REVIEW ON DETECTION OF RICE PLANT LEAVES DISEASES USING DATA AUGMENTATION AND TRANSFER LEARNING TECHNIQUES

The most important cereal crop in the world is rice (Oryza sativa). Over half of the world's population uses it as a staple food and energy source. Abiotic and biotic factors such as precipitation, soil fertility, temperature, pests, bacteria, and viruses, among others, impact the yield production and quality of rice grain. Farmers spend a lot of time and money managing diseases, and they do so using a bankrupt "eye" method that leads to unsanitary farming practices. The development of agricultural technology is greatly conducive to the automatic detection of pathogenic organisms in the leaves of rice plants. Several deep learning algorithms are discussed, and processors for computer vision problems such as image classification, object segmentation, and image analysis are discussed. The paper showed many methods for detecting, characterizing, estimating, and using diseases in a range of crops. The methods of increasing the number of images in the data set were shown. Two methods were presented, the first is traditional reinforcement methods, and the second is generative adversarial networks. And many of the advantages have been demonstrated in the research paper for the work that has been done in the field of deep learning

Quantifying soybean phenotypes using UAV imagery and machine learning, deep learning methods

Crop breeding programs aim to introduce new cultivars to the world with improved traits to solve the food crisis. Food production should need to be twice of current growth rate to feed the increasing number of people by 2050. Soybean is one the major grain in the world and only US contributes around 35 percent of world soybean production. To increase soybean production, breeders still rely on conventional breeding strategy, which is mainly a 'trial and error' process. These constraints limit the expected progress of the crop breeding program. The goal was to quantify the soybean phenotypes of plant lodging and pubescence color using UAV-based imagery and advanced machine learning. Plant lodging and soybean pubescence color are two of the most important phenotypes for soybean breeding programs. Soybean lodging and pubescence color is conventionally evaluated visually by breeders, which is time-consuming and subjective to human errors. The goal of this study was to investigate the potential of unmanned aerial vehicle (UAV)-based imagery and machine learning in the assessment of lodging conditions and deep learning in the assessment pubescence color of soybean breeding lines. A UAV imaging system equipped with an RGB (red-green-blue) camera was used to collect the imagery data of 1,266 four-row plots in a soybean breeding field at the reproductive stage. Soybean lodging scores and pubescence scores were visually assessed by experienced breeders. Lodging scores were grouped into four classes, i.e., non-lodging, moderate lodging, high lodging, and severe lodging. In contrast, pubescence color scores were grouped into three classes, i.e., gray, tawny, and segregation. UAV images were stitched to build orthomosaics, and soybean plots were segmented using a grid method. Twelve image features were extracted from the collected images to assess the lodging scores of each breeding line. Four models, i.e., extreme gradient boosting (XGBoost), random forest (RF), K-nearest neighbor (KNN), and artificial neural network (ANN), were evaluated to classify soybean lodging classes. Five data pre-processing methods were used to treat the imbalanced dataset to improve the classification accuracy. Results indicate that the pre-processing method SMOTE-ENN consistently performs well for all four (XGBoost, RF, KNN, and ANN) classifiers, achieving the highest overall accuracy (OA), lowest misclassification, higher F1-score, and higher Kappa coefficient. This suggests that Synthetic Minority Over-sampling-Edited Nearest Neighbor (SMOTE-ENN) may be an excellent pre-processing method for using unbalanced datasets and classification tasks. Furthermore, an overall accuracy of 96 percent was obtained using the SMOTE-ENN dataset and ANN classifier. On the other hand, to classify the soybean pubescence color, seven pre-trained deep learning models, i.e., DenseNet121, DenseNet169, DenseNet201, ResNet50, InceptionResNet-V2, Inception-V3, and EfficientNet were used, and images of each plot were fed into the model. Data was enhanced using two rotational and two scaling factors to increase the datasets. Among the seven pre-trained deep learning models, ResNet50 and DenseNet121 classifiers showed a higher overall accuracy of 88 percent, along with higher precision, recall, and F1-score for all three classes of pubescence color. In conclusion, the developed UAV-based high-throughput phenotyping system can gather image features to estimate soybean crucial phenotypes and classify the phenotypes, which will help the breeders in phenotypic variations in breeding trials. Also, the RGB imagery-based classification could be a cost-effective choice for breeders and associated researchers for plant breeding programs in identifying superior genotypes.Includes bibliographical references

Kansas Field Research 2023

A summary of research conducted in 2021-2023 on field production and management practices for crops in Kansas. Published in 2023 from the Kansas State University Agricultural Experiment Station and Cooperative Extension Service. https://www.ag.k-state.edu

