Multimedia Forensics

This book is open access. Media forensics has never been more relevant to societal life. Not only media content represents an ever-increasing share of the data traveling on the net and the preferred communications means for most users, it has also become integral part of most innovative applications in the digital information ecosystem that serves various sectors of society, from the entertainment, to journalism, to politics. Undoubtedly, the advances in deep learning and computational imaging contributed significantly to this outcome. The underlying technologies that drive this trend, however, also pose a profound challenge in establishing trust in what we see, hear, and read, and make media content the preferred target of malicious attacks. In this new threat landscape powered by innovative imaging technologies and sophisticated tools, based on autoencoders and generative adversarial networks, this book fills an important gap. It presents a comprehensive review of state-of-the-art forensics capabilities that relate to media attribution, integrity and authenticity verification, and counter forensics. Its content is developed to provide practitioners, researchers, photo and video enthusiasts, and students a holistic view of the field

Machine Learning Models for Educational Platforms

Scaling up education online and onlife is presenting numerous key challenges, such as hardly manageable classes, overwhelming content alternatives, and academic dishonesty while interacting remotely. However, thanks to the wider availability of learning-related data and increasingly higher performance computing, Artificial Intelligence has the potential to turn such challenges into an unparalleled opportunity. One of its sub-fields, namely Machine Learning, is enabling machines to receive data and learn for themselves, without being programmed with rules. Bringing this intelligent support to education at large scale has a number of advantages, such as avoiding manual error-prone tasks and reducing the chance that learners do any misconduct. Planning, collecting, developing, and predicting become essential steps to make it concrete into real-world education. This thesis deals with the design, implementation, and evaluation of Machine Learning models in the context of online educational platforms deployed at large scale. Constructing and assessing the performance of intelligent models is a crucial step towards increasing reliability and convenience of such an educational medium. The contributions result in large data sets and high-performing models that capitalize on Natural Language Processing, Human Behavior Mining, and Machine Perception. The model decisions aim to support stakeholders over the instructional pipeline, specifically on content categorization, content recommendation, learners’ identity verification, and learners’ sentiment analysis. Past research in this field often relied on statistical processes hardly applicable at large scale. Through our studies, we explore opportunities and challenges introduced by Machine Learning for the above goals, a relevant and timely topic in literature. Supported by extensive experiments, our work reveals a clear opportunity in combining human and machine sensing for researchers interested in online education. Our findings illustrate the feasibility of designing and assessing Machine Learning models for categorization, recommendation, authentication, and sentiment prediction in this research area. Our results provide guidelines on model motivation, data collection, model design, and analysis techniques concerning the above applicative scenarios. Researchers can use our findings to improve data collection on educational platforms, to reduce bias in data and models, to increase model effectiveness, and to increase the reliability of their models, among others. We expect that this thesis can support the adoption of Machine Learning models in educational platforms even more, strengthening the role of data as a precious asset. The thesis outputs are publicly available at https://www.mirkomarras.com

[¹⁸F]fluorination of biorelevant arylboronic acid pinacol ester scaffolds synthesized by convergence techniques

Aim: The development of small molecules through convergent multicomponent reactions (MCR) has been boosted during the last decade due to the ability to synthesize, virtually without any side-products, numerous small drug-like molecules with several degrees of structural diversity.(1) The association of positron emission tomography (PET) labeling techniques in line with the “one-pot” development of biologically active compounds has the potential to become relevant not only for the evaluation and characterization of those MCR products through molecular imaging, but also to increase the library of radiotracers available. Therefore, since the [18F]fluorination of arylboronic acid pinacol ester derivatives tolerates electron-poor and electro-rich arenes and various functional groups,(2) the main goal of this research work was to achieve the 18F-radiolabeling of several different molecules synthesized through MCR. Materials and Methods: [18F]Fluorination of boronic acid pinacol esters was first extensively optimized using a benzaldehyde derivative in relation to the ideal amount of Cu(II) catalyst and precursor to be used, as well as the reaction solvent. Radiochemical conversion (RCC) yields were assessed by TLC-SG. The optimized radiolabeling conditions were subsequently applied to several structurally different MCR scaffolds comprising biologically relevant pharmacophores (e.g. β-lactam, morpholine, tetrazole, oxazole) that were synthesized to specifically contain a boronic acid pinacol ester group. Results: Radiolabeling with fluorine-18 was achieved with volumes (800 μl) and activities (≤ 2 GBq) compatible with most radiochemistry techniques and modules. In summary, an increase in the quantities of precursor or Cu(II) catalyst lead to higher conversion yields. An optimal amount of precursor (0.06 mmol) and Cu(OTf)2(py)4 (0.04 mmol) was defined for further reactions, with DMA being a preferential solvent over DMF. RCC yields from 15% to 76%, depending on the scaffold, were reproducibly achieved. Interestingly, it was noticed that the structure of the scaffolds, beyond the arylboronic acid, exerts some influence in the final RCC, with electron-withdrawing groups in the para position apparently enhancing the radiolabeling yield. Conclusion: The developed method with high RCC and reproducibility has the potential to be applied in line with MCR and also has a possibility to be incorporated in a later stage of this convergent “one-pot” synthesis strategy. Further studies are currently ongoing to apply this radiolabeling concept to fluorine-containing approved drugs whose boronic acid pinacol ester precursors can be synthesized through MCR (e.g. atorvastatin)