Efficient Two-Stage Group Testing Algorithms for Genetic Screening

Efficient two-stage group testing algorithms that are particularly suited for rapid and less-expensive DNA library screening and other large scale biological group testing efforts are investigated in this paper. The main focus is on novel combinatorial constructions in order to minimize the number of individual tests at the second stage of a two-stage disjunctive testing procedure. Building on recent work by Levenshtein (2003) and Tonchev (2008), several new infinite classes of such combinatorial designs are presented.Comment: 14 pages; to appear in "Algorithmica". Part of this work has been presented at the ICALP 2011 Group Testing Workshop; arXiv:1106.368

Noise-Resilient Group Testing: Limitations and Constructions

We study combinatorial group testing schemes for learning $d$-sparse Boolean vectors using highly unreliable disjunctive measurements. We consider an adversarial noise model that only limits the number of false observations, and show that any noise-resilient scheme in this model can only approximately reconstruct the sparse vector. On the positive side, we take this barrier to our advantage and show that approximate reconstruction (within a satisfactory degree of approximation) allows us to break the information theoretic lower bound of $\tilde{\Omega}(d^2 \log n)$ that is known for exact reconstruction of $d$-sparse vectors of length $n$ via non-adaptive measurements, by a multiplicative factor $\tilde{\Omega}(d)$. Specifically, we give simple randomized constructions of non-adaptive measurement schemes, with $m=O(d \log n)$ measurements, that allow efficient reconstruction of $d$-sparse vectors up to $O(d)$ false positives even in the presence of $\delta m$ false positives and $O(m/d)$ false negatives within the measurement outcomes, for any constant $\delta < 1$. We show that, information theoretically, none of these parameters can be substantially improved without dramatically affecting the others. Furthermore, we obtain several explicit constructions, in particular one matching the randomized trade-off but using $m = O(d^{1+o(1)} \log n)$ measurements. We also obtain explicit constructions that allow fast reconstruction in time \poly(m), which would be sublinear in $n$ for sufficiently sparse vectors. The main tool used in our construction is the list-decoding view of randomness condensers and extractors.Comment: Full version. A preliminary summary of this work appears (under the same title) in proceedings of the 17th International Symposium on Fundamentals of Computation Theory (FCT 2009

Revisiting nested group testing procedures: new results, comparisons, and robustness

Group testing has its origin in the identification of syphilis in the US army during World War II. Much of the theoretical framework of group testing was developed starting in the late 1950s, with continued work into the 1990s. Recently, with the advent of new laboratory and genetic technologies, there has been an increasing interest in group testing designs for cost saving purposes. In this paper, we compare different nested designs, including Dorfman, Sterrett and an optimal nested procedure obtained through dynamic programming. To elucidate these comparisons, we develop closed-form expressions for the optimal Sterrett procedure and provide a concise review of the prior literature for other commonly used procedures. We consider designs where the prevalence of disease is known as well as investigate the robustness of these procedures when it is incorrectly assumed. This article provides a technical presentation that will be of interest to researchers as well as from a pedagogical perspective. Supplementary material for this article is available online.Comment: Submitted for publication on May 3, 2016. Revised versio

Improved Combinatorial Group Testing Algorithms for Real-World Problem Sizes

We study practically efficient methods for performing combinatorial group testing. We present efficient non-adaptive and two-stage combinatorial group testing algorithms, which identify the at most d items out of a given set of n items that are defective, using fewer tests for all practical set sizes. For example, our two-stage algorithm matches the information theoretic lower bound for the number of tests in a combinatorial group testing regimen.Comment: 18 pages; an abbreviated version of this paper is to appear at the 9th Worksh. Algorithms and Data Structure

