Machine Learning Based Defect Detection in Robotic Wire Arc Additive Manufacturing

In the last ten years, research interests in various aspects of the Wire Arc Additive Manufacturing (WAAM) processes have grown exponentially. More recently, efforts to integrate an automatic quality assurance system for the WAAM process are increasing. No reliable online monitoring system for the WAAM process is a key gap to be filled for the commercial application of the technology, as it will enable the components produced by the process to be qualified for the relevant standards and hence be fit for use in critical applications in the aerospace or naval sectors. However, most of the existing monitoring methods only detect or solve issues from a specific sensor, no monitoring system integrated with different sensors or data sources is developed in WAAM in the last three years. In addition, complex principles and calculations of conventional algorithms make it hard to be applied in the manufacturing of WAAM as the character of a long manufacturing cycle. Intelligent algorithms provide in-built advantages in processing and analysing data, especially for large datasets generated during the long manufacturing cycles. In this research, in order to establish an intelligent WAAM defect detection system, two intelligent WAAM defect detection modules are developed successfully. The first module takes welding arc current / voltage signals during the deposition process as inputs and uses algorithms such as support vector machine (SVM) and incremental SVM to identify disturbances and continuously learn new defects. The incremental learning module achieved more than a 90% f1-score on new defects. The second module takes CCD images as inputs and uses object detection algorithms to predict the unfused defect during the WAAM manufacturing process with above 72% mAP. This research paves the path for developing an intelligent WAAM online monitoring system in the future. Together with process modelling, simulation and feedback control, it reveals the future opportunity for a digital twin system

