Hyperspectral remote sensing of cyanobacterial pigments as indicators for cell populations and toxins in eutrophic lakes

The growth of mass populations of toxin-producing cyanobacteria is a serious concern for the ecological status of inland waterbodies and for human and animal health. In this study we examined the performance of four semi-analytical algorithms for the retrieval of chlorophyll a (Chl a) and phycocyanin (C-PC) from data acquired by the Compact Airborne Spectrographic Imager-2 (CASI-2) and the Airborne Imaging Spectrometer for Applications (AISA) Eagle sensor. The retrieval accuracies of the semi-analytical models were compared to those returned by optimally calibrated empirical band-ratio algorithms. The best-performing algorithm for the retrieval of Chl a was an empirical band-ratio model based on a quadratic function of the ratio of re!ectance at 710 and 670 nm (R2=0.832; RMSE=29.8%). However, this model only provided a marginally better retrieval than the best semi-analytical algorithm. The best-performing model for the retrieval of C-PC was a semi-analytical nested band-ratio model (R2=0.984; RMSE=3.98 mg m−3). The concentrations of C-PC retrieved using the semi-analytical model were correlated with cyanobacterial cell numbers (R2=0.380) and the particulate and total (particulate plus dissolved) pools of microcystins (R2=0.858 and 0.896 respectively). Importantly, both the empirical and semi-analytical algorithms were able to retrieve the concentration of C-PC at cyanobacterial cell concentrations below current warning thresholds for cyanobacteria in waterbodies. This demonstrates the potential of remote sensing to contribute to early-warning detection and monitoring of cyanobacterial blooms for human health protection at regional and global scales

Remote sensing and bio-geo-optical properties of turbid, productive inland waters: a case study of Lake Balaton

Algal blooms plague freshwaters across the globe, as increased nutrient loads lead to eutrophication of inland waters and the presence of potentially harmful cyanobacteria. In this context, remote sensing is a valuable approach to monitor water quality over broad temporal and spatial scales. However, there remain several challenges to the accurate retrieval of water quality parameters, and the research in this thesis investigates these in an optically complex lake (Lake Balaton, Hungary). This study found that bulk and specific inherent optical properties [(S)IOPs] showed significant spatial variability over the trophic gradient in Lake Balaton. The relationships between (S)IOPs and biogeochemical parameters differed from those reported in ocean and coastal waters due to the high proportion of particulate inorganic matter (PIM). Furthermore, wind-driven resuspension of mineral sediments attributed a high proportion of total attenuation to particulate scattering and increased the mean refractive index (n̅p) of the particle assemblage. Phytoplankton pigment concentrations [chlorophyll-a (Chl-a) and phycocyanin (PC)] were also accurately retrieved from a times series of satellite data over Lake Balaton using semi-analytical algorithms. Conincident (S)IOP data allowed for investigation of the errors within these algorithms, indicating overestimation of phytoplankton absorption [aph(665)] and underestimation of the Chl-a specific absorption coefficient [a*ph(665)]. Finally, Chl-a concentrations were accurately retrieved in a multiscale remote sensing study using the Normalized Difference Chlorophyll Index (NDCI), indicating hyperspectral data is not necessary to retrieve accurate pigment concentrations but does capture the subtle heterogeneity of phytoplankton spatial distribution. The results of this thesis provide a positive outlook for the future of inland water remote sensing, particularly in light of contemporary satellite instruments with continued or improved radiometric, spectral, spatial and temporal coverage. Furthermore, the value of coincident (S)IOP data is highlighted and contributes towards the improvement of remote sensing pigment retrieval in optically complex waters