Combining remote sensing and crop modeling techniques to derive a nitrogen fertilizer application strategy

The crucial question in this thesis was how can remote sensing data and crop models be used to derive a N fertilizer strategy that is capable to lower the environmental side effects of N fertilizer application. This raised the following detailed objectives: The first objective (i) how N content determination via spectral reflectance is influenced by different leaves and positions on the leaf was investigated in Publication I. Different wheat plants were cultivated under different N levels and under drought stress in two hydroponic greenhouse trials. Spectral reflectance measurements were taken from three leaves and at three positions on the leaf for each plant. In total, 16 vegetation indices broadly used in the literature were calculated based on the spectral reflectance for each combination of leaf and position. The plant N content was determined by lab analyses. Neither the position on the leaf nor leaf number had an impact on the accuracy of plant N determination via spectral reflectance measurements. Therefore measurements taken at the canopy level seem to be a valid approach. However, if other stress symptoms like drought or disease infection occur, a differentiation between leaves and positions on the leaf might play a more crucial role. Publication II dealt with the second objective on (ii), how to incorporate leaf disease into the DSSAT wheat model to enable the simulation of the impact of leaf disease on yield. An integration of sensor information in crop growth models requires the update of model state variables. A model extension was developed by adding a pest damage module to the existing wheat model. The approach was tested on a two-year dataset from Argentina with different wheat cultivars and on a one-year dataset from Germany with different inoculum levels of septoria tritici blotch (STB). After the integration of disease infection, the accuracy of the simulated yield and leaf area index (LAI) was improved. The Root mean squared error (RMSE) values for yield (1144 kg ha−1) and LAI (1.19 m2 m−2) were reduced by half (499 kg ha−1) for yield and LAI (0.69 m2 m−2). A sensitivity analysis also showed a strong responsiveness of the model by the integration of different STB disease infection scenarios. Increasing the modeling accuracy even further a MM approach seems to be suitable. Assembling more models increases the complexity of the simulation and the involved calibration procedure especially if the user is not familiar with all models. To avoid these conflicts, Publication III evaluated the third objective (iii) if an automatic calibration procedure in a MM approach for winter wheat can eliminate the subjectivity factor in model calibration. The model calibration was performed on a 4-yr N wheat fertilizer trial in southwest Germany. The evaluation mean showed satisfying results for the calibration (d-Index 0.93) and evaluation dataset (d-Index 0.81). This lead to the fourth (iv) objective to use a MM approach to improve the overall modeling accuracy. The evaluation of a fertilizer trial showed an improved modeling accuracy in most cases, especially in the drought season 2018. Based on the combination of a MM approach and the incorporation of sensor data, a Nitrogen Application Prescription System (NAPS) was developed. The initial NAPS setup requires long term recorded data (yield, weather, and soil) to ensure proper MM calibration. After calibration, the current growing season conditions are required (weather, management information) until the N application date. Afterward, the NAPS incorporates remote sensing information and generated weather for running future N application scenarios. The selection of the proper amount of N is determined by economic and ecological criteria. Furthermore, in order to account for differences in in-field variabilities and to deliver a N prescription site-specifically, the NAPS concept has to be applied on a geospatial scale by adjusting soil parameters spatially. The NAPS concept has the potential to adjust the N application more economically and ecologically by using current sensor data, historical yield records, and future weather prediction to derive a more precise N application strategy. Finally, this concept exhibits the potential for reconciliation of the issue of an economic, agricultural production without harming the environment.In dieser Arbeit wurde eruiert, ob mit Hilfe von Sensordaten und Pflanzenwachstumsmodellen eine N-Düngemittelstrategie abgeleitet werden kann, die in der Lage ist