4,652 research outputs found
Fuzzy-based Propagation of Prior Knowledge to Improve Large-Scale Image Analysis Pipelines
Many automatically analyzable scientific questions are well-posed and offer a
variety of information about the expected outcome a priori. Although often
being neglected, this prior knowledge can be systematically exploited to make
automated analysis operations sensitive to a desired phenomenon or to evaluate
extracted content with respect to this prior knowledge. For instance, the
performance of processing operators can be greatly enhanced by a more focused
detection strategy and the direct information about the ambiguity inherent in
the extracted data. We present a new concept for the estimation and propagation
of uncertainty involved in image analysis operators. This allows using simple
processing operators that are suitable for analyzing large-scale 3D+t
microscopy images without compromising the result quality. On the foundation of
fuzzy set theory, we transform available prior knowledge into a mathematical
representation and extensively use it enhance the result quality of various
processing operators. All presented concepts are illustrated on a typical
bioimage analysis pipeline comprised of seed point detection, segmentation,
multiview fusion and tracking. Furthermore, the functionality of the proposed
approach is validated on a comprehensive simulated 3D+t benchmark data set that
mimics embryonic development and on large-scale light-sheet microscopy data of
a zebrafish embryo. The general concept introduced in this contribution
represents a new approach to efficiently exploit prior knowledge to improve the
result quality of image analysis pipelines. Especially, the automated analysis
of terabyte-scale microscopy data will benefit from sophisticated and efficient
algorithms that enable a quantitative and fast readout. The generality of the
concept, however, makes it also applicable to practically any other field with
processing strategies that are arranged as linear pipelines.Comment: 39 pages, 12 figure
Modeling of evolving textures using granulometries
This chapter describes a statistical approach to classification of dynamic texture images, called parallel evolution functions (PEFs). Traditional classification methods predict texture class membership using comparisons with a finite set of predefined texture classes and identify the closest class. However, where texture images arise from a dynamic texture evolving over time, estimation of a time state in a continuous evolutionary process is required instead. The PEF approach does this using regression modeling techniques to predict time state. It is a flexible approach which may be based on any suitable image features. Many textures are well suited to a morphological analysis and the PEF approach uses image texture features derived from a granulometric analysis of the image. The method is illustrated using both simulated images of Boolean processes and real images of corrosion. The PEF approach has particular advantages for training sets containing limited numbers of observations, which is the case in many real world industrial inspection scenarios and for which other methods can fail or perform badly. [41] G.W. Horgan, Mathematical morphology for analysing soil structure from images, European Journal of Soil Science, vol. 49, pp. 161ā173, 1998. [42] G.W. Horgan, C.A. Reid and C.A. Glasbey, Biological image processing and enhancement, Image Processing and Analysis, A Practical Approach, R. Baldock and J. Graham, eds., Oxford University Press, Oxford, UK, pp. 37ā67, 2000. [43] B.B. Hubbard, The World According to Wavelets: The Story of a Mathematical Technique in the Making, A.K. Peters Ltd., Wellesley, MA, 1995. [44] H. Iversen and T. Lonnestad. An evaluation of stochastic models for analysis and synthesis of gray-scale texture, Pattern Recognition Letters, vol. 15, pp. 575ā585, 1994. [45] A.K. Jain and F. Farrokhnia, Unsupervised texture segmentation using Gabor filters, Pattern Recognition, vol. 24(12), pp. 1167ā1186, 1991. [46] T. Jossang and F. Feder, The fractal characterization of rough surfaces, Physica Scripta, vol. T44, pp. 9ā14, 1992. [47] A.K. Katsaggelos and T. Chun-Jen, Iterative image restoration, Handbook of Image and Video Processing, A. Bovik, ed., Academic Press, London, pp. 208ā209, 2000. [48] M. KĀØoppen, C.H. Nowack and G. RĀØosel, Pareto-morphology for color image processing, Proceedings of SCIA99, 11th Scandinavian Conference on Image Analysis 1, Kangerlussuaq, Greenland, pp. 