Extending the honey bee venome with the antimicrobial peptide apidaecin and a protein resembling wasp antigen 5

Honey bee venom is a complex mixture of toxic proteins and peptides. In the present study we tried to extend our knowledge of the venom composition using two different approaches. First, worker venom was analysed by liquid chromatography-mass spectrometry and this revealed the antimicrobial peptide apidaecin for the first time in such samples. Its expression in the venom gland was confirmed by reverse transcription PCR and by a peptidomic analysis of the venom apparatus tissue. Second, genome mining revealed a list of proteins with resemblance to known insect allergens or venom toxins, one of which showed homology to proteins of the antigen 5 (Ag5)/Sol i 3 cluster. It was demonstrated that the honey bee Ag5-like gene is expressed by venom gland tissue of winter bees but not of summer bees. Besides this seasonal variation, it shows an interesting spatial expression pattern with additional production in the hypopharyngeal glands, the brains and the midgut. Finally, our immunoblot study revealed that both synthetic apidaecin and the Ag5-like recombinant from bacteria evoke no humoral activity in beekeepers. Also, no IgG4-based cross-reactivity was detected between the honey bee Ag5-like protein and its yellow jacket paralogue Ves v 5

THaW publications

In 2013, the National Science Foundation\u27s Secure and Trustworthy Cyberspace program awarded a Frontier grant to a consortium of four institutions, led by Dartmouth College, to enable trustworthy cybersystems for health and wellness. As of this writing, the Trustworthy Health and Wellness (THaW) project\u27s bibliography includes more than 130 significant publications produced with support from the THaW grant; these publications document the progress made on many fronts by the THaW research team. The collection includes dissertations, theses, journal papers, conference papers, workshop contributions and more. The bibliography is organized as a Zotero library, which provides ready access to citation materials and abstracts and associates each work with a URL where it may be found, cluster (category), several content tags, and a brief annotation summarizing the work\u27s contribution. For more information about THaW, visit thaw.org