8,650 research outputs found
Long-Molecule Assessment of Ribosomal DNA and RNA
The genes encoding ribosomal RNA and their transcriptional products are essential for life, however, remain poorly understood. Even with the advent of long-range sequencing methodologies, rDNA loci are difficult to study and remain obscure, prompting the consideration of alternative methods to probing this critical region of the genome. The research outlined in this thesis utilises molecular combing, a fibre stretching technique, to isolate DNA molecules measuring more than 5 Mbp in length. The capture of DNA molecules of this size should assist in exploring the architecture of entire rDNA clusters at the single-molecule level. Combining molecular combing with SNP targeting probes, this study aims to distinguish and assess the arrangement of rDNA promoter variants which have been shown to exhibit dramatically different environmental sensitivity. Additionally, through the application of Oxford Nanopore Technologies direct RNA sequencing, the work here has demonstrated the capture of near full-length rRNA primary transcripts, which will allow for assessing post-transcriptional modification across the length of multiple coding subunits within a single molecule, for the first time. Furthermore, an exploration of RNA modification profiles across sample types representative of different developmental stages has been conducted. This study predicts many sites to be differentially modified across these different developmental conditions, several of which are known to be important for, if not crucial in ribosome biogenesis and function. The work outlined in this thesis provides a framework for future studies to conduct long-molecule, genetic, and epitranscriptome profiling of this vital region of the genome, and its dynamic response to a changing environment
Functional Nanomaterials and Polymer Nanocomposites: Current Uses and Potential Applications
This book covers a broad range of subjects, from smart nanoparticles and polymer nanocomposite synthesis and the study of their fundamental properties to the fabrication and characterization of devices and emerging technologies with smart nanoparticles and polymer integration
Digitalization and Development
This book examines the diffusion of digitalization and Industry 4.0 technologies in Malaysia by focusing on the ecosystem critical for its expansion. The chapters examine the digital proliferation in major sectors of agriculture, manufacturing, e-commerce and services, as well as the intermediary organizations essential for the orderly performance of socioeconomic agents.
The book incisively reviews policy instruments critical for the effective and orderly development of the embedding organizations, and the regulatory framework needed to quicken the appropriation of socioeconomic synergies from digitalization and Industry 4.0 technologies. It highlights the importance of collaboration between government, academic and industry partners, as well as makes key recommendations on how to encourage adoption of IR4.0 technologies in the short- and long-term.
This book bridges the concepts and applications of digitalization and Industry 4.0 and will be a must-read for policy makers seeking to quicken the adoption of its technologies
Crystal Structures of Metal Complexes
This reprint contains 11 papers published in a Special Issue of Molecules entitled "Crystal Structures of Metal Complexes". I will be very happy if readers will be interested in the crystal structures of metal complexes
Interpretable and explainable machine learning for ultrasonic defect sizing
Despite its popularity in literature, there are few examples of machine learning (ML) being used for industrial nondestructive evaluation (NDE) applications. A significant barrier is the ‘black box’ nature of most ML algorithms. This paper aims to improve the interpretability and explainability of ML for ultrasonic NDE by presenting a novel dimensionality reduction method: Gaussian feature approximation (GFA). GFA involves fitting a 2D elliptical Gaussian function an ultrasonic image and storing the seven parameters that describe each Gaussian. These seven parameters can then be used as inputs to data analysis methods such as the defect sizing neural network presented in this paper. GFA is applied to ultrasonic defect sizing for inline pipe inspection as an example application. This approach is compared to sizing with the same neural network, and two other dimensionality reduction methods (the parameters of 6 dB drop boxes and principal component analysis), as well as a convolutional neural network applied to raw ultrasonic images. Of the dimensionality reduction methods tested, GFA features produce the closest sizing accuracy to sizing from the raw images, with only a 23% increase in RMSE, despite a 96.5% reduction in the dimensionality of the input data. Implementing ML with GFA is implicitly more interpretable than doing so with principal component analysis or raw images as inputs, and gives significantly more sizing accuracy than 6 dB drop boxes. Shapley additive explanations (SHAP) are used to calculate how each feature contributes to the prediction of an individual defect’s length. Analysis of SHAP values demonstrates that the GFA-based neural network proposed displays many of the same relationships between defect indications and their predicted size as occur in traditional NDE sizing methods
Integrated Optical Fiber Sensor for Simultaneous Monitoring of Temperature, Vibration, and Strain in High Temperature Environment
Important high-temperature parts of an aero-engine, especially the power-related fuel system and rotor system, are directly related to the reliability and service life of the engine. The working environment of these parts is extremely harsh, usually overloaded with high temperature, vibration and strain which are the main factors leading to their failure. Therefore, the simultaneous measurement of high temperature, vibration, and strain is essential to monitor and ensure the safe operation of an aero-engine.
In my thesis work, I have focused on the research and development of two new sensors for fuel and rotor systems of an aero-engine that need to withstand the same high temperature condition, typically at 900 °C or above, but with different requirements for vibration and strain measurement.
Firstly, to meet the demand for high temperature operation, high vibration sensitivity, and high strain resolution in fuel systems, an integrated sensor based on two fiber Bragg gratings in series (Bi-FBG sensor) to simultaneously measure temperature, strain, and vibration is proposed and demonstrated. In this sensor, an L-shaped cantilever is introduced to improve the vibration sensitivity. By converting its free end displacement into a stress effect on the FBG, the sensitivity of the L-shaped cantilever is improved by about 400% compared with that of straight cantilevers. To compensate for the strain sensitivity of FBGs, a spring-beam strain sensitization structure is designed and the sensitivity is increased to 5.44 pm/με by concentrating strain deformation. A novel decoupling method ‘Steps Decoupling and Temperature Compensation (SDTC)’ is proposed to address the interference between temperature, vibration, and strain. A model of sensing characteristics and interference of different parameters is established to achieve accurate signal decoupling. Experimental tests have been performed and demonstrated the good performance of the sensor.
