415 research outputs found

    In Situ Structure and Function Analysis of Nitrifying/Denitrifying Biofilms

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    Biofouling and its control for in situ lab-on-a-chip marine environmental sensors

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    Biofouling is the process by which biological organisms attach to surfaces in an aqueous environment. This occurs on nearly all surfaces in all natural aquatic environments, and can cause problems with the functioning of scientific equipment exposed to the marine environment for extended periods. At the National Oceanographic Centre in Southampton (NOCS), the Centre for Marine Microsystems (CMM) is developing lab-on-chip micro-sensors to monitor the chemical and biological environment in situ in the oceans. Due to the long periods (up to several months) that these sensors will be deployed, biofouling by microbial biofilms is an important concern for the efficient running of these sensors. The aim of this project was therefore to determine the potential level of fouling within the sensors and to investigate the potential use of low-concentration diffusible molecules (LCDMs) to remediate biofouling.Many of the sensors in development by CMM are designed to sense specific chemical species and they use various chemical reagents to achieve this. The effects of some of these reagents on the formation of biofilms by mixed marine communities were investigated. It was shown that Griess reagent and ortho-phthadialdehyde (OPA), used to sense nitrites and ammonium respectively, effectively stop biofilm formation by killing microorganisms before they can attach to surfaces.Biofouling on two different polymers, cyclic olefin copolymer (COC) and poly (methyl methacrylate) (PMMA), used in the construction of micro-sensors, was compared with biofouling on glass. No differences were observed between COC and PMMA, however a small but significant difference in surface coverage was observed between glass and COC at the early stages of exposure to the marine environment. The lack of differences between the two polymers suggests that biofouling is not an important consideration when deciding whether to construct sensors from COC or PMMA. However, the larger degree of fouling on hydrophobic COC compared with hydrophilic glass indicates a potential use of surface modifications as an antifouling strategy.The effects on biofouling of the LCDMs nitric oxide (NO), cis-2-decenoic acid (CDA) and patulin, were investigated to evaluate their potential for anti-fouling in marine micro sensors. All three molecules were shown to reduce the formation of biofilms by mixed marine communities, but colony counts suggested that the effect of patulin was due to toxicity as opposed to a physiological effect. Investigation of biofilm growth in the light and the dark revealed that there was less biofilm formation in the light that the dark and this effect was determined to be due to an interaction with the polystyrene growth substratum.Analysis of the biofilm communities grown in the presence of LCDMs by denaturing gradient gel electrophoresis (DGGE), showed no clear differences in community profiles depending on the LCDMs. However those biofilms grown in the light appeared to have a greater proportion of Alphaproteobacteria than those grown in the dark.Further study is needed to determine the level of fouling and the applicability of LCDMs in real micro-sensor systems. However, this study has shown that LCDMs have the potential to remediate, at least in part, the biofouling of marine micro-sensors

    Biofouling and its control for in situ lab-on-a-chip marine environmental sensors

    No full text
    Biofouling is the process by which biological organisms attach to surfaces in an aqueous environment. This occurs on nearly all surfaces in all natural aquatic environments, and can cause problems with the functioning of scientific equipment exposed to the marine environment for extended periods. At the National Oceanographic Centre in Southampton (NOCS), the Centre for Marine Microsystems (CMM) is developing lab-on-chip micro-sensors to monitor the chemical and biological environment in situ in the oceans. Due to the long periods (up to several months) that these sensors will be deployed, biofouling by microbial biofilms is an important concern for the efficient running of these sensors. The aim of this project was therefore to determine the potential level of fouling within the sensors and to investigate the potential use of low-concentration diffusible molecules (LCDMs) to remediate biofouling.Many of the sensors in development by CMM are designed to sense specific chemical species and they use various chemical reagents to achieve this. The effects of some of these reagents on the formation of biofilms by mixed marine communities were investigated. It was shown that Griess reagent and ortho-phthadialdehyde (OPA), used to sense nitrites and ammonium respectively, effectively stop biofilm formation by killing microorganisms before they can attach to surfaces.Biofouling on two different polymers, cyclic olefin copolymer (COC) and poly (methyl methacrylate) (PMMA), used in the construction of micro-sensors, was compared with biofouling on glass. No differences were observed between COC and PMMA, however a small but significant difference in surface coverage was observed between glass and COC at the early stages of exposure to the marine environment. The lack of differences between the two polymers suggests that biofouling is not an important consideration when deciding whether to construct sensors from COC or PMMA. However, the larger degree of fouling on hydrophobic COC compared with hydrophilic glass indicates a potential use of surface modifications as an antifouling strategy.The effects on biofouling of the LCDMs nitric oxide (NO), cis-2-decenoic acid (CDA) and patulin, were investigated to evaluate their potential for anti-fouling in marine micro sensors. All three molecules were shown to reduce the formation of biofilms by mixed marine communities, but colony counts suggested that the effect of patulin was due to toxicity as opposed to a physiological effect. Investigation of biofilm growth in the light and the dark revealed that there was less biofilm formation in the light that the dark and this effect was determined to be due to an interaction with the polystyrene growth substratum.Analysis of the biofilm communities grown in the presence of LCDMs by denaturing gradient gel electrophoresis (DGGE), showed no clear differences in community profiles depending on the LCDMs. However those biofilms grown in the light appeared to have a greater proportion of Alphaproteobacteria than those grown in the dark.Further study is needed to determine the level of fouling and the applicability of LCDMs in real micro-sensor systems. However, this study has shown that LCDMs have the potential to remediate, at least in part, the biofouling of marine micro-sensors

