Development of an integrated microspectrometer using arrayed waveguide grating (AWG)

With non-invasive properties and high sensitivities, portable optical biosensors are extremely desirable for point-of-care (POC) applications. Lab-on-a-chip technology such as microfluidics has been treated as an ideal approach to integrate complex sample processing and analysis units with optical detection elements. Spectroscopic sensing (such as fluorescence, Raman and absorption spectroscopy) remains the most highly developed, widely applied, optical technique. However, conventional spectroscopic sensing systems still rely on bulky and expensive dispersive components such as spectrophotometers in a well established laboratory. The work in this thesis is to develop an integrated dispersive component in combination with a microfluidic chip, providing a portable and inexpensive platform for on-chip spectroscopic sensing. In this study, an arrayed waveguide grating (AWG) design developed for telecommunication is re-engineered and utilized to realise a compact, dispersive optical component operating in the visible spectral region. The AWG devices operating in the visible region (λ_c=680 nm) are designed and fabricated with flame hydrolysis deposited (FHD) silica waveguide material. The micro-spectrometer in this proof of concept study has a small (1 cm x 1 cm) footprint and 8 output channels centred on different wavelengths. A series of fabrication issues and challenges are investigated and discussed for the specific AWG device. Subsequently, a sample cuvette is formed by using lithographic technique and dry etching process. Following this, a PDMS chip with microfluidic channels is bonded with the AWG device, leading to an integrated AWG-microfluidic platform. To the best of the author’s knowledge, this is the first work to integrate a visible AWG device and a microfluidic chip towards spectroscopic sensing. The monolithic integrated AWG microspectrometer–microfluidic platform is demonstrated for fluorescence spectroscopic analysis. Signals from the output channels detected on a camera chip can be used to re-create the complete fluorescence spectrum of an analyte. By making fluorescence measurements of (i) mixed quantum dot solutions, (ii) an organic fluorophore (Cy5) and (iii) the propidium iodide (PI)-DNA assay, the results obtained illustrate the unique advantages of the AWG platform for simultaneous, quantitative multiplex detection and its capability to detect small spectroscopic shifts. Although the current system is designed for fluorescence spectroscopic analysis, in principle, it can be implemented for other types of analysis, such as Raman spectroscopy. Fabricated using established semiconductor industry methods, this miniturised platform holds great potential to create a handheld, low cost biosensor with versatile detection capability. Also, the AWG device design is modified with focusing properties that enable localised spectroscopic measurements. Micro-beads based, multiplexed fluorescence detection is performed with the AWG + CCD system and the results have demonstrated capabilities of using the adapted AWG device for localised, multiplexed fluorescence detections, opening up potential applications in the field of cell sorting and single cell analysis. Furthermore, the AWG-microfluidic device is investigated for absorption spectroscopy measurement. As a test system, the pH dependence of the absorption spectra of bromophenol blue is measured to illustrate how an AWG device could be used as a colorimetric pH sensor. Overall, it is believed that the AWG technology holds great potential to realise a compact, integrated spectroscopic biosensor for point-of-care applications

Beyond solid-state lighting: Miniaturization, hybrid integration, and applications og GaN nano- and micro-LEDs

Gallium Nitride (GaN) light-emitting-diode (LED) technology has been the revolution in modern lighting. In the last decade, a huge global market of efficient, long-lasting and ubiquitous white light sources has developed around the inception of the Nobel-price-winning blue GaN LEDs. Today GaN optoelectronics is developing beyond lighting, leading to new and innovative devices, e.g. for micro-displays, being the core technology for future augmented reality and visualization, as well as point light sources for optical excitation in communications, imaging, and sensing. This explosion of applications is driven by two main directions: the ability to produce very small GaN LEDs (microLEDs and nanoLEDs) with high efficiency and across large areas, in combination with the possibility to merge optoelectronic-grade GaN microLEDs with silicon microelectronics in a fully hybrid approach. GaN LED technology today is even spreading into the realm of display technology, which has been occupied by organic LED (OLED) and liquid crystal display (LCD) for decades. In this review, the technological transition towards GaN micro- and nanodevices beyond lighting is discussed including an up-to-date overview on the state of the art

Towards the development of one dimensional zinc oxide nanostructures as biosensor.

