2 research outputs found
DSD – An integrated, web-accessible database of Dehydrogenase Enzyme Stereospecificities
BACKGROUND: Dehydrogenase enzymes belong to the oxidoreductase class and utilise the coenzymes NAD and NADP. Stereo-selectivity is focused on the C4 hydrogen atoms of the nicotinamide ring of NAD(P). Depending upon which hydrogen is transferred at the C4 location, the enzyme is designated as A or B stereospecific. DESCRIPTION: The Dehydrogenase Stereospecificity Database v1.0 (DSD) provides a compilation of enzyme stereochemical data, as sourced from the primary literature, in the form of a web-accessible database. There are two search engines, a menu driven search and a BLAST search. The entries are also linked to several external databases, including the NCBI and the Protein Data Bank, providing wide background information. The database is freely available online at: CONCLUSION: DSD is a unique compilation available on-line for the first time which provides a key resource for the comparative analysis of reductase hydrogen transfer stereospecificity. As databases increasingly form the backbone of science, largely complete databases such as DSD, are a vital addition
Removing organic contamination from water, using UV radiation.
Diplomová práce se zabĂ˝vá moĹľnostmi vyuĹľitĂ UV zářenĂ pro odstranÄ›nĂ nežádoucĂch látek z vod. V teoretickĂ© části shrnuje zdroje UV zářenĂ pro vodárenskĂ© aplikace, jejich vĂ˝hody a specifika. Jsou zde popsány takzvanĂ© pokroÄŤilĂ© oxidaÄŤnĂ procesy vyuĹľĂvajĂcĂ právÄ› tento druh zářenĂ. Dále jsou rozvedeny degradaÄŤnĂ procesy skupiny polutantĹŻ pyridinu a jeho derivátĹŻ, konkrĂ©tnÄ› halogenovanĂ˝ch derivátĹŻ. V experimentálnà části je zkoumána fotodegradabilita a rychlost odstranÄ›nĂ pyridinu z modelovĂ© vody cirkulujĂcĂ v uzavĹ™enĂ©m systĂ©mu. Je hodnocen jak vliv koncentrace pyridinu v tĂ©to modelovĂ© vodÄ›, tak pĹŻsobenĂ dávky pĹ™idanĂ©ho peroxidu vodĂku. Ke stanovenĂ koncentracĂ pyridinu v odebranĂ˝ch vzorcĂch bylo vyuĹľito plynovĂ© chromatografie.This diploma thesis focuses on possibilities of applications of UV radiation to remove pollutants from water. It summarizes sources of UV radiation and list their benefits and properties. The thesis characterizes so called advanced oxidation processes using UV light. Degradation pathways od pyridine and its derivatives especially halogenated pyridines are described. The photodegradability of pyridine and a rate of this reaction in model water is investigated in the experimental part. Also, the effect of concentration and dose of H2O2 is assessed. Gas chromatography was used to determinate concentrations of pyridine in samples.