Automated segmentation of tissue images for computerized IHC analysis

This paper presents two automated methods for the segmentation ofimmunohistochemical tissue images that overcome the limitations of themanual approach aswell as of the existing computerized techniques. The first independent method, based on unsupervised color clustering, recognizes automatically the target cancerous areas in the specimen and disregards the stroma; the second method, based on colors separation and morphological processing, exploits automated segmentation of the nuclear membranes of the cancerous cells. Extensive experimental results on real tissue images demonstrate the accuracy of our techniques compared to manual segmentations; additional experiments show that our techniques are more effective in immunohistochemical images than popular approaches based on supervised learning or active contours. The proposed procedure can be exploited for any applications that require tissues and cells exploration and to perform reliable and standardized measures of the activity of specific proteins involved in multi-factorial genetic pathologie

Region-based representations of image and video: segmentation tools for multimedia services

This paper discusses region-based representations of image and video that are useful for multimedia services such as those supported by the MPEG-4 and MPEG-7 standards. Classical tools related to the generation of the region-based representations are discussed. After a description of the main processing steps and the corresponding choices in terms of feature spaces, decision spaces, and decision algorithms, the state of the art in segmentation is reviewed. Mainly tools useful in the context of the MPEG-4 and MPEG-7 standards are discussed. The review is structured around the strategies used by the algorithms (transition based or homogeneity based) and the decision spaces (spatial, spatio-temporal, and temporal). The second part of this paper proposes a partition tree representation of images and introduces a processing strategy that involves a similarity estimation step followed by a partition creation step. This strategy tries to find a compromise between what can be done in a systematic and universal way and what has to be application dependent. It is shown in particular how a single partition tree created with an extremely simple similarity feature can support a large number of segmentation applications: spatial segmentation, motion estimation, region-based coding, semantic object extraction, and region-based retrieval.Peer ReviewedPostprint (published version

Spoken content retrieval: A survey of techniques and technologies

Speech media, that is, digital audio and video containing spoken content, has blossomed in recent years. Large collections are accruing on the Internet as well as in private and enterprise settings. This growth has motivated extensive research on techniques and technologies that facilitate reliable indexing and retrieval. Spoken content retrieval (SCR) requires the combination of audio and speech processing technologies with methods from information retrieval (IR). SCR research initially investigated planned speech structured in document-like units, but has subsequently shifted focus to more informal spoken content produced spontaneously, outside of the studio and in conversational settings. This survey provides an overview of the field of SCR encompassing component technologies, the relationship of SCR to text IR and automatic speech recognition and user interaction issues. It is aimed at researchers with backgrounds in speech technology or IR who are seeking deeper insight on how these fields are integrated to support research and development, thus addressing the core challenges of SCR