die ökologischen Belastung zu verringern. Dies umfasste die Evaluation folgender Fragestellungen: (I) Wird die spektrale Reflexion und somit die Bestimmung der N-Konzentration durch die Messung an verschiedenen Blattetagen und -Positionen beeinflusst (Publikation I)? Für die Klärung dieser ersten Frage wurden in zwei hydroponischen Gewächshausversuchen Weizenpflanzen bei unterschiedlicher N-Exposition und Trockenstress kultiviert. Für jede Pflanze wurden spektrale Reflexionsmessungen an drei Blattetagen und an drei Positionen auf dem Blatt durchgeführt. Insgesamt wurden die 16 üblichsten auf spektraler Reflexion basierenden Vegetationsindizes für jede Kombination von Blattetage und -Position berechnet. Die N-Konzentration der Pflanze wurde durch Laboranalysen bestimmt. Weder die Position auf dem Blatt noch die Blattetage hatten einen Einfluss auf die Genauigkeit der Bestimmung der N-Konzentration der Pflanze durch spektrale Reflexionsmessungen. Daher sind Messungen auf Bestandsebene ausreichend. Falls jedoch weitere Stressfaktoren wie Trockenheit oder Krankheitsbefall auftreten, kann eine Differenzierung zwischen verschiedenen Blattetagen notwendig oder von Vorteil sein. In der nächsten Fragestellung (Publikation II) wurde untersucht, wie Blattkrankheiten in ein DSSAT-Weizenmodell integriert werden können, um so die Auswirkungen von Blattkrankheiten auf den Ertrag zu simulieren. Eine Modellerweiterung wurde entwickelt, durch die Integration eines Blattkrankheitsmoduls in das bestehende DSSAT Weizenmodell. Das Modul simuliert die Auswirkungen des täglichen Schadens durch die Krankheit auf die Photosynthese und den Blattflächenindex. Der Ansatz wurde an einem zweijährigen Datensatz aus Argentinien mit verschiedenen Weizensorten und an einem einjährigen Datensatz aus Deutschland mit verschiedenen Inokulumniveaus von Septoria tritici-Blotch (STB) getestet. Die Sensitivitätsanalyse zeigte die Möglichkeit des Modells, den Ertrag in einer exponentiellen Beziehung mit zunehmendem Infektionsgrad (0-70%) zu reduzieren. Das erweiterte Modell stellt somit eine Möglichkeit dar, STB-Infektionen standortspezifisch in Verbindung mit verfügbaren Sensordaten zu simulieren. Um die Modellierungsgenauigkeit noch weiter zu erhöhen, wurde der Einsatz eines MM-Ansatz geprüft. Die Kombination von verschiedenen Modellen erhöht die Komplexität der Simulation und des damit verbundenen Kalibrierungsverfahrens, insbesondere wenn der Benutzer nicht mit allen Modellen vertraut ist. Die dritte Fragestellung (iii) untersuchte daher, ob objektive Kalibrierungsergebnisse gewährleitet werden könnten, wenn die cultivar coefficients im Modell auf Basis tatsächlich gemessener Daten mittels eines neu entwickelten automatischen Calibrator-Programms optimiert wurden. Die Modellkalibrierung wurde an einem 4-jährigen-Weizendüngungsversuch in Südwestdeutschland durchgeführt. Die statistische Auswertung des Kalibrierverfahrens zeigte zufriedenstellende Ergebnisse und führte zur vierten Fragestellung. Die vierte Fragestellung befasste sich mit dem Thema, ob ein MM-Ansatz die Gesamtmodelliergenauigkeit verbessern kann. Die Auswertung des Düngemittelversuchs zeigte in den meisten Fällen eine verbesserte Modellierungsgenauigkeit, insbesondere in einem durch Wasserstress geprägten Versuchsjahr wie 2018. Unter Verwendung eines MM-Ansatzes, durch Anpassung der Modellvariablen und durch die Integration von Sensordaten wurde ein Nitrogen Application Prescription System (NAPS) entwickelt. Eine Voraussetzung für das NAPS-Konzepts ist das Vorhandensein von Langzeit-Daten (Ertrag, Klima- und Bodenbedingungen), um eine korrekte MM-Kalibrierung zu gewährleisten. Nach der Kalibrierung werden die Bedingungen der aktuellen Wachstumssaison (Wetter, Managementinformationen) bis zum Düngetermin benötigt. Anschließend berechnet das NAPS basierend auf Sensorinformationen und simulierten Wetterbedingungen verschiedene Düngeszenarien. Ökonomische und ökologische Kriterien bestimmen die optimierte Düngemenge. Darüber hinaus muss das NAPS-Konzept auf räumlicher Ebene arbeiten, indem es die Bodenparameter berücksichtigt. So kann unter Beachtung der Feldvariabilität eine standortspezifische N-Ausbringung gewährleistet werden. In Summe zeigte sich, dass NAPS die Düngung an ökonomische und ökologische Faktoren anpasst, indem es aktuelle Sensordaten, historische Ertragsaufzeichnungen und zukünftige Wettervorhersagen zur Ermittlung einer präziseren N-Ausbringung nutzt. Das Konzept hat so das Potenzial, die nachteiligen Auswirkungen einer Überdüngung zu begrenzen, so dass eine umweltfreundlichere Agrarproduktion gewährleistet wird