MMP-3 deficiency alleviates endotoxin-induced acute inflammation in the posterior eye segment

Matrix metalloproteinase-3 (MMP-3) is known to mediate neuroinflammatory processes by activating microglia, disrupting blood-central nervous system barriers and supporting neutrophil influx into the brain. In addition, the posterior part of the eye, more specifically the retina, the retinal pigment epithelium (RPE) and the blood-retinal barrier, is affected upon neuroinflammation, but a role for MMP-3 during ocular inflammation remains elusive. We investigated whether MMP-3 contributes to acute inflammation in the eye using the endotoxin-induced uveitis (EIU) model. Systemic administration of lipopolysaccharide induced an increase in MMP-3 mRNA and protein expression level in the posterior part of the eye. MMP-3 deficiency or knockdown suppressed retinal leukocyte adhesion and leukocyte infiltration into the vitreous cavity in mice subjected to EIU. Moreover, retinal and RPE mRNA levels of intercellular adhesion molecule 1 (Icam1), interleukin 6 (Il6), cytokine-inducible nitrogen oxide synthase (Nos2) and tumor necrosis factor alpha (Tnf alpha), which are key molecules involved in EIU, were clearly reduced in MMP-3 deficient mice. In addition, loss of MMP-3 repressed the upregulation of the chemokines monocyte chemoattractant protein (MCP)-1 and (C-X-C motif) ligand 1 (CXCL1). These findings suggest a contribution of MMP-3 during EIU, and its potential use as a therapeutic drug target in reducing ocular inflammation

A Musical Journey Through Time

Performend by the Eastern Symphony Orchestra and EIU Choral Ensembles with special appearances by the EIU Mixed Chorus, the EIU Concert Choir, the EIU Oratorio Society, and the Collegium Musicum Ensembles. Featuring Derek Maninflor performing Tchaikovsky\u27s Piano Concerto No. 1 in B-flat Minor, Opus 23 . Conducted by Richard Robert Rossi.https://thekeep.eiu.edu/music_concerts/1050/thumbnail.jp

A Musical Journey Through Time

Performend by the Eastern Symphony Orchestra and EIU Choral Ensembles with special appearances by the EIU Mixed Chorus, the EIU Concert Choir, the EIU Oratorio Society, and the Collegium Musicum Ensembles. Featuring Derek Maninflor performing Tchaikovsky\u27s Piano Concerto No. 1 in B-flat Minor, Opus 23 . Conducted by Richard Robert Rossi.https://thekeep.eiu.edu/music_concerts/1050/thumbnail.jp

Global Microscope on the Microfinance Business Environment 2010: An Index and Study by the Economist Intelligence Unit

This report outlines the findings of the Economist Intelligence Unit¿s in-depth analysis of the microfi nance business environment in 54 countries. The index that underlies this report allows countries and regions to be compared across three broad categories: regulatory framework, institutional development and investment climate. The study uses a methodology which was originally developed for Latin America and the Caribbean in 2007 and was employed for the fi rst time on a global basis in 2009. Most of the research for this report was conducted prior to May 2010, although some later developments (up to July) were included where they were particularly signifi cant.Microbusinesses & Microfinance

Eastern Alumnus Vol. 32 No. 2 (Winter 1978-79)

EIU Grad Gets Commerce Dept. Gold Medal; Distinguished Alumni Named; EIU\u27s Marching Blue; etc.https://thekeep.eiu.edu/eiu_alumni_news_1978/1003/thumbnail.jp

Does Television Impact Attendance at Eastern Illinois University Sporting Events

This study tested the relationship between Eastern Illinois University home football and basketball games on television and attendance at these events. Attendance during a ten-year period for both EIU football and basketball games was recorded and dates of these contests were researched to determine if the games were telecast. After categorizing the figures into eight groups, the four pairs were compared using a T-test. The study found televising EIU home football games had no significant impact on attendance at these contests. However, a significant difference was noted between EIU home basketball games televised and those not aired. Attendance actually increased when EIU home basketball games were televised. These mixed findings are quite comparable to other studies and popular wisdom, yet the basketball results yield a much more common philosophy within EIU\u27s sports administration department

