Development and implementation of image fusion algorithms based on wavelets

Image fusion is a process of blending the complementary as well as the common features of a set of images, to generate a resultant image with superior information content in terms of subjective as well as objective analysis point of view. The objective of this research work is to develop some novel image fusion algorithms and their applications in various fields such as crack detection, multi spectra sensor image fusion, medical image fusion and edge detection of multi-focus images etc. The first part of this research work deals with a novel crack detection technique based on Non-Destructive Testing (NDT) for cracks in walls suppressing the diversity and complexity of wall images. It follows different edge tracking algorithms such as Hyperbolic Tangent (HBT) filtering and canny edge detection algorithm. The second part of this research work deals with a novel edge detection approach for multi-focused images by means of complex wavelets based image fusion. An illumination invariant hyperbolic tangent filter (HBT) is applied followed by an adaptive thresholding to get the real edges. The shift invariance and directionally selective diagonal filtering as well as the ease of implementation of Dual-Tree Complex Wavelet Transform (DT-CWT) ensure robust sub band fusion. It helps in avoiding the ringing artefacts that are more pronounced in Discrete Wavelet Transform (DWT). The fusion using DT-CWT also solves the problem of low contrast and blocking effects. In the third part, an improved DT-CWT based image fusion technique has been developed to compose a resultant image with better perceptual as well as quantitative image quality indices. A bilateral sharpness based weighting scheme has been implemented for the high frequency coefficients taking both gradient and its phase coherence in accoun

Machine Learning And Image Processing For Noise Removal And Robust Edge Detection In The Presence Of Mixed Noise

The central goal of this dissertation is to design and model a smoothing filter based on the random single and mixed noise distribution that would attenuate the effect of noise while preserving edge details. Only then could robust, integrated and resilient edge detection methods be deployed to overcome the ubiquitous presence of random noise in images. Random noise effects are modeled as those that could emanate from impulse noise, Gaussian noise and speckle noise. In the first step, evaluation of methods is performed based on an exhaustive review on the different types of denoising methods which focus on impulse noise, Gaussian noise and their related denoising filters. These include spatial filters (linear, non-linear and a combination of them), transform domain filters, neural network-based filters, numerical-based filters, fuzzy based filters, morphological filters, statistical filters, and supervised learning-based filters. In the second step, switching adaptive median and fixed weighted mean filter (SAMFWMF) which is a combination of linear and non-linear filters, is introduced in order to detect and remove impulse noise. Then, a robust edge detection method is applied which relies on an integrated process including non-maximum suppression, maximum sequence, thresholding and morphological operations. The results are obtained on MRI and natural images. In the third step, a combination of transform domain-based filter which is a combination of dual tree – complex wavelet transform (DT-CWT) and total variation, is introduced in order to detect and remove Gaussian noise as well as mixed Gaussian and Speckle noise. Then, a robust edge detection is applied in order to track the true edges. The results are obtained on medical ultrasound and natural images. In the fourth step, a smoothing filter, which is a feed-forward convolutional network (CNN) is introduced to assume a deep architecture, and supported through a specific learning algorithm, l2 loss function minimization, a regularization method, and batch normalization all integrated in order to detect and remove impulse noise as well as mixed impulse and Gaussian noise. Then, a robust edge detection is applied in order to track the true edges. The results are obtained on natural images for both specific and non-specific noise-level

Wavelet-Based Enhancement Technique for Visibility Improvement of Digital Images

Image enhancement techniques for visibility improvement of color digital images based on wavelet transform domain are investigated in this dissertation research. In this research, a novel, fast and robust wavelet-based dynamic range compression and local contrast enhancement (WDRC) algorithm to improve the visibility of digital images captured under non-uniform lighting conditions has been developed. A wavelet transform is mainly used for dimensionality reduction such that a dynamic range compression with local contrast enhancement algorithm is applied only to the approximation coefficients which are obtained by low-pass filtering and down-sampling the original intensity image. The normalized approximation coefficients are transformed using a hyperbolic sine curve and the contrast enhancement is realized by tuning the magnitude of the each coefficient with respect to surrounding coefficients. The transformed coefficients are then de-normalized to their original range. The detail coefficients are also modified to prevent edge deformation. The inverse wavelet transform is carried out resulting in a lower dynamic range and contrast enhanced intensity image. A color restoration process based on the relationship between spectral bands and the luminance of the original image is applied to convert the enhanced intensity image back to a color image. Although the colors of the enhanced images produced by the proposed algorithm are consistent with the colors of the original image, the proposed algorithm fails to produce color constant results for some pathological scenes that have very strong spectral characteristics in a single band. The linear color restoration process is the main reason for this drawback. Hence, a different approach is required for tackling the color constancy problem. The illuminant is modeled having an effect on the image histogram as a linear shift and adjust the image histogram to discount the illuminant. The WDRC algorithm is then applied with a slight modification, i.e. instead of using a linear color restoration, a non-linear color restoration process employing the spectral context relationships of the original image is applied. The proposed technique solves the color constancy issue and the overall enhancement algorithm provides attractive results improving visibility even for scenes with near-zero visibility conditions. In this research, a new wavelet-based image interpolation technique that can be used for improving the visibility of tiny features in an image is presented. In wavelet domain interpolation techniques, the input image is usually treated as the low-pass filtered subbands of an unknown wavelet-transformed high-resolution (HR) image, and then the unknown high-resolution image is produced by estimating the wavelet coefficients of the high-pass filtered subbands. The same approach is used to obtain an initial estimate of the high-resolution image by zero filling the high-pass filtered subbands. Detail coefficients are estimated via feeding this initial estimate to an undecimated wavelet transform (UWT). Taking an inverse transform after replacing the approximation coefficients of the UWT with initially estimated HR image, results in the final interpolated image. Experimental results of the proposed algorithms proved their superiority over the state-of-the-art enhancement and interpolation techniques