    NASA Tech Briefs, April 2012

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    Topics include: Computational Ghost Imaging for Remote Sensing; Digital Architecture for a Trace Gas Sensor Platform; Dispersed Fringe Sensing Analysis - DFSA; Indium Tin Oxide Resistor-Based Nitric Oxide Microsensors; Gas Composition Sensing Using Carbon Nanotube Arrays; Sensor for Boundary Shear Stress in Fluid Flow; Model-Based Method for Sensor Validation; Qualification of Engineering Camera for Long-Duration Deep Space Missions; Remotely Powered Reconfigurable Receiver for Extreme Environment Sensing Platforms; Bump Bonding Using Metal-Coated Carbon Nanotubes; In Situ Mosaic Brightness Correction; Simplex GPS and InSAR Inversion Software; Virtual Machine Language 2.1; Multi-Scale Three-Dimensional Variational Data Assimilation System for Coastal Ocean Prediction; Pandora Operation and Analysis Software; Fabrication of a Cryogenic Bias Filter for Ultrasensitive Focal Plane; Processing of Nanosensors Using a Sacrificial Template Approach; High-Temperature Shape Memory Polymers; Modular Flooring System; Non-Toxic, Low-Freezing, Drop-In Replacement Heat Transfer Fluids; Materials That Enhance Efficiency and Radiation Resistance of Solar Cells; Low-Cost, Rugged High-Vacuum System; Static Gas-Charging Plug; Floating Oil-Spill Containment Device; Stemless Ball Valve; Improving Balance Function Using Low Levels of Electrical Stimulation of the Balance Organs; Oxygen-Methane Thruster; Lunar Navigation Determination System - LaNDS; Launch Method for Kites in Low-Wind or No-Wind Conditions; Supercritical CO2 Cleaning System for Planetary Protection and Contamination Control Applications; Design and Performance of a Wideband Radio Telescope; Finite Element Models for Electron Beam Freeform Fabrication Process Autonomous Information Unit for Fine-Grain Data Access Control and Information Protection in a Net-Centric System; Vehicle Detection for RCTA/ANS (Autonomous Navigation System); Image Mapping and Visual Attention on the Sensory Ego-Sphere; HyDE Framework for Stochastic and Hybrid Model-Based Diagnosis; and IMAGESEER - IMAGEs for Education and Research

    Characterization of an enzymatic packed-bed microreactor: Experiments and modeling

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    A micro packed-bed reactor (µPBR) based on two-parallel-plates configuration with immobilized Candida antarctica lipase B in the form of porous particles (Novozym® 435) was theoretically and experimentally characterized. A residence time distribution (RTD) within µPBRs comprising various random distributions of particles placed in one layer was computationally predicted by a mesoscopic lattice Boltzmann (LB) method. Numerical simulations were compared with measurements of RTD, obtained by stimulus-response experiment with a pulse input using glucose as a tracer, monitored by an electrochemical glucose oxidase microbiosensor integrated with the reactor. The model was validated by a good agreement between the experimental data and predictions of LB model at different conditions. The developed µPBR was scaled-up in length and width comprising either a single or two layers of Novozym® 435 particles and compared regarding the selected enzyme-catalyzed transesterification. A linear increase in the productivity with the increase in all dimensions of the µPBR between two-plates demonstrated very efficient and simple approach for the capacity rise. Further characterization of µPBRs of various sizes using the piezoresistive pressure sensor revealed very low pressure drops as compared to their conventional counterparts and thereby great applicability for production systems based on numbering-up approach

    Sensor technologies for quality control in engineered tissue manufacturing

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    The use of engineered cells, tissues, and organs has the opportunity to change the way injuries and diseases are treated. Commercialization of these groundbreaking technologies has been limited in part by the complex and costly nature of their manufacture. Process-related variability and even small changes in the manufacturing process of a living product will impact its quality. Without real-time integrated detection, the magnitude and mechanism of that impact are largely unknown. Real-time and non-destructive sensor technologies are key for in-process insight and ensuring a consistent product throughout commercial scale-up and/or scale-out. The application of a measurement technology into a manufacturing process requires cell and tissue developers to understand the best way to apply a sensor to their process, and for sensor manufacturers to understand the design requirements and end-user needs. Furthermore, sensors to monitor component cells’ health and phenotype need to be compatible with novel integrated and automated manufacturing equipment. This review summarizes commercially relevant sensor technologies that can detect meaningful quality attributes during the manufacturing of regenerative medicine products, the gaps within each technology, and sensor considerations for manufacturing