Requiem by Mozart

Performed by the EIU Mixed Chorus, the EIU Oratorio Society, and EIU Concerto winner Connie Ignatiou (oboe). Featuring Soprano Ollie Watts Davis, Mezzo-Soprano Marilyn Coles, Tenor Jerold Siena, and Bass Ronald Hedlund. Conducted by Richard Robert Rossi.https://thekeep.eiu.edu/music_concerts/1055/thumbnail.jp

Hubungan Polimorfisme Gen Me2 dengan Epilepsi Idiopatik Umum dan Respon Terapi Valproat

Penelitian ini bertujuan untuk: (1) Mendeteksipolimorfisme rs585344, rs645088 dan rs642698, genME2 pada EIU anak tanpa epilepsi untuk menentukanadanya hubungan polimorfime gen ME2 denganEIU. (2) Menentukan jumlah proporsi hilangnyabangkitan 6 bulan setelah pengobatan valproat padaEIU dengan genotipe mutan dan genotip bukanmutan polimorfisme rs585344, rs645088 dan rs642698gen ME2. Penelitian kasus-kontrol dilakukan selamaperiode Juli 2010 sampai April 2012 terhadap 92subjek EIU usia awitan anak dan remaja di RS HasanSadikin Bandung, RS Cipto Mangunkusumo Jakartadan RS St Borromeus Bandung, 336 subjek kontrol diRS Hasan Sadikin Bandung. Hasil penelitian denganuji chi-kuadrat menunjukkan perbedaan bermaknaproporsi genotip polimorfisme rs585344 gen ME2antara EIU usia awitan anak dan remaja dengankontrol (p 0,05). Didapatkan perbedaan bermakna genotipmutan polimorfisme rs585344, rs645088 dan rs642698gen ME2 EIU usia awitan anak < 11 tahun dengangenotip mutan polimorfisme rs585344, rs645088 danrs642698 gen ME2 EIU usia awitan anak ≥ 11 tahun(age-dependent penetrance)

Eastern Alumnus Vol. 31 No. 4 (Summer 1978)

Lord Scholars Named; Harry Fitzhugh Retires; Honorary Degrees at EIU; Thanks, Decatur! - EIU Telefund a success; etc.https://thekeep.eiu.edu/eiu_alumni_news_1977/1006/thumbnail.jp

Mast cells modulate the inflammatory process in endotoxin-induced uveitis

Purpose: To investigate the role of mast cells and annexin-A1 (Anxa1) in endotoxin-induced uveitis (EIU). Methods: EIU was induced by injection of lipopolysaccharide (LPS) into the paws of rats, which were then sacrificed after 24 and 48 h. To assess EIU in the absence of mast cells, groups of animals were pretreated with compound 48/80 (c48/80) and sacrificed after 24 h after no treatment or EIU induction. The eyes were used for histological studies and the aqueous humor (AqH) pool was used for the analysis of transmigrated cells and Anxa1 levels. In inflammatory cells, Anxa1 expression was monitored by immunohistochemistry. Results: After 24 h, rats with EIU exhibited degranulated mast cells, associated with elevated numbers of infiltrating leukocytes and the high expression of Anxa1 in the AqH and the neutrophils. After 48 h of EIU, the mast cells were intact, indicating granule re-synthesis, and there was a reduction of neutrophil transmigration and an increase in the number of mononuclear phagocytic cells in ocular tissues. Anxa1 expression was decreased in neutrophils but increased in mononuclear phagocytic cells. In the animals pretreated with c48/80 and subjected to EIU, mast cells responded to this secretagogue by degranulating and few transmigrated neutrophils were observed. Conclustions: We report that mast cells are a potential source of pharmacological mediators that are strongly linked to the pathophysiology of EIU, and the endogenous protein Anxa1 is a mediator in the homeostasis of the inflammatory process with anti-migratory effects on leukocytes, which supports further studies of this protein as an innovative therapy for uveitis. © 2011 Molecular Vision