Genome Sequences for Three Strains of Kocuria rosea, Including the Type Strain

Genomes from three strains of Kocuria rosea were sequenced. K. rosea ATCC 186, the type strain, was 3,958,612 bp in length with a total G+C content of 72.70%. When assembled, K. rosea ATCC 516 was 3,862,128 bp with a 72.82% G+C content. K. rosea ATCC 49321 was 4,018,783 bp in size with a 72.49% G+C content

An Introduction to Volume 8 of \u3ci\u3eThe New Age\u3c/i\u3e

Dr. Lee Garver\u27s introduction to The New Age, Volume 8 (November 3, 1910 to April 27, 1911)

Mass wasting at the base of the South central Chilean continental margin: the Reloca Slide

Offshore south central Chile (35° S–42° S), the morphology of the lowermost continental slope and trench floor witnesses a voluminous submarine mass-wasting event. The blocky slide body deposited in the Chile Trench at 73°46´ W 35°35´ S was targeted for study during RRS JAMES COOK Cruise JC23 and termed Reloca Slide. Its size of about 24 km3, its steep and high headscarp, the spatial distribution of slide deposits and the cohesive nature of major slide blocks make it interesting to address the issue of tsunami generation. We have obtained seismic reflection data that partly reveal the internal structure of the slide body. Gravity core samples were retrieved that will allow the slide to be dated and linked to the history of sedimentation and slope stability along this particular segment of the Chilean convergent margin. At present we assume a Holocene age for the sliding event

Strengthening HIV Knowledge and Awareness among Undergraduate Students at Historically Black Colleges and Universities

Objective: We describe baseline HIV knowledge among students at historically black colleges and universities (HBCUs) to inform and strengthen HIV education efforts at HBCUs. Methods: We surveyed 1,230 African American HBCU students from 24 HBCUs; 1,051 responses (85.4 %) were analyzable. Results: Although general HIV knowledge was high among respondents (95% of students correctly responded that having sex without a condom constituted unsafe sex), knowledge deficits were noted (only 25% of students reported that multiple sex partners is a form of unsafe sex, while 25% of students reported that withdrawal of the penis before ejaculation reduced HIV risk). Conclusions: Misperceptions about HIV have implications for unintended sexual transmission of HIV. As African American young adults are disproportionately affected by HIV, strengthening HIV prevention efforts at HBCUs may include correcting misperceptions to reduce sexual risk and decrease HIV-related health disparities among young people

Genomic resource development for a diploid mint: Mentha longifolia

This research project aimed to develop genomic resources needed to enable construction of a genetic linkage map of the diploid mint species Mentha longifolia. Such a map would facilitate identification of plant genes involved in resistance to Verticillium fungal infection. For this purpose, a small genomic library was constructed from germplasm accession CMEN 585, 279 genomic inserts were sequenced and annotated and 19 PCR primer pairs were designed and tested on two resistant and two susceptible accessions. The Cleaved Modified Polymorphic Sequence (CAPS) method of molecular marker genotyping was found to detect little variation between crossing parents CMEN 585 (resistant) and CMEN 584 (susceptible). Comparative sequencing of PCR products from two European and two South African accessions revealed greater diversity between than within geographic locations. Future efforts should focus on assessing more sensitive genotyping methods, and developing a mapping population from a cross between European and South African accessions

Association of B-cell Lymphoma Protein-2 and Caspase-3 Expression in Ovarian Cancer

Ovarian cancer remains a major problem of women's health in the world, including Indonesia, and is associated with high rates of incidence and mortality. There are many efforts in early diagnosis on ovarian cancer, but until now there have not been found any satisfactory method. On the other hand, knowledge and research in the field of molecular biology become more advance, one of them is a mechanism to control the growth of cells in ovarian cancer through a process of programmed cell death or apoptosis. B-cell lymphoma protein 2 (Bcl-2) and caspase-3 are proteins that play a role on the mechanism of apoptosis. The purpose of this study was to determine the expression of Bcl-2 and caspase-3 and their association with ovarian cancer. Materials and method: The design of this study was a cross-sectional study. Expression of Bcl-2 and caspase-3 examined by immunohistochemistry under light microscope with 400x light power field and expression as a negative when the protein expressed in 10% or less of cells and as a positive when the protein expressed in more than 10% of cells. A number of 45 subjects were recruited in this study. Thirthy one of 45 subjects showed the expression of Bcl-2 positive (68.9%), while the positive expression of caspase-3 present in 20 subjects (44.4%). There was a significant association between the expression of Bcl-2 with the expression of caspase-3 in ovarian cancer patients (p=0.002; lambda=0.4). There was also a significant association between stage of disease with expression of Bcl-2 (p=0.002; lambda=0.3) dan expression of caspase-3 (p=0.001; lambda=0.3). Conclusion: It concluded that there is a significant association between the expression of Bcl-2 and the expression of caspase-3 in ovarian cancer

The IceCube Realtime Alert System

Although high-energy astrophysical neutrinos were discovered in 2013, their origin is still unknown. Aiming for the identification of an electromagnetic counterpart of a rapidly fading source, we have implemented a realtime analysis framework for the IceCube neutrino observatory. Several analyses selecting neutrinos of astrophysical origin are now operating in realtime at the detector site in Antarctica and are producing alerts for the community to enable rapid follow-up observations. The goal of these observations is to locate the astrophysical objects responsible for these neutrino signals. This paper highlights the infrastructure in place both at the South Pole site and at IceCube facilities in the north that have enabled this fast follow-up program to be implemented. Additionally, this paper presents the first realtime analyses to be activated within this framework, highlights their sensitivities to astrophysical neutrinos and background event rates, and presents an outlook for future discoveries

Semiautomated Skeletonization of the Pulmonary Arterial Tree in Micro-CT Images

We present a simple and robust approach that utilizes planar images at different angular rotations combined with unfiltered back-projection to locate the central axes of the pulmonary arterial tree. Three-dimensional points are selected interactively by the user. The computer calculates a sub- volume unfiltered back-projection orthogonal to the vector connecting the two points and centered on the first point. Because more x-rays are absorbed at the thickest portion of the vessel, in the unfiltered back-projection, the darkest pixel is assumed to be the center of the vessel. The computer replaces this point with the newly computer-calculated point. A second back-projection is calculated around the original point orthogonal to a vector connecting the newly-calculated first point and user-determined second point. The darkest pixel within the reconstruction is determined. The computer then replaces the second point with the XYZ coordinates of the darkest pixel within this second reconstruction. Following a vector based on a moving average of previously determined 3- dimensional points along the vessel\u27s axis, the computer continues this skeletonization process until stopped by the user. The computer estimates the vessel diameter along the set of previously determined points using a method similar to the full width-half max algorithm. On all subsequent vessels, the process works the same way except that at each point, distances between the current point and all previously determined points along different vessels are determined. If the difference is less than the previously estimated diameter, the vessels are assumed to branch. This user/computer interaction continues until the vascular tree has been skeletonized