Efficient feature reduction and classification methods

Durch die steigende Anzahl verfügbarer Daten in unterschiedlichsten Anwendungsgebieten nimmt der Aufwand vieler Data-Mining Applikationen signifikant zu. Speziell hochdimensionierte Daten (Daten die über viele verschiedene Attribute beschrieben werden) können ein großes Problem für viele Data-Mining Anwendungen darstellen. Neben höheren Laufzeiten können dadurch sowohl für überwachte (supervised), als auch nicht überwachte (unsupervised) Klassifikationsalgorithmen weitere Komplikationen entstehen (z.B. ungenaue Klassifikationsgenauigkeit, schlechte Clustering-Eigenschaften, …). Dies führt zu einem Bedarf an effektiven und effizienten Methoden zur Dimensionsreduzierung. Feature Selection (die Auswahl eines Subsets von Originalattributen) und Dimensionality Reduction (Transformation von Originalattribute in (Linear)-Kombinationen der Originalattribute) sind zwei wichtige Methoden um die Dimension von Daten zu reduzieren. Obwohl sich in den letzten Jahren vielen Studien mit diesen Methoden beschäftigt haben, gibt es immer noch viele offene Fragestellungen in diesem Forschungsgebiet. Darüber hinaus ergeben sich in vielen Anwendungsbereichen durch die immer weiter steigende Anzahl an verfügbaren und verwendeten Attributen und Features laufend neue Probleme. Das Ziel dieser Dissertation ist es, verschiedene Fragenstellungen in diesem Bereich genau zu analysieren und Verbesserungsmöglichkeiten zu entwickeln. Grundsätzlich, werden folgende Ansprüche an Methoden zur Feature Selection und Dimensionality Reduction gestellt: Die Methoden sollten effizient (bezüglich ihres Rechenaufwandes) sein und die resultierenden Feature-Sets sollten die Originaldaten möglichst kompakt repräsentieren können. Darüber hinaus ist es in vielen Anwendungsgebieten wichtig, die Interpretierbarkeit der Originaldaten beizubehalten. Letztendlich sollte der Prozess der Dimensionsreduzierung keinen negativen Effekt auf die Klassifikationsgenauigkeit haben - sondern idealerweise, diese noch verbessern. Offene Problemstellungen in diesem Bereich betreffen unter anderem den Zusammenhang zwischen Methoden zur Dimensionsreduzierung und der resultierenden Klassifikationsgenauigkeit, wobei sowohl eine möglichst kompakte Repräsentation der Daten, als auch eine hohe Klassifikationsgenauigkeit erzielt werden sollen. Wie bereits erwähnt, ergibt sich durch die große Anzahl an Daten auch ein erhöhter Rechenaufwand, weshalb schnelle und effektive Methoden zur Dimensionsreduzierung entwickelt werden müssen, bzw. existierende Methoden verbessert werden müssen. Darüber hinaus sollte natürlich auch der Rechenaufwand der verwendeten Klassifikationsmethoden möglichst gering sein. Des Weiteren ist die Interpretierbarkeit von Feature Sets zwar möglich, wenn Feature Selection Methoden für die Dimensionsreduzierung verwendet werden, im Fall von Dimensionality Reduction sind die resultierenden Feature Sets jedoch meist Linearkombinationen der Originalfeatures. Daher ist es schwierig zu überprüfen, wie viel Information einzelne Originalfeatures beitragen. Im Rahmen dieser Dissertation konnten wichtige Beiträge zu den oben genannten Problemstellungen präsentiert werden: Es wurden neue, effiziente Initialisierungsvarianten für die Dimensionality Reduction Methode Nonnegative Matrix Factorization (NMF) entwickelt, welche im Vergleich zu randomisierter Initialisierung und im Vergleich zu State-of-the-Art Initialisierungsmethoden zu einer schnelleren Reduktion des Approximationsfehlers führen. Diese Initialisierungsvarianten können darüber hinaus mit neu entwickelten und sehr effektiven Klassifikationsalgorithmen basierend auf NMF kombiniert werden. Um die Laufzeit von NMF weiter zu steigern wurden unterschiedliche Varianten von NMF Algorithmen auf Multi-Prozessor Systemen vorgestellt, welche sowohl Task- als auch Datenparallelismus unterstützen und zu einer erheblichen Reduktion der Laufzeit für NMF führen. Außerdem wurde eine effektive Verbesserung der Matlab Implementierung des ALS Algorithmus vorgestellt. Darüber hinaus wurde eine Technik aus dem Bereich des Information Retrieval -- Latent Semantic Indexing -- erfolgreich als Klassifikationsalgorithmus für Email Daten angewendet. Schließlich wurde eine ausführliche empirische Studie über den Zusammenhang verschiedener Feature Reduction Methoden (Feature Selection und Dimensionality Reduction) und der resultierenden Klassifikationsgenauigkeit unterschiedlicher Lernalgorithmen präsentiert. Der starke Einfluss unterschiedlicher Methoden zur Dimensionsreduzierung auf die resultierende Klassifikationsgenauigkeit unterstreicht dass noch weitere Untersuchungen notwendig sind um das komplexe Zusammenspiel von Dimensionsreduzierung und Klassifikation genau analysieren zu können.The sheer volume of data today and its expected growth over the next years are some of the key challenges in data mining and knowledge discovery applications. Besides the huge number of data samples that are collected and processed, the high dimensional nature of data arising in many applications causes the need to develop effective and efficient techniques that are able to deal with this massive amount of data. In addition to the significant increase in the demand of computational resources, those large datasets might also influence the quality of several data mining applications (especially