9,688 research outputs found

    Quantitative volumetric Raman imaging of three dimensional cell cultures

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    The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment. We demonstrate visualization and quantification of fine details in 3D cell shape, cytoplasm, nucleus, lipid bodies and cytoskeletal structures in 3D with unprecedented biomolecular specificity for vibrational microspectroscopy

    Coherent Raman spectro-imaging with laser frequency combs

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    Optical spectroscopy and imaging of microscopic samples have opened up a wide range of applications throughout the physical, chemical, and biological sciences. High chemical specificity may be achieved by directly interrogating the fundamental or low-lying vibrational energy levels of the compound molecules. Amongst the available prevailing label-free techniques, coherent Raman scattering has the distinguishing features of high spatial resolution down to 200 nm and three-dimensional sectioning. However, combining fast imaging speed and identification of multiple - and possibly unexpected- compounds remains challenging: existing high spectral resolution schemes require long measurement times to achieve broad spectral spans. Here we overcome this difficulty and introduce a novel concept of coherent anti-Stokes Raman scattering (CARS) spectro-imaging with two laser frequency combs. We illustrate the power of our technique with high resolution (4 cm-1) Raman spectra spanning more than 1200 cm-1 recorded within less than 15 microseconds. Furthermore, hyperspectral images combining high spectral (10 cm-1) and spatial (2 micrometers) resolutions are acquired at a rate of 50 pixels per second. Real-time multiplex accessing of hyperspectral images may dramatically expand the range of applications of nonlinear microscopy.Comment: 8 pages, 3 figure

    Seafloor characterization using airborne hyperspectral co-registration procedures independent from attitude and positioning sensors

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    The advance of remote-sensing technology and data-storage capabilities has progressed in the last decade to commercial multi-sensor data collection. There is a constant need to characterize, quantify and monitor the coastal areas for habitat research and coastal management. In this paper, we present work on seafloor characterization that uses hyperspectral imagery (HSI). The HSI data allows the operator to extend seafloor characterization from multibeam backscatter towards land and thus creates a seamless ocean-to-land characterization of the littoral zone
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