13,725 research outputs found
Meso-scale FDM material layout design strategies under manufacturability constraints and fracture conditions
In the manufacturability-driven design (MDD) perspective, manufacturability of the product or system is the most important of the design requirements. In addition to being able to ensure that complex designs (e.g., topology optimization) are manufacturable with a given process or process family, MDD also helps mechanical designers to take advantage of unique process-material effects generated during manufacturing. One of the most recognizable examples of this comes from the scanning-type family of additive manufacturing (AM) processes; the most notable and familiar member of this family is the fused deposition modeling (FDM) or fused filament fabrication (FFF) process. This process works by selectively depositing uniform, approximately isotropic beads or elements of molten thermoplastic material (typically structural engineering plastics) in a series of pre-specified traces to build each layer of the part. There are many interesting 2-D and 3-D mechanical design problems that can be explored by designing the layout of these elements. The resulting structured, hierarchical material (which is both manufacturable and customized layer-by-layer within the limits of the process and material) can be defined as a manufacturing process-driven structured material (MPDSM). This dissertation explores several practical methods for designing these element layouts for 2-D and 3-D meso-scale mechanical problems, focusing ultimately on design-for-fracture. Three different fracture conditions are explored: (1) cases where a crack must be prevented or stopped, (2) cases where the crack must be encouraged or accelerated, and (3) cases where cracks must grow in a simple pre-determined pattern. Several new design tools, including a mapping method for the FDM manufacturability constraints, three major literature reviews, the collection, organization, and analysis of several large (qualitative and quantitative) multi-scale datasets on the fracture behavior of FDM-processed materials, some new experimental equipment, and the refinement of a fast and simple g-code generator based on commercially-available software, were developed and refined to support the design of MPDSMs under fracture conditions. The refined design method and rules were experimentally validated using a series of case studies (involving both design and physical testing of the designs) at the end of the dissertation. Finally, a simple design guide for practicing engineers who are not experts in advanced solid mechanics nor process-tailored materials was developed from the results of this project.U of I OnlyAuthor's request
Pollution-induced community tolerance in freshwater biofilms – from molecular mechanisms to loss of community functions
Exposure to herbicides poses a threat to aquatic biofilms by affecting their community structure, physiology and function. These changes render biofilms to become more tolerant, but on the downside community tolerance has ecologic costs. A concept that addresses induced community tolerance to a pollutant (PICT) was introduced by Blanck and Wängberg (1988). The basic principle of the concept is that microbial communities undergo pollution-induced succession when exposed to a pollutant over a long period of time, which changes communities structurally and functionally and enhancing tolerance to the pollutant exposure. However, the mechanisms of tolerance and the ecologic consequences were hardly studied up to date. This thesis addresses the structural and functional changes in biofilm communities and applies modern molecular methods to unravel molecular tolerance mechanisms.
Two different freshwater biofilm communities were cultivated for a period of five weeks, with one of the communities being contaminated with 4 μg L-1 diuron. Subsequently, the communities were characterized for structural and functional differences, especially focusing on their crucial role of photosynthesis. The community structure of the autotrophs was assessed using HPLC-based pigment analysis and their functional alterations were investigated using Imaging-PAM fluorometry to study photosynthesis and community oxygen profiling to determine net primary production. Then, the molecular fingerprints of the communities were measured with meta-transcriptomics (RNA-Seq) and GC-based community metabolomics approaches and analyzed with respect to changes in their molecular functions. The communities were acute exposed to diuron for one hour in a dose-response design, to reveal a potential PICT and uncover related adaptation to diuron exposure. The combination of apical and molecular methods in a dose-response design enabled the linkage of functional effects of diuron exposure and underlying molecular mechanisms based on a sensitivity analysis.
