5,998 research outputs found

    UMSL Bulletin 2023-2024

    Get PDF
    The 2023-2024 Bulletin and Course Catalog for the University of Missouri St. Louis.https://irl.umsl.edu/bulletin/1088/thumbnail.jp

    Digitalization and Development

    Get PDF
    This book examines the diffusion of digitalization and Industry 4.0 technologies in Malaysia by focusing on the ecosystem critical for its expansion. The chapters examine the digital proliferation in major sectors of agriculture, manufacturing, e-commerce and services, as well as the intermediary organizations essential for the orderly performance of socioeconomic agents. The book incisively reviews policy instruments critical for the effective and orderly development of the embedding organizations, and the regulatory framework needed to quicken the appropriation of socioeconomic synergies from digitalization and Industry 4.0 technologies. It highlights the importance of collaboration between government, academic and industry partners, as well as makes key recommendations on how to encourage adoption of IR4.0 technologies in the short- and long-term. This book bridges the concepts and applications of digitalization and Industry 4.0 and will be a must-read for policy makers seeking to quicken the adoption of its technologies

    UMSL Bulletin 2022-2023

    Get PDF
    The 2022-2023 Bulletin and Course Catalog for the University of Missouri St. Louis.https://irl.umsl.edu/bulletin/1087/thumbnail.jp

    Investigation of the metabolism of rare nucleotides in plants

    Get PDF
    Nucleotides are metabolites involved in primary metabolism, and specialized metabolism and have a regulatory role in various biochemical reactions in all forms of life. While in other organisms, the nucleotide metabolome was characterized extensively, comparatively little is known about the cellular concentrations of nucleotides in plants. The aim of this dissertation was to investigate the nucleotide metabolome and enzymes influencing the composition and quantities of nucleotides in plants. For this purpose, a method for the analysis of nucleotides and nucleosides in plants and algae was developed (Chapter 2.1), which comprises efficient quenching of enzymatic activity, liquid-liquid extraction and solid phase extraction employing a weak-anionexchange resin. This method allowed the analysis of the nucleotide metabolome of plants in great depth including the quantification of low abundant deoxyribonucleotides and deoxyribonucleosides. The details of the method were summarized in an article, serving as a laboratory protocol (Chapter 2.2). Furthermore, we contributed a review article (Chapter 2.3) that summarizes the literature about nucleotide analysis and recent technological advances with a focus on plants and factors influencing and hindering the analysis of nucleotides in plants, i.e., a complex metabolic matrix, highly stable phosphatases and physicochemical properties of nucleotides. To analyze the sub-cellular concentrations of metabolites, a protocol for the rapid isolation of highly pure mitochondria utilizing affinity chromatography was developed (Chapter 2.4). The method for the purification of nucleotides furthermore contributed to the comprehensive analysis of the nucleotide metabolome in germinating seeds and in establishing seedlings of A. thaliana, with a focus on genes involved in the synthesis of thymidilates (Chapter 2.5) and the characterization of a novel enzyme of purine nucleotide degradation, the XANTHOSINE MONOPHOSPHATE PHOSPHATASE (Chapter 2.6). Protein homology analysis comparing A. thaliana, S. cerevisiae, and H. sapiens led to the identification and characterization of an enzyme involved in the metabolite damage repair system of plants, the INOSINE TRIPHOSPHATE PYROPHOSPHATASE (Chapter 2.7). It was shown that this enzyme dephosphorylates deaminated purine nucleotide triphosphates and thus prevents their incorporation into nucleic acids. Lossof-function mutants senesce early and have a constitutively increased content of salicylic acid. Also, the source of deaminated purine nucleotides in plants was investigated and it was shown that abiotic factors contribute to nucleotide damage.Nukleotide sind Metaboliten, die am Primärstoffwechsel und an spezialisierten Stoffwechselvorgängen beteiligt sind und eine regulierende Rolle bei verschiedenen biochemischen Reaktionen in allen Lebensformen spielen. Während bei anderen Organismen das Nukleotidmetabolom umfassend charakterisiert wurde, ist in Pflanzen vergleichsweise wenig über die zellulären Konzentrationen von Nukleotiden bekannt. Ziel dieser Dissertation war es, das Nukleotidmetabolom und die Enzyme zu untersuchen, die die Zusammensetzung und Menge der Nukleotide in Pflanzen beeinflussen. Zu diesem Zweck wurde eine Methode zur Analyse von Nukleotiden und Nukleosiden in Pflanzen und Algen entwickelt (Kapitel 2.1), die ein effizientes Stoppen enzymatischer Aktivität, eine Flüssig-Flüssig-Extraktion und eine Festphasenextraktion unter Verwendung eines schwachen Ionenaustauschers umfasst. Mit dieser Methode konnte das Nukleotidmetabolom von Pflanzen eingehend analysiert werden, einschließlich der Quantifizierung von Desoxyribonukleotiden und Desoxyribonukleosiden mit geringer Abundanz. Die Einzelheiten der Methode wurden in einem Artikel zusammengefasst, der als Laborprotokoll dient (Kapitel 2.2). Darüber hinaus wurde ein Übersichtsartikel (Kapitel 2.3) verfasst, der die Literatur über die Analyse von Nukleotiden und die jüngsten technologischen Fortschritte zusammenfasst. Der Schwerpunkt lag hierbei auf Pflanzen und Faktoren, die die Analyse von Nukleotiden in Pflanzen beeinflussen oder behindern, d. h. eine komplexe Matrix, hochstabile Phosphatasen und physikalisch-chemische Eigenschaften von Nukleotiden. Um die subzellulären Konzentrationen von Metaboliten zu analysieren, wurde ein Protokoll für die schnelle Isolierung hochreiner Mitochondrien unter Verwendung einer Affinitätschromatographie entwickelt (Kapitel 2.4). Die Methode zur Analyse von Nukleotiden trug außerdem zu einer umfassenden Analyse des Nukleotidmetaboloms in keimenden Samen und in sich etablierenden Keimlingen von A. thaliana bei, wobei der Schwerpunkt auf Genen lag, die an der Synthese von Thymidilaten beteiligt sind (Kapitel 2.5), sowie zu der Charakterisierung eines neuen Enzyms des Purinnukleotidabbaus, der XANTHOSINE MONOPHOSPHATE PHOSPHATASE (Kapitel 2.6). Eine Proteinhomologieanalyse, die A. thaliana, S. cerevisiae und H. sapiens miteinander verglich führte zur Identifizierung und Charakterisierung eines Enzyms, das an der Reparatur von geschädigten Metaboliten in Pflanzen beteiligt ist, der INOSINE TRIPHOSPHATE PYROPHOSPHATASE (Kapitel 2.7). Es konnte gezeigt werden, dass dieses Enzym desaminierte Purinnukleotidtriphosphate dephosphoryliert und so deren Einbau in Nukleinsäuren verhindert. Funktionsverlustmutanten altern früh und weisen einen konstitutiv erhöhten Gehalt an Salicylsäure auf. Außerdem wurde die Quelle der desaminierten Purinnukleotide in Pflanzen untersucht, und es wurde gezeigt, dass abiotische Faktoren zur Nukleotidschädigung beitragen

