Relative and contextual contribution of different sources to the composition and abundance of indoor air bacteria in residences.

BackgroundThe study of the microbial communities in the built environment is of critical importance as humans spend the majority of their time indoors. While the microorganisms in living spaces, especially those in the air, can impact health and well-being, little is known of their identity and the processes that determine their assembly. We investigated the source-sink relationships of airborne bacteria in 29 homes in the San Francisco Bay Area. Samples taken in the sites expected to be source habitats for indoor air microbes were analyzed by 16S rRNA-based pyrosequencing and quantitative PCR. The community composition was related to the characteristics of the household collected at the time of sampling, including the number of residents and pets, activity levels, frequency of cooking and vacuum cleaning, extent of natural ventilation, and abundance and type of vegetation surrounding the building.ResultsIndoor air harbored a diverse bacterial community dominated by Diaphorobacter sp., Propionibacterium sp., Sphingomonas sp., and Alicyclobacillus sp. Source-sink analysis suggested that outdoor air was the primary source of indoor air microbes in most homes. Bacterial phylogenetic diversity and relative abundance in indoor air did not differ statistically from that in outdoor air. Moreover, the abundance of bacteria in outdoor air was positively correlated with that in indoor air, as would be expected if outdoor air was the main contributor to the bacterial community in indoor bioaerosols. The number of residents, presence of pets, and local tap water also influenced the diversity and size of indoor air microbes. The bacterial load in air increased with the number of residents, activity, and frequency of natural ventilation, and the proportion of bacteria putatively derived from skin increased with the number of residents. Vacuum cleaning increased the signature of pet- and floor-derived bacteria in indoor air, while the frequency of natural ventilation decreased the relative abundance of tap water-derived microorganisms in air.ConclusionsIndoor air in residences harbors a diverse bacterial community originating from both outdoor and indoor sources and is strongly influenced by household characteristics

Study and simulation of low rate video coding schemes

The semiannual report is included. Topics covered include communication, information science, data compression, remote sensing, color mapped images, robust coding scheme for packet video, recursively indexed differential pulse code modulation, image compression technique for use on token ring networks, and joint source/channel coder design

Design, development and application of an automated framework for cell growth and laboratory evolution

Precise control over microbial cell growth conditions could enable detection of minute phenotypic changes, which would improve our understanding of how genotypes are shaped by adaptive selection. Although automated cell- culture systems such as bioreactors offer strict control over liquid culture conditions, they often do not scale to high-throughput or require cumbersome redesign to alter growth conditions. I report the design and validation of eVOLVER, a scalable DIY framework that can be configured to carry out high- throughput growth experiments in molecular evolution, systems biology, and microbiology. I perform high-throughput evolution of yeast across systematically varied population density niches to show how eVOLVER can precisely characterize adaptive niches. I describe growth selection using time-varying temperature programs on a genome-wide yeast knockout library to identify strains with altered sensitivity to changes in temperature magnitude or frequency. Inspired by large-scale integration of electronics and microfluidics, I also demonstrate millifluidic multiplexing modules that enable multiplexed media routing, cleaning, vial-to-vial transfers and automated yeast mating