Chronic exposure to diuron impaired freshwater biofilms in their biomass accrual. The contaminated communities particularly lost autotrophic biomass, reflected by the decrease in specific chlorophyll a content. This loss was associated with a change in the molecular fingerprint of the communities, which substantiates structural and physiological changes. The decline in autotrophic biomass could be due to a primary loss of sensitive autotrophic organisms caused by the selection of better adapted species in the course of chronic exposure. Related to this hypothesis, an increase in diuron tolerance has been detected in the contaminated communities and molecular mechanisms facilitating tolerance have been found. It was shown that genes of the photosystem, reductive-pentose phosphate cycle and arginine metabolism were differentially expressed among the communities and that an increased amount of potential antioxidant degradation products was found in the contaminated communities. This led to the hypothesis that contaminated communities may have adapted to oxidative stress, making them less sensitive to diuron exposure. Moreover, the photosynthetic light harvesting complex was altered and the photoprotective xanthophyll cycle was increased in the contaminated communities. Despite these adaptation strategies, the loss of autotrophic biomass has been shown to impair primary production. This impairment persisted even under repeated short-term exposure, so that the tolerance mechanisms cannot safeguard primary production as a key function in aquatic systems.:1. The effect of chemicals on organisms and their functions .............................. 1
1.1 Welcome to the anthropocene .......................................................................... 1
1.2 From cellular stress responses to ecosystem resilience ................................... 3
1.2.1 The individual pursuit for homeostasis ....................................................... 3
1.2.2 Stability from diversity ................................................................................. 5
1.3 Community ecotoxicology - a step forward in monitoring the effects of chemical
pollution? ................................................................................................................. 6
1.4 Functional ecotoxicological assessment of microbial communities ................... 9
1.5 Molecular tools – the key to a mechanistic understanding of stressor effects
from a functional perspective in microbial communities? ...................................... 12
2. Aims and Hypothesis ......................................................................................... 14
2.1 Research question .......................................................................................... 14
2.2 Hypothesis and outline .................................................................................... 15
2.3 Experimental approach & concept .................................................................. 16
2.3.1 Aquatic freshwater biofilms as model community ..................................... 16
2.3.2 Diuron as model herbicide ........................................................................ 17
2.3.3 Experimental design ................................................................................. 18
3. Structural and physiological changes in microbial communities after chronic
exposure - PICT and altered functional capacity ................................................. 21
3.1 Introduction ..................................................................................................... 21
3.2 Methods .......................................................................................................... 23
3.2.1 Biofilm cultivation ...................................................................................... 23
3.2.2 Dry weight and autotrophic index ............................................................. 23
3.2.4 Pigment analysis of periphyton ................................................................. 23
3.2.4.1 In-vivo pigment analysis for community characterization ....................... 24
3.2.4.2 In-vivo pigment analysis based on Imaging-PAM fluorometry ............... 24
3.2.4.3 In-vivo pigment fluorescence for tolerance detection ............................. 26
3.2.4.4 Ex-vivo pigment analysis by high-pressure liquid-chromatography ....... 27
3.2.5 Community oxygen metabolism measurements ....................................... 28
3.3 Results and discussion ................................................................................... 29
3.3.1 Comparison of the structural community parameters ............................... 29
3.3.2 Photosynthetic activity and primary production of the communities after
selection phase ................................................................................................. 33
3.3.3 Acquisition of photosynthetic tolerance .................................................... 34
3.3.4 Primary production at exposure conditions ............................................... 36
3.3.5 Tolerance detection in primary production ................................................ 37
3.4 Summary and Conclusion ........................................................................... 40
4. Community gene expression analysis by meta-transcriptomics ................... 41
4.1 Introduction to meta-transcriptomics ............................................................... 41
4.2. Methods ......................................................................................................... 43
4.2.1 Sampling and RNA extraction................................................................... 43
4.2.2 RNA sequencing analysis ......................................................................... 44
