78 research outputs found
Bio-Based Materials: Contribution to Advancing Circular Economy
This reprint focuses on studies dealing with bio-based materials and its contribution to a circular economy. Research dealing with recycling, waste conversion to bio-based products, the development of bio-based composites, and surface treatments on cellulose fibres have been included in this reprint
XVI Agricultural Science Congress 2023: Transformation of Agri-Food Systems for Achieving Sustainable Development Goals
The XVI Agricultural Science Congress being jointly organized by the National Academy of Agricultural Sciences
(NAAS) and the Indian Council of Agricultural Research (ICAR) during 10-13 October 2023, at hotel Le Meridien,
Kochi, is a mega event echoing the theme “Transformation of Agri-Food Systems for achieving Sustainable
Development Goals”. ICAR-Central Marine Fisheries Research Institute takes great pride in hosting the XVI ASC,
which will be the perfect point of convergence of academicians, researchers, students, farmers, fishers, traders,
entrepreneurs, and other stakeholders involved in agri-production systems that ensure food and nutritional security
for a burgeoning population.
With impeding challenges like growing urbanization, increasing unemployment, growing population, increasing
food demands, degradation of natural resources through human interference, climate change impacts and natural
calamities, the challenges ahead for India to achieve the Sustainable Development Goals (SDGs) set out by the
United Nations are many. The XVI ASC will provide an interface for dissemination of useful information across all
sectors of stakeholders invested in developing India’s agri-food systems, not only to meet the SDGs, but also to
ensure a stable structure on par with agri-food systems around the world.
It is an honour to present this Book of Abstracts which is a compilation of a total of 668 abstracts that convey the
results of R&D programs being done in India. The abstracts have been categorized under 10 major Themes – 1.
Ensuring Food & Nutritional Security: Production, Consumption and Value addition; 2. Climate Action for Sustainable
Agri-Food Systems; 3. Frontier Science and emerging Genetic Technologies: Genome, Breeding, Gene Editing;
4. Livestock-based Transformation of Food Systems; 5. Horticulture-based Transformation of Food Systems; 6.
Aquaculture & Fisheries-based Transformation of Food Systems; 7. Nature-based Solutions for Sustainable AgriFood Systems; 8. Next Generation Technologies: Digital Agriculture, Precision Farming and AI-based Systems; 9.
Policies and Institutions for Transforming Agri-Food Systems; 10. International Partnership for Research, Education
and Development.
This Book of Abstracts sets the stage for the mega event itself, which will see a flow of knowledge emanating
from a zeal to transform and push India’s Agri-Food Systems to perform par excellence and achieve not only the
SDGs of the UN but also to rise as a world leader in the sector. I thank and congratulate all the participants who
have submitted abstracts for this mega event, and I also applaud the team that has strived hard to publish this
Book of Abstracts ahead of the event. I wish all the delegates and participants a very vibrant and memorable
time at the XVI ASC
Uncovering the Mechanisms Underpinning Melanoma Invasiveness at Single Cell Resolution
Metastatic cancer is responsible for 90% of cancer-related deaths, and a lack of effective therapies has seen survival rates remain bleak. Solid cancer cells escape the primary tumour mass and acquire invasive potential through an epithelial to mesenchymal transition (EMT), regulated by a set of master transcription factors (TFs) known as EMT-TFs. EMT-TF activity is in turn controlled by interacting signalling pathways, including the TGFß, Wnt and NF- κB pathways, which become dysregulated in cancer. Once escaped, metastatic cancer cells employ interchangeable modes of migration, transitioning between fibroblast-like mesenchymal migration and amoeboid migration, where cells display a rounded morphology and navigate the extracellular matrix in a protease independent manner. However, the key molecules that orchestrate the switch between mesenchymal and amoeboid migratory modes remain incompletely understood. This thesis describes a novel 3D spheroid invasion assay and single cell isolation technique that provides detailed 3D data on growth and invasion, and allows for the specific isolation of cells of a given phenotype. Via the expression of a photoconvertible fluorescent protein, compact epithelial cells at the edge of a tumour mass, elongated cells in the process of leaving the mass, and rounded amoeboid cells migrating away from the mass were tagged for isolation. Photoconverted cells were then single-cell sorted by flow cytometry and subjected to paired-end Illumina single cell RNA sequencing. 463 differentially expressed genes were identified via DESeq2 and enriched pathways determined by GSEA analysis. Delta opioid receptor and folate transporter SLC19A1 expression were upregulated in amoeboid migration and their functions investigated via pharmacological perturbation, while INKA1 expression was downregulated in amoeboid cells and its function investigated via inducible overexpression. This work describes a novel, adaptable and readily implementable method for the analysis of the earliest phases of cancer cell invasion, and its application to the identification of genes underpinning the invasiveness of malignant melanoma
The Retina in Health and Disease
Vision is the most important sense in higher mammals. The retina is the first step in visual processing and the window to the brain. It is not surprising that problems arising in the retina lead to moderate to severe visual impairments. We offer here a collection of reviews as well as original papers dealing with various aspects of retinal function as well as dysfunction. New approaches in retinal research are described, such as the expression and localization of the endocannabinoid system in the normal retina and the role of cannabinoid receptors that could offer new avenues of research in the development of potential treatments for retinal diseases. Moreover, new insights are offered in advancing knowledge towards the prevention and cure of visual pathologies, mainly AMD, RP, and diabetic retinopathy
Whole-Body Regeneration
This Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing on the primary challenges of this research. Parts Two and Three explore a series of non-vertebrate zoological contexts that provide experimental models for WBR, showing how they can be approached with cellular tools. Parts Four, Five, and Six discuss the future advancements of WBR, reporting about the cutting-edge techniques in genetics and omics used to dissect the underlying mechanisms of WBR, and systems biology approaches to reach a synthetic view of WBR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Whole-Body Regeneration: Methods and Protocols is a valuable resource for scientists and researchers who want to learn more about this important and developing field
Preclinical Evaluation of Lipid-Based Nanosystems
The use of lipid-based nanosystems, including lipid nanoparticles (solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC)), nanoemulsions, and liposomes, among others, is widespread. Several researchers have described the advantages of different applications of these nanosystems. For instance, they can increase the targeting and bioavailability of drugs, improving therapeutic effects. Their use in the cosmetic field is also promising, owing to their moisturizing properties and ability to protect labile cosmetic actives. Thus, it is surprising that only a few lipid-based nanosystems have reached the market. This can be explained by the strict regulatory requirements of medicines and the occurrence of unexpected in vivo failure, which highlights the need to conduct more preclinical studies.Current research is focused on testing the in vitro, ex vivo, and in vivo efficacy of lipid-based nanosystems to predict their clinical performance. However, there is a lack of method validation, which compromises the comparison between different studies.This book brings together the latest research and reviews that report on in vitro, ex vivo, and in vivo preclinical studies using lipid-based nanosystems. Readers can find up-to-date information on the most common experiments performed to predict the clinical behavior of lipid-based nanosystems. A series of 15 research articles and a review are presented, with authors from 15 different countries, which demonstrates the universality of the investigations that have been carried out in this area
Fluorescence Methods for Investigation of Living Cells and Microorganisms
Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms
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