    Effects of municipal smoke-free ordinances on secondhand smoke exposure in the Republic of Korea

    Get PDF
    ObjectiveTo reduce premature deaths due to secondhand smoke (SHS) exposure among non-smokers, the Republic of Korea (ROK) adopted changes to the National Health Promotion Act, which allowed local governments to enact municipal ordinances to strengthen their authority to designate smoke-free areas and levy penalty fines. In this study, we examined national trends in SHS exposure after the introduction of these municipal ordinances at the city level in 2010.MethodsWe used interrupted time series analysis to assess whether the trends of SHS exposure in the workplace and at home, and the primary cigarette smoking rate changed following the policy adjustment in the national legislation in ROK. Population-standardized data for selected variables were retrieved from a nationally representative survey dataset and used to study the policy action’s effectiveness.ResultsFollowing the change in the legislation, SHS exposure in the workplace reversed course from an increasing (18% per year) trend prior to the introduction of these smoke-free ordinances to a decreasing (−10% per year) trend after adoption and enforcement of these laws (β2 = 0.18, p-value = 0.07; β3 = −0.10, p-value = 0.02). SHS exposure at home (β2 = 0.10, p-value = 0.09; β3 = −0.03, p-value = 0.14) and the primary cigarette smoking rate (β2 = 0.03, p-value = 0.10; β3 = 0.008, p-value = 0.15) showed no significant changes in the sampled period. Although analyses stratified by sex showed that the allowance of municipal ordinances resulted in reduced SHS exposure in the workplace for both males and females, they did not affect the primary cigarette smoking rate as much, especially among females.ConclusionStrengthening the role of local governments by giving them the authority to enact and enforce penalties on SHS exposure violation helped ROK to reduce SHS exposure in the workplace. However, smoking behaviors and related activities seemed to shift to less restrictive areas such as on the streets and in apartment hallways, negating some of the effects due to these ordinances. Future studies should investigate how smoke-free policies beyond public places can further reduce the SHS exposure in ROK

    Science and Innovations for Food Systems Transformation

    Get PDF
    This Open Access book compiles the findings of the Scientific Group of the United Nations Food Systems Summit 2021 and its research partners. The Scientific Group was an independent group of 28 food systems scientists from all over the world with a mandate from the Deputy Secretary-General of the United Nations. The chapters provide science- and research-based, state-of-the-art, solution-oriented knowledge and evidence to inform the transformation of contemporary food systems in order to achieve more sustainable, equitable and resilient systems