4.2.3 Data assembly and processing................................................................. 45
4.2.4 Prioritization of contigs and annotation ..................................................... 47
4.2.5 Sensitivity analysis of biological processes .............................................. 48
4.3 Results and discussion ................................................................................... 48
4.3.1 Characterization of the meta-transcriptomic fingerprints .......................... 49
4.3.2 Insights into community stress response mechanisms using trend analysis
(DRomic’s) ......................................................................................................... 51
4.3.3 Response pattern in the isoform PS genes .............................................. 63
4.5 Summary and conclusion ................................................................................ 65
5. Community metabolome analysis ..................................................................... 66
5.1 Introduction to community metabolomics ........................................................ 66
5.2 Methods .......................................................................................................... 68
5.2.1 Sampling, metabolite extraction and derivatisation................................... 68
5.2.2 GC-TOF-MS analysis ............................................................................... 69
5.2.3 Data processing and statistical analysis ................................................... 69
5.3 Results and discussion ................................................................................... 70
5.3.1 Characterization of the metabolic fingerprints .......................................... 70
5.3.2 Difference in the metabolic fingerprints .................................................... 71
5.3.3 Differential metabolic responses of the communities to short-term exposure
of diuron ............................................................................................................ 73
5.4 Summary and conclusion ................................................................................ 78
6. Synthesis ............................................................................................................. 79
6.1 Approaches and challenges for linking molecular data to functional
measurements ...................................................................................................... 79
6.2 Methods .......................................................................................................... 83
6.2.1 Summary on the data ............................................................................... 83
6.2.2 Aggregation of molecular data to index values (TELI and MELI) .............. 83
6.2.3 Functional annotation of contigs and metabolites using KEGG ................ 83
6.3 Results and discussion ................................................................................... 85
6.3.1 Results of aggregation techniques ........................................................... 85
6.3.2 Sensitivity analysis of the different molecular approaches and endpoints 86
6.3.3 Mechanistic view of the molecular stress responses based on KEGG
functions ............................................................................................................ 89
6.4 Consolidation of the results – holistic interpretation and discussion ............... 93
6.4.1 Adaptation to chronic diuron exposure - from molecular changes to
community effects.............................................................................................. 93
6.4.2 Assessment of the ecological costs of Pollution-induced community
tolerance based on primary production ............................................................. 94
6.5 Outlook ............................................................................................................ 9
NIRwave: A wave-turbulence-driven solar wind model constrained by PSP observations
We generate a model description of the solar wind based on an explicit
wave-turbulence-driven heating mechanism, and constrain our model with
observational data. We included an explicit coronal heating source term in the
general 3D magnetohydrodynamic code NIRVANA to simulate the properties of the
solar wind. The adapted heating mechanism is based on the interaction and
subsequent dissipation of counter-propagating Alfv\'en waves in the solar
corona, accounting for a turbulent heating rate Q_p. The solar magnetic field
is assumed to be an axisymmetric dipole with a field strength of 1 G. Our model
results are validated against observational data taken by the Parker Solar
Probe (PSP). Our NIRwave solar wind model reconstructs the bimodal structure of
the solar wind with slow and fast wind speeds of 410 km/s and 650 km/s
respectively. The global mass-loss rate of our solar wind model is 2.6e-14
solar masses per year. Despite implementing simplified conditions to represent
the solar magnetic field, the solar wind parameters characterising our
steady-state solution are in reasonable agreement with previously established
results and empirical constraints. The number density from our wind solution is
in good agreement with the derived empirical constraints, with larger
deviations for the radial velocity and temperature. In a comparison to a
polytropic wind model generated with NIRVANA, we find that our NIRwave model is
in better agreement with the observational constraints that we derive.Comment: 14 pages, 12 figures, accepted for publication in A&
Anuário científico da Escola Superior de Tecnologia da Saúde de Lisboa - 2021