Remote sensing in shallow lake ecology

Shallow lakes are an important ecological and socio-economic resource. However, the impact of human pressures, both at the lake and catchment scale, has precipitated a decline in the ecological status of many shallow lakes, both in the UK, and throughout Europe. There is now, as direct consequence, unprecedented interest in the assessment and monitoring of ecological status and trajectory in shallow lakes, not least in response to the European Union Water Framework Directive (2000/60/EC). In this context, the spatially-resolving and panoramic data provided by remote sensing platforms may be of immense value in the construction of effective and efficient strategies for the assessment and monitoring of ecological status in shallow lakes and, moreover, in providing new, spatially-explicit, insights into the function of these ecosystems and how they respond to change. This thesis examined the use of remote sensing data for the assessment of (i) phytoplankton abundance and species composition and (ii) aquatic vegetation distribution and ecophysiological status in shallow lakes with a view to establishing the credence of such an approach and its value in limnological research and monitoring activities. High resolution in-situ and airborne remote sensing data was collected during a 2-year sampling campaign in the shallow lakes of the Norfolk Broads. It was demonstrated that semi-empirical algorithms could be formulated and used to provide accurate and robust estimations of the concentration of chlorophyll-a, even in these optically-complex waters. It was further shown that it was possible to differentiate and quantify the abundance of cyanobacteria using the biomarker pigment C-phycocyanin. The subsequent calibration of the imagery obtained from the airborne reconnaissance missions permitted the construction of diurnal and seasonal regional-scale time-series of phytoplankton dynamics in the Norfolk Broads. This approach was able to deliver unique spatial insights into the migratory behaviour of a potentially-toxic cyanobacterial bloom. It was further shown that remote sensing can be used to map the distribution of aquatic plants in shallow lakes, importantly including the extent of submerged vegetation, which is central to the assessment of ecological status. This research theme was subsequently extended in an exploration of the use of remote sensing for assessing the ecophysiological response of wetland plants to nutrient enrichment. It was shown that remote sensing metrics could be constructed for the quantification of plant vigour. The extrapolation of these techniques enabled spatial heterogeneity in the ecophysiological response of Phragmites australis to lake nutrient enrichment to be characterised and assisted the formulation of a mechanistic explanation for the variation in reedswamp performance in these shallow lakes. It is therefore argued that the spatially synoptic data provided by remote sensing has much to offer the assessment, monitoring and policing of ecological status in shallow lakes and, in particular, for facilitating the development of pan-European scale lake surveillance capabilities for the Water Framework Directive (2000/60/EC). It is also suggested that remote sensing can make a valuable contribution to furthering ecological understanding and, most significantly, in enabling ecosystem processes and functions to be examined at the lake-scale

The Use of Sentinel-3 Imagery to Monitor Cyanobacterial Blooms

Cyanobacterial harmful algal blooms (CHABs) have been a concern for aquatic systems, especially those used for water supply and recreation. Thus, the monitoring of CHABs is essential for the establishment of water governance policies. Recently, remote sensing has been used as a tool to monitor CHABs worldwide. Remote monitoring of CHABs relies on the optical properties of pigments, especially the phycocyanin (PC) and chlorophyll-a (chl-a). The goal of this study is to evaluate the potential of recent launch the Ocean and Land Color Instrument (OLCI) on-board the Sentinel-3 satellite to identify PC and chl-a. To do this, OLCI images were collected over the Western part of Lake Erie (U.S.A.) during the summer of 2016, 2017, and 2018. When comparing the use of traditional remote sensing algorithms to estimate PC and chl-a, none was able to accurately estimate both pigments. However, when single and band ratios were used to estimate these pigments, stronger correlations were found. These results indicate that spectral band selection should be re-evaluated for the development of new algorithms for OLCI images. Overall, Sentinel 3/OLCI has the potential to be used to identify PC and chl-a. However, algorithm development is needed

Detecting the Spatial Patterns of Blue-green Algae in Harsha Lake using Landsat 8 Imagery

The incidence of harmful algal blooms (HABs) caused by blue-green algae has been increasing in coastal and freshwater ecosystems of the United States in recent years, and has had great influence on ecosystem, economic, and public health. This thesis aims at testing the feasibility of using machine learning methods in comparison to traditional regression models to detect and map the blue-green algae distribution in low-medium biomass waters (Chl-a \u3c approx. 20 μg/L) from a Landsat 8 image with the support of some in situ Chl-a measurements in Harsha Lake, Ohio. Two algorithms were compared: one is the conventional empirical method – Stepwise Multiple Linear Regression – to see if there is a strong linear relationship between measured Chl-a concentrations and the Landsat 8 spectral data in the study area, and the other is one of the most popular machine learning methods–Random Forests. Major findings include: (1) both a conventional linear regression model and a Random Forests model worked well in mapping the extent and biomass of blue-green algae in Harsha Lake on September 21, 2015, but the Random Forests model outperformed the linear regression model; (2) the prediction surface from the Random Forests method illustrated that 89.30% of Harsha Lake’s area had Chl-a values less than 10 µg/L on the sampling date, while only 10.70% of the entire study area had Chl-a concentrations between 10 µg/L and 20 µg/L. Higher Chl-a values (especially for Chl-a larger than 10 µg/L) were mostly distributed in the mouths of rivers or streams, which might be caused by the influx of nutrients from agricultural or urban land use by rivers and streams. The results show the utility of the Random Forests approach based on Landsat 8 imagery in detecting and quantitatively mapping low biomass HABs, which is considered to be a challenging task