if the number of features is very high compared to the number of samples). As the dimensionality of data increases, many types of data analysis and classification problems become significantly harder. This can lead to problems for both supervised and unsupervised learning. Dimensionality reduction and feature (subset) selection methods are two types of techniques for reducing the attribute space. While in feature selection a subset of the original attributes is extracted, dimensionality reduction in general produces linear combinations of the original attribute set. In both approaches, the goal is to select a low dimensional subset of the attribute space that covers most of the information of the original data. During the last years, feature selection and dimensionality reduction techniques have become a real prerequisite for data mining applications. There are several open questions in this research field, and due to the often increasing number of candidate features for various application areas (e.\,g., email filtering or drug classification/molecular modeling) new questions arise. In this thesis, we focus on some open research questions in this context, such as the relationship between feature reduction techniques and the resulting classification accuracy and the relationship between the variability captured in the linear combinations of dimensionality reduction techniques (e.\,g., PCA, SVD) and the accuracy of machine learning algorithms operating on them. Another important goal is to better understand new techniques for dimensionality reduction, such as nonnegative matrix factorization (NMF), which can be applied for finding parts-based, linear representations of nonnegative data. This ``sum-of-parts'' representation is especially useful if the interpretability of the original data should be retained. Moreover, performance aspects of feature reduction algorithms are investigated. As data grow, implementations of feature selection and dimensionality reduction techniques for high-performance parallel and distributed computing environments become more and more important. In this thesis, we focus on two types of open research questions: methodological advances without any specific application context, and application-driven advances for a specific application context. Summarizing, new methodological contributions are the following: The utilization of nonnegative matrix factorization in the context of classification methods is investigated. In particular, it is of interest how the improved interpretability of NMF factors due to the non-negativity constraints (which is of central importance in various problem settings) can be exploited. Motivated by this problem context two new fast initialization techniques for NMF based on feature selection are introduced. It is shown how approximation accuracy can be increased and/or how computational effort can be reduced compared to standard randomized seeding of the NMF and to state-of-the-art initialization strategies suggested earlier. For example, for a given number of iterations and a required approximation error a speedup of 3.6 compared to standard initialization, and a speedup of 3.4 compared to state-of-the-art initialization strategies could be achieved. Beyond that, novel classification methods based on the NMF are proposed and investigated. We can show that they are not only competitive in terms of classification accuracy with state-of-the-art classifiers, but also provide important advantages in terms of computational effort (especially for low-rank approximations). Moreover, parallelization and distributed execution of NMF is investigated. Several algorithmic variants for efficiently computing NMF on multi-core systems are studied and compared to each other. In particular, several approaches for exploiting task and/or data-parallelism in NMF are studied. We show that for some scenarios new algorithmic variants clearly outperform existing implementations. Last, but not least, a computationally very efficient adaptation of the implementation of the ALS algorithm in Matlab 2009a is investigated. This variant reduces the runtime significantly (in some settings by a factor of 8) and also provides several possibilities to be executed concurrently. In addition to purely methodological questions, we also address questions arising in the adaptation of feature selection and classification methods to two specific application problems: email classification and in silico screening for drug discovery. Different research challenges arise in the contexts of these different application areas, such as the dynamic nature of data for email classification problems, or the imbalance in the number of available samples of different classes for drug discovery problems. Application-driven advances of this thesis comprise the adaptation and application of latent semantic indexing (LSI) to the task of email filtering. Experimental results show that LSI achieves significantly better classification results than the widespread de-facto standard method for this special application context. In the context of drug discovery problems, several groups of well discriminating descriptors could be identified by utilizing the ``sum-of-parts`` representation of NMF. The number of important descriptors could be further increased when applying sparseness constraints on the NMF factors