    Molecular hydrogen and catalytic combustion in the production of hyperpolarized 83Kr and 129Xe MRI contrast agents

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    Hyperpolarized (hp) 83Kr is a promising MRI contrast agent for the diagnosis of pulmonary diseases affecting the surface of the respiratory zone. However, the distinct physical properties of 83Kr that enable unique MRI contrast also complicate the production of hp 83Kr. This work presents a radically new approach in the generation of hp 83Kr that can likewise be utilized for the production of hp 129Xe. Molecular nitrogen, typically used as buffer gas in spin exchange optical pumping (SEOP), was replaced by molecular hydrogen without penalty for the achievable hyperpolarization. In this particular study, the highest obtained nuclear spin polarizations were P = 29 % for 83Kr and P = 63 % for 129Xe. The results were reproduced over many SEOP cycles despite the laser induced on-resonance formation of rubidium hydride (RbH). Following SEOP, the H2 was reactively removed via catalytic combustion without measurable losses in hyperpolarized spin state of either 83Kr or 129Xe. Highly spin polarized 83Kr can now be purified for the first time to provide high signal intensity for the advancement of in vivo hp 83Kr MRI. More generally, a chemical reaction appears as a viable alternative to the cryogenic separation process, the primary purification method of hp 129Xe for the past 2 . decades. The inherent simplicity of the combustion process will facilitate hp 129Xe production and should allow for on-demand continuous flow of purified and highly spin polarized 129Xe

    The importance of microgradients for marine calcifiers

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    This thesis describes the importance of microgradients around organic tissue of calcifiers in comparison to bulk seawater conditions. It is shown that microenvironmental acidification around calcifiers can result from various causes, such as calcification (Chapter 1), microbial mat or sediment exposure (Chapter 2), respiration (Chapter 2) or ocean acidification conditions (Chapter 3). Some calcifiers, like sediment dwelling foraminifera and bivalves, are naturally adapted to low pH and hypoxic conditions (Chapter 1, 2). Yet, microenvironmental low pH, hypoxia and high levels of sulphide resulted in tissue necrosis of corals, but only if those conditions were trapped close to the tissue of corals for extended periods of time (Chapter 2). The performance of some calcifiers under low pH and hypoxic conditions thus depends upon the duration of the exposure, as well as the diffusional resistance between the bulk seawater and their tissues. If diffusivity around their organic tissues is significantly reduced, some calcifiers may not be able to maintain pH homeostasis and thus severely suffer from extended exposure to low pH, hypoxia and high levels of sulphide (Chapter 2)

    Investigation of hypothermic machine perfusion of human donor livers for improved organ preservation: measurements of organ quality and safety

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    Introduction Hypothermic machine perfusion (HMP) could improve the outcome of marginal liver transplantation, but the optimal perfusion setup and injury markers are unknown. The null hypothesis herein is no difference in cellular and mitochondrial injury during ex-situ preservation when comparing SCS and end-ischaemic HMP. Methods This is a single-centre, randomised study of end-ischaemic HMP in discarded human livers. A total of 45 livers were preserved for 4 hours with static cold storage (n = 7), arterial perfusion (n = 10), non-oxygen supplemented venous perfusion (n = 17), and oxygen supplemented venous perfusion (n = 11). Dynamic, biochemical, morphological, and mitochondrial parameters were analysed. Additionally, oxygenation kinetics and steatosis assessment were examined. Results Arterial perfusion resulted in higher resistance and lower flow compared with venous perfusion (p ≤ 0.01), as well as higher perfusate transaminases in the former group (p > 0.05). High-risk marginal livers were associated with 2-fold higher perfusate transaminases (p > 0.05) and higher post-preservation mitochondrial complex II-III activity (p = 0.01) compared to low-risk livers. Morphology and mitochondrial function were maintained in all groups and oxygenation did not trigger oxidative injury. Parenchymal oxygen measurement indicated evidence of oxygen consumption. A revised steatosis grading system using digital image analysis was accurate and showed high agreement with standard H&E assessment. Conclusion There was not enough evidence to reject the null hypothesis. Arterial-only perfusion might be inadequate for liver preservation based on the limited perfusate supply, but randomised trials are needed to determine the requirement of arterial perfusion in dual-vessel perfusion machines. The sensitivity of highrisk livers to ischaemia reperfusion injury might be reflected in their mitochondrial function, which needs to be assessed in future. Perfusate oxygenation is safe but the optimal perfusate oxygen remains unknown. DIA is a promising method, which can standardise steatosis evaluation
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