Novel Vegetation Indices to Identify Broccoli Plants Infected With Xanthomonas campestris pv. campestris

A rapid diagnosis of black rot in brassicas, a devastating disease caused by Xanthomonas campestris pv. campestris (Xcc), would be desirable to avoid significant crop yield losses. The main aim of this work was to develop a method of detection of Xcc infection on broccoli leaves. Such method is based on the use of imaging sensors that capture information about the optical properties of leaves and provide data that can be implemented on machine learning algorithms capable of learning patterns. Based on this knowledge, the algorithms are able to classify plants into categories (healthy and infected). To ensure the robustness of the detection method upon future alterations in climate conditions, the response of broccoli plants to Xcc infection was analyzed under a range of growing environments, taking current climate conditions as reference. Two projections for years 2081–2100 were selected, according to the Assessment Report of Intergovernmental Panel on Climate Change. Thus, the response of broccoli plants to Xcc infection and climate conditions has been monitored using leaf temperature and five conventional vegetation indices (VIs) derived from hyperspectral reflectance. In addition, three novel VIs, named diseased broccoli indices (DBI1-DBI3), were defined based on the spectral reflectance signature of broccoli leaves upon Xcc infection. Finally, the nine parameters were implemented on several classifying algorithms. The detection method offering the best performance of classification was a multilayer perceptron-based artificial neural network. This model identified infected plants with accuracies of 88.1, 76.9, and 83.3%, depending on the growing conditions. In this model, the three Vis described in this work proved to be very informative parameters for the disease detection. To our best knowledge, this is the first time that future climate conditions have been taken into account to develop a robust detection model using classifying algorithms.MCIN/AEI RTI2018-094652-B-I00ERDF: A way of making EuropeConsejo Superior de Investigaciones Cientificas (CSIC) through the Unidad de Recursos de Informacion Cientifica para la Investigacion (URICI

Development of an Optical System Based on Spectral Imaging Used for a Slug Control Robot

The state-of-the-art technique to control slug pests in agriculture is the spreading of slug pellets. This method has some downsides, because slug pellets also harm beneficials and often fail because their efficiency depends on the prevailing weather conditions. This study is part of a research project which is developing a pest control robot to monitor the field, detect slugs, and eliminate them. Robots represent a promising alternative to slug pellets. They work independent of weather conditions and can distinguish between pests and beneficials. As a prerequisite, a robot must be able to reliably identify slugs irrespective of the characteristics of the surrounding conditions. In this context, the utilization of computer vision and image analysis methods are challenging, because slugs look very similar to the soil, particularly in color images. Therefore, the goal of this study was to develop an optical filter-based system that distinguishes between slugs and soil. In this context, the spectral characteristics of both slugs and soil in the visible and visible near-infrared (VNIR) wavebands were measured. Conspicuous maxima followed by conspicuous local minima were found for the reflection spectra of slugs in the near infrared range from 850 nm to 990 nm]. Thus, this enabled differentiation between slugs and soils; soils showed a monotonic increase in the intensity of the relative reflection for this wavelength. The extrema determined in the reflection spectra of slugs were used to develop and set up a slug detector device consisting of a monochromatic camera, a filter changer and two narrow bandpass filters with nominal wavelengths of 925 nm and 975 nm. The developed optical system takes two photographs of the target area at night. By subtracting the pixel values of the images, the slugs are highlighted, and the soil is removed in the image due to the properties of the reflection spectra of soils and slugs. In the resulting image, the pixels of slugs were, on average, 12.4 times brighter than pixels of soil. This enabled the detection of slugs by a threshold method.Peer Reviewe

A Review on Advances in Automated Plant Disease Detection

Plant diseases cause major yield and economic losses. To detect plant disease at early stages, selecting appropriate techniques is imperative as it affects the cost, diagnosis time, and accuracy. This research gives a comprehensive review of various plant disease detection methods based on the images used and processing algorithms applied. It systematically analyzes various traditional machine learning and deep learning algorithms used for processing visible and spectral range images, and comparatively evaluates the work done in literature in terms of datasets used, various image processing techniques employed, models utilized, and efficiency achieved. The study discusses the benefits and restrictions of each method along with the challenges to be addressed for rapid and accurate plant disease detection. Results show that for plant disease detection, deep learning outperforms traditional machine learning algorithms while visible range images are more widely used compared to spectral images