195ā202, 1999. [49] S. Krishnamachari and R. Chellappa, Multiresolution Gauss-Markov random field models for texture segmentation, IEEE Transactions on Image Processing, vol. 6(2), pp. 251ā267, 1997. [50] T. Kurita and N. Otsu, Texture classification by higher order local autocorrelation features, Proceedings of ACCV93, Asian Conference on Computer Vision, Osaka, pp. 175ā178, 1993. [51] S.T. Kyvelidis, L. Lykouropoulos and N. Kouloumbi, Digital system for detecting, classifying, and fast retrieving corrosion generated defects, Journal of Coatings Technology, vol. 73(915), pp. 67ā73, 2001. [52] Y. Liu, T. Zhao and J. Zhang, Learning multispectral texture features for cervical cancer detection, Proceedings of 2002 IEEE International Symposium on Biomedical Imaging: Macro to Nano, pp. 169ā172, 2002. [53] G. McGunnigle and M.J. Chantler, Modeling deposition of surface texture, Electronics Letters, vol. 37(12), pp. 749ā750, 2001. [54] J. McKenzie, S. Marshall, A.J. Gray and E.R. Dougherty, Morphological texture analysis using the texture evolution function, International Journal of Pattern Recognition and Artificial Intelligence, vol. 17(2), pp. 167ā185, 2003. [55] J. McKenzie, Classification of dynamically evolving textures using evolution functions, Ph.D. Thesis, University of Strathclyde, UK, 2004. [56] S.G. Mallat, Multiresolution approximations and wavelet orthonormal bases of L2(R), Transactions of the American Mathematical Society, vol. 315, pp. 69ā87, 1989. [57] S.G. Mallat, A theory for multiresolution signal decomposition: the wavelet representation, IEEE Transactions on Pattern Analysis and Machine Intelligence, vol. 11, pp. 674ā693, 1989. [58] B.S. Manjunath and W.Y. Ma, Texture features for browsing and retrieval of image data, IEEE Transactions on Pattern Analysis and Machine Intelligence, vol. 18, pp. 837ā842, 1996. [59] B.S. Manjunath, G.M. Haley and W.Y. Ma, Multiband techniques for texture classification and segmentation, Handbook of Image and Video Processing, A. Bovik, ed., Academic Press, London, pp. 367ā381, 2000. [60] G. Matheron, Random Sets and Integral Geometry, Wiley Series in Probability and Mathematical Statistics, John Wiley and Sons, New York, 1975
A New Method of Gridding for Spot Detection in Microarray Images
A Deoxyribonucleic Acid (DNA) microarray is a collection of microscopic DNA spots attached to a solid surface, such as glass, plastic or silicon chip forming an array. The analysis of DNA microarray images allows the identification of gene expressions to draw biological conclusions for applications ranging from genetic profiling to diagnosis of cancer. The DNA microarray image analysis includes three tasks: gridding, segmentation and intensity extraction. The gridding process is usually divided into two main steps: sub-gridding and spot detection. In this paper, a fully automatic approach to detect the location of spots is proposed. Each spot is associated with a gene and contains the pixels that indicate the level of expression of that particular gene. After gridding, the image is segmented using fuzzy c-means clustering algorithm for separation of spots from the background pixels.Ā The result of the experiment shows that the method presented in this paper is accurate and automatic without human intervention and parameter presetting. Keywords: Microarray Image, Mathematical Morphology, Image Processin
Dynamic laser speckle and fuzzy mathematical morphology applied to studies of chemotaxis towards hydrocarbons
The movement of the microorganisms towards a higher concentration of the chemical attractant is called positive chemotaxis and is involved in the efficiency of chemical degradation. Several studies are focused in this field related to genomics, and towards demonstrating chemotactic responses by bacteria, but there is little information related to the activity and morphology of their response. In this work, we use a recently reported dynamic speckle laser method, to process images and to distinguish motile surface patterns per area of colonisation by applying image processing techniques called fuzzy mathematical morphology (FMM). The images of bacterial colonies are usually surfaced, with vague edges and non-homogeneous grey levels. Hence, conventional image processing methods for shape analysis cannot be applied in these cases. In this paper, we propose the application FMM to solve this problem. The approach given was effective to segment, detect and also to describe colonisation patterns
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