Surface and bulk stresses drive morphological changes in fibrous microtissues

Engineered fibrous tissues consisting of cells encapsulated within collagen gels are widely used three-dimensional in vitro models of morphogenesis and wound healing. Although cell-mediated matrix remodeling that occurs within these scaffolds has been extensively studied, less is known about the mesoscale physical principles governing the dynamics of tissue shape. Here, we show both experimentally and by using computer simulations how surface contraction through the development of surface stresses (analogous to surface tension in fluids) coordinates with bulk contraction to drive shape evolution in constrained three-dimensional microtissues. We used microelectromechanical systems technology to generate arrays of fibrous microtissues and robot-assisted microsurgery to perform local incisions and implantation. We introduce a technique based on phototoxic activation of a small molecule to selectively kill cells in a spatially controlled manner. The model simulations, which reproduced the experimentally observed shape changes after surgical and photochemical operations, indicate that fitting of only bulk and surface contractile moduli is sufficient for the prediction of the equilibrium shape of the microtissues. The computational and experimental methods we have developed provide a general framework to study and predict the morphogenic states of contractile fibrous tissues under external loading at multiple length scales.Published versio

Design and Control of a Two-Wheeled Robotic Walker

This thesis presents the design, construction, and control of a two-wheeled inverted pendulum (TWIP) robotic walker prototype for assisting mobility-impaired users with balance and fall prevention. A conceptual model of the robotic walker is developed and used to illustrate the purpose of this study. A linearized mathematical model of the two-wheeled system is derived using Newtonian mechanics. A control strategy consisting of a decoupled LQR controller and three state variable controllers is developed to stabilize the platform and regulate its behavior with robust disturbance rejection performance. Simulation results reveal that the LQR controller is capable of stabilizing the platform and rejecting external disturbances while the state variable controllers simultaneously regulate the system’s position with smooth and minimum jerk control. A prototype for the two-wheeled system is fabricated and assembled followed by the implementation and tuning of the control algorithms responsible for stabilizing the prototype and regulating its position with optimal performance. Several experiments are conducted, confirming the ability of the decoupled LQR controller to robustly balance the platform while the state variable controllers regulate the platform’s position with smooth and minimum jerk control

A 5-DOFs Robot for Posterior Segment Eye Microsurgery

In retinal surgery clinicians access the internal volume of the eyeball through small scale trocar ports, typically 0.65 mm in diameter, to treat vitreoretinal disorders like idiopathic epiretinal membrane and age-related macular holes. The treatment of these conditions involves the removal of thin layers of diseased tissue, namely the epiretinal membrane and the internal limiting membrane. These membranes have an average thickness of only 60 μm and 2 μm respectively making extremely challenging even for expert clinicians to peel without damaging the surrounding tissue. In this work we present a novel Ophthalmic microsurgery Robot (OmSR) designed to operate a standard surgical forceps used in these procedures with micrometric precision, overcoming the limitations of current robotic systems associated with the offsetting of the remote centre of motion of the end effector when accessing the sclera. The design of the proposed system is presented, and its performance evaluated. The results show that the end effector can be controlled with an accuracy of less than 30 μm and the surgical forceps opening and closing positional error is less than 4.3 μm. Trajectory-following experiments and membrane peeling experiments are also presented, showing promising results in both scenarios

非線形弾性要素による内部力補償に基づく無段階変位–力変換機構の創生 ― 微小操作力で強大な磁気力・把持力・制動力・張力を制御可能とするロボット要素 ―

Tohoku University博士（情報科学）thesi