Sensing and Automation Technologies for Ornamental Nursery Crop Production: Current Status and Future Prospects

The ornamental crop industry is an important contributor to the economy in the United States. The industry has been facing challenges due to continuously increasing labor and agricultural input costs. Sensing and automation technologies have been introduced to reduce labor requirements and to ensure efficient management operations. This article reviews current sensing and automation technologies used for ornamental nursery crop production and highlights prospective technologies that can be applied for future applications. Applications of sensors, computer vision, artificial intelligence (AI), machine learning (ML), Internet-of-Things (IoT), and robotic technologies are reviewed. Some advanced technologies, including 3D cameras, enhanced deep learning models, edge computing, radio-frequency identification (RFID), and integrated robotics used for other cropping systems, are also discussed as potential prospects. This review concludes that advanced sensing, AI and robotic technologies are critically needed for the nursery crop industry. Adapting these current and future innovative technologies will benefit growers working towards sustainable ornamental nursery crop production

IPM2.0: PRECISION AGRICULTURE FOR SMALL-SCALE CROP PRODUCTION

In order to manage pests impacting New England crop production integrated pest management (IPM) practices should be reevaluated or updated regularly to ensure that effective control of crop pests is being achieved. Three fungal taxa, Colletotrichum gloeosporioides, C. acutatum, and Glomerella cingulata, are currently associated with bitter-rot of apple (Malus domestica), with C. acutatum typically being the dominant species found in the northeastern United States. However, a recent phylogenetic study demonstrated that both C. gloeosporioides and C. acutatum are species complexes with over 10 distinct species being recovered from apple between the two studies. Based on this recent information, the objectives of this study were 1) to complete a phylogenetic analysis to determine species diversity and distribution of Colletotrichum isolates associated with bitter-rot and Glomerella leaf spot in the northeastern United States and 2) to evaluate the sensitivity of these isolates to several commercially used fungicides. A multi-gene phylogenetic analysis was completed using ITS, GADPH and BT gene sequences in order to determine which species and how many species of Colletotrichum were infecting apples in the northeastern U.S. The results of this study demonstrated that C. fioriniae is the primary pathogen causing both bitter rot and Glomerella leaf spot in the northeastern U.S. A second experiment was conducted in order to update management practices for apple scab, caused by the ascomycete Venturia inaequalis. The objective of this project was to evaluate the ability of RIMpro, an apple scab warning system, to control apple scab in New England apple orchards in addition to evaluating the performance of potassium bicarbonate + sulfur as a low-cost alternative spray material for the control of apple scab suitable for organic apple production. Use of RIMpro allowed for the reduction in the total number of spray applications made during the primary scab season by two sprays in 2013 and one spray in 2014 (28% and 25% reductions, respectively). Also, the potassium bicarbonate + sulfur treatment was shown to provide the same level of control as Captan. Finally, disease outbreaks, insect infestation, nutrient deficiencies, and weather variation constantly threaten to diminish annual yields and profits in orchard crop production systems. Automated crop inspection with an unmanned aerial vehicle (UAV) can allow growers to regularly survey crops and detect areas affected by disease or stress and lead to more efficient targeted applications of pesticides, water and fertilizer. The overall goal of this project was to develop a low cost aerial imaging platform coupling imaging sensors with UAVs to be used for monitoring crop health. Following completion of this research, we have identified a useful tool for agricultural and ecological applications

Mango anthracnose disease: the current situation and direction for future research

Mango anthracnose disease (MAD) is a destructive disease of mangoes, with estimated yield losses of up to 100% in unmanaged plantations. Several strains that constitute Colletotrichum complexes are implicated in MAD worldwide. All mangoes grown for commercial purposes are susceptible, and a resistant cultivar for all strains is not presently available on the market. The infection can widely spread before being detected since the disease is invincible until after a protracted latent period. The detection of multiple strains of the pathogen in Mexico, Brazil, and China has prompted a significant increase in research on the disease. Synthetic pesticide application is the primary management technique used to manage the disease. However, newly observed declines in anthracnose susceptibility to many fungicides highlight the need for more environmentally friendly approaches. Recent progress in understanding the host range, molecular and phenotypic characterization, and susceptibility of the disease in several mango cultivars is discussed in this review. It provides updates on the mode of transmission, infection biology and contemporary management strategies. We suggest an integrated and ecologically sound approach to managing MAD

