Numerical study of interactive motion of dielectrophoretic particles

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BioMEMS to bionanotechnology: state of the art in integrated biochips and future prospects

Biomedical or Biological Micro-Electro-Mechanical- Systems (BioMEMS) have in recent years become increasingly prevalent and have found widespread use in a wide variety of applications such as diagnostics, therapeutics and tissue engineering. This paper reviews the interdisciplinary work performed in our group in recent years to develop micro-integrated devices to characterize biological entities. We present the use of electrical and mechanically based phenomena to perform characterization and various functions needed for integrated biochips. One sub-system takes advantage of the dielectrophoretic effect to sort and concentrate bacterial cells and viruses within a micro-fluidic biochip. Another sub-system measures impedance changes produced by the metabolic activity of bacterial cells to determine their viability. A third sub-system is used to detect the mass of viruses as they bind to micro-mechanical sensors. The last sub-system described has been used to detect the charge on DNA molecules as it translocates through nanopore channels. These devices with an electronic or mechanical signal output can be very useful in producing practical systems for rapid detection and characterization of cells for a wide variety of applications in the food safety and health diagnostics industries. The paper will also briefly discuss future prospects of BioMEMS and its possible impact and on bionanotechnology

A Magnetically and Electrically Powered Hybrid Micromotor in Conductive Solutions: Synergistic Propulsion Effects and Label-Free Cargo Transport and Sensing

Electrically powered micro- and nanomotors are promising tools for in-vitro single-cell analysis. In particular, single cells can be trapped, transported and electroporated by a Janus particle (JP) using an externally applied electric field. However, while dielectrophoretic (DEP)-based cargo manipulation can be achieved at high-solution conductivity, electrical propulsion of these micromotors becomes ineffective at solution conductivities exceeding 0.3mS/cm. Here, we successfully extended JP cargo manipulation and transport capabilities to conductive near-physiological (<6mS/cm) solutions by combining magnetic field-based micromotor propulsion and navigation with DEP-based manipulation of various synthetic and biological cargos. Combination of a rotating magnetic field and electric field resulted in enhanced micromotor mobility and steering control through tuning of the electric field frequency. conditions are necessary. In addition, we demonstrated the micromotors ability of identifying apoptotic cell among viable and necrotic cells based their dielectrophoretic difference, thus, enabling to analyze the apoptotic status in the single cell samples for drug discovery, cell therapeutics and immunotherapy. We also demonstrated the ability to trap and transport live cells towards regions containing doxorubicin-loaded liposomes. This hybrid micromotor approach for label-free trapping, transporting and sensing of selected cells within conductive solutions, opens new opportunities in drug delivery and single cell analysis, where close-to-physiological medi

Review: Electric field driven pumping in microfluidic device

Pumping of fluids with precise control is one of the key components in a microfluidic device. The electric field has been used as one of the most popular and efficient nonmechanical pumping mechanism to transport fluids in microchannels from the very early stage of microfluidic technology development. This review presents fundamental physics and theories of the different microscale phenomena that arise due to the application of an electric field in fluids, which can be applied for pumping of fluids in microdevices. Specific mechanisms considered in this report are electroosmosis, AC electroosmosis, AC electrothermal, induced charge electroosmosis, traveling wave dielectrophoresis, and liquid dielectrophoresis. Each phenomenon is discussed systematically with theoretical rigor and role of relevant key parameters are identified for pumping in microdevices. We specifically discussed the electric field driven body force term for each phenomenon using generalized Maxwell stress tensor as well as simplified effective dipole moment based method. Both experimental and theoretical works by several researchers are highlighted in this article for each electric field driven pumping mechanism. The detailed understanding of these phenomena and relevant key parameters are critical for better utilization, modulation, and selection of appropriate phenomenon for efficient pumping in a specific microfluidic application

Micro- and nano-electrode arrays for electroanalytical sensing

A systematic investigation of the electrochemical behaviour of two sets of microelectrode arrays, fabricated by standard photolithographic and reactive-ion etching techniques, is presented. The first set of microelectrode arrays had a constant relative centre-centre spacing of 10r (where r is the electrode radius). As a value of r was decreased, the cyclic voltammograms recorded from the array became increasingly peak-shaped, due to merging of the diffusion fields of the individual electrodes. Furthermore, it was shown that the peak current densities obtained were largest for the arrays with the smallest individual electrodes, as were the signal-to-noise ratios (SNRs). Electroplating the individuals electrodes with platinum black was also shown to increase the peak currents and the SNRs, due to an increase in the effective surface area. Sigmoidal voltammograms, which are indicative of radial diffusion, were obtained for an individual electrode radius of 25 mm but not for arrays with smaller electrodes. To obtain radial diffusion for an array of 2.5 mm electrodes, it was shown (using a second set of microelectrode arrays) that a minimum relative centre-centre spacing of 40r is required. Further enhancement of the peak current densities were obtained by decreasing the size of the individual electrodes. A series of nanoelectrode arrays were fabricated using electron-beam lithography (EBL). The voltammograms obtained from these arrays exhibited a continual increase in the recorded peak current as the individual electrodes radius was decreased to a value of 110 nm. Since EBL is a slow and costly technique, nanoimprint lithography (NIL) was investigated as an alternative method of fabricating nanoelectrode arrays and comparable results were obtained from arrays produced by EBL and NIL. A dissolved oxygen and temperature sensor incorporating a working microelectrode array was also designed and fabricated. The sector comprised a densely packed array of 2.5 mm radius electrodes and a micro-reference electrode, both of which were covered with an agarose electrolyte gel enclosed in an SU8 chamber. A thermal resistor was included for temperature compensation of the dissolved oxygen measurements. The Ag|AgCl micro-reference electrode was found to be stable for approximately 80 hours in 0.1 M KCl, with 100 nA of current passing through it. Linear calibration curves were obtained from both temperature and dissolved oxygen measurement