Group Testing with Probabilistic Tests: Theory, Design and Application

Identification of defective members of large populations has been widely studied in the statistics community under the name of group testing. It involves grouping subsets of items into different pools and detecting defective members based on the set of test results obtained for each pool. In a classical noiseless group testing setup, it is assumed that the sampling procedure is fully known to the reconstruction algorithm, in the sense that the existence of a defective member in a pool results in the test outcome of that pool to be positive. However, this may not be always a valid assumption in some cases of interest. In particular, we consider the case where the defective items in a pool can become independently inactive with a certain probability. Hence, one may obtain a negative test result in a pool despite containing some defective items. As a result, any sampling and reconstruction method should be able to cope with two different types of uncertainty, i.e., the unknown set of defective items and the partially unknown, probabilistic testing procedure. In this work, motivated by the application of detecting infected people in viral epidemics, we design non-adaptive sampling procedures that allow successful identification of the defective items through a set of probabilistic tests. Our design requires only a small number of tests to single out the defective items. In particular, for a population of size $N$ and at most $K$ defective items with activation probability $p$, our results show that $M = O(K^2\log{(N/K)}/p^3)$ tests is sufficient if the sampling procedure should work for all possible sets of defective items, while $M = O(K\log{(N)}/p^3)$ tests is enough to be successful for any single set of defective items. Moreover, we show that the defective members can be recovered using a simple reconstruction algorithm with complexity of $O(MN)$.Comment: Full version of the conference paper "Compressed Sensing with Probabilistic Measurements: A Group Testing Solution" appearing in proceedings of the 47th Annual Allerton Conference on Communication, Control, and Computing, 2009 (arXiv:0909.3508). To appear in IEEE Transactions on Information Theor

GROTESQUE: Noisy Group Testing (Quick and Efficient)

Group-testing refers to the problem of identifying (with high probability) a (small) subset of $D$ defectives from a (large) set of $N$ items via a "small" number of "pooled" tests. For ease of presentation in this work we focus on the regime when D = \cO{N^{1-\gap}} for some \gap > 0. The tests may be noiseless or noisy, and the testing procedure may be adaptive (the pool defining a test may depend on the outcome of a previous test), or non-adaptive (each test is performed independent of the outcome of other tests). A rich body of literature demonstrates that $\Theta(D\log(N))$ tests are information-theoretically necessary and sufficient for the group-testing problem, and provides algorithms that achieve this performance. However, it is only recently that reconstruction algorithms with computational complexity that is sub-linear in $N$ have started being investigated (recent work by \cite{GurI:04,IndN:10, NgoP:11} gave some of the first such algorithms). In the scenario with adaptive tests with noisy outcomes, we present the first scheme that is simultaneously order-optimal (up to small constant factors) in both the number of tests and the decoding complexity (\cO{D\log(N)} in both the performance metrics). The total number of stages of our adaptive algorithm is "small" (\cO{\log(D)}). Similarly, in the scenario with non-adaptive tests with noisy outcomes, we present the first scheme that is simultaneously near-optimal in both the number of tests and the decoding complexity (via an algorithm that requires \cO{D\log(D)\log(N)} tests and has a decoding complexity of {${\cal O}(D(\log N+\log^{2}D))$}. Finally, we present an adaptive algorithm that only requires 2 stages, and for which both the number of tests and the decoding complexity scale as {${\cal O}(D(\log N+\log^{2}D))$}. For all three settings the probability of error of our algorithms scales as \cO{1/(poly(D)}.Comment: 26 pages, 5 figure

Optimal Nested Test Plan for Combinatorial Quantitative Group Testing

We consider the quantitative group testing problem where the objective is to identify defective items in a given population based on results of tests performed on subsets of the population. Under the quantitative group testing model, the result of each test reveals the number of defective items in the tested group. The minimum number of tests achievable by nested test plans was established by Aigner and Schughart in 1985 within a minimax framework. The optimal nested test plan offering this performance, however, was not obtained. In this work, we establish the optimal nested test plan in closed form. This optimal nested test plan is also order optimal among all test plans as the population size approaches infinity. Using heavy-hitter detection as a case study, we show via simulation examples orders of magnitude improvement of the group testing approach over two prevailing sampling-based approaches in detection accuracy and counter consumption. Other applications include anomaly detection and wideband spectrum sensing in cognitive radio systems