Unmanned Aerial Vehicles (UAVs) in environmental biology: A Review

Acquiring information about the environment is a key step during each study in the field of environmental biology at different levels, from an individual species to community and biome. However, obtaining information about the environment is frequently difficult because of, for example, the phenological timing, spatial distribution of a species or limited accessibility of a particular area for the field survey. Moreover, remote sensing technology, which enables the observation of the Earth’s surface and is currently very common in environmental research, has many limitations such as insufficient spatial, spectral and temporal resolution and a high cost of data acquisition. Since the 1990s, researchers have been exploring the potential of different types of unmanned aerial vehicles (UAVs) for monitoring Earth’s surface. The present study reviews recent scientific literature dealing with the use of UAV in environmental biology. Amongst numerous papers, short communications and conference abstracts, we selected 110 original studies of how UAVs can be used in environmental biology and which organisms can be studied in this manner. Most of these studies concerned the use of UAV to measure the vegetation parameters such as crown height, volume, number of individuals (14 studies) and quantification of the spatio-temporal dynamics of vegetation changes (12 studies). UAVs were also frequently applied to count birds and mammals, especially those living in the water. Generally, the analytical part of the present study was divided into following sections: (1) detecting, assessing and predicting threats on vegetation, (2) measuring the biophysical parameters of vegetation, (3) quantifying the dynamics of changes in plants and habitats and (4) population and behaviour studies of animals. At the end, we also synthesised all the information showing, amongst others, the advances in environmental biology because of UAV application. Considering that 33% of studies found and included in this review were published in 2017 and 2018, it is expected that the number and variety of applications of UAVs in environmental biology will increase in the future

High-Throughput System for the Early Quantification of Major Architectural Traits in Olive Breeding Trials Using UAV Images and OBIA Techniques

The need for the olive farm modernization have encouraged the research of more efficient crop management strategies through cross-breeding programs to release new olive cultivars more suitable for mechanization and use in intensive orchards, with high quality production and resistance to biotic and abiotic stresses. The advancement of breeding programs are hampered by the lack of efficient phenotyping methods to quickly and accurately acquire crop traits such as morphological attributes (tree vigor and vegetative growth habits), which are key to identify desirable genotypes as early as possible. In this context, an UAV-based high-throughput system for olive breeding program applications was developed to extract tree traits in large-scale phenotyping studies under field conditions. The system consisted of UAV-flight configurations, in terms of flight altitude and image overlaps, and a novel, automatic, and accurate object-based image analysis (OBIA) algorithm based on point clouds, which was evaluated in two experimental trials in the framework of a table olive breeding program, with the aim to determine the earliest date for suitable quantifying of tree architectural traits. Two training systems (intensive and hedgerow) were evaluated at two very early stages of tree growth: 15 and 27 months after planting. Digital Terrain Models (DTMs) were automatically and accurately generated by the algorithm as well as every olive tree identified, independently of the training system and tree age. The architectural traits, specially tree height and crown area, were estimated with high accuracy in the second flight campaign, i.e. 27 months after planting. Differences in the quality of 3D crown reconstruction were found for the growth patterns derived from each training system. These key phenotyping traits could be used in several olive breeding programs, as well as to address some agronomical goals. In addition, this system is cost and time optimized, so that requested architectural traits could be provided in the same day as UAV flights. This high-throughput system may solve the actual bottleneck of plant phenotyping of "linking genotype and phenotype," considered a major challenge for crop research in the 21st century, and bring forward the crucial time of decision making for breeders

Using automated vegetation cover estimation from close-range photogrammetric point clouds to compare vegetation location properties in mountain terrain

In this paper we present a low-cost approach to mapping vegetation cover by means of high-resolution close-range terrestrial photogrammetry. A total of 249 clusters of nine 1 m2 plots each, arranged in a 3 × 3 grid, were set up on 18 summits in Mediterranean mountain regions and in the Alps to capture images for photogrammetric processing and in-situ vegetation cover estimates. This was done with a hand-held pole-mounted digital single-lens reflex (DSLR) camera. Low-growing vegetation was automatically segmented using high-resolution point clouds. For classifying vegetation we used a two-step semi-supervised Random Forest approach. First, we applied an expert-based rule set using the Excess Green index (ExG) to predefine non-vegetation and vegetation points. Second, we applied a Random Forest classifier to further enhance the classification of vegetation points using selected topographic parameters (elevation, slope, aspect, roughness, potential solar irradiation) and additional vegetation indices (Excess Green Minus Excess Red (ExGR) and the vegetation index VEG). For ground cover estimation the photogrammetric point clouds were meshed using Screened Poisson Reconstruction. The relative influence of the topographic parameters on the vegetation cover was determined with linear mixed-effects models (LMMs). Analysis of the LMMs revealed a high impact of elevation, aspect, solar irradiation, and standard deviation of slope. The presented approach goes beyond vegetation cover values based on conventional orthoimages and in-situ vegetation cover estimates from field surveys in that it is able to differentiate complete 3D surface areas, including overhangs, and can distinguish between vegetation-covered and other surfaces in an automated manner. The results of the Random Forest classification confirmed it as suitable for vegetation classification, but the relative feature importance values indicate that the classifier did not leverage the potential of the included topographic parameters. In contrast, our application of LMMs utilized the topographic parameters and was able to reveal dependencies in the two biomes, such as elevation and aspect, which were able to explain between 87% and 92.5% of variance