The ability to detect biomarkers at a molecular level is crucial to ensure high survival rates for patients with debilitating or life threatening diseases, for example cancer. The limitation associated with existing detection technologies call for more sensitive, selective, faster, cheaper and smaller biosensors for molecular analysis. Recent material advances made in One-dimensional (1-D) ZnO nanostructures hold great promise for fabricating the next generation of biosensors. Due to very high surface- to-volume ratios, they demonstrate high sensitivity to surface charge transfer and changes in the surrounding electrostatic environment, resulting in the significant modification of conductivity upon adsorption of certain molecules. By combining nature's bio-recognition functionalities with the nanostructure's novel electronic properties, an ultra-sensitive and selective biosensor can be developed. The first part of the work compares the electrical behaviour of 1-D ZnO nanostructures grown via hydrothermal and chemical vapour deposition (CVD) techniques, using the scanning conductance microscopy (SCM). For the first time, the polarisability of CVD grown 1-D ZnO nanostructures was observed. By using polarisability as a qualitative measure of the carriers' mobility, CVD nanostructures are shown to exhibit better carrier mobility and thus are more electrically active than hydrothermal ZnO nanorods. Hydrothermal synthesised ZnO nanostructures have higher defect density, generally oxygen vacancies, due to low oxygen concentration in the water and low temperature growth. The oxygen vacancies, known to be deep level traps, are believed to be the reason why the ZnO hydrothermal sample is less 'electrically active'. The successful implementation of biosensors is strongly related to the interface between the biological recognition system and the nanostructure. A surface plasmon resonance technique (Biacore X) is used to identify functional groups that show strong surface binding to ZnO. The respective binding of hexahistidine and zinc finger moieties to ZnO surface was investigated. For the first time, ZnO nanoparticles were discovered to bind directly to nitrilotriacetic acid (NTA), an aminotricarboxylic acid, pre-immobilised on a sensor chip. Subsequently, beta-cyclodextrin (betaCD) was modified with an NTA-like moiety to form a NTA-linked bioreceptor mimic. Analysis results revealed that NTA-like moiety significantly increased the ability of the native cyclodextrin to bind to the surface of ZnO nanoparticles. Following that, polyglutamic acid was shown to be an excellent intermediate between biological molecules (antibody) and ZnO surface. Employing polyglutamic acid as the intermediate linker between antibody and ZnO surface is novel and is recommended for the fabrication of future generation biosensor

Laser structuring of materials for biomedical applications

Laser processing methods have become very appealing for the fabrication of micro/nano structures. To fabricate 3D structures with high resolution andarbitrary complexity, several material deposition processes are in use. By using appropriate moulding techniques, these structures can be fabricated out of a variety of materials such as polymers, ceramics and composites. In this work different lasers have been investigated regarding their suitability for additive and subtractive patterning of small features for biomedical applications. The main focus is on a technique based on two-photon polymerisation of photosensitive materials; this is a nonlinear optical stereo lithography which allows direct-writing of high-resolution three dimensional structures. During the two-photon absorption process, temporal and spatial overlap of photons leads to nonlinear absorption in a highly localized volume. Absorbed photons induce chemical reactions which cause a polymer to form. Due to the quadratic intensity dependence of the process, resolutions of less than 100nm in polymerized structures can potentially be achieved because of the well-defined polymerization threshold. Here, we have emphasised another regime whereby deep structures (~300µm) can be generated in a single pass. This allows rapid fabrication of structures suitable for cell scaffolds where the length scales are small (~10µm) and are required over long ranges (~cm). A Ti: sapphire femtosecond laser at 800nm wavelength with 150fs pulse duration and 1kHz repetition rate was used to determine the two-photon absorption cross section of photoinitiators. This approach was used to initiate two-photon polymerization of resin allowing the fabrication of cell scaffolds suitable for biomedical applications. Diffraction calculations for the imaging optics employed, show that spherical aberration plays a significant role in determining the feature sizes achieved.For subtractive patterning of materials, a femtosecond laser system and an ArF excimer laser have been used. Using ablative techniques keratin films were processed to investigate physical realisation of the commonly used theoretical bricks-and-mortar description of skin. This structure was successfully fabricated and is being used for skin cream research. Also the threshold fluence for ablation of Polyimide Kapton (HN) foils has been measured at oblique angles as an analogue for corneal sculpturing based on beam scanning

