4,696 research outputs found
MIMO Detection for High-Order QAM Based on a Gaussian Tree Approximation
This paper proposes a new detection algorithm for MIMO communication systems
employing high order QAM constellations. The factor graph that corresponds to
this problem is very loopy; in fact, it is a complete graph. Hence, a
straightforward application of the Belief Propagation (BP) algorithm yields
very poor results. Our algorithm is based on an optimal tree approximation of
the Gaussian density of the unconstrained linear system. The finite-set
constraint is then applied to obtain a loop-free discrete distribution. It is
shown that even though the approximation is not directly applied to the exact
discrete distribution, applying the BP algorithm to the loop-free factor graph
outperforms current methods in terms of both performance and complexity. The
improved performance of the proposed algorithm is demonstrated on the problem
of MIMO detection
DIET : new developments and recent results
Among existing grid middleware approaches, one simple, powerful, and flexibleapproach consists of using servers available in different administrative domainsthrough the classic client-server or Remote Procedure Call (RPC) paradigm.Network Enabled Servers (NES) implement this model also called GridRPC.Clients submit computation requests to a scheduler whose goal is to find aserver available on the grid. The aim of this paper is to give an overview of anNES middleware developed in the GRAAL team called DIET and to describerecent developments. DIET (Distributed Interactive Engineering Toolbox) is ahierarchical set of components used for the development of applications basedon computational servers on the grid.Parmi les intergiciels de grilles existants, une approche simple, flexible et performante consiste a utiliser des serveurs disponibles dans des domaines administratifs diffĂ©rents Ă travers le paradigme classique de lâappel de procĂ©dure Ă distance (RPC). Les environnements de ce type, connus sous le terme de Network Enabled Servers, implĂ©mentent ce modĂšle appelĂ© GridRPC. Des clientssoumettent des requĂȘtes de calcul Ă un ordonnanceur dont le but consiste Ă trouver un serveur disponible sur la grille.Le but de cet article est de donner un tour dâhorizon dâun intergiciel dĂ©veloppĂ©dans le projet GRAAL appelĂ© DIET 1. DIET (Distributed Interactive Engineering Toolbox) est un ensemble hiĂ©rarchique de composants utilisĂ©s pour ledĂ©veloppement dâapplications basĂ©es sur des serveurs de calcul sur la grille
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Novel copper-containing membrane monooxygenases (CuMMOs) encoded by alkane-utilizing Betaproteobacteria.
Copper-containing membrane monooxygenases (CuMMOs) are encoded by xmoCAB(D) gene clusters and catalyze the oxidation of methane, ammonia, or some short-chain alkanes and alkenes. In a metagenome constructed from an oilsands tailings pond we detected an xmoCABD gene cluster with <59% derived protein sequence identity to genes from known bacteria. Stable isotope probing experiments combined with a specific xmoA qPCR assay demonstrated that the bacteria possessing these genes were incapable of methane assimilation, but did grow on ethane and propane. Single-cell amplified genomes (SAGs) from propane-enriched samples were screened with the specific PCR assay to identify bacteria possessing the target gene cluster. Multiple SAGs of Betaproteobacteria belonging to the genera Rhodoferax and Polaromonas possessed homologues of the metagenomic xmoCABD gene cluster. Unexpectedly, each of these two genera also possessed other xmoCABD paralogs, representing two additional lineages in phylogenetic analyses. Metabolic reconstructions from SAGs predicted that neither bacterium encoded enzymes with the potential to support catabolic methane or ammonia oxidation, but that both were capable of higher n-alkane degradation. The involvement of the encoded CuMMOs in alkane oxidation was further suggested by reverse transcription PCR analyses, which detected elevated transcription of the xmoA genes upon enrichment of water samples with propane as the sole energy source. Enrichments, isotope incorporation studies, genome reconstructions, and gene expression studies therefore all agreed that the unknown xmoCABD operons did not encode methane or ammonia monooxygenases, but rather n-alkane monooxygenases. This study broadens the known diversity of CuMMOs and identifies these enzymes in non-nitrifying Betaproteobacteria
Two intracellular and cell type-specific bacterial symbionts in the placozoan Trichoplax H2
Placozoa is an enigmatic phylum of simple, microscopic, marine metazoans(1,2). Although intracellular bacteria have been found in all members of this phylum, almost nothing is known about their identity, location and interactions with their host(3-6). We used metagenomic and metatranscriptomic sequencing of single host individuals, plus metaproteomic and imaging analyses, to show that the placozoan Trichoplax sp. H2 lives in symbiosis with two intracellular bacteria. One symbiont forms an undescribed genus in the Midichloriaceae (Rickettsiales)(7,8) and has a genomic repertoire similar to that of rickettsial parasites(9,10), but does not seem to express key genes for energy parasitism. Correlative image analyses and three-dimensional electron tomography revealed that this symbiont resides in the rough endoplasmic reticulum of its host's internal fibre cells. The second symbiont belongs to the Margulisbacteria, a phylum without cultured representatives and not known to form intracellular associations(11-13). This symbiont lives in the ventral epithelial cells of Trichoplax, probably metabolizes algal lipids digested by its host and has the capacity to supplement the placozoan's nutrition. Our study shows that one of the simplest animals has evolved highly specific and intimate associations with symbiotic, intracellular bacteria and highlights that symbioses can provide access to otherwise elusive microbial dark matter
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