É com grande prazer que apresentamos a mais recente edição (a 11.ª) do Anuário Científico da Escola Superior de Tecnologia da Saúde de Lisboa. Como instituição de ensino superior, temos o compromisso de promover e incentivar a pesquisa científica em todas as áreas do conhecimento que contemplam a nossa missão. Esta publicação tem como objetivo divulgar toda a produção científica desenvolvida pelos Professores, Investigadores, Estudantes e Pessoal não Docente da ESTeSL durante 2021. Este Anuário é, assim, o reflexo do trabalho árduo e dedicado da nossa comunidade, que se empenhou na produção de conteúdo científico de elevada qualidade e partilhada com a Sociedade na forma de livros, capítulos de livros, artigos publicados em revistas nacionais e internacionais, resumos de comunicações orais e pósteres, bem como resultado dos trabalhos de 1º e 2º ciclo. Com isto, o conteúdo desta publicação abrange uma ampla variedade de tópicos, desde temas mais fundamentais até estudos de aplicação prática em contextos específicos de Saúde, refletindo desta forma a pluralidade e diversidade de áreas que definem, e tornam única, a ESTeSL. Acreditamos que a investigação e pesquisa científica é um eixo fundamental para o desenvolvimento da sociedade e é por isso que incentivamos os nossos estudantes a envolverem-se em atividades de pesquisa e prática baseada na evidência desde o início dos seus estudos na ESTeSL. Esta publicação é um exemplo do sucesso desses esforços, sendo a maior de sempre, o que faz com que estejamos muito orgulhosos em partilhar os resultados e descobertas dos nossos investigadores com a comunidade científica e o público em geral. Esperamos que este Anuário inspire e motive outros estudantes, profissionais de saúde, professores e outros colaboradores a continuarem a explorar novas ideias e contribuir para o avanço da ciência e da tecnologia no corpo de conhecimento próprio das áreas que compõe a ESTeSL. Agradecemos a todos os envolvidos na produção deste anuário e desejamos uma leitura inspiradora e agradável.info:eu-repo/semantics/publishedVersio
Neuroanatomical and gene expression features of the rabbit accessory olfactory system. Implications of pheromone communication in reproductive behaviour and animal physiology
Mainly driven by the vomeronasal system (VNS), pheromone
communication is involved in many species-specific fundamental innate socio-sexual behaviors such as mating and
fighting, which are essential for animal reproduction and survival. Rabbits are a unique model for studying
chemocommunication due to the discovery of the rabbit mammary pheromone, but paradoxically there has been a
lack of knowledge regarding its VNS pathway. In this work, we aim at filling this gap by approaching the system
from an integrative point of view, providing extensive anatomical and genomic data of the rabbit VNS, as well as
pheromone-mediated reproductive and behavioural studies. Our results build strong foundation for further
translational studies which aim at implementing the use of pheromones to improve animal production and welfare
Lift EVERY Voice and Sing: An Intersectional Qualitative Study Examining the Experiences of Lesbian, Gay, Bisexual, and Queer Faculty and Administrators at Historically Black Colleges and Universities
While there is minimal literature that address the experiences of lesbian, gay, bisexual, and trans* identified students at Historically Black Colleges and Universities (HBCUs), the experiences of Black, queer faculty and administrators at HBCUs has not been studied. This intersectional qualitative research study focused on the experiences of lesbian, gay, bisexual, and queer identified faculty and administrators who work at HBCUs. By investigating the intersections of religion, race, gender, and sexuality within a predominantly Black institution, this study aims to enhance diversity, equity, and inclusion efforts at HBCUs by sharing the experiences of the LGBQ faculty and administrators that previously or currently work at an HBCU as a full-time employee. The research questions that guided this study were 1) How have LGBQ faculty and staff negotiated/navigated their careers at HBCUs? and 2) How do LGBQ faculty and staff at HBCUs influence cultural (relating to LGBQ inclusion) change at the organizational level? The main theoretical framework used was intersectionality and it shaped the chosen methodology and methods. The Politics of Respectability was the second theoretical framework used to describe the intra-racial tensions within the Black/African American community. The study included 60-120 minute interviews with 12 participants. Using intersectionality as a guide, the data were coded and utilized for thematic analysis. Then, an ethnodramatic performance engages readers. The goals of this study were to encourage policy changes, promote inclusivity for LGBQ employees at HBCUs, and provide an expansion to the body of literature in the field pertaining to the experiences of LGBQ faculty and administrators in higher education
Gasificação direta de biomassa para produção de gás combustível
The excessive consumption of fossil fuels to satisfy the world necessities of
energy and commodities led to the emission of large amounts of greenhouse
gases in the last decades, contributing significantly to the greatest
environmental threat of the 21st century: Climate Change. The answer to this
man-made disaster is not simple and can only be made if distinct stakeholders
and governments are brought to cooperate and work together. This is
mandatory if we want to change our economy to one more sustainable and
based in renewable materials, and whose energy is provided by the eternal
nature energies (e.g., wind, solar). In this regard, biomass can have a main role
as an adjustable and renewable feedstock that allows the replacement of fossil
fuels in various applications, and the conversion by gasification allows the
necessary flexibility for that purpose. In fact, fossil fuels are just biomass that
underwent extreme pressures and heat for millions of years. Furthermore,
biomass is a resource that, if not used or managed, increases wildfire risks.
