The Phyre2 web portal for protein modeling, prediction and analysis

Phyre2 is a suite of tools available on the web to predict and analyze protein structure, function and mutations. The focus of Phyre2 is to provide biologists with a simple and intuitive interface to state-of-the-art protein bioinformatics tools. Phyre2 replaces Phyre, the original version of the server for which we previously published a paper in Nature Protocols. In this updated protocol, we describe Phyre2, which uses advanced remote homology detection methods to build 3D models, predict ligand binding sites and analyze the effect of amino acid variants (e.g., nonsynonymous SNPs (nsSNPs)) for a user's protein sequence. Users are guided through results by a simple interface at a level of detail they determine. This protocol will guide users from submitting a protein sequence to interpreting the secondary and tertiary structure of their models, their domain composition and model quality. A range of additional available tools is described to find a protein structure in a genome, to submit large number of sequences at once and to automatically run weekly searches for proteins that are difficult to model. The server is available at http://www.sbg.bio.ic.ac.uk/phyre2. A typical structure prediction will be returned between 30 min and 2 h after submission

A Generative Model of People in Clothing

We present the first image-based generative model of people in clothing for the full body. We sidestep the commonly used complex graphics rendering pipeline and the need for high-quality 3D scans of dressed people. Instead, we learn generative models from a large image database. The main challenge is to cope with the high variance in human pose, shape and appearance. For this reason, pure image-based approaches have not been considered so far. We show that this challenge can be overcome by splitting the generating process in two parts. First, we learn to generate a semantic segmentation of the body and clothing. Second, we learn a conditional model on the resulting segments that creates realistic images. The full model is differentiable and can be conditioned on pose, shape or color. The result are samples of people in different clothing items and styles. The proposed model can generate entirely new people with realistic clothing. In several experiments we present encouraging results that suggest an entirely data-driven approach to people generation is possible