Dielectrophoretic nanotweezers for single-particle force spectroscopy

Studying the structural and functional properties of biological molecules using single-molecule techniques has been fundamental in establishing a comprehensive understanding of the mechanisms that govern complex living systems. The aim of this thesis was to develop a dielectrophoretic nanotweezers setup to provide a new approach for applying and measuring dielectrophoretic forces on individual particles in aqueous solution. To achieve this goal, dual-barrel quartz nanopipettes were filled with pyrolytic carbon, forming a pair of nanoelectrodes at their tip. Their size and shape were characterised both through SEM imaging and electrochemical cyclic voltammetry. By applying low AC voltages (< 1 V) between these nanoelectrodes, very strong and highly inhomogeneous electric fields were generated at the nanopipette’s tip to form dielectrophoretic nanotweezers. The frequency of the electric field was tuned so that individual entities were either attracted or repelled depending on their dielectric properties. In this work, experimental measurements of the dielectrophoretic force acting on single polystyrene beads (2 μm) were acquired by single particle tracking on an inverted fluorescence microscope. The spatial coordinates of individual beads were extracted from their trajectories as a function of time under trapping conditions. The force magnitude, measured from their velocity over distance from the nanotweezers tip, was found in the femtonewtons range for a set of applied voltages and frequencies. In addition, the electric field distribution was simulated close to the nanotweezers tip by a finite element model developed for this system. Estimations of the dielectrophoretic force magnitude for different nanotweezers geometries were also performed. Overall, the simple operational mechanism and design of these dielectrophoretic nanotweezers combined with their ability to be controlled in three-dimensions, make them a versatile and promising platform for single-particle manipulation and force probing

Numerical simulation on foam stability and electrokinetic potential during foam injection

Numerical models in petroleum reservoir simulation are valuable tools to visualize the pattern of reservoir fluid flow and to estimate production oil. Modelling enhanced oil recovery (EOR) processes requires a complex mathematical model to integrate multiphase flow and electrokinetic phenomena. There have been limited studies done in combining foam stability and electrokinetics by using simulation tools. This study aims to build and integrate a numerical model of foam progression and electrokinetic behaviour in foam injection process. This model was built using COMSOL Multiphysics 5.3 software to investigate fluid flow profiles in free liquid films stabilised by anionic surfactant, simulate foam progression and electrokinetic measurement with reasonable accuracy. Validation of the numerical model was done using finite element method. Several recent lab and simulation works were compared with this numerical simulation result to fill the gap in this research area. Hence, the relationship between stability of foam and the associated streaming potential signals was obtained. The finding shows that the algorithms suitable for this foam injection case are continuity equation, conservation of species transport, Navier Stokes equations and electric current conservation. Based on the comparison, this simulation study has high level of similarity with previous experimental and simulation works. Therefore, the effectiveness of the EOR in terms of foam stability can be monitored in real time.http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:13160

Strategies for Controlled Placement of Nanoscale Building Blocks

The capability of placing individual nanoscale building blocks on exact substrate locations in a controlled manner is one of the key requirements to realize future electronic, optical, and magnetic devices and sensors that are composed of such blocks. This article reviews some important advances in the strategies for controlled placement of nanoscale building blocks. In particular, we will overview template assisted placement that utilizes physical, molecular, or electrostatic templates, DNA-programmed assembly, placement using dielectrophoresis, approaches for non-close-packed assembly of spherical particles, and recent development of focused placement schemes including electrostatic funneling, focused placement via molecular gradient patterns, electrodynamic focusing of charged aerosols, and others

Electrohydrodynamic Simulations of Capsule Deformation Using a Dual Time-Stepping Lattice Boltzmann Scheme

Capsules are fluid-filled, elastic membranes that serve as a useful model for synthetic and biological membranes. One prominent application of capsules is their use in modeling the response of red blood cells to external forces. These models can be used to study the cell’s material properties and can also assist in the development of diagnostic equipment. In this work we develop a three dimensional model for numerical simulations of red blood cells under the combined influence of hydrodynamic and electrical forces. The red blood cell is modeled as a biconcave-shaped capsule suspended in an ambient fluid domain. Cell deformation occurs due to fluid motion and electrical forces that arise due to differences in the electrical properties between the internal fluid, external fluid, and cell membrane. The electrostatic equations are solved using the immersed interface method. A finite element method is used to compute the membrane’s elastic forces and the membrane’s bending resistance is described by the Helfrich bending energy functional. The membrane forces are coupled to the fluid equations through the immersed boundary method, where the elastic, bending, and electric forces appear as force densities in the Navier-Stokes equations. The fluid equations are solved using a novel dual time-stepping (DTS) lattice Boltzmann method (LBM), which decouples the fluid and capsule discretizations. The computational efficiency of the DTS scheme is studied for capsules in shear flow where it is found that the newly proposed scheme decreases computational time by a factor of 10 when compared to the standard LBM capsule model. The method is then used to study the dynamics of spherical and biconcave capsules in a combined shear flow and DC electric field. For spherical capsules the effect of field strength, shear rate, membrane capacitance, and membrane conductance are studied. For biconcave capsules the effect of the electric field on the tumbling and tank-treading modes of biconcave capsules is discussed