Consequently, we also have the obligation of valorizing and using this
resource.
In this work, it was obtained new scientific knowledge to support the
development of direct (air) gasification of biomass in bubbling fluidized bed
reactors to obtain a fuel gas with suitable properties to replace natural gas in
industrial gas burners. This is the first step for the integration and development
of gasification-based biorefineries, which will produce a diverse number of
value-added products from biomass and compete with current petrochemical
refineries in the future. In this regard, solutions for the improvement of the raw
producer gas quality and process efficiency parameters were defined and
analyzed. First, addition of superheated steam as primary measure allowed the
increase of H2 concentration and H2/CO molar ratio in the producer gas without
compromising the stability of the process. However, the measure mainly
showed potential for the direct (air) gasification of high-density biomass (e.g.,
pellets), due to the necessity of having char accumulation in the reactor bottom
bed for char-steam reforming reactions. Secondly, addition of refused derived
fuel to the biomass feedstock led to enhanced gasification products, revealing
itself as a highly promising strategy in terms of economic viability and
environmental benefits of future gasification-based biorefineries, due to the
high availability and low costs of wastes. Nevertheless, integrated techno economic and life cycle analyses must be performed to fully characterize the
process. Thirdly, application of low-cost catalyst as primary measure revealed
potential by allowing the improvement of the producer gas quality (e.g., H2 and
CO concentration, lower heating value) and process efficiency parameters with
distinct solid materials; particularly, the application of concrete, synthetic
fayalite and wood pellets chars, showed promising results. Finally, the
economic viability of the integration of direct (air) biomass gasification
processes in the pulp and paper industry was also shown, despite still lacking
interest to potential investors. In this context, the role of government policies
and appropriate economic instruments are of major relevance to increase the
implementation of these projects.O consumo excessivo de combustíveis fósseis para garantir as necessidades e
interesses da sociedade conduziu à emissão de elevadas quantidades de
gases com efeito de estufa nas últimas décadas, contribuindo
significativamente para a maior ameaça ambiental do século XXI: Alterações
Climáticas. A solução para este desastre de origem humana é de caráter
complexo e só pode ser atingida através da cooperação de todos os governos
e partes interessadas. Para isto, é obrigatória a criação de uma bioeconomia
como base de um futuro mais sustentável, cujas necessidades energéticas e
materiais sejam garantidas pelas eternas energias da natureza (e.g., vento,
sol). Neste sentido, a biomassa pode ter um papel principal como uma matéria prima ajustável e renovável que permite a substituição de combustíveis fósseis
num variado número de aplicações, e a sua conversão através da gasificação
pode ser a chave para este propósito. Afinal, na prática, os combustíveis
fósseis são apenas biomassa sujeita a elevada temperatura e pressão durante
milhões de anos. Além do mais, a gestão eficaz da biomassa é fundamental
para a redução dos riscos de incêndio florestal e, como tal, temos o dever de
utilizar e valorizar este recurso.
Neste trabalho, foi obtido novo conhecimento científico para suporte do
desenvolvimento das tecnologias de gasificação direta (ar) de biomassa em
leitos fluidizados borbulhantes para produção de gás combustível, com o
objetivo da substituição de gás natural em queimadores industriais. Este é o
primeiro passo para o desenvolvimento de biorrefinarias de gasificação, uma
potencial futura indústria que irá providenciar um variado número de produtos
de valor acrescentado através da biomassa e competir com a atual indústria
petroquímica. Neste sentido, foram analisadas várias medidas para a melhoria
da qualidade do gás produto bruto e dos parâmetros de eficiência do processo.
