Background-deflection Brillouin microscopy reveals altered biomechanics of intracellular stress granules by ALS protein FUS

Altered cellular biomechanics have been implicated as key photogenic triggers in age-related diseases. An aberrant liquid-to-solid phase transition, observed in in vitro reconstituted droplets of FUS protein, has been recently proposed as a possible pathogenic mechanism for amyotrophic lateral sclerosis (ALS). Whether such transition occurs in cell environments is currently unknown as a consequence of the limited measuring capability of the existing techniques, which are invasive or lack of subcellular resolution. Here we developed a non-contact and label-free imaging method, named background-deflection Brillouin microscopy, to investigate the three-dimensional intracellular biomechanics at a sub-micron resolution. Our method exploits diffraction to achieve an unprecedented 10,000-fold enhancement in the spectral contrast of single-stage spectrometers, enabling, to the best of our knowledge, the first direct biomechanical analysis on intracellular stress granules containing ALS mutant FUS protein in fixed cells. Our findings provide fundamental insights on the critical aggregation step underlying the neurodegenerative ALS disease

Observing many body effects on lepton pair production from low mass enhancement and flow at RHIC and LHC energies

The $\rho$ spectral function at finite temperature calculated using the real-time formalism of thermal field theory is used to evaluate the low mass dilepton spectra. The analytic structure of the $\rho$ propagator is studied and contributions to the dilepton yield in the region below the bare $\rho$ peak from the different cuts in the spectral function are discussed. The space-time integrated yield shows significant enhancement in the region below the bare $\rho$ peak in the invariant mass spectra. It is argued that the variation of the inverse slope of the transverse mass ($M_T$) distribution can be used as an efficient tool to predict the presence of two different phases of the matter during the evolution of the system. Sensitivity of the effective temperature obtained from the slopes of the $M_T$ spectra to the medium effects are studied

High-fidelity single-shot readout for a spin qubit via an enhanced latching mechanism

The readout of semiconductor spin qubits based on spin blockade is fast but suffers from a small charge signal. Previous work suggested large benefits from additional charge mapping processes, however uncertainties remain about the underlying mechanisms and achievable fidelity. In this work, we study the single-shot fidelity and limiting mechanisms for two variations of an enhanced latching readout. We achieve average single-shot readout fidelities > 99.3% and > 99.86% for the conventional and enhanced readout respectively, the latter being the highest to date for spin blockade. The signal amplitude is enhanced to a full one-electron signal while preserving the readout speed. Furthermore, layout constraints are relaxed because the charge sensor signal is no longer dependent on being aligned with the conventional (2, 0) - (1, 1) charge dipole. Silicon donor-quantum-dot qubits are used for this study, for which the dipole insensitivity substantially relaxes donor placement requirements. One of the readout variations also benefits from a parametric lifetime enhancement by replacing the spin-relaxation process with a charge-metastable one. This provides opportunities to further increase the fidelity. The relaxation mechanisms in the different regimes are investigated. This work demonstrates a readout that is fast, has one-electron signal and results in higher fidelity. It further predicts that going beyond 99.9% fidelity in a few microseconds of measurement time is within reach.Comment: Supplementary information is included with the pape

Evolution of Structure and Superconductivity in Ba(Ni1−x_{1-x}Cox_x)2_2As2_2

The effects of Co-substitution on Ba(Ni$_{1-x}$Co$_x$)$_2$As$_2$ ($0\leq x\leq 0.251$) single crystals grown out of Pb flux are investigated via transport, magnetic, and thermodynamic measurements. BaNi$_2$As$_2$ exhibits a first order tetragonal to triclinic structural phase transition at $T_s=137 K$ upon cooling, and enters a superconducting phase below $T_c=0.7 K$. The structural phase transition is sensitive to cobalt content and is suppressed completely by $x\geq0.133$. The superconducting critical temperature, $T_c$, increases continuously with $x$, reaching a maximum of $T_c=2.3 K$ at the structural critical point $x=0.083$ and then decreases monotonically until superconductivity is no longer observable well into the tetragonal phase. In contrast to similar BaNi$_2$As$_2$ substitutional studies, which show an abrupt change in $T_c$ at the triclinic-tetragonal boundary that extends far into the tetragonal phase, Ba(Ni$_{1-x}$Co$_x$)$_2$As$_2$ exhibits a dome-like phase diagram centered around the first-order critical point. Together with an anomalously large heat capacity jump $\Delta C_e/\gamma T\sim 2.2$ at optimal doping, the smooth evolution of $T_c$ in the Ba(Ni$_{1-x}$Co$_x$)$_2$As$_2$ system suggests a mechanism for pairing enhancement other than phonon softening.Comment: 7 pages, 8 figure

Control of Optical Dynamic Memory Capacity of an Atomic Bose-Einstein Condensate

Light storage in an atomic Bose-Einstein condensate is one of the most practical usage of these coherent atom-optical systems. In order to make them even more practical, it is necessary to enhance our ability to inject multiple pulses into the condensate. In this paper, we report that dispersion of pulses injected into the condensate can be compensated by optical nonlinearity. In addition, we will present a brief review of our earlier results in which enhancement of light storage capacity is accomplished by utilizing multi-mode light propagation or choosing an optimal set of experimental parameters.Comment: 4 figures, 11 page