Event Detection by Feature Unpredictability in Phase-Contrast Videos of Cell Cultures

Abstract. In this work we propose a novel framework for generic event monitoring in live cell culture videos, built on the assumption that un-predictable observations should correspond to biological events. We use a small set of event-free data to train a multioutput multikernel Gaussian process model that operates as an event predictor by performing autore-gression on a bank of heterogeneous features extracted from consecutive frames of a video sequence. We show that the prediction error of this model can be used as a probability measure of the presence of relevant events, that can enable users to perform further analysis or monitoring of large-scale non-annotated data. We validate our approach in two phase-contrast sequence data sets containing mitosis and apoptosis events: a new private dataset of human bone cancer (osteosarcoma) cells and a benchmark dataset of stem cells

Uropathogenic Escherichia coli P and Type 1 Fimbriae Act in Synergy in a Living Host to Facilitate Renal Colonization Leading to Nephron Obstruction

The progression of a natural bacterial infection is a dynamic process influenced by the physiological characteristics of the target organ. Recent developments in live animal imaging allow for the study of the dynamic microbe-host interplay in real-time as the infection progresses within an organ of a live host. Here we used multiphoton microscopy-based live animal imaging, combined with advanced surgical procedures, to investigate the role of uropathogenic Escherichia coli (UPEC) attachment organelles P and Type 1 fimbriae in renal bacterial infection. A GFP+ expressing variant of UPEC strain CFT073 and genetically well-defined isogenic mutants were microinfused into rat glomerulus or proximal tubules. Within 2 h bacteria colonized along the flat squamous epithelium of the Bowman's capsule despite being exposed to the primary filtrate. When facing the challenge of the filtrate flow in the proximal tubule, the P and Type 1 fimbriae appeared to act in synergy to promote colonization. P fimbriae enhanced early colonization of the tubular epithelium, while Type 1 fimbriae mediated colonization of the center of the tubule via a mechanism believed to involve inter-bacterial binding and biofilm formation. The heterogeneous bacterial community within the tubule subsequently affected renal filtration leading to total obstruction of the nephron within 8 h. Our results reveal the importance of physiological factors such as filtration in determining bacterial colonization patterns, and demonstrate that the spatial resolution of an infectious niche can be as small as the center, or periphery, of a tubule lumen. Furthermore, our data show how secondary physiological injuries such as obstruction contribute to the full pathophysiology of pyelonephritis

Information Technologies and Social Media: New Scientific Methods for the Anthropocene

The development of technology during the Anthropocene has affected science and the ways of “doing science”. Nowadays, new technologies help scientists of several disciplines by facilitating knowledge and how to manage it, but also allow for collaborative science, the so-called “Social Science”, where everyone can be a scientist and be involved in providing data and knowledge by using a computer or a smartphone without being a specialist. But is it really that simple? Actually, the daily and integrated use of different digital technologies and sharing platforms, such as social media, requires important reflections. Such reflections can lead to a rethinking of epistemologies and scientific paradigms, both in human geography and social sciences. This volume titled “Information Technologies and Social Media: New Scientific Methods for the Anthropocene” includes 10 chapters exploring some changes related to the way to do science with a multidisciplinary approach. From classroom experiences to the use of Citizen Science, from Artificial Intelligence use to how Social Media can help researchers, the book reflects on the ICT influence during the last few decades, exploring different cases, complementary perspectives and point of views

Enhancing fan experience during live sports broadcasts through second screen applications

When sports fans attend live sports events, they usually engage in social experiences with friends, family members and other fans at the venue sharing the same affiliation. However, fans watching the same event through a live television broadcast end up not feeling so emotionally connected with the athletes and other fans as they would if they were watching it live, together with thousands of other fans. With this in mind, we seek to create mobile applications that deliver engaging social experiences involving remote fans watching live broadcasted sports events. Taking into account the growing use of mobile devices when watching TV broadcasts, these mobile applications explore the second screen concept, which allows users to interact with content that complements the TV broadcast. Within this context, we present a set of second screen application prototypes developed to test our concepts, the corresponding user studies and results, as well as suggestions on how to apply the prototypes’ concepts not only in different sports, but also during TV shows and electronic sports. Finally, we also present the challenges we faced and the guidelines we followed during the development and evaluation phases, which may give a considerable contribution to the development of future second screen applications for live broadcasted events

Cross-cultural effects on drivers' hazard perception : validating a test paradigm for developing countries

The hazard perception skill of a driver refers to their ability to identify potentially dangerous events on the road, and is one of the only driving-specific skills that has been consistently linked to accident rates. Hazard perception tests are used in several developed countries as part of the driver licensing curriculum, however little research has been done in developing countries where road safety is a primary concern. The extent to which hazard perception skill transfers to different driving environments is also unclear. This thesis therefore has two major aims: to examine hazard perception in a cross-cultural context, and to validate a hazard perception test for potential use in driver licensing in lower-income, developing countries. Most of the experiments in this thesis compare hazard perception skill in drivers from the UK – where hazard perception testing is well established – and drivers from Malaysia – a developing country with a high accident rate where hazards frequently occur. Typically, hazard perception skill is assessed by showing participants clips filmed on the road and asking them to respond as soon as they detect a developing hazard, with shorter response times reflecting greater levels of skill. Chapter 2 presents evidence that Malaysian drivers may be desensitized to hazardous road situations and thus have increased response times to hazards, creating validity issues with the typical paradigm. Subsequent chapters therefore use a predictive paradigm called the “What Happens Next?” test that requires drivers to predict hazards, leaving performance unaffected by hazard desensitization. Malaysian drivers predicted hazards less accurately than UK drivers in all cross-cultural experiments, indicating that exposure to a greater number of hazards on Malaysian roads did not have a positive effect on participants’ predictive hazard perception skill. Further experiments indicated that explicit knowledge plays a minor role in the “What Happens Next?” test, and that experienced drivers appear to compensate for reduced visual information more effectively than novices. Experienced drivers from both Malaysia and the UK also outscored novices in all experiments using the predictive paradigm, suggesting the “What Happens Next?” test provides a valid measure of hazard perception skill and may offer a practical alternative for hazard perception testing in developing and even developed countries

Fish4Knowledge: Collecting and Analyzing Massive Coral Reef Fish Video Data

This book gives a start-to-finish overview of the whole Fish4Knowledge project, in 18 short chapters, each describing one aspect of the project. The Fish4Knowledge project explored the possibilities of big video data, in this case from undersea video. Recording and analyzing 90 thousand hours of video from ten camera locations, the project gives a 3 year view of fish abundance in several tropical coral reefs off the coast of Taiwan. The research system built a remote recording network, over 100 Tb of storage, supercomputer processing, video target detection and

Proceedings of the 1st International Conference on Live Coding

Open Access peer reviewed papers on live coding published at the 1st International Conference on Live Coding (ICLC) in Leeds