2,844 research outputs found
Automated Fourier space region-recognition filtering for off-axis digital holographic microscopy
Automated label-free quantitative imaging of biological samples can greatly
benefit high throughput diseases diagnosis. Digital holographic microscopy
(DHM) is a powerful quantitative label-free imaging tool that retrieves
structural details of cellular samples non-invasively. In off-axis DHM, a
proper spatial filtering window in Fourier space is crucial to the quality of
reconstructed phase image. Here we describe a region-recognition approach that
combines shape recognition with an iterative thresholding to extracts the
optimal shape of frequency components. The region recognition technique offers
fully automated adaptive filtering that can operate with a variety of samples
and imaging conditions. When imaging through optically scattering biological
hydrogel matrix, the technique surpasses previous histogram thresholding
techniques without requiring any manual intervention. Finally, we automate the
extraction of the statistical difference of optical height between malaria
parasite infected and uninfected red blood cells. The method described here
pave way to greater autonomy in automated DHM imaging for imaging live cell in
thick cell cultures
Achieving diffraction-limited resolution in soft-X-ray Fourier-transform holography
The spatial resolution of microscopic images acquired via X-ray Fourier-transform holography is limited by the source size of the reference wave and by the numerical aperture of the detector. We analyze the interplay between both influences and show how they are matched in practice. We further identify, how high spatial frequencies translate to imaging artifacts in holographic reconstructions where mainly the reference beam limits the spatial resolution. As a solution, three methods are introduced based on numerical post-processing of the reconstruction. The methods comprise apodization of the hologram, refocusing via wave propagation, and deconvolution using the transfer function of the imaging system. In particular for the latter two, we demonstrate that image details smaller than the source size of the reference beam can be recovered up to the diffraction limit of the hologram. Our findings motivate the intentional application of a large reference-wave source enhancing the image contrast in applications with low photon numbers such as single-shot experiments at free-electron lasers or imaging at laboratory sources.BMBF, 05K10KTB, Verbundprojekt: FSP 301 - FLASH: Nanoskopische Systeme. Teilprojekt 1.1: Universelle Experimentierkammer für Streuexperimente mit kohärenten Femtosekunden-Röntgenpulsen Multi Purpose Coherent Scattering Chamber for FLASH and XFEL 'MPscatt
Holographic opto-fluidic microscopy.
Over the last decade microfluidics has created a versatile platform that has significantly advanced the ways in which micro-scale organisms and objects are controlled, processed and investigated, by improving the cost, compactness and throughput aspects of analysis. Microfluidics has also expanded into optics to create reconfigurable and flexible optical devices such as reconfigurable lenses, lasers, waveguides, switches, and on-chip microscopes. Here we present a new opto-fluidic microscopy modality, i.e., Holographic Opto-fluidic Microscopy (HOM), based on lensless holographic imaging. This imaging modality complements the miniaturization provided by microfluidics and would allow the integration of microscopy into existing on-chip microfluidic devices with various functionalities. Our imaging modality utilizes partially coherent in-line holography and pixel super-resolution to create high-resolution amplitude and phase images of the objects flowing within micro-fluidic channels, which we demonstrate by imaging C. elegans, Giardia lamblia, and Mulberry pollen. HOM does not involve complicated fabrication processes or precise alignment, nor does it require a highly uniform flow of objects within microfluidic channels
The coronagraphic Modal Wavefront Sensor: a hybrid focal-plane sensor for the high-contrast imaging of circumstellar environments
The raw coronagraphic performance of current high-contrast imaging
instruments is limited by the presence of a quasi-static speckle (QSS)
background, resulting from instrumental non-common path errors (NCPEs). Rapid
development of efficient speckle subtraction techniques in data reduction has
enabled final contrasts of up to 10-6 to be obtained, however it remains
preferable to eliminate the underlying NCPEs at the source. In this work we
introduce the coronagraphic Modal Wavefront Sensor (cMWS), a new wavefront
sensor suitable for real-time NCPE correction. This pupil-plane optic combines
the apodizing phase plate coronagraph with a holographic modal wavefront
sensor, to provide simultaneous coronagraphic imaging and focal-plane wavefront
sensing using the science point spread function. We first characterise the
baseline performance of the cMWS via idealised closed-loop simulations, showing
that the sensor successfully recovers diffraction-limited coronagraph
performance over an effective dynamic range of +/-2.5 radians root-mean-square
(RMS) wavefront error within 2-10 iterations. We then present the results of
initial on-sky testing at the William Herschel Telescope, and demonstrate that
the sensor is able to retrieve injected wavefront aberrations to an accuracy of
10nm RMS under realistic seeing conditions. We also find that the cMWS is
capable of real-time broadband measurement of atmospheric wavefront variance at
a cadence of 50Hz across an uncorrected telescope sub-aperture. When combined
with a suitable closed-loop adaptive optics system, the cMWS holds the
potential to deliver an improvement in raw contrast of up to two orders of
magnitude over the uncorrected QSS floor. Such a sensor would be eminently
suitable for the direct imaging and spectroscopy of exoplanets with both
existing and future instruments, including EPICS and METIS for the E-ELT.Comment: 14 pages, 12 figures: accepted for publication in Astronomy &
Astrophysic
Phase Retrieval via Matrix Completion
This paper develops a novel framework for phase retrieval, a problem which
arises in X-ray crystallography, diffraction imaging, astronomical imaging and
many other applications. Our approach combines multiple structured
illuminations together with ideas from convex programming to recover the phase
from intensity measurements, typically from the modulus of the diffracted wave.
We demonstrate empirically that any complex-valued object can be recovered from
the knowledge of the magnitude of just a few diffracted patterns by solving a
simple convex optimization problem inspired by the recent literature on matrix
completion. More importantly, we also demonstrate that our noise-aware
algorithms are stable in the sense that the reconstruction degrades gracefully
as the signal-to-noise ratio decreases. Finally, we introduce some theory
showing that one can design very simple structured illumination patterns such
that three diffracted figures uniquely determine the phase of the object we
wish to recover
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