Cascade of classifier ensembles for reliable medical image classification

Medical image analysis and recognition is one of the most important tools in modern medicine. Different types of imaging technologies such as X-ray, ultrasonography, biopsy, computed tomography and optical coherence tomography have been widely used in clinical diagnosis for various kinds of diseases. However, in clinical applications, it is usually time consuming to examine an image manually. Moreover, there is always a subjective element related to the pathological examination of an image. This produces the potential risk of a doctor to make a wrong decision. Therefore, an automated technique will provide valuable assistance for physicians. By utilizing techniques from machine learning and image analysis, this thesis aims to construct reliable diagnostic models for medical image data so as to reduce the problems faced by medical experts in image examination. Through supervised learning of the image data, the diagnostic model can be constructed automatically. The process of image examination by human experts is very difficult to simulate, as the knowledge of medical experts is often fuzzy and not easy to be quantified. Therefore, the problem of automatic diagnosis based on images is usually converted to the problem of image classification. For the image classification tasks, using a single classifier is often hard to capture all aspects of image data distributions. Therefore, in this thesis, a classifier ensemble based on random subspace method is proposed to classify microscopic images. The multi-layer perceptrons are used as the base classifiers in the ensemble. Three types of feature extraction methods are selected for microscopic image description. The proposed method was evaluated on two microscopic image sets and showed promising results compared with the state-of-art results. In order to address the classification reliability in biomedical image classification problems, a novel cascade classification system is designed. Two random subspace based classifier ensembles are serially connected in the proposed system. In the first stage of the cascade system, an ensemble of support vector machines are used as the base classifiers. The second stage consists of a neural network classifier ensemble. Using the reject option, the images whose classification results cannot achieve the predefined rejection threshold at the current stage will be passed to the next stage for further consideration. The proposed cascade system was evaluated on a breast cancer biopsy image set and two UCI machine learning datasets, the experimental results showed that the proposed method can achieve high classification reliability and accuracy with small rejection rate. Many computer aided diagnosis systems face the problem of imbalance data. The datasets used for diagnosis are often imbalanced as the number of normal cases is usually larger than the number of the disease cases. Classifiers that generalize over the data are not the most appropriate choice in such an imbalanced situation. To tackle this problem, a novel one-class classifier ensemble is proposed. The Kernel Principle Components are selected as the base classifiers in the ensemble; the base classifiers are trained by different types of image features respectively and then combined using a product combining rule. The proposed one-class classifier ensemble is also embedded into the cascade scheme to improve classification reliability and accuracy. The proposed method was evaluated on two medical image sets. Favorable results were obtained comparing with the state-of-art results

