DESIGN OF A GAIT ACQUISITION AND ANALYSIS SYSTEM FOR ASSESSING THE RECOVERY OF MICE POST-SPINAL CORD INJURY

Current methods of determining spinal cord recovery in mice, post-directed injury, are qualitative measures. This is due to the small size and quickness of mice. This thesis presents a design for a gait acquisition and analysis system able to capture the footfalls of a mouse, extract position and timing data, and report quantitative gait metrics to the operator. These metrics can then be used to evaluate the recovery of the mouse. This work presents the design evolution of the system, from initial sensor design concepts through prototyping and testing to the final implementation. The system utilizes a machine vision camera, a well-designed walkway enclosure, and image processing techniques to capture and analyze paw strikes. Quantitative results gained from live animal experiments are presented, and it is shown how the measurements can be used to determine healthy, injured, and recovered gait

Visible and Near Infrared imaging spectroscopy and the exploration of small scale hydrothermally altered and hydrated environments on Earth and Mars

The use of Visible and Near Infrared (VNIR) imaging spectroscopy is a cornerstone of planetary exploration. This work shall present an investigation into the limitations of scale, both spectral and spatial, in the utility of VNIR images for identifying small scale hydrothermal and potential hydrated environments on Mars, and regions of the Earth that can serve as martian analogues. Such settings represent possible habitable environments; important locations for astrobiological research. The ESA/Roscosmos ExoMars rover PanCam captures spectrally coarse but spatially high resolution VNIR images. This instrument is still in development and the first field trial of an emulator fitted with the final set of geological filters is presented here. Efficient image analysis techniques are explored and the ability to accurately characterise a hydrothermally altered region using PanCam data products is established. The CRISM orbital instrument has been returning hyperspectral VNIR images with an 18 m2 pixel resolution since 2006. The extraction of sub-pixel information from CRISM pixels using Spectral Mixture Analysis (SMA) algorithms is explored. Using synthetic datasets a full SMA pipeline consisting of publically available Matlab algorithms and optimised for investigation of mineralogically complex hydrothermal suites is developed for the first time. This is validated using data from Námafjall in Iceland, the region used to field trial the PanCam prototype. The pipeline is applied to CRISM images covering four regions on Mars identified as having potentially undergone hydrothermal alteration in their past. A second novel use of SMA to extract a unique spectral signature for the potentially hydrated Recurring Slope Lineae features on Mars is presented. The specific methodology presented shows promise and future improvements are suggested. The importance of combining different scales of data and recognising their limitations is discussed based on the results presented and ways in which to take the results presented in this thesis forward are given