Bahasa Inggris Kelas X

Penyajian buku teks untuk disusun dengan tujuan agar peserta didik dapat melakukan proses pencarian pengetahuan berkenaan dengan materi pelajaran melalui berbagai aktivitas para ilmuwan dalam melakukan eksperimen, dengan demikian peserta didik diarahkan untuk menemukan sendiri berbagai fakta, membangun konsep, dan nilai-nilai baru secara mandir

Effects of Methyl Cycle Substrate Availability on Epigenetic Stability of Human Embryonic Stem Cells

A link has been hypothesised to occur between suboptimal maternal nutrition and impaired foetal development leading to a predisposition to a range of adult pathologies. As a clear connection between dietary intake of methyl group donors and epigenetic defects has been demonstrated both *in vivo* and *in vitro*, this project had the purpose of generating a disruption into the methyl/folate cycle to investigate DNA methylation alterations during human preimplantation embryo development, using human embryonic stem cells (hESCs) as an in vitro model. In particular, HUES-7 stem cells were employed and cultured using either standard or methyl deficient media to test this hypothesis. After the treatments, that included an inhibitor of a key enzyme of the cycle, Differentially Methylated Regions (DMRs) of six imprinted genes were analysed and assessed for their methylation status at Cytosine-phospho-Guanosine (CpG) sites. As a consistent decrease of methylation was observed for the gene *H19* in treated cultures, its allelic expression was then investigated and an initial process of Loss Of Imprinting (LOI) was found. Additionally, global DNA MethylTransferase (DNMT) activity was examined and a statistically significant decrease in treated samples was detected. Finally, hESCs were differentiated into Embryoid Bodies(hEBs), which were compared and stained for pluripotency and germ-layer specific markers. Consistently different expression of *OCT-4* and *NANOG* was noticed for treated-culture derived hEBs

Spartan Daily, May 2, 1996

Volume 106, Issue 62https://scholarworks.sjsu.edu/spartandaily/8847/thumbnail.jp

Spartan Daily, October 20, 1942

Volume 31, Issue 14https://scholarworks.sjsu.edu/spartandaily/3494/thumbnail.jp

Spartan Daily, November 28, 1978

Volume 71, Issue 58https://scholarworks.sjsu.edu/spartandaily/6417/thumbnail.jp

Spartan Daily, February 3, 1997

Volume 108, Issue 7https://scholarworks.sjsu.edu/spartandaily/9084/thumbnail.jp

Spartan Daily, May 7, 2003

Volume 120, Issue 66https://scholarworks.sjsu.edu/spartandaily/9862/thumbnail.jp

Spartan Daily, November 12, 1991

Volume 97, Issue 51https://scholarworks.sjsu.edu/spartandaily/8188/thumbnail.jp