QUIS-CAMPI: Biometric Recognition in Surveillance Scenarios

The concerns about individuals security have justified the increasing number of surveillance cameras deployed both in private and public spaces. However, contrary to popular belief, these devices are in most cases used solely for recording, instead of feeding intelligent analysis processes capable of extracting information about the observed individuals. Thus, even though video surveillance has already proved to be essential for solving multiple crimes, obtaining relevant details about the subjects that took part in a crime depends on the manual inspection of recordings. As such, the current goal of the research community is the development of automated surveillance systems capable of monitoring and identifying subjects in surveillance scenarios. Accordingly, the main goal of this thesis is to improve the performance of biometric recognition algorithms in data acquired from surveillance scenarios. In particular, we aim at designing a visual surveillance system capable of acquiring biometric data at a distance (e.g., face, iris or gait) without requiring human intervention in the process, as well as devising biometric recognition methods robust to the degradation factors resulting from the unconstrained acquisition process. Regarding the first goal, the analysis of the data acquired by typical surveillance systems shows that large acquisition distances significantly decrease the resolution of biometric samples, and thus their discriminability is not sufficient for recognition purposes. In the literature, diverse works point out Pan Tilt Zoom (PTZ) cameras as the most practical way for acquiring high-resolution imagery at a distance, particularly when using a master-slave configuration. In the master-slave configuration, the video acquired by a typical surveillance camera is analyzed for obtaining regions of interest (e.g., car, person) and these regions are subsequently imaged at high-resolution by the PTZ camera. Several methods have already shown that this configuration can be used for acquiring biometric data at a distance. Nevertheless, these methods failed at providing effective solutions to the typical challenges of this strategy, restraining its use in surveillance scenarios. Accordingly, this thesis proposes two methods to support the development of a biometric data acquisition system based on the cooperation of a PTZ camera with a typical surveillance camera. The first proposal is a camera calibration method capable of accurately mapping the coordinates of the master camera to the pan/tilt angles of the PTZ camera. The second proposal is a camera scheduling method for determining - in real-time - the sequence of acquisitions that maximizes the number of different targets obtained, while minimizing the cumulative transition time. In order to achieve the first goal of this thesis, both methods were combined with state-of-the-art approaches of the human monitoring field to develop a fully automated surveillance capable of acquiring biometric data at a distance and without human cooperation, designated as QUIS-CAMPI system. The QUIS-CAMPI system is the basis for pursuing the second goal of this thesis. The analysis of the performance of the state-of-the-art biometric recognition approaches shows that these approaches attain almost ideal recognition rates in unconstrained data. However, this performance is incongruous with the recognition rates observed in surveillance scenarios. Taking into account the drawbacks of current biometric datasets, this thesis introduces a novel dataset comprising biometric samples (face images and gait videos) acquired by the QUIS-CAMPI system at a distance ranging from 5 to 40 meters and without human intervention in the acquisition process. This set allows to objectively assess the performance of state-of-the-art biometric recognition methods in data that truly encompass the covariates of surveillance scenarios. As such, this set was exploited for promoting the first international challenge on biometric recognition in the wild. This thesis describes the evaluation protocols adopted, along with the results obtained by the nine methods specially designed for this competition. In addition, the data acquired by the QUIS-CAMPI system were crucial for accomplishing the second goal of this thesis, i.e., the development of methods robust to the covariates of surveillance scenarios. The first proposal regards a method for detecting corrupted features in biometric signatures inferred by a redundancy analysis algorithm. The second proposal is a caricature-based face recognition approach capable of enhancing the recognition performance by automatically generating a caricature from a 2D photo. The experimental evaluation of these methods shows that both approaches contribute to improve the recognition performance in unconstrained data.A crescente preocupação com a segurança dos indivíduos tem justificado o crescimento do número de câmaras de vídeo-vigilância instaladas tanto em espaços privados como públicos. Contudo, ao contrário do que normalmente se pensa, estes dispositivos são, na maior parte dos casos, usados apenas para gravação, não estando ligados a nenhum