Linear Transmit-Receive Strategies for Multi-user MIMO Wireless Communications

Die Notwendigkeit zur Unterdrueckung von Interferenzen auf der einen Seite und zur Ausnutzung der durch Mehrfachzugriffsverfahren erzielbaren Gewinne auf der anderen Seite rueckte die raeumlichen Mehrfachzugriffsverfahren (Space Division Multiple Access, SDMA) in den Fokus der Forschung. Ein Vertreter der raeumlichen Mehrfachzugriffsverfahren, die lineare Vorkodierung, fand aufgrund steigender Anzahl an Nutzern und Antennen in heutigen und zukuenftigen Mobilkommunikationssystemen besondere Beachtung, da diese Verfahren das Design von Algorithmen zur Vorcodierung vereinfachen. Aus diesem Grund leistet diese Dissertation einen Beitrag zur Entwicklung linearer Sende- und Empfangstechniken fuer MIMO-Technologie mit mehreren Nutzern. Zunaechst stellen wir ein Framework zur Approximation des Datendurchsatzes in Broadcast-MIMO-Kanaelen mit mehreren Nutzern vor. In diesem Framework nehmen wir das lineare Vorkodierverfahren regularisierte Blockdiagonalisierung (RBD) an. Durch den Vergleich von Dirty Paper Coding (DPC) und linearen Vorkodieralgorithmen (z.B. Zero Forcing (ZF) und Blockdiagonalisierung (BD)) ist es uns moeglich, untere und obere Schranken fuer den Unterschied bezueglich Datenraten und bezueglich Leistung zwischen beiden anzugeben. Im Weiteren entwickeln wir einen Algorithmus fuer koordiniertes Beamforming (Coordinated Beamforming, CBF), dessen Loesung sich in geschlossener Form angeben laesst. Dieser CBF-Algorithmus basiert auf der SeDJoCo-Transformation und loest bisher vorhandene Probleme im Bereich CBF. Im Anschluss schlagen wir einen iterativen CBF-Algorithmus namens FlexCoBF (flexible coordinated beamforming) fuer MIMO-Broadcast-Kanaele mit mehreren Nutzern vor. Im Vergleich mit bis dato existierenden iterativen CBF-Algorithmen kann als vielversprechendster Vorteil die freie Wahl der linearen Sende- und Empfangsstrategie herausgestellt werden. Das heisst, jede existierende Methode der linearen Vorkodierung kann als Sendestrategie genutzt werden, waehrend die Strategie zum Empfangsbeamforming frei aus MRC oder MMSE gewaehlt werden darf. Im Hinblick auf Szenarien, in denen Mobilfunkzellen in Clustern zusammengefasst sind, erweitern wir FlexCoBF noch weiter. Hier wurde das Konzept der koordinierten Mehrpunktverbindung (Coordinated Multipoint (CoMP) transmission) integriert. Zuletzt stellen wir drei Moeglichkeiten vor, Kanalzustandsinformationen (Channel State Information, CSI) unter verschiedenen Kanalumstaenden zu erlangen. Die Qualitaet der Kanalzustandsinformationen hat einen starken Einfluss auf die Guete des Uebertragungssystems. Die durch unsere neuen Algorithmen erzielten Verbesserungen haben wir mittels numerischer Simulationen von Summenraten und Bitfehlerraten belegt.In order to combat interference and exploit large multiplexing gains of the multi-antenna systems, a particular interest in spatial division multiple access (SDMA) techniques has emerged. Linear precoding techniques, as one of the SDMA strategies, have obtained more attention due to the fact that an increasing number of users and antennas involved into the existing and future mobile communication systems requires a simplification of the precoding design. Therefore, this thesis contributes to the design of linear transmit and receive strategies for multi-user MIMO broadcast channels in a single cell and clustered multiple cells. First, we present a throughput approximation framework for multi-user MIMO broadcast channels employing regularized block diagonalization (RBD) linear precoding. Comparing dirty paper coding (DPC) and linear precoding algorithms (e.g., zero forcing (ZF) and block diagonalization (BD)), we further quantify lower and upper bounds of the rate and power offset between them as a function of the system parameters such as the number of users and antennas. Next, we develop a novel closed-form coordinated beamforming (CBF) algorithm (i.e., SeDJoCo based closed-form CBF) to solve the existing open problem of CBF. Our new algorithm can support a MIMO system with an arbitrary number of users and transmit antennas. Moreover, the application of our new algorithm is not only for CBF, but also for blind source separation (BSS), since the same mathematical model has been used in BSS application.Then, we further propose a new iterative CBF algorithm (i.e., flexible coordinated beamforming (FlexCoBF)) for multi-user MIMO broadcast channels. Compared to the existing iterative CBF algorithms, the most promising advantage of our new algorithm is that it provides freedom in the choice of the linear transmit and receive beamforming strategies, i.e., any existing linear precoding method can be chosen as the transmit strategy and the receive beamforming strategy can be flexibly chosen from MRC or MMSE receivers. Considering clustered multiple cell scenarios, we extend the FlexCoBF algorithm further and introduce the concept of the coordinated multipoint (CoMP) transmission. Finally, we present three strategies for channel state information (CSI) acquisition regarding various channel conditions and channel estimation strategies. The CSI knowledge is required at the base station in order to implement SDMA techniques. The quality of the obtained CSI heavily affects the system performance. The performance enhancement achieved by our new strategies has been demonstrated by numerical simulation results in terms of the system sum rate and the bit error rate