Towards high fidelity mapping of global inland water quality using earth observation data

This body of work aims to contribute advancements towards developing globally applicable water quality retrieval models using Earth Observation data for freshwater systems. Eutrophication and increasing prevalence of potentially toxic algal blooms among global inland water bodies have become a major ecological concersn and require direct attention. There is now a growing necessity to develop pragmatic approaches that allow timely and effective extrapolation of local processes, to spatially resolved global products. This study provides one of the first assessments of the state-ofthe-art for trophic status (chlorophyll-a) retrievals for small water bodies using Sentinel-3 Ocean and Land Color Imager (OLCI). Multiple fieldwork campaigns were undertaken for the collection of common aquatic biogeophysical and bio-optical parameters that were used to validate current atmospheric correction and chlorophyll-a retrieval algorithms. The study highlighted the difficulties of obtaining robust retrieval estimates from a coarse spatial resolution sensor from highly variable eutrophic water bodies. Atmospheric correction remains a difficult challenge to operational freshwater monitoring, however, the study further validated previous work confirming applicability of simple, empirically derived retrieval algorithms using top-of-atmosphere data. The apparent scarcity of paired in-situ optical and biogeophysical data for productive inland waters also hinders our capability to develop and validate robust retrieval algorithms. Radiative transfer modeling was used to fill this gap through the development of a novel synthetic dataset of top-of-atmosphere and bottom-of-atmosphere reflectances, which attempts to encompass the immense natural optical variability present in inland waters. Novel aspects of the synthetic dataset include: 1) physics-based, two-layered, size and type specific phytoplankton IOPs for mixed eukaryotic/cyanobacteria 6 assemblages, 2) calculations of mixed assemblage chl-a fluorescence, 3) modeled phycocyanin concentration derived from assemblage based phycocyanin absorption, 4) and paired sensor-specific TOA reflectances which include optically extreme cases and contribution of green vegetation adjacency. The synthetic bottom-of-atmosphere reflectance spectra were compiled into 13 distinct optical water types similar to those discovered using in-situ data. Inspection showed similar relationships and ranges of concentrations and inherent optical properties of natural waters. This dataset was used to calculate typical surviving water-leaving signal at top-of-atmosphere, as well as first order calculations of the signal-to-noise-ratio (SNR) for the various optical water types, a first for productive inland waters, as well as conduct a sensitivity analysis of cyanobacteria detection from top-of-atmosphere. Finally, the synthetic dataset was used to train and test four state-of-the-art machine learning architectures for multi-parameter retrieval and cross-sensor capability. Initial results provide reliable estimates of water quality parameters and inherent optical properties over a highly dynamic range of water types, at various spectral and spatial sensor resolutions. It is hoped the results of this work incrementally improves inland water Earth observation on multiple aspects of the forward and inverse modelling process, and provides an improvement in our capabilities for routine, global monitoring of inland water quality

Cyanobacteria in Inland Waters: Remote Sensing

Remote sensing plays important roles in managing harmful cyanobacterial blooms. Remote sensing algorithms for monitoring cyanobacterial blooms are grouped into empirical, semi-empirical, and semi-analytical methods. In this chapter, 12 of these methods were selected to be reviewed for their performances when applied to in situ measured field reflectance spectra and airborne or satellite sensor collected image spectra. Five empirical PC algorithms based on either band ratio or baseline calculation showed data-dependent performances, empirical band ratios and the baseline can be used to build semi-empirical models such as double three band baseline (DTBB) and four band baseline model (FBBM) showing stronger performance than the three band model (TBM), and the DTBB even performing stronger than the nested band ratio (NBR). As far as three semi-analytical models concern, the NBR and EIIMIW consistently performed well compared to the QAA pc , but care or recalibration should be practiced for applying both EIIMIW and NBR given caring inherent optical property of non-phycocyanin (PC) constituent in the water column. Although neither DTBB nor FBM cannot be evaluated with satellite MEdium Resolution Imaging Spectrometer (MERIS) and Ocean and Land Color Instrument (OLCI) images, they should be tested in future with hyperspectral satellite images acquired by PRISMA, EnMAP, and HyspIRI