Optimization of Microchannels and Application of Basic Activation Functions of Deep Neural Network for Accuracy Analysis of Microfluidic Parameter Data

The fabrication of microflow channels with high accuracy in terms of the optimization of the proposed designs, minimization of surface roughness, and flow control of microfluidic parameters is challenging when evaluating the performance of microfluidic systems. The use of conventional input devices, such as peristaltic pumps and digital pressure pumps, to evaluate the flow control of such parameters cannot confirm a wide range of data analysis with higher accuracy because of their operational drawbacks. In this study, we optimized the circular and rectangular-shaped microflow channels of a 100 mu m microfluidic chip using a three-dimensional simulation tool, and analyzed concentration profiles of different regions of the microflow channels. Then, we applied a deep learning (DL) algorithm for the dense layers of the rectified linear unit (ReLU), Leaky ReLU, and Swish activation functions to train and test 1600 experimental and interpolation of data samples which obtained from the microfluidic chip. Moreover, using the same DL algorithm, we configured three models for each of these three functions by changing the internal middle layers of these models. As a result, we obtained a total of 9 average accuracy values of ReLU, Leaky ReLU, and Swish functions for a defined threshold value of 6 x 10(-5) using the trial-and-error method. We applied single-to-five-fold cross-validation technique of deep neural network to avoid overfitting and reduce noises from data-set to evaluate better average accuracy of data of microfluidic parameters

Design Optimization of a Pneumatic Soft Robotic Actuator Using Model-Based Optimization and Deep Reinforcement Learning

We present two frameworks for design optimization of a multi-chamber pneumatic-driven soft actuator to optimize its mechanical performance. The design goal is to achieve maximal horizontal motion of the top surface of the actuator with a minimum effect on its vertical motion. The parametric shape and layout of air chambers are optimized individually with the firefly algorithm and a deep reinforcement learning approach using both a model-based formulation and finite element analysis. The presented modeling approach extends the analytical formulations for tapered and thickened cantilever beams connected in a structure with virtual spring elements. The deep reinforcement learning-based approach is combined with both the model- and finite element-based environments to fully explore the design space and for comparison and cross-validation purposes. The two-chamber soft actuator was specifically designed to be integrated as a modular element into a soft robotic pad system used for pressure injury prevention, where local control of planar displacements can be advantageous to mitigate the risk of pressure injuries and blisters by minimizing shear forces at the skin-pad contact. A comparison of the results shows that designs achieved using the deep reinforcement based approach best decouples the horizontal and vertical motions, while producing the necessary displacement for the intended application. The results from optimizations were compared computationally and experimentally to the empirically obtained design in the existing literature to validate the optimized design and methodology

Deep learning with microfluidics for on-chip droplet generation, control, and analysis

Droplet microfluidics has gained widespread attention in recent years due to its advantages of high throughput, high integration, high sensitivity and low power consumption in droplet-based micro-reaction. Meanwhile, with the rapid development of computer technology over the past decade, deep learning architectures have been able to process vast amounts of data from various research fields. Nowadays, interdisciplinarity plays an increasingly important role in modern research, and deep learning has contributed greatly to the advancement of many professions. Consequently, intelligent microfluidics has emerged as the times require, and possesses broad prospects in the development of automated and intelligent devices for integrating the merits of microfluidic technology and artificial intelligence. In this article, we provide a general review of the evolution of intelligent microfluidics and some applications related to deep learning, mainly in droplet generation, control, and analysis. We also present the challenges and emerging opportunities in this field

A Synergistic Framework Leveraging Autoencoders and Generative Adversarial Networks for the Synthesis of Computational Fluid Dynamics Results in Aerofoil Aerodynamics

