Pollen segmentation and feature evaluation for automatic classification in bright-field microscopy

14 págs.; 10 figs.; 7 tabs.; 1 app.© 2014 Elsevier B.V. Besides the well-established healthy properties of pollen, palynology and apiculture are of extreme importance to avoid hard and fast unbalances in our ecosystems. To support such disciplines computer vision comes to alleviate tedious recognition tasks. In this paper we present an applied study of the state of the art in pattern recognition techniques to describe, analyze, and classify pollen grains in an extensive dataset specifically collected (15 types, 120 samples/type). We also propose a novel contour-inner segmentation of grains, improving 50% of accuracy. In addition to published morphological, statistical, and textural descriptors, we introduce a new descriptor to measure the grain's contour profile and a logGabor implementation not tested before for this purpose. We found a significant improvement for certain combinations of descriptors, providing an overall accuracy above 99%. Finally, some palynological features that are still difficult to be integrated in computer systems are discussed.This work has been supported by the European project APIFRESH FP7-SME-2008-2 ‘‘Developing European standards for bee pollen and royal jelly: quality, safety and authenticity’’ and we would like to thank to Mr. Walter Haefeker, President of the European Professional Beekeepers Association (EPBA). J. Victor Marcos is a ‘‘Juan de la Cierva’’ research fellow funded by the Spanish Ministry of Economy and Competitiveness. Rodrigo Nava thanks Consejo Nacional de Ciencia y Tecnología (CONACYT) and PAPIIT Grant IG100814.Peer Reviewe

Differentiation between Pancreatic Ductal Adenocarcinoma and Normal Pancreatic Tissue for Treatment Response Assessment using Multi-Scale Texture Analysis of CT Images

Background: Pancreatic ductal adenocarcinoma (PDAC) is the most prevalent type of pancreas cancer with a high mortality rate and its staging is highly dependent on the extent of involvement between the tumor and surrounding vessels, facilitating treatment response assessment in PDAC. Objective: This study aims at detecting and visualizing the tumor region and the surrounding vessels in PDAC CT scan since, despite the tumors in other abdominal organs, clear detection of PDAC is highly difficult. Material and Methods: This retrospective study consists of three stages: 1) a patch-based algorithm for differentiation between tumor region and healthy tissue using multi-scale texture analysis along with L1-SVM (Support Vector Machine) classifier, 2) a voting-based approach, developed on a standard logistic function, to mitigate false detections, and 3) 3D visualization of the tumor and the surrounding vessels using ITK-SNAP software. Results: The results demonstrate that multi-scale texture analysis strikes a balance between recall and precision in tumor and healthy tissue differentiation with an overall accuracy of 0.78±0.12 and a sensitivity of 0.90±0.09 in PDAC. Conclusion: Multi-scale texture analysis using statistical and wavelet-based features along with L1-SVM can be employed to differentiate between healthy and pancreatic tissues. Besides, 3D visualization of the tumor region and surrounding vessels can facilitate the assessment of treatment response in PDAC. However, the 3D visualization software must be further developed for integrating with clinical applications

Biometric iris image segmentation and feature extraction for iris recognition

PhD ThesisThe continued threat to security in our interconnected world today begs for urgent solution. Iris biometric like many other biometric systems provides an alternative solution to this lingering problem. Although, iris recognition have been extensively studied, it is nevertheless, not a fully solved problem which is the factor inhibiting its implementation in real world situations today. There exists three main problems facing the existing iris recognition systems: 1) lack of robustness of the algorithm to handle non-ideal iris images, 2) slow speed of the algorithm and 3) the applicability to the existing systems in real world situation. In this thesis, six novel approaches were derived and implemented to address these current limitation of existing iris recognition systems. A novel fast and accurate segmentation approach based on the combination of graph-cut optimization and active contour model is proposed to define the irregular boundaries of the iris in a hierarchical 2-level approach. In the first hierarchy, the approximate boundary of the pupil/iris is estimated using a method based on Hough’s transform for the pupil and adapted starburst algorithm for the iris. Subsequently, in the second hierarchy, the final irregular boundary of the pupil/iris is refined and segmented using graph-cut based active contour (GCBAC) model proposed in this work. The segmentation is performed in two levels, whereby the pupil is segmented first before the iris. In order to detect and eliminate noise and reflection artefacts which might introduce errors to the algorithm, a preprocessing technique based on adaptive weighted edge detection and high-pass filtering is used to detect reflections on the high intensity areas of the image while exemplar based image inpainting is used to eliminate the reflections. After the segmentation of the iris boundaries, a post-processing operation based on combination of block classification method and statistical prediction approach is used to detect any super-imposed occluding eyelashes/eyeshadows. The normalization of the iris image is achieved though the rubber sheet model. In the second stage, an approach based on construction of complex wavelet filters and rotation of the filters to the direction of the principal texture direction is used for the extraction of important iris information while a modified particle swam optimization (PSO) is used to select the most prominent iris features for iris encoding. Classification of the iriscode is performed using adaptive support vector machines (ASVM). Experimental results demonstrate that the proposed approach achieves accuracy of 98.99% and is computationally about 2 times faster than the best existing approach.Ebonyi State University and Education Task Fund, Nigeri

Elliptical Monogenic Wavelets for the analysis and processing of color images

International audienceThis paper studies and gives new algorithms for image processing based on monogenic wavelets. Existing greyscale monogenic filterbanks are reviewed and we reveal a lack of discussion about the synthesis part. The monogenic synthesis is therefore defined from the idea of wavelet modulation, and an innovative filterbank is constructed by using the Radon transform. The color extension is then investigated. First, the elliptical Fourier atom model is proposed to generalize theanalytic signal representation for vector-valued signals. Then a color Riesz-transform is defined so as to construct color elliptical monogenic wavelets. Our Radon-based monogenic filterbank can be easily extended to color according to this definition. The proposed wavelet representation provides efficient analysis of local features in terms of shape and color, thanks to the concepts of amplitude, phase, orientation, and ellipse parameters. The synthesis from local features is deeply studied. We conclude the article by defining the color local frequency, proposing an estimation algorithm

Quaternionic Wavelets for Image Coding

5 pagesInternational audienceThe Quaternionic Wavelet Transform is a recent improvement of standard wavelets that has promising theoretical properties. This new transform has proved its superiority over standard wavelets in texture analysis, so we propose here to apply it in a wavelet based image coding process. The main point is the interpretation and coding of the QWT phase, which is not dealt with in the literature. At equal bitrates, our algorithm performs better visual quality than standard wavelet based method