tipo de software inteligente capaz de inferir em tempo real informações sobre os indivíduos observados. Assim, apesar de a vídeo-vigilância ter provado ser essencial na resolução de diversos crimes, o seu uso está ainda confinado à disponibilização de vídeos que têm que ser manualmente inspecionados para extrair informações relevantes dos sujeitos envolvidos no crime. Como tal, atualmente, o principal desafio da comunidade científica é o desenvolvimento de sistemas automatizados capazes de monitorizar e identificar indivíduos em ambientes de vídeo-vigilância. Esta tese tem como principal objetivo estender a aplicabilidade dos sistemas de reconhecimento biométrico aos ambientes de vídeo-vigilância. De forma mais especifica, pretende-se 1) conceber um sistema de vídeo-vigilância que consiga adquirir dados biométricos a longas distâncias (e.g., imagens da cara, íris, ou vídeos do tipo de passo) sem requerer a cooperação dos indivíduos no processo; e 2) desenvolver métodos de reconhecimento biométrico robustos aos fatores de degradação inerentes aos dados adquiridos por este tipo de sistemas. No que diz respeito ao primeiro objetivo, a análise aos dados adquiridos pelos sistemas típicos de vídeo-vigilância mostra que, devido à distância de captura, os traços biométricos amostrados não são suficientemente discriminativos para garantir taxas de reconhecimento aceitáveis. Na literatura, vários trabalhos advogam o uso de câmaras Pan Tilt Zoom (PTZ) para adquirir imagens de alta resolução à distância, principalmente o uso destes dispositivos no modo masterslave. Na configuração master-slave um módulo de análise inteligente seleciona zonas de interesse (e.g. carros, pessoas) a partir do vídeo adquirido por uma câmara de vídeo-vigilância e a câmara PTZ é orientada para adquirir em alta resolução as regiões de interesse. Diversos métodos já mostraram que esta configuração pode ser usada para adquirir dados biométricos à distância, ainda assim estes não foram capazes de solucionar alguns problemas relacionados com esta estratégia, impedindo assim o seu uso em ambientes de vídeo-vigilância. Deste modo, esta tese propõe dois métodos para permitir a aquisição de dados biométricos em ambientes de vídeo-vigilância usando uma câmara PTZ assistida por uma câmara típica de vídeo-vigilância. O primeiro é um método de calibração capaz de mapear de forma exata as coordenadas da câmara master para o ângulo da câmara PTZ (slave) sem o auxílio de outros dispositivos óticos. O segundo método determina a ordem pela qual um conjunto de sujeitos vai ser observado pela câmara PTZ. O método proposto consegue determinar em tempo-real a sequência de observações que maximiza o número de diferentes sujeitos observados e simultaneamente minimiza o tempo total de transição entre sujeitos. De modo a atingir o primeiro objetivo desta tese, os dois métodos propostos foram combinados com os avanços alcançados na área da monitorização de humanos para assim desenvolver o primeiro sistema de vídeo-vigilância completamente automatizado e capaz de adquirir dados biométricos a longas distâncias sem requerer a cooperação dos indivíduos no processo, designado por sistema QUIS-CAMPI. O sistema QUIS-CAMPI representa o ponto de partida para iniciar a investigação relacionada com o segundo objetivo desta tese. A análise do desempenho dos métodos de reconhecimento biométrico do estado-da-arte mostra que estes conseguem obter taxas de reconhecimento quase perfeitas em dados adquiridos sem restrições (e.g., taxas de reconhecimento maiores do que 99% no conjunto de dados LFW). Contudo, este desempenho não é corroborado pelos resultados observados em ambientes de vídeo-vigilância, o que sugere que os conjuntos de dados atuais não contêm verdadeiramente os fatores de degradação típicos dos ambientes de vídeo-vigilância. Tendo em conta as vulnerabilidades dos conjuntos de dados biométricos atuais, esta tese introduz um novo conjunto de dados biométricos (imagens da face e vídeos do tipo de passo) adquiridos pelo sistema QUIS-CAMPI a uma distância máxima de 40m e sem a cooperação dos sujeitos no processo de aquisição. Este conjunto permite avaliar de forma objetiva o desempenho dos métodos do estado-da-arte no reconhecimento de indivíduos em imagens/vídeos capturados num ambiente real de vídeo-vigilância. Como tal, este conjunto foi utilizado para promover a primeira competição de reconhecimento biométrico em ambientes não controlados. Esta tese descreve os protocolos de avaliação usados, assim como os resultados obtidos por 9 métodos especialmente desenhados para esta competição. Para além disso, os dados adquiridos pelo sistema QUIS-CAMPI foram essenciais para o desenvolvimento de dois métodos para aumentar a robustez aos fatores de degradação observados em ambientes de vídeo-vigilância. O primeiro é um método para detetar características corruptas em assinaturas biométricas através da análise da redundância entre subconjuntos de características. O segundo é um método de reconhecimento facial baseado em caricaturas automaticamente geradas a partir de uma única foto do sujeito. As experiências realizadas mostram que ambos os métodos conseguem reduzir as taxas de erro em dados adquiridos de forma não controlada

