6,486 research outputs found
Computational intelligence approaches to robotics, automation, and control [Volume guest editors]
No abstract available
Methods for Analysing Endothelial Cell Shape and Behaviour in Relation to the Focal Nature of Atherosclerosis
The aim of this thesis is to develop automated methods for the analysis of the
spatial patterns, and the functional behaviour of endothelial cells, viewed under
microscopy, with applications to the understanding of atherosclerosis.
Initially, a radial search approach to segmentation was attempted in order to
trace the cell and nuclei boundaries using a maximum likelihood algorithm; it
was found inadequate to detect the weak cell boundaries present in the available
data. A parametric cell shape model was then introduced to fit an equivalent
ellipse to the cell boundary by matching phase-invariant orientation fields of the
image and a candidate cell shape. This approach succeeded on good quality
images, but failed on images with weak cell boundaries. Finally, a support
vector machines based method, relying on a rich set of visual features, and a
small but high quality training dataset, was found to work well on large numbers
of cells even in the presence of strong intensity variations and imaging noise.
Using the segmentation results, several standard shear-stress dependent parameters
of cell morphology were studied, and evidence for similar behaviour
in some cell shape parameters was obtained in in-vivo cells and their nuclei.
Nuclear and cell orientations around immature and mature aortas were broadly
similar, suggesting that the pattern of flow direction near the wall stayed approximately
constant with age. The relation was less strong for the cell and
nuclear length-to-width ratios.
Two novel shape analysis approaches were attempted to find other properties
of cell shape which could be used to annotate or characterise patterns, since a
wide variability in cell and nuclear shapes was observed which did not appear
to fit the standard parameterisations. Although no firm conclusions can yet be
drawn, the work lays the foundation for future studies of cell morphology.
To draw inferences about patterns in the functional response of cells to flow,
which may play a role in the progression of disease, single-cell analysis was performed
using calcium sensitive florescence probes. Calcium transient rates were
found to change with flow, but more importantly, local patterns of synchronisation
in multi-cellular groups were discernable and appear to change with flow.
The patterns suggest a new functional mechanism in flow-mediation of cell-cell
calcium signalling
DART: Distribution Aware Retinal Transform for Event-based Cameras
We introduce a generic visual descriptor, termed as distribution aware
retinal transform (DART), that encodes the structural context using log-polar
grids for event cameras. The DART descriptor is applied to four different
problems, namely object classification, tracking, detection and feature
matching: (1) The DART features are directly employed as local descriptors in a
bag-of-features classification framework and testing is carried out on four
standard event-based object datasets (N-MNIST, MNIST-DVS, CIFAR10-DVS,
NCaltech-101). (2) Extending the classification system, tracking is
demonstrated using two key novelties: (i) For overcoming the low-sample problem
for the one-shot learning of a binary classifier, statistical bootstrapping is
leveraged with online learning; (ii) To achieve tracker robustness, the scale
and rotation equivariance property of the DART descriptors is exploited for the
one-shot learning. (3) To solve the long-term object tracking problem, an
object detector is designed using the principle of cluster majority voting. The
detection scheme is then combined with the tracker to result in a high
intersection-over-union score with augmented ground truth annotations on the
publicly available event camera dataset. (4) Finally, the event context encoded
by DART greatly simplifies the feature correspondence problem, especially for
spatio-temporal slices far apart in time, which has not been explicitly tackled
in the event-based vision domain.Comment: 12 pages, revision submitted to TPAMI in Nov 201
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