Cooperative Radio Communications for Green Smart Environments

The demand for mobile connectivity is continuously increasing, and by 2020 Mobile and Wireless Communications will serve not only very dense populations of mobile phones and nomadic computers, but also the expected multiplicity of devices and sensors located in machines, vehicles, health systems and city infrastructures. Future Mobile Networks are then faced with many new scenarios and use cases, which will load the networks with different data traffic patterns, in new or shared spectrum bands, creating new specific requirements. This book addresses both the techniques to model, analyse and optimise the radio links and transmission systems in such scenarios, together with the most advanced radio access, resource management and mobile networking technologies. This text summarises the work performed by more than 500 researchers from more than 120 institutions in Europe, America and Asia, from both academia and industries, within the framework of the COST IC1004 Action on "Cooperative Radio Communications for Green and Smart Environments". The book will have appeal to graduates and researchers in the Radio Communications area, and also to engineers working in the Wireless industry. Topics discussed in this book include: • Radio waves propagation phenomena in diverse urban, indoor, vehicular and body environments• Measurements, characterization, and modelling of radio channels beyond 4G networks• Key issues in Vehicle (V2X) communication• Wireless Body Area Networks, including specific Radio Channel Models for WBANs• Energy efficiency and resource management enhancements in Radio Access Networks• Definitions and models for the virtualised and cloud RAN architectures• Advances on feasible indoor localization and tracking techniques• Recent findings and innovations in antenna systems for communications• Physical Layer Network Coding for next generation wireless systems• Methods and techniques for MIMO Over the Air (OTA) testin

Systems and Methods for Measuring and Improving End-User Application Performance on Mobile Devices

In today's rapidly growing smartphone society, the time users are spending on their smartphones is continuing to grow and mobile applications are becoming the primary medium for providing services and content to users. With such fast paced growth in smart-phone usage, cellular carriers and internet service providers continuously upgrade their infrastructure to the latest technologies and expand their capacities to improve the performance and reliability of their network and to satisfy exploding user demand for mobile data. On the other side of the spectrum, content providers and e-commerce companies adopt the latest protocols and techniques to provide smooth and feature-rich user experiences on their applications. To ensure a good quality of experience, monitoring how applications perform on users' devices is necessary. Often, network and content providers lack such visibility into the end-user application performance. In this dissertation, we demonstrate that having visibility into the end-user perceived performance, through system design for efficient and coordinated active and passive measurements of end-user application and network performance, is crucial for detecting, diagnosing, and addressing performance problems on mobile devices. My dissertation consists of three projects to support this statement. First, to provide such continuous monitoring on smartphones with constrained resources that operate in such a highly dynamic mobile environment, we devise efficient, adaptive, and coordinated systems, as a platform, for active and passive measurements of end-user performance. Second, using this platform and other passive data collection techniques, we conduct an in-depth user trial of mobile multipath to understand how Multipath TCP (MPTCP) performs in practice. Our measurement study reveals several limitations of MPTCP. Based on the insights gained from our measurement study, we propose two different schemes to address the identified limitations of MPTCP. Last, we show how to provide visibility into the end- user application performance for internet providers and in particular home WiFi routers by passively monitoring users' traffic and utilizing per-app models mapping various network quality of service (QoS) metrics to the application performance.PHDComputer Science & EngineeringUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttps://deepblue.lib.umich.edu/bitstream/2027.42/146014/1/ashnik_1.pd