Texture and Colour in Image Analysis

Research in colour and texture has experienced major changes in the last few years. This book presents some recent advances in the field, specifically in the theory and applications of colour texture analysis. This volume also features benchmarks, comparative evaluations and reviews

Smart Monitoring and Control in the Future Internet of Things

The Internet of Things (IoT) and related technologies have the promise of realizing pervasive and smart applications which, in turn, have the potential of improving the quality of life of people living in a connected world. According to the IoT vision, all things can cooperate amongst themselves and be managed from anywhere via the Internet, allowing tight integration between the physical and cyber worlds and thus improving efficiency, promoting usability, and opening up new application opportunities. Nowadays, IoT technologies have successfully been exploited in several domains, providing both social and economic benefits. The realization of the full potential of the next generation of the Internet of Things still needs further research efforts concerning, for instance, the identification of new architectures, methodologies, and infrastructures dealing with distributed and decentralized IoT systems; the integration of IoT with cognitive and social capabilities; the enhancement of the sensing–analysis–control cycle; the integration of consciousness and awareness in IoT environments; and the design of new algorithms and techniques for managing IoT big data. This Special Issue is devoted to advancements in technologies, methodologies, and applications for IoT, together with emerging standards and research topics which would lead to realization of the future Internet of Things

Deep learning for land cover and land use classification

Recent advances in sensor technologies have witnessed a vast amount of very fine spatial resolution (VFSR) remotely sensed imagery being collected on a daily basis. These VFSR images present fine spatial details that are spectrally and spatially complicated, thus posing huge challenges in automatic land cover (LC) and land use (LU) classification. Deep learning reignited the pursuit of artificial intelligence towards a general purpose machine to be able to perform any human-related tasks in an automated fashion. This is largely driven by the wave of excitement in deep machine learning to model the high-level abstractions through hierarchical feature representations without human-designed features or rules, which demonstrates great potential in identifying and characterising LC and LU patterns from VFSR imagery. In this thesis, a set of novel deep learning methods are developed for LC and LU image classification based on the deep convolutional neural networks (CNN) as an example. Several difficulties, however, are encountered when trying to apply the standard pixel-wise CNN for LC and LU classification using VFSR images, including geometric distortions, boundary uncertainties and huge computational redundancy. These technical challenges for LC classification were solved either using rule-based decision fusion or through uncertainty modelling using rough set theory. For land use, an object-based CNN method was proposed, in which each segmented object (a group of homogeneous pixels) was sampled and predicted by CNN with both within-object and between-object information. LU was, thus, classified with high accuracy and efficiency. Both LC and LU formulate a hierarchical ontology at the same geographical space, and such representations are modelled by their joint distribution, in which LC and LU are classified simultaneously through iteration. These developed deep learning techniques achieved by far the highest classification accuracy for both LC and LU, up to around 90% accuracy, about 5% higher than the existing deep learning methods, and 10% greater than traditional pixel-based and object-based approaches. This research made a significant contribution in LC and LU classification through deep learning based innovations, and has great potential utility in a wide range of geospatial applications

Computer aided classification of histopathological damage in images of haematoxylin and eosin stained human skin

EngD ThesisExcised human skin can be used as a model to assess the potency, immunogenicity and contact sensitivity of potential therapeutics or cosmetics via the assessment of histological damage. The current method of assessing the damage uses traditional manual histological assessment, which is inherently subjective, time consuming and prone to intra-observer variability. Computer aided analysis has the potential to address issues surrounding traditional histological techniques through the application of quantitative analysis. This thesis describes the development of a computer aided process to assess the immune-mediated structural breakdown of human skin tissue. Research presented includes assessment and optimisation of image acquisition methodologies, development of an image processing and segmentation algorithm, identification and extraction of a novel set of descriptive image features and the evaluation of a selected subset of these features in a classification model. A new segmentation method is presented to identify epidermis tissue from skin with varying degrees of histopathological damage. Combining enhanced colour information with general image intensity information, the fully automated methodology segments the epidermis with a mean specificity of 97.7%, a mean sensitivity of 89.4% and a mean accuracy of 96.5% and segments effectively for different severities of tissue damage. A set of 140 feature measurements containing information about the tissue changes associated with different grades of histopathological skin damage were identified and a wrapper algorithm employed to select a subset of the extracted features, evaluating feature subsets based their prediction error for an independent test set in a Naïve Bayes Classifier. The final classification algorithm classified a 169 image set with an accuracy of 94.1%, of these images 20 were an unseen validation set for which the accuracy was 85.0%. The final classification method has a comparable accuracy to the existing manual method, improved repeatability and reproducibility and does not require an experienced histopathologist