Secondly, a sensor based on cascaded three fiber Fabry-Pérot interferometers in series (Tri-FFPI sensor) for multiparameter measurement is designed and demonstrated for engine rotor systems that require higher vibration frequencies and greater strain measurement requirements. In this sensor, the cascaded-FFPI structure is introduced to ensure high temperature and large strain simultaneous measurement. An FFPI with a cantilever for high vibration frequency measurement is designed with a miniaturized size and its geometric parameters optimization model is established to investigate the influencing factors of sensing characteristics. A cascaded-FFPI preparation method with chemical etching and offset fusion is proposed to maintain the flatness and high reflectivity of FFPIs’ surface, which contributes to the improvement of measurement accuracy. A new high-precision cavity length demodulation method is developed based on vector matching and clustering-competition particle swarm optimization (CCPSO) to improve the demodulation accuracy of cascaded-FFPI cavity lengths. By investigating the correlation relationship between the cascaded-FFPI spectral and multidimensional space, the cavity length demodulation is transformed into a search for the highest correlation value in space, solving the problem that the cavity length demodulation accuracy is limited by the resolution of spectral wavelengths. Different clustering and competition characteristics are designed in CCPSO to reduce the demodulation error by 87.2% compared with the commonly used particle swarm optimization method. Good performance and multiparameter decoupling have been successfully demonstrated in experimental tests
Novel 129Xe Magnetic Resonance Imaging and Spectroscopy Measurements of Pulmonary Gas-Exchange
Gas-exchange is the primary function of the lungs and involves removing carbon dioxide from the body and exchanging it within the alveoli for inhaled oxygen. Several different pulmonary, cardiac and cardiovascular abnormalities have negative effects on pulmonary gas-exchange. Unfortunately, clinical tests do not always pinpoint the problem; sensitive and specific measurements are needed to probe the individual components participating in gas-exchange for a better understanding of pathophysiology, disease progression and response to therapy.
In vivo Xenon-129 gas-exchange magnetic resonance imaging (129Xe gas-exchange MRI) has the potential to overcome these challenges. When participants inhale hyperpolarized 129Xe gas, it has different MR spectral properties as a gas, as it diffuses through the alveolar membrane and as it binds to red-blood-cells. 129Xe MR spectroscopy and imaging provides a way to tease out the different anatomic components of gas-exchange simultaneously and provides spatial information about where abnormalities may occur.
In this thesis, I developed and applied 129Xe MR spectroscopy and imaging to measure gas-exchange in the lungs alongside other clinical and imaging measurements. I measured 129Xe gas-exchange in asymptomatic congenital heart disease and in prospective, controlled studies of long-COVID. I also developed mathematical tools to model 129Xe MR signals during acquisition and reconstruction. The insights gained from my work underscore the potential for 129Xe gas-exchange MRI biomarkers towards a better understanding of cardiopulmonary disease. My work also provides a way to generate a deeper imaging and physiologic understanding of gas-exchange in vivo in healthy participants and patients with chronic lung and heart disease
Study of soft materials, flexible electronics, and machine learning for fully portable and wireless brain-machine interfaces
Over 300,000 individuals in the United States are afflicted with some form of limited motor function from brainstem or spinal-cord related injury resulting in quadriplegia or some form of locked-in syndrome. Conventional brain-machine interfaces used to allow for communication or movement require heavy, rigid components, uncomfortable headgear, excessive numbers of electrodes, and bulky electronics with long wires that result in greater data artifacts and generally inadequate performance. Wireless, wearable electroencephalograms, along with dry non-invasive electrodes can be utilized to allow recording of brain activity on a mobile subject to allow for unrestricted movement. Additionally, multilayer microfabricated flexible circuits, when combined with a soft materials platform allows for imperceptible wearable data acquisition electronics for long term recording. This dissertation aims to introduce new electronics and training paradigms for brain-machine interfaces to provide remedies in the form of communication and movement for these individuals. Here, training is optimized by generating a virtual environment from which a subject can achieve immersion using a VR headset in order to train and familiarize with the system. Advances in hardware and implementation of convolutional neural networks allow for rapid classification and low-latency target control. Integration of materials, mechanics, circuit and electrode design results in an optimized brain-machine interface allowing for rehabilitation and overall improved quality of life.Ph.D
Systemic Circular Economy Solutions for Fiber Reinforced Composites
This open access book provides an overview of the work undertaken within the FiberEUse project, which developed solutions enhancing the profitability of composite recycling and reuse in value-added products, with a cross-sectorial approach. Glass and carbon fiber reinforced polymers, or composites, are increasingly used as structural materials in many manufacturing sectors like transport, constructions and energy due to their better lightweight and corrosion resistance compared to metals. However, composite recycling is still a challenge since no significant added value in the recycling and reprocessing of composites is demonstrated. FiberEUse developed innovative solutions and business models towards sustainable Circular Economy solutions for post-use composite-made products. Three strategies are presented, namely mechanical recycling of short fibers, thermal recycling of long fibers and modular car parts design for sustainable disassembly and remanufacturing. The validation of the FiberEUse approach within eight industrial demonstrators shows the potentials towards new Circular Economy value-chains for composite materials
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