Wavelet-based image and video super-resolution reconstruction.

Super-resolution reconstruction process offers the solution to overcome the high-cost and inherent resolution limitations of current imaging systems. The wavelet transform is a powerful tool for super-resolution reconstruction. This research provides a detailed study of the wavelet-based super-resolution reconstruction process, and wavelet-based resolution enhancement process (with which it is closely associated). It was addressed to handle an explicit need for a robust wavelet-based method that guarantees efficient utilisation of the SR reconstruction problem in the wavelet-domain, which will lead to a consistent solution of this problem and improved performance. This research proposes a novel performance assessment approach to improve the performance of the existing wavelet-based image resolution enhancement techniques. The novel approach is based on identifying the factors that effectively influence on the performance of these techniques, and designing a novel optimal factor analysis (OFA) algorithm. A new wavelet-based image resolution enhancement method, based on discrete wavelet transform and new-edge directed interpolation (DWT-NEDI), and an adaptive thresholding process, has been developed. The DWT-NEDI algorithm aims to correct the geometric errors and remove the noise for degraded satellite images. A robust wavelet-based video super-resolution technique, based on global motion is developed by combining the DWT-NEDI method, with super-resolution reconstruction methods, in order to increase the spatial-resolution and remove the noise and aliasing artefacts. A new video super-resolution framework is designed using an adaptive local motion decomposition and wavelet transform reconstruction (ALMD-WTR). This is to address the challenge of the super-resolution problem for the real-world video sequences containing complex local motions. The results show that OFA approach improves the performance of the selected wavelet-based methods. The DWT-NEDI algorithm outperforms the state-of-the art wavelet-based algorithms. The global motion-based algorithm has the best performance over the super-resolution techniques, namely Keren and structure-adaptive normalised convolution methods. ALMD-WTR framework surpass the state-of-the-art wavelet-based algorithm, namely local motion-based video super-resolution.PhD in Manufacturin

New algorithms for the analysis of live-cell images acquired in phase contrast microscopy

