Inverter-Based Low-Voltage CCII- Design and Its Filter Application

This paper presents a negative type second-generation current conveyor (CCII-). It is based on an inverter-based low-voltage error amplifier, and a negative current mirror. The CCII- could be operated in a very low supply voltage such as ±0.5V. The proposed CCII- has wide input voltage range (±0.24V), wide output voltage (±0.24V) and wide output current range (±24mA). The proposed CCII- has no on-chip capacitors, so it can be designed with standard CMOS digital processes. Moreover, the architecture of the proposed circuit without cascoded MOSFET transistors is easily designed and suitable for low-voltage operation. The proposed CCII- has been fabricated in TSMC 0.18μm CMOS processes and it occupies 1189.91 x 1178.43μm2 (include PADs). It can also be validated by low voltage CCII filters

Cloud migration patterns: a multi-cloud service architecture perspective

Many organizations migrate their on-premise software systems to the cloud. However, current coarse-grained cloud migration solutions have made a transparent migration of on-premise applications to the cloud a difficult, sometimes trial-and-error based endeavor. This paper suggests a catalogue of fine-grained service-based cloud architecture migration patterns that target multi-cloud settings and are specified with architectural notations. The proposed migration patterns are based on empirical evi-dence from a number of migration projects, best practices for cloud architectures and a systematic literature review of existing research. The pattern catalogue allows an or-ganization to (1) select appropriate architecture migration patterns based on their ob-jectives, (2) compose them to define a migration plan, and (3) extend them based on the identification of new patterns in new contexts

Rift Valley fever virus structural and nonstructural proteins: recombinant protein expression and immunoreactivity against antisera from sheep

The Rift Valley fever virus (RVFV) encodes the structural proteins nucleoprotein (N), aminoterminal glycoprotein (Gn), carboxyterminal glycoprotein (Gc), and L protein, 78-kD, and the nonstructural proteins NSm and NSs. Using the baculovirus system, we expressed the full-length coding sequence of N, NSs, NSm, Gc, and the ectodomain of the coding sequence of the Gn glycoprotein derived from the virulent strain of RVFV ZH548. Western blot analysis using anti-His antibodies and monoclonal antibodies against Gn and N confirmed expression of the recombinant proteins, and in vitro biochemical analysis showed that the two glycoproteins, Gn and Gc, were expressed in glycosylated form. Immunoreactivity profiles of the recombinant proteins in western blot and in indirect enzyme-linked immunosorbent assay against a panel of antisera obtained from vaccinated or wild type (RVFV)-challenged sheep confirmed the results obtained with anti-His antibodies and demonstrated the suitability of the baculo-expressed antigens for diagnostic assays. In addition, these recombinant proteins could be valuable for the development of diagnostic methods that differentiate infected from vaccinated animals (DIVA)

Integral Matrix Gram Root and Lattice Gaussian Sampling without Floats

Many advanced lattice based cryptosystems require to sample lattice points from Gaussian distributions. On

A glycoprotein subunit vaccine elicits a strong Rift Valley fever virus neutralizing antibody response in sheep

Rift Valley fever virus (RVFV), a member of the Bunyaviridae family, is a mosquito-borne zoonotic pathogen that causes serious morbidity and mortality in livestock and humans. The recent spread of the virus beyond its traditional endemic boundaries in Africa to the Arabian Peninsula coupled with the presence of susceptible vectors in non-endemic countries has created increased interest in RVF vaccines. Subunit vaccines composed of specific virus proteins expressed in eukaryotic or prokaryotic expression systems are shown to elicit neutralizing antibodies in susceptible hosts. RVFV structural proteins, N-terminus glycoprotein (Gn) and C-terminus glycoprotein (Gc), were expressed using a recombinant baculovirus expression system. The recombinant proteins were reconstituted as GnGc subunit vaccine formulation and evaluated for immunogenicity in a target species, sheep. Six sheep were each immunized with a primary dose of 50 μg of each vaccine immunogen adjuvanted with montanide ISA25, and at day 21 post-vaccination, each animal received a second dose of the same vaccine. The vaccine induced strong antibody response in all animals as determined by indirect enzyme-linked immunosorbent assay (ELISA). Plaque reduction neutralization test (PRNT80) showed the primary dose of the vaccine was sufficient to elicit potentially protective virus neutralizing antibody titers ranging from 40 to 160, and the second vaccine dose boosted the titer to more than 1,280. Further, all animals tested positive for neutralizing antibodies at day 328 pv. ELISA analysis using the recombinant nucleocapsid protein as a negative marker antigen indicated that the vaccine candidate is DIVA (differentiating infected from vaccinated animals) compatible, and represents a promising vaccine platform for RVFV infection in susceptible species

Pattern-based multi-cloud architecture migration

Many organizations migrate on-premise software applications to the cloud. However, current coarse-grained cloud migration solutions have made such migrations a non transparent task, an endeavor based on trial-anderror. This paper presents Variability-based, Pattern-driven Architecture Migration .V-PAM), a migration method based on (i) a catalogue of fine-grained service-based cloud architecture migration patterns that target multi-cloud, (ii) a situational migration process framework to guide pattern selection and composition, and (iii) a variability model to structure system migration into a coherent framework. The proposed migration patterns are based on empirical evidence from several migration projects, best practice for cloud architectures and a systematic literature review of existing research. Variability-based, Pattern-driven Architecture Migration allows an organization to (i) select appropriate migration patterns, (ii) compose them to define a migration plan, and (iii) extend them based on the identification of new patterns in new contexts. The patterns are at the core of our solution, embedded into a process model, with their selection governed by a variability model

Contributions to Lattice–based Cryptography

Post–quantum cryptography (PQC) is a new and fast–growing part of Cryptography. It focuses on developing cryptographic algorithms and protocols that resist quantum adversaries (i.e., the adversaries who have access to quantum computers). To construct a new PQC primitive, a designer must use a mathematical problem intractable for the quantum adversary. Many intractability assumptions are being used in PQC. There seems to be a consensus in the research community that the most promising are intractable/hard problems in lattices. However, lattice–based cryptography still needs more research to make it more efficient and practical. The thesis contributes toward achieving either the novelty or the practicality of lattice– based cryptographic systems