5,426 research outputs found
Image informatics strategies for deciphering neuronal network connectivity
Brain function relies on an intricate network of highly dynamic neuronal connections that rewires dramatically under the impulse of various external cues and pathological conditions. Among the neuronal structures that show morphologi- cal plasticity are neurites, synapses, dendritic spines and even nuclei. This structural remodelling is directly connected with functional changes such as intercellular com- munication and the associated calcium-bursting behaviour. In vitro cultured neu- ronal networks are valuable models for studying these morpho-functional changes. Owing to the automation and standardisation of both image acquisition and image analysis, it has become possible to extract statistically relevant readout from such networks. Here, we focus on the current state-of-the-art in image informatics that enables quantitative microscopic interrogation of neuronal networks. We describe the major correlates of neuronal connectivity and present workflows for analysing them. Finally, we provide an outlook on the challenges that remain to be addressed, and discuss how imaging algorithms can be extended beyond in vitro imaging studies
Remote Sensing Image Classification Using Attribute Filters Defined over the Tree of Shapes
International audience—Remotely sensed images with very high spatial resolution provide a detailed representation of the surveyed scene with a geometrical resolution that at the present can be up to 30 cm (WorldView-3). A set of powerful image processing operators have been defined in the mathematical morphology framework. Among those, connected operators (e.g., attribute filters) have proven their effectiveness in processing very high resolution images. Attribute filters are based on attributes which can be efficiently implemented on tree-based image representations. In this work, we considered the definition of min, max, direct and subtractive filter rules for the computation of attribute filters over the tree of shapes representation. We study their performance on the classification of remotely sensed images. We compare the classification results over the tree of shapes with the results obtained when the same rules are applied on the component trees. The random forest is used as a baseline classifier and the experiments are conducted using multispectral data sets acquired by QuickBird and IKONOS sensors over urban areas
On morphological hierarchical representations for image processing and spatial data clustering
Hierarchical data representations in the context of classi cation and data
clustering were put forward during the fties. Recently, hierarchical image
representations have gained renewed interest for segmentation purposes. In this
paper, we briefly survey fundamental results on hierarchical clustering and
then detail recent paradigms developed for the hierarchical representation of
images in the framework of mathematical morphology: constrained connectivity
and ultrametric watersheds. Constrained connectivity can be viewed as a way to
constrain an initial hierarchy in such a way that a set of desired constraints
are satis ed. The framework of ultrametric watersheds provides a generic scheme
for computing any hierarchical connected clustering, in particular when such a
hierarchy is constrained. The suitability of this framework for solving
practical problems is illustrated with applications in remote sensing
Digital Image Processing Applications
Digital image processing can refer to a wide variety of techniques, concepts, and applications of different types of processing for different purposes. This book provides examples of digital image processing applications and presents recent research on processing concepts and techniques. Chapters cover such topics as image processing in medical physics, binarization, video processing, and more
Thin film notch filters as platforms for biological image processing
Many image processing operations involve the modification of the spatial
frequency content of images. Here we demonstrate object-plane spatial frequency
filtering utilizing the angular sensitivity of a commercial spectral bandstop
filter. This approach to all-optical image processing is shown to generate
real-time pseudo-3D images of transparent biological and other samples, such as
human cervical cancer cells. This work demonstrates the potential of non-local,
non-interferometric approaches to image processing for uses in label-free
biological cell imaging and dynamical monitoring.Comment: manuscript 14 pages, 5 figures, supplementary material 7 pages, 4
supplementary figure
Synthesis and characterisation of hydrogels with controlled microstructure and enhanced mechanical properties
For the application of advanced hydrogel-based artificial muscle systems, conventional polymeric hydrogels usually suffer from various limitations such as structural inhomogeneity and poor mechanical strengths. Thus, improving the mechanical strength of a specific hydrogel system while maintaining its other useful properties become increasingly important. In this project, three different approaches were employed to improve the mechanical properties of hydrogels though microstructural control, including physical cross-links, copolymerisation, and interpenetrating systems. Analytical tools such as FTIR and XRD were used to confirm the success of sample preparation. Morphological SEM characterisations were applied to reveal direct graphic information on hydrogels microstructures. Equilibrium water swelling tests as well as uniaxial compression measurements were conducted to evaluate the influences of various experimental parameters on the hydrogels water-holding and mechanical properties.
The physical cross-linker approach was proved to be successful since comparable swelling capacities and dramatically enhanced mechanical strength were achieved in nanocomposite systems in comparison with conventional chemically cross-linked gel systems, due to the presence of flexible cross-linking points and the multifunctional cross-linker role played by clay. The copolymerisation approach, both between two neutral monomers and between one neutral and the other ionic monomer, was unsuccessful in terms of mechanical property enhancement due to the low cross-linking density as a result of the dominate competition of copolymerisation rather than cross-lining kinetics. The interpenetrating approach was concluded as successful since hugely improved mechanical toughness and slightly reduced swelling capacities were observed in most IPN gel systems
A Review on Data Fusion of Multidimensional Medical and Biomedical Data
Data fusion aims to provide a more accurate description of a sample than any one source of data alone. At the same time, data fusion minimizes the uncertainty of the results by combining data from multiple sources. Both aim to improve the characterization of samples and might improve clinical diagnosis and prognosis. In this paper, we present an overview of the advances achieved over the last decades in data fusion approaches in the context of the medical and biomedical fields. We collected approaches for interpreting multiple sources of data in different combinations: image to image, image to biomarker, spectra to image, spectra to spectra, spectra to biomarker, and others. We found that the most prevalent combination is the image-to-image fusion and that most data fusion approaches were applied together with deep learning or machine learning methods
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