In vivo human retinal and choroidal vasculature visualization using differential phase contrast swept source optical coherence tomography at 1060 nm

A differential phase contrast (DPC) method is validated for in vivo human retinal and choroidal vasculature visualization using high-speed swept-source optical coherence tomography (SS-OCT) at 1060 nm. The vasculature was identified as regions of motion by creating differential phase variance (DPV) tomograms: multiple B-scans were collected of individual slices through the retina and the variance of the phase differences was calculated. DPV captured the small vessels and the meshwork of capillaries associated with the inner retina in en face images over 4 mm^2 in a normal subject. En face DPV images were capable of capturing the microvasculature and regions of motion through the inner retina and choroid

The construction and operation of a water tunnel in application to flow visualization studies of an oscillating airfoil

The water tunnel which was constructed at the NASA Ames Research Center is described along with the flow field adjacent to an oscillating airfoil. The design and operational procedures of the tunnel are described in detail. Hydrogen bubble and thymol blue techniques are used to visualize the flow field. Results of the flow visualizations are presented in a series of still pictures and a high speed movie. These results show that time stall is more complicated than simple shedding from the leading edge or the trailing edge, particularly at relatively low frequency oscillations comparable to those of a helicopter blade. Therefore, any successful theory for predicting the stall loads on the helicopter blades must treat an irregular separated region rather than a discrete vortex passing over each blade surface

Visualization of leukocyte transendothelial and interstitial migration using reflected light oblique transillumination in intravital video microscopy

Dynamic visualization of the intravascular events leading to the extravasation of leukocytes into tissues by intravital microscopy has significantly expanded our understanding of the underlying molecular processes. In contrast, the detailed observation of leukocyte transendothelial and interstitial migration in vivo has been hampered by the poor image contrast of cells within turbid media that is obtainable by conventional brightfield microscopy. Here we present a microscopic method, termed reflected light oblique transillumination microscopy, that makes use of the optical interference phenomena generated by oblique transillumination to visualize subtle gradients of refractive indices within tissues for enhanced image contrast. Using the mouse cremaster muscle, we demonstrate that this technique makes possible the reliable quantification of extravasated leukocytes as well as the characterization of morphological phenomena of leukocyte transendothelial and interstitial migration

Laser Ultrasound Inspection Based on Wavelet Transform and Data Clustering for Defect Estimation in Metallic Samples

Laser-generated ultrasound is a modern non-destructive testing technique. It has been investigated over recent years as an alternative to classical ultrasonic methods, mainly in industrial maintenance and quality control procedures. In this study, the detection and reconstruction of internal defects in a metallic sample is performed by means of a time-frequency analysis of ultrasonic waves generated by a laser-induced thermal mechanism. In the proposed methodology, we used wavelet transform due to its multi-resolution time frequency characteristics. In order to isolate and estimate the corresponding time of flight of eventual ultrasonic echoes related to internal defects, a density-based spatial clustering was applied to the resulting time frequency maps. Using the laser scan beam’s position, the ultrasonic transducer’s location and the echoes’ arrival times were determined, the estimation of the defect’s position was carried out afterwards. Finally, clustering algorithms were applied to the resulting geometric solutions from the set of the laser scan points which was proposed to obtain a two-dimensional projection of the defect outline over the scan plane. The study demonstrates that the proposed method of wavelet transform ultrasonic imaging can be effectively applied to detect and size internal defects without any reference information, which represents a valuable outcome for various applications in the industry. View Full-TextPeer ReviewedPostprint (published version

In vivo laser Doppler holography of the human retina

The eye offers a unique opportunity for non-invasive exploration of cardiovascular diseases. Optical angiography in the retina requires sensitive measurements, which hinders conventional full-field laser Doppler imaging schemes. To overcome this limitation, we used digital holography to perform laser Doppler perfusion imaging of the human retina in vivo with near-infrared light. Wideband measurements of the beat frequency spectrum of optical interferograms recorded with a 39 kHz CMOS camera are analyzed by short-time Fourier transformation. Power Doppler images and movies drawn from the zeroth moment of the power spectrum density reveal blood flows in retinal and choroidal vessels over 512 $\times$ 512 pixels covering 2.4 $\times$ 2.4 mm$^2$ on the retina with a 13 ms temporal resolution.Comment: 5 pages, 5 figure

Visualizing Magnitude and Direction in Flow Fields

In weather visualizations, it is common to see vector data represented by glyphs placed on grids. The glyphs either do not encode magnitude in readable steps, or have designs that interfere with the data. The grids form strong but irrelevant patterns. Directional, quantitative glyphs bent along streamlines are more effective for visualizing flow patterns. With the goal of improving the perception of flow patterns in weather forecasts, we designed and evaluated two variations on a glyph commonly used to encode wind speed and direction in weather visualizations. We tested the ability of subjects to determine wind direction and speed: the results show the new designs are superior to the traditional. In a second study we designed and evaluated new methods for representing modeled wave data using similar streamline-based designs. We asked subjects to rate the marine weather visualizations: the results revealed a preference for some of the new designs

Shock-induced mixing of a light-gas cylinder

Experiments have been carried out to quantify the mixing induced by the interaction of a weak shock wave with a cylindrical volume of a gas (helium) that is lighter than its surroundings (air). In these experiments a round laminar jet was used to produce the light-gas cylinder, and planar laser-induced fluorescence (PLIF), utilizing a fluorescent tracer (biacetyl) mixed with the helium, was used to visualize the flow. These techniques provide a higher quality of flow visualization than that obtained in previous investigations. In addition, the PLIF technique could be used for the measurement of species concentration. The distortion of the helium cylinder produced by the passing shock wave was found to be similar to that displayed by images from previous experimental and computational investigations. The downstream displacement of several points on the boundary of the light-gas cylinder are measured and agree reasonably well with the results of earlier experimental and theoretical studies as well. Because the mixing process causes the helium originally contained within the cylinder to be dispersed into the surrounding air, the PLIF image area inside the contour at one half the maximum concentration of the fluorescent tracer decreases as the two gases mixed. The change in this area is used as a measure of the mixing rate, and it is found that the time rate of change of this area divided by the area of the initial jet is approximately - 0.7 X 10^3 S^(-1)