapter of this dissertation explores the effects of hydrophobic and aromatic residues on prion formation. Insertion of even a small number of hydrophobic residues is found to strongly increase prion formation. These data, combined with bioinformatics analysis of glutamine/asparagine-rich domains, suggest a limit on the number of strongly prion-promoting residues tolerated in glutamine/asparagine-rich domains. Recent studies have demonstrated that aromatic residues play a key role in the maintenance of yeast prions during cell division. Taken together, these results imply that non-aromatic hydrophobic residues are excluded from prion domains not because they inhibit prion formation, but instead because they too strongly promote aggregation, without promoting prion propagation. Despite more than 20 years of research, we still don’t know why yeast carry so many prion and prion-like domains. It has been proposed that prions may serve some biological function. Chapter Three presents progress on two lines of investigation designed to resolve this issue First, a novel bioinformatics algorithm (GARRF) is used to screen a wide range of proteomes to find examples of Q/N rich domains outside of Saccharomyces cerevisiae. Identifying other species that carry these unusual regions provides insight into their role in cellular biology. We find a wide range species carry prion-like domains at levels comparable to Saccharomyces cerevisiae, and a small number carry up to an order of magnitude more. Second, currently researchers rely primarily on yeast genetic methods to discover and monitor prions. These methods have a number of drawbacks, including a glacially slow readout time. Chapter Three reports on progress towards the development of a novel fluorescence based prion assay. This assay takes advantage of bi-molecular fluorescence complementation, a technique that uses complementary fragments of a fluorescent protein to indicate when two interacting domains are in proximity to one another. When completed, this assay will provide a means to monitor protein aggregations that is both faster and more sensitive than any existing assay
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