NASA Tech Briefs, May 2008

Topics covered inclde: Deployable Wireless Camera Penetrators; Hand-Held Units for Short-Range Wireless Biotelemetry; Wearable Wireless Telemetry System for Implantable BioMEMS Sensors; Electronic Escape Trails for Firefighters; Architecture for a High-to-Medium-Voltage Power Converter; 24-Way Radial Power Combiner/Divider for 31 to 36 GHz; Three-Stage InP Submillimeter-Wave MMIC Amplifier; Fast Electromechanical Switches Based on Carbon Nanotubes; Solid-State High-Temperature Power Cells; Fast Offset Laser Phase-Locking System; Fabricating High-Resolution X-Ray Collimators; Embossed Teflon AF Laminate Membrane Microfluidic Diaphragm Valves; Flipperons for Improved Aerodynamic Performance; System Estimates Radius of Curvature of a Segmented Mirror; Refractory Ceramic Foams for Novel Applications; Self-Deploying Trusses Containing Shape-Memory Polymers; Fuel-Cell Electrolytes Based on Organosilica Hybrid Proton Conductors; Molecules for Fluorescence Detection of Specific Chemicals; Cell-Detection Technique for Automated Patch Clamping; Redesigned Human Metabolic Simulator; Compact, Highly Stable Ion Atomic Clock; LiGa(OTf)(sub 4) as an Electrolyte Salt for Li-Ion Cells; Compact Dielectric-Rod White-Light Delay Lines; Single-Mode WGM Resonators Fabricated by Diamond Turning; Mitigating Photon Jitter in Optical PPM Communication; MACOS Version 3.31; Fiber-Optic Determination of N2, O2, and Fuel Vapor in the Ullage of Liquid-Fuel Tanks; Spiking Neurons for Analysis of Patterns; Symmetric Phase-Only Filtering in Particle-Image Velocimetry; Efficient Coupler for a Bessel Beam Dispersive Element; and Attitude and Translation Control of a Solar Sail Vehicle

NASA Tech Briefs Index, 1977, volume 2, numbers 1-4

Announcements of new technology derived from the research and development activities of NASA are presented. Abstracts, and indexes for subject, personal author, originating center, and Tech Brief number are presented for 1977

A nanostructured porous silicon based drug delivery device

Targeted and controlled delivery of therapeutic agents on demand is pivotal in realising the efficacy of many pharmaceuticals. The design and fabrication of a novel, electrically-addressable, porous structure-based drug delivery device for the controlled release of therapeutic proteins and peptides, are described in this thesis. The initial prototype microdevice design incorporates a porous polysilicon (PPSi) structure as a drug reservoir. Two alternative methods were investigated to fabricate the PPSi structure: i) the chemical stain etching method; ii) a reactive ion etching (RIE) method through a masking template. Random pores, with irregular pore shape and size in the micro- to mesoporous regime (< 50 nm), were obtained using the stain etching method but this method suffered from poor reproducibility and non-uniformity. Two novel RIE approaches were investigated to fabricate ordered PPSi structures; two different masking templates were investigated – a porous anodic alumina (PAA) and a metal mask with hexagonally arranged holes produced by a novel nanosphere lithography (NSL) technique. A quasi-ordered PAA template with pore diameters in the region of 50 nm was fabricated but was not suitable for the subsequent proposed RIE process. By using the NSL technique, quasi-ordered PPSi structures with tapered pore profiles, were obtained. This is the first demonstration of the fabrication of PPSi with ordered pores of sizes in the macropore range of ~ 370 nm.A revised silicon-based prototype microdevice was designed and fabricated. The microdevice incorporates a nanostructured, quasi-ordered porous silicon (PSi) as a drug reservoir and an integrated heater and temperature sensor as an active control mechanism. The PSi structure was fabricated using a modified NSL technique and a Bosch-based RIE process. Hexagonally arranged cylindrical pores with diameters between ~75 nm and ~120 nm, and depths in the range of ~330 nm and 500 nm, were obtained. The novel fabrication techniques investigated here are simple and versatile; both p-type and n-type PSi structures have been successfully fabricated. Proof-of-concept studies, using the revised prototype drug delivery microdevices, suggested that the nanostructured PSi would be suitable for the passive release of an intermediate-sized (~23,000 Dalton) model protein. It is envisaged that the microdevice has the potential to deliver osteoinductive growth factors, on demand, to the site of fracture, in a controlled and sustainable manner, as a first step to an intelligent therapeutic system for skeletal regeneration

Patterning soft matter for cell culturing

In the search to understand the interaction between cells and their underlying substrates, life sciences are beginning to incorporate micro and nano-technology based tools to probe, measure and improve cellular behavior. In this frame, patterned surfaces provide a platform for highly defined cellular interactions and, in perspective, they offer unique advantages for artificial implants. For these reasons, functionalized materials have recently become a central topic in tissue engineering. Nanotechnology, with its rich toolbox of techniques, can be the leading actor in the materials patterning field. Laser assisted methods, conventional and un-conventional lithography and other patterning techniques, allow the fabrication of functional supports with tunable properties, either physically, or topographically and chemically. Among them, soft lithography provides an effective (and low cost) strategy for manufacturing micro and nanostructures. The main focus of this work is the use of different fabrication approaches aiming at a precise control of cell behavior, adhesion, proliferation and differentiation, through chemically and spatially designed surfaces