In the realm of computational fluid dynamics (CFD), accurate prediction of aerodynamic behaviour plays a pivotal role in aerofoil design and optimization. This study proposes a novel approach that synergistically combines autoencoders and Generative Adversarial Networks (GANs) for the purpose of generating CFD results. Our innovative framework harnesses the intrinsic capabilities of autoencoders to encode aerofoil geometries into a compressed and informative 20-length vector representation. Subsequently, a conditional GAN network adeptly translates this vector into precise pressure-distribution plots, accounting for fixed wind velocity, angle of attack, and turbulence level specifications. The training process utilizes a meticulously curated dataset acquired from JavaFoil software, encompassing a comprehensive range of aerofoil geometries. The proposed approach exhibits profound potential in reducing the time and costs associated with aerodynamic prediction, enabling efficient evaluation of aerofoil performance. The findings contribute to the advancement of computational techniques in fluid dynamics and pave the way for enhanced design and optimization processes in aerodynamics.Comment: 9 pages, 11 figure

The Synergy between Organ-on-a-Chip and Artificial Intelligence for the Study of NAFLD: From Basic Science to Clinical Research

Non-alcoholic fatty liver affects about 25% of global adult population. On the long-term, it is associated with extra-hepatic compliances, multiorgan failure, and death. Various invasive and non-invasive methods are employed for its diagnosis such as liver biopsies, CT scan, MRI, and numerous scoring systems. However, the lack of accuracy and reproducibility represents one of the biggest limitations of evaluating the effectiveness of drug candidates in clinical trials. Organ-on-chips (OOC) are emerging as a cost-effective tool to reproduce in vitro the main NAFLD’s pathogenic features for drug screening purposes. Those platforms have reached a high degree of complexity that generate an unprecedented amount of both structured and unstructured data that outpaced our capacity to analyze the results. The addition of artificial intelligence (AI) layer for data analysis and interpretation enables those platforms to reach their full potential. Furthermore, the use of them do not require any ethic and legal regulation. In this review, we discuss the synergy between OOC and AI as one of the most promising ways to unveil potential therapeutic targets as well as the complex mechanism(s) underlying NAFLD

Cheetah:a computational toolkit for cybergenetic control

Abstract Advances in microscopy, microfluidics, and optogenetics enable single-cell monitoring and environmental regulation and offer the means to control cellular phenotypes. The development of such systems is challenging and often results in bespoke setups that hinder reproducibility. To address this, we introduce Cheetah, a flexible computational toolkit that simplifies the integration of real-time microscopy analysis with algorithms for cellular control. Central to the platform is an image segmentation system based on the versatile U-Net convolutional neural network. This is supplemented with functionality to robustly count, characterize, and control cells over time. We demonstrate Cheetah’s core capabilities by analyzing long-term bacterial and mammalian cell growth and by dynamically controlling protein expression in mammalian cells. In all cases, Cheetah’s segmentation accuracy exceeds that of a commonly used thresholding-based method, allowing for more accurate control signals to be generated. Availability of this easy-to-use platform will make control engineering techniques more accessible and offer new ways to probe and manipulate living cells

Contactless acoustic micro/nano manipulation:a paradigm for next generation applications in life sciences

Acoustic actuation techniques offer a promising tool for contactless manipulation of both synthetic and biological micro/nano agents that encompass different length scales. The traditional usage of sound waves has steadily progressed from mid-air manipulation of salt grains to sophisticated techniques that employ nanoparticle flow in microfluidic networks. State-of-the-art in microfabrication and instrumentation have further expanded the outreach of these actuation techniques to autonomous propulsion of micro-agents. In this review article, we provide a universal perspective of the known acoustic micromanipulation technologies in terms of their applications and governing physics. Hereby, we survey these technologies and classify them with regards to passive and active manipulation of agents. These manipulation methods account for both intelligent devices adept at dexterous non-contact handling of micro-agents, and acoustically induced mechanisms for self-propulsion of micro-robots. Moreover, owing to the clinical compliance of ultrasound, we provide future considerations of acoustic manipulation techniques to be fruitfully employed in biological applications that range from label-free drug testing to minimally invasive clinical interventions