La détection et la caractérisation automatisée des cellules constituent un enjeu important dans de nombreux domaines de recherche tels que la cicatrisation, le développement de l'embryon et des cellules souches, l’immunologie, l’oncologie, l'ingénierie tissulaire et la découverte de nouveaux médicaments. Étudier le comportement cellulaire in vitro par imagerie des cellules vivantes et par le criblage à haut débit implique des milliers d'images et de vastes quantités de données. Des outils d'analyse automatisés reposant sur la vision numérique et les méthodes non-intrusives telles que la microscopie à contraste de phase (PCM) sont nécessaires. Comme les images PCM sont difficiles à analyser en raison du halo lumineux entourant les cellules et de la difficulté à distinguer les cellules individuelles, le but de ce projet était de développer des algorithmes de traitement d'image PCM dans Matlab® afin d’en tirer de l’information reliée à la morphologie cellulaire de manière automatisée. Pour développer ces algorithmes, des séries d’images de myoblastes acquises en PCM ont été générées, en faisant croître les cellules dans un milieu avec sérum bovin (SSM) ou dans un milieu sans sérum (SFM) sur plusieurs passages. La surface recouverte par les cellules a été estimée en utilisant un filtre de plage de valeurs, un seuil et une taille minimale de coupe afin d'examiner la cinétique de croissance cellulaire. Les résultats ont montré que les cellules avaient des taux de croissance similaires pour les deux milieux de culture, mais que celui-ci diminue de façon linéaire avec le nombre de passages. La méthode de transformée par ondelette continue combinée à l’analyse d'image multivariée (UWT-MIA) a été élaborée afin d’estimer la distribution de caractéristiques morphologiques des cellules (axe majeur, axe mineur, orientation et rondeur). Une analyse multivariée réalisée sur l’ensemble de la base de données (environ 1 million d’images PCM) a montré d'une manière quantitative que les myoblastes cultivés dans le milieu SFM étaient plus allongés et plus petits que ceux cultivés dans le milieu SSM. Les algorithmes développés grâce à ce projet pourraient être utilisés sur d'autres phénotypes cellulaires pour des applications de criblage à haut débit et de contrôle de cultures cellulaires.Automated cell detection and characterization is important in many research fields such as wound healing, embryo development, immune system studies, cancer research, parasite spreading, tissue engineering, stem cell research and drug research and testing. Studying in vitro cellular behavior via live-cell imaging and high-throughput screening involves thousands of images and vast amounts of data, and automated analysis tools relying on machine vision methods and non-intrusive methods such as phase contrast microscopy (PCM) are a necessity. However, there are still some challenges to overcome, since PCM images are difficult to analyze because of the bright halo surrounding the cells and blurry cell-cell boundaries when they are touching. The goal of this project was to develop image processing algorithms to analyze PCM images in an automated fashion, capable of processing large datasets of images to extract information related to cellular viability and morphology. To develop these algorithms, a large dataset of myoblasts images acquired in live-cell imaging (in PCM) was created, growing the cells in either a serum-supplemented (SSM) or a serum-free (SFM) medium over several passages. As a result, algorithms capable of computing the cell-covered surface and cellular morphological features were programmed in Matlab®. The cell-covered surface was estimated using a range filter, a threshold and a minimum cut size in order to look at the cellular growth kinetics. Results showed that the cells were growing at similar paces for both media, but their growth rate was decreasing linearly with passage number. The undecimated wavelet transform multivariate image analysis (UWT-MIA) method was developed, and was used to estimate cellular morphological features distributions (major axis, minor axis, orientation and roundness distributions) on a very large PCM image dataset using the Gabor continuous wavelet transform. Multivariate data analysis performed on the whole database (around 1 million PCM images) showed in a quantitative manner that myoblasts grown in SFM were more elongated and smaller than cells grown in SSM. The algorithms developed through this project could be used in the future on other cellular phenotypes for high-throughput screening and cell culture control applications

Development Of A High Performance Mosaicing And Super-Resolution Algorithm

In this dissertation, a high-performance mosaicing and super-resolution algorithm is described. The scale invariant feature transform (SIFT)-based mosaicing algorithm builds an initial mosaic which is iteratively updated by the robust super resolution algorithm to achieve the final high-resolution mosaic. Two different types of datasets are used for testing: high altitude balloon data and unmanned aerial vehicle data. To evaluate our algorithm, five performance metrics are employed: mean square error, peak signal to noise ratio, singular value decomposition, slope of reciprocal singular value curve, and cumulative probability of blur detection. Extensive testing shows that the proposed algorithm is effective in improving the captured aerial data and the performance metrics are accurate in quantifying the evaluation of the algorithm

Statistical analysis and modeling for biomolecular structures

Most of the recent studies on biomolecules address their three dimensional structure since it is closely related to their functions in a biological system. Determination of structure of biomolecules can be done by using various methods, which rely on data from various experimental instruments or on computational approaches to previously obtained data or datasets. Single particle reconstruction using electron microscopic images of macromolecules has proven resource-wise to be useful and affordable for determining their molecular structure in increasing details. The main goal of this thesis is to contribute to the single particle reconstruction methodology, by adding a process of denoising in the analysis of the cryo-electron microscopic images. First, the denoising methods are briefly surveyed and their efficiencies for filtering cryo-electron microscopic images are evaluated. In this thesis, the focus has been set to information theoretic minimum description length (MDL) principle for coding efficiently the essential part of the signal. This approach can also be applied to reduce noise in signals and here it is used to develop a novel denoising method for cryo-electron microscopic images. An existing denoising method has been modified to suit the given problem in single particle reconstruction. In addition, a more general denoising method has been developed, discovering a novel way to find model class by using the MDL principle. This method was then thoroughly tested and compared with co-existing methods in order to evaluate the utility of denoising in single particle reconstruction. A secondary goal in the research for this thesis deals with studying protein oligomerisation, using computational approaches. The focus has been to recognize interacting residues in proteins for oligomerization and to model the interaction site for hantavirus N-protein. In order to unravel the interaction structure, the approach has been to understand the phenomenon of protein folding towards quaternary structure.reviewe

Entropy in Image Analysis II

Image analysis is a fundamental task for any application where extracting information from images is required. The analysis requires highly sophisticated numerical and analytical methods, particularly for those applications in medicine, security, and other fields where the results of the processing consist of data of vital importance. This fact is evident from all the articles composing the Special Issue "Entropy in Image Analysis II", in which the authors used widely tested methods to verify their results. In the process of reading the present volume, the reader will appreciate the richness of their methods and applications, in particular for medical imaging and image security, and a remarkable cross-fertilization among the proposed research areas