Terahertz sensing analysis for early detection of ganoderma boninense disease using near infrared (NIR) spectrometer

Ganoderma boninense (G. boninense) infection reduces the productivity of oil palms and causes a significant impact on the economic viability of oil palm plantations. Early detection is critical for the effective management of this disease since there is no effective treatment that can stop the spread of this disease. The proposed system uses integrated hand-held near-infrared spectroscopy (NIRS) for early detection of G. boninense on asymptomatic oil palm seedlings and classification of spectral data using machine learning (ML) techniques. The non-destructive method using NIRS with ML and predictive analytics has the potential to be a highly sensitive and reliable method for the early detection of G. boninense. Spectral data are collected from 6 samples of inoculated and non-inoculated oil palm samples at nursery stages using an integrated NIRS sensor. Chemometrics is performed by implementing principal component analysis (PCA), derivatives and partial least square (PLS) regression to extract the vital information of the spectra. The significant wavelengths are at 1310 nm and 1450 nm which are attributable to ergosterol and water content, respectively. Furthermore, the SG derivatives spectra peaks corresponded to specific functional groups that could be utilized for the detection of G. boninense. These functional groups encompass the third overtone of N-H stretching, the second overtone of C-H stretching, and a combination band involving both C-H stretching and O-H stretching. High-performance liquid chromatography (HPLC) analysis is performed to identify the ergosterol content in oil palm sample. Ergosterol can be used as a biomarker for the detection of G. boninense since it can only be found in the fungal-infested plant. In classification, four different ML algorithms: K-Nearest Neighbour (kNN), Naïve Bayes (NB), Support Vector Machine (SVM) and Decision Tree (DT) are tested to classify healthy and infected oil palm samples. DT algorithm on leaves spectra achieves a satisfactory overall performance compared to the other classifiers with high accuracy up to 93.1% and an F1-score of 92.6%. Therefore, a DT-based predictive analytic on leaves NIR spectral reference data is developed for real-time detection of G. boninense infection. A portable smart G. boninense detection system prototype is developed by implementing the Internet of Things (IoT) into the system which enables the integration of sensors and server to perform prediction of healthy or infected oil palm seedlings. This working prototype showed that this proposed approach is reliable and practical for the early detection of G. boninense in oil palm seedlings

Pepper leaf disease recognition based on enhanced lightweight convolutional neural networks

Pepper leaf disease identification based on convolutional neural networks (CNNs) is one of the interesting research areas. However, most existing CNN-based pepper leaf disease detection models are suboptimal in terms of accuracy and computing performance. In particular, it is challenging to apply CNNs on embedded portable devices due to a large amount of computation and memory consumption for leaf disease recognition in large fields. Therefore, this paper introduces an enhanced lightweight model based on GoogLeNet architecture. The initial step involves compressing the Inception structure to reduce model parameters, leading to a remarkable enhancement in recognition speed. Furthermore, the network incorporates the spatial pyramid pooling structure to seamlessly integrate local and global features. Subsequently, the proposed improved model has been trained on the real dataset of 9183 images, containing 6 types of pepper diseases. The cross-validation results show that the model accuracy is 97.87%, which is 6% higher than that of GoogLeNet based on Inception-V1 and Inception-V3. The memory requirement of the model is only 10.3 MB, which is reduced by 52.31%-86.69%, comparing to GoogLeNet. We have also compared the model with the existing CNN-based models including AlexNet, ResNet-50 and MobileNet-V2. The result shows that the average inference time of the proposed model decreases by 61.49%, 41.78% and 23.81%, respectively. The results show that the proposed enhanced model can significantly improve performance in terms of accuracy and computing efficiency, which has potential to improve productivity in the pepper farming industry

Sustainable Agriculture and Advances of Remote Sensing (Volume 2)

Agriculture, as the main source of alimentation and the most important economic activity globally, is being affected by the impacts of climate change. To maintain and increase our global food system production, to reduce biodiversity loss and preserve our natural ecosystem, new practices and technologies are required. This book focuses on the latest advances in remote sensing technology and agricultural engineering leading to the sustainable agriculture practices. Earth observation data, in situ and proxy-remote sensing data are the main source of information for monitoring and analyzing agriculture activities. Particular attention is given to earth observation satellites and the Internet of Things for data collection, to multispectral and hyperspectral data analysis using machine learning and deep learning, to WebGIS and the Internet of Things for sharing and publication of the results, among others