Em primeiro, a adição de vapor sobreaquecido como medida primária permitiu
o aumento da concentração de H2 e da razão molar H2/CO no gás produto sem
comprometer a estabilidade do processo. No entanto, esta medida somente
revelou potencial para a gasificação direta (ar) de biomassa de alta densidade
(e.g., pellets) devido à necessidade da acumulação de carbonizados no leito
do reator para a ocorrência de reações de reforma com vapor. Em segundo, a
mistura de combustíveis derivados de resíduos e biomassa residual florestal
permitiu a melhoria dos produtos de gasificação, constituindo desta forma uma
estratégia bastante promissora a nível económico e ambiental, devido à
elevada abundância e baixo custo dos resíduos urbanos. Contudo, devem ser
efetuadas análises técnico-económicas e de ciclo de vida para a completa
caraterização do processo. Em terceiro, a aplicação de catalisadores de baixo
custo como medida primária demonstrou elevado potencial para a melhoria do
gás produto (e.g., concentração de H2 e CO, poder calorífico inferior) e para o
incremento dos parâmetros de eficiência do processo; em particular, a
aplicação de betão, faialite sintética e carbonizados de pellets de madeira,
demonstrou resultados promissores. Finalmente, foi demonstrada a viabilidade
económica da integração do processo de gasificação direta (ar) de biomassa
na indústria da pasta e papel, apesar dos parâmetros determinados não serem
atrativos para potenciais investidores. Neste contexto, a intervenção dos
governos e o desenvolvimento de instrumentos de apoio económico é de
grande relevância para a implementação destes projetos.Este trabalho foi financiado pela The Navigator Company e por Fundos Nacionais através da Fundação para a Ciência e a Tecnologia (FCT).Programa Doutoral em Engenharia da Refinação, Petroquímica e Químic
Moisture Content and In-place Density of Cold-Recycling Treatments
Cold-recycling treatments are gaining popularity in the United States because of their economic and environmental benefits. Curing is the most critical phase for these treatments. Curing is the process where emulsion breaks and water evaporates, leaving residual binder in the treated material. In this process, the cold-recycled mix gains strength. Sufficient strength is required before opening the cold-treated layer to traffic or placing an overlay. Otherwise, premature failure, related to insufficient strength and trapped moisture, would be expected. However, some challenges arise from the lack of relevant information and specifications to monitor treatment curing. This report presents the outcomes of a research project funded by the Illinois Department for Transportation to investigate the feasibility of using the nondestructive ground-penetrating radar (GPR) for density and moisture content estimation of cold-recycled treatments. Monitoring moisture content is an indicator of curing level; treated layers must meet a threshold of maximum allowable moisture content (2% in Illinois) to be considered sufficiently cured. The methodology followed in this report included GPR numerical simulations and GPR indoor and field tests for data sources. The data were used to correlate moisture content to dielectric properties calculated from GPR measurements. Two models were developed for moisture content estimation: the first is based on numerical simulations and the second is based on electromagnetic mixing theory and called the Al-Qadi-Cao-Abufares (ACA) model. The simulation model had an average error of 0.33% for moisture prediction for five different field projects. The ACA model had an average error of 2% for density prediction and an average root-mean-square error of less than 0.5% for moisture content prediction for both indoor and field tests. The ACA model is presented as part of a developed user-friendly tool that could be used in the future to continuously monitor curing of cold-recycled treatments.IDOT-R27-227Ope
Review of Methodologies to Assess Bridge Safety During and After Floods
This report summarizes a review of technologies used to monitor bridge scour with an emphasis on techniques appropriate for testing during and immediately after design flood conditions. The goal of this study is to identify potential technologies and strategies for Illinois Department of Transportation that may be used to enhance the reliability of bridge safety monitoring during floods from local to state levels. The research team conducted a literature review of technologies that have been explored by state departments of transportation (DOTs) and national agencies as well as state-of-the-art technologies that have not been extensively employed by DOTs. This review included informational interviews with representatives from DOTs and relevant industry organizations. Recommendations include considering (1) acquisition of tethered kneeboard or surf ski-mounted single-beam sonars for rapid deployment by local agencies, (2) acquisition of remote-controlled vessels mounted with single-beam and side-scan sonars for statewide deployment, (3) development of large-scale particle image velocimetry systems using remote-controlled drones for stream velocity and direction measurement during floods, (4) physical modeling to develop Illinois-specific hydrodynamic loading coefficients for Illinois bridges during flood conditions, and (5) development of holistic risk-based bridge assessment tools that incorporate structural, geotechnical, hydraulic, and scour measurements to provide rapid feedback for bridge closure decisions.IDOT-R27-SP50Ope
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