Lean implementation to improve scheduling for a multi-cell manufacturing facility

Includes bibliographical references

c-di-GMP modulates type IV MSHA pilus retraction and surface attachment in Vibrio cholerae.

Biofilm formation by Vibrio cholerae facilitates environmental persistence, and hyperinfectivity within the host. Biofilm formation is regulated by 3',5'-cyclic diguanylate (c-di-GMP) and requires production of the type IV mannose-sensitive hemagglutinin (MSHA) pilus. Here, we show that the MSHA pilus is a dynamic extendable and retractable system, and its activity is directly controlled by c-di-GMP. The interaction between c-di-GMP and the ATPase MshE promotes pilus extension, whereas low levels of c-di-GMP correlate with enhanced retraction. Loss of retraction facilitated by the ATPase PilT increases near-surface roaming motility, and impairs initial surface attachment. However, prolonged retraction upon surface attachment results in reduced MSHA-mediated surface anchoring and increased levels of detachment. Our results indicate that c-di-GMP directly controls MshE activity, thus regulating MSHA pilus extension and retraction dynamics, and modulating V. cholerae surface attachment and colonization

A Tissue Engineering product development pathway

Tissue engineering is a field of inquiry and research that uses engineering techniques and principles of biological sciences to develop functional substitutes for reconstruction of damaged organs. Commercial translation of tissue engineering products is currently in progress all over the world. Many companies are moving their interest towards this market segment that grows by 6% per year. Aim of this thesis is to probe the possibility of developing tissue engineering products in the most cost-effective way, minimizing the industrial risk and developing a specific fund raising model. Tissue engineering is based on three main features: cells, scaffolds and bioreactors. Cells are seeded on a scaffold and cultured in a bioreactor in order to obtain a tissue engineering product. Nevertheless, developing cell carrying products is hampered by certification claims ("advanced therapies" certification rules) that unbearably increase R&D and certification costs and can be faced by either big companies or start-ups of big companies and spin-offs of complex aggregates of research centers involved in advanced cell research. On the other hand, scaffolds (certification class IIb) and bioreactors for tissue engineering (certification class I) can be developed with a lower economic effort, being the competition based on innovation, since their market is in the "growth phase" for scaffolds and in the "introduction phase" for bioreactors in the Levitt's product life cycle theory. Purpose of this thesis is to basically study scaffold and bioreactor features, then to preliminarily design some models of bioreactors and, eventually, to set a business model, based on private and public fund raising, aimed to the development of scaffolds for dental implantology and of bioreactors for cardiovascular and bone tissue engineering. Finally, a business plan of a company being spin-off of Politecnico di Torino and industrial start-up has been elaborate

Fine Scale Simulation of Fractured Reservoirs: Applications and Comparison

Imperial Users onl

Floating solar panel park

Treball desenvolupat dins el marc del programa 'European Project Semester'.This Final Report is the culmination of a four month long design study on floating solar panel park feasibility in Vaasa, Finland. The Floating Ideas Team was tasked with coming up with a design that would not only work, but also make a profit. The team focused a lot of time on initial research, an iterative design process, and experiments to gather information that could not be found during the research phase. In this report, one can expect to find the major findings from research in many different areas such as location, panel design, flotation design, cooling techniques, and efficiency adding techniques. The first takeaway is that implementing floating solar parks in Finland would require adding efficiency techniques such as mirrors or concentrators. Second, how the panels are placed means a lot in a location so far north. Placing the panels far away from each other and horizontally will reduce the negative impact of shadows. And third, the rotation of the structure is important in increasing efficiency. Multiple axis tracking is not necessary, but tracking in the vertical axis can add a 50% increase in power generated. This research then lead into the defining of four initial designs which were eventually paired down into one. The largest factors leading to the change in design were the combination of rotation and anchoring methods, the flotation structure, and the structure required hold the panel modules together. In the end, the final design is a modular circular design with panels and mirrors to help add efficiency, approximately 37%. From there, an economic and environmental feasibility study was done and for both, this design was deemed feasible for Finland. With the design, detailed in this report, it would be possible to implement this and make a profit off of it, leading the team to believe that this should be implemented in places looking for alternatives for renewable energy production