4 research outputs found
Automated Correlative Light and Electron Microscopy using FIB-SEM as a tool to screen for ultrastructural phenotypes
In Correlative Light and Electron Microscopy (CLEM), two imaging modalities are combined
to take advantage of the localization capabilities of light microscopy (LM) to guide the capture
of high-resolution details in the electron microscope (EM). However, traditional approaches
have proven to be very laborious, thus yielding a too low throughput for quantitative or
exploratory studies of populations. Recently, in the electron microscopy field, FIB-SEM
(Focused Ion Beam -Scanning Electron Microscope) tomography has emerged as a flexible
method that enables semi-automated 3D volume acquisitions. During my thesis, I developed
CLEMSite, a tool that takes advantage of the semi-automation and scanning capabilities
of the FIB-SEM to automatically acquire volumes of adherent cultured cells.
CLEMSite is a combination of computer vision and machine learning applications with a library for
controlling the microscope ( product from a collaboration with Carl Zeiss GmbH and Fibics Inc.). Thanks to this, the microscope was able to automatically track, find and acquire cell regions previously identified in the light microscope. More specifically, two main modules
were implemented. First, a correlation module was designed to detect and record reference
points from a grid pattern present on the culture substrate in both modalities (LM and EM).
Second, I designed a module that retrieves the regions of interest in the FIB-SEM and that
drives the acquisition of image stacks between different targets in an unattended fashion. The
automated CLEM approach is demonstrated on a project where 3D EM volumes are examined
upon multiple siRNA treatments for knocking down genes involved in the morphogenesis
of the Golgi apparatus. Additionally, the power of CLEM approaches using FIB-SEM is
demonstrated with the detailed structural analysis of two events: the breakage of the nuclear
envelope within constricted cells and an intriguing catastrophic DNA Damage Response in
binucleated cells. Our results demonstrate that executing high throughput volume acquisition
in electron microscopy is possible and that EM can provide incredible insights to guide new
biological discoveries
Collected Papers (on Neutrosophic Theory and Applications), Volume VI
This sixth volume of Collected Papers includes 74 papers comprising 974 pages on (theoretic and applied) neutrosophics, written between 2015-2021 by the author alone or in collaboration with the following 121 co-authors from 19 countries: Mohamed Abdel-Basset, Abdel Nasser H. Zaied, Abduallah Gamal, Amir Abdullah, Firoz Ahmad, Nadeem Ahmad, Ahmad Yusuf Adhami, Ahmed Aboelfetouh, Ahmed Mostafa Khalil, Shariful Alam, W. Alharbi, Ali Hassan, Mumtaz Ali, Amira S. Ashour, Asmaa Atef, Assia Bakali, Ayoub Bahnasse, A. A. Azzam, Willem K.M. Brauers, Bui Cong Cuong, Fausto Cavallaro, Ahmet Çevik, Robby I. Chandra, Kalaivani Chandran, Victor Chang, Chang Su Kim, Jyotir Moy Chatterjee, Victor Christianto, Chunxin Bo, Mihaela Colhon, Shyamal Dalapati, Arindam Dey, Dunqian Cao, Fahad Alsharari, Faruk Karaaslan, Aleksandra Fedajev, Daniela Gîfu, Hina Gulzar, Haitham A. El-Ghareeb, Masooma Raza Hashmi, Hewayda El-Ghawalby, Hoang Viet Long, Le Hoang Son, F. Nirmala Irudayam, Branislav Ivanov, S. Jafari, Jeong Gon Lee, Milena Jevtić, Sudan Jha, Junhui Kim, Ilanthenral Kandasamy, W.B. Vasantha Kandasamy, Darjan Karabašević, Songül Karabatak, Abdullah Kargın, M. Karthika, Ieva Meidute-Kavaliauskiene, Madad Khan, Majid Khan, Manju Khari, Kifayat Ullah, K. Kishore, Kul Hur, Santanu Kumar Patro, Prem Kumar Singh, Raghvendra Kumar, Tapan Kumar Roy, Malayalan Lathamaheswari, Luu Quoc Dat, T. Madhumathi, Tahir Mahmood, Mladjan Maksimovic, Gunasekaran Manogaran, Nivetha Martin, M. Kasi Mayan, Mai Mohamed, Mohamed Talea, Muhammad Akram, Muhammad Gulistan, Raja Muhammad Hashim, Muhammad Riaz, Muhammad Saeed, Rana Muhammad Zulqarnain, Nada A. Nabeeh, Deivanayagampillai Nagarajan, Xenia Negrea, Nguyen Xuan Thao, Jagan M. Obbineni, Angelo de Oliveira, M. Parimala, Gabrijela Popovic, Ishaani Priyadarshini, Yaser Saber, Mehmet Șahin, Said Broumi, A. A. Salama, M. Saleh, Ganeshsree Selvachandran, Dönüș Șengür, Shio Gai Quek, Songtao Shao, Dragiša Stanujkić, Surapati Pramanik, Swathi Sundari Sundaramoorthy, Mirela Teodorescu, Selçuk Topal, Muhammed Turhan, Alptekin Ulutaș, Luige Vlădăreanu, Victor Vlădăreanu, Ştefan Vlăduţescu, Dan Valeriu Voinea, Volkan Duran, Navneet Yadav, Yanhui Guo, Naveed Yaqoob, Yongquan Zhou, Young Bae Jun, Xiaohong Zhang, Xiao Long Xin, Edmundas Kazimieras Zavadskas