    Book of abstracts II International Congress - CIEQV 23rd and 24th February 2023

    Get PDF
    The II International Congress - CIEQV is the consequence of the development of the center’s strategic plan, which aims to increase scientific production in the field of quality of life. The communication of the science carried out configures a relevant social approach for public scientific research institutions. This congress aims above all to make available the knowledge acquired on the subject of quality of life, aiming to transfer to society and citizens the information applied to their way of life. We consider the highlights of the congress to be the discussion at roundtables on two fracturing themes, inclusion and urbanism associated with the quality of life of citizens. The conferences are multidisciplinary, addressing topics such as children’s education and sports participation, sustainable agriculture and self-care, all of them related to citizens’ quality of life. The proposed papers reflect the diverse research paradigms of this multidisciplinary and thematic center: studies on the educational contexts of youth and children; on the inclusion of people with disabilities; research focused on food products and sustainable behaviors; approaches to sports training, athlete performances, physical activity practice; exploration of research on the motor behavior of children and youth; study of sustainability in organizations; research focused on quality of life in the different stages of human life; etc. This diverse approach demonstrates the complexity of the social phenomenon under study. Researchers search the connection between some variables and their interrelationship. In this way, the research produced gets closer to the ecological and social reality, transferring itself to human practices towards a better and healthy quality of life. This II International Congress - CIEQV is another initiative for the challenges of research in Quality of Life. I take this opportunity to thank the institutions that support us, the organizers of the event and the researchers who share their knowledge, from the perspective of open science and available to society.info:eu-repo/semantics/publishedVersio

    Galanin neurons modulate stress responses in zebrafish larvae

    Get PDF
    Stress responses are fundamental for the survival of an organism and constitute an adaptive reaction to a real or perceived threat. Neuronal, endocrine, and behavioral responses are activated after the perception of a stressor and are aimed to re-establish the organism's homeostasis. A complex network of neuronal circuits involving several brain regions orchestrates the activation of the major stress system, the Hypothalamic Pituitary Adrenal (HPA) axis, and most of the neuronal populations that control its activity reside in hypothalamic nuclei. Activation of the HPA axis starts within the paraventricular nucleus of the hypothalamus, in which a population of peptidergic neurons secretes corticotropin-releasing hormone (CRH) into the circulation, which in turn initiates a signaling cascade culminating with the production of cortisol. The activity of the HPA axis and CRH-producing neurons must be tightly controlled and rapidly modulated to ensure proper responses to threats but also to avoid overactivation of the stress system, which is deleterious for an animal’s wellbeing. However, the hypothalamic circuits responsible for the activation and termination of the stress response and the underlying neuromodulatory mechanisms are still largely unknown. In this study, I used the zebrafish larva as a model to elucidate the hypothalamic neuronal circuits modulating the behavioral and neuroendocrine responses to acute stress. The transparent and small larval zebrafish brain provides the unique opportunity to study neuronal responses in vivo, facilitating the identification of the neuronal populations modulating stress. Importantly the main brain regions and stress axes are conserved between mammals and teleosts. The Hypothalamic Pituitary Interrenal (HPI) axis is homologous to the mammalian HPA axis and its activation is controlled by Crh-producing neurons located in the preoptic area (PoA), homologous to the mammalian paraventricular nucleus. Among the peptidergic neurons of the hypothalamus that might be involved in the modulation of acute stress, I chose to focus on a population of cells secreting the neuropeptide Galanin (Galn). I identified in the PoA of zebrafish a subpopulation of Galanin-producing neurons (Galn+) highly responsive to different types of stressful stimuli. Ablation of Galn+ neurons led to exacerbated stress responses, elevated cortisol levels, and caused increased activation of Crh-producing neurons, suggesting an inhibitory effect of Galn+ neurons over the HPI axis. I also found that Galn+ neurons in the PoA of zebrafish larvae are GABAergic, suggesting the possibility that GABA is the neurotransmitter released by Galn+ neurons to inhibit downstream stress-promoting circuits. I further investigated the molecular mechanisms by which Galn+ neurons negatively modulate stress responses by manipulating the peptide Galn. Lack of Galn elicited a diminished response to stress and increased the activity of Galn+ neurons in the PoA. Conversely, overexpression of Galn exacerbated stress- related responses and decreased the activity of Galn+ neurons, suggesting a self-inhibitory action of Galn peptide on Galn+ neurons. Taken together, the results reported in this study indicate that Galn+ neurons in the PoA negatively modulate Crh+ neurons, likely through GABAergic transmission, to prevent overactivation of the HPI axis. In parallel, the neuropeptide Galn mediates an additional modulatory control within this hypothalamic circuit, reducing the activity of Galn+ neurons through an autocrine mechanism. This dual system likely regulates a balance of activation and inhibition over Crh+ neurons, which allows fine-tuning of the HPI axis activity and mediate behavioral responses to stress

    Deep learning for intracellular particle tracking and motion analysis

    Get PDF
    • …
    corecore