A microgripper for single cell manipulation

This thesis presents the development of an electrothermally actuated microgripper for the manipulation of cells and other biological particles. The microgripper has been fabricated using a combination of surface and bulk micromachining techniques in a three mask process. All of the fabrication details have been chosen to enable a tri-layer, polymer (SU8) - metal (Au) - polymer (SU8), membrane to be released from the substrate stress free and without the need for sacrificial layers. An actuator design, which completely eliminates the parasitic resistance of the cold arm, is presented. When compared to standard U-shaped actuators, it improves the thermal efficiency threefold. This enables larger displacements at lower voltages and temperatures. The microgripper is demonstrated in three different configurations: normally open mode, normally closed mode, and normally open/closed mode. It has-been modelled using two coupled analytical models - electrothermal and thermomechanical - which have been custom developed for this application. Unlike previously reported models, the electrothermal model presented here includes the heat exchange between hot and cold arms of the actuators that are separated by a small air gap. A detailed electrothermomechanical characterisation of selected devices has permitted the validation of the models (also performed using finite element analysis) and the assessment of device performance. The device testing includes electrical, deflection, and temperature measurements using infrared (IR) thermography, its use in polymeric actuators reported here for the first time. Successful manipulation experiments have been conducted in both air and liquid environments. Manipulation of live cells (mice oocytes) in a standard biomanipulation station has validated the microgripper as a complementary and unique tool for the single cell experiments that are to be conducted by future generations of biologists in the areas of human reproduction and stem cell research

Transport Characteristics of Pin Fin Enhanced Microgaps under Single and Two Phase Cooling

Microfluidic convection cooling is a promising technique for future high power microprocessors, radio-frequency (RF) transceivers, solid-state lasers, and light emitting diodes (LED). Three-dimensional (3D) stacking of chips is a configuration that allows many performance benefits. A microgap with circulating fluid is a promising cooling arrangement that can be incorporated within a 3D chip stack. Although studies have examined the thermal characteristics of microgaps under both single-phase and two-phase convection, the characteristics and benefits of microgaps with surface enhancement features have not been fully explored. In this work, firstly, the single phase thermal/fluid characteristics of microgaps with staggered pin fin arrays are studied. The effects of the pin fin dimensions including diameter, transversal and longitudinal spacing, and height are investigated computationally and experimentally over a range of Reynolds number (Re) 22-357. Micropin fin arrays investigated have pin diameter of 100 μm, pitch/ diameter ratios of 1.5 ~ 2.25, and height/ diameter ratios of 1.5 ~ 2.25. Correlations of friction factor (f) and Colburn j factor for these dense arrays of micro pins have been developed. Subsequently, microfluidic cooling with staggered pin fin arrays is employed in functional 3D integrated circuit (ICs). Thermal and electrical performance of a CMOS chip in terms of temperature and leakage power under realistic operating conditions are studied. Both experimental and modeling results show that microfluidic cooling could significantly decrease the chip temperature and leakage power, thus increasing the chip performance. Lastly, two-phase cooling is studied with dielectric fluid HFE-7200 as a baseline with mass flux from 354.5 kg/m2-s to 576.3 kg/m2-s. Critical heat flux (CHF) increases with increasing mass flux but decreases with decreasing gap height. Nonuniform heating will cause nonuniform flow with a decrease of mass flux in high power area, which decreases the thermal performance. The effects of fluid mixture (HFE-7200/Methanol) on thermal performance are studied with mass fraction of Methanol from 8.5% to 35.8%. A very small amount of addition of Methanol (8.5% mass fraction) can significantly increase the thermal performance due to the sharp decrease of saturation temperature and increase of effective thermal conductivity and latent heat. However, the Marangoni effect caused by the concentration gradient deteriorates the CHF.Ph.D

Hot-Wire Anemometer Measurements of Atmospheric Surface Layer Turbulence via Unmanned Aerial Vehicle

An instrumented unmanned aerial vehicle (UAV) was developed and employed to observe the full range of turbulent motions that exist within the inertial subrange of atmospheric surface layer turbulence. The UAV was host to a suite of pressure, temperature, humidity, and wind sensors which provide the necessary data to calculate the variety of turbulent statistics that characterize the flow. Flight experiments were performed with this aircraft, consisting of a large square pattern at an altitude of 100 m above ground level. In order to capture the largest turbulent scales it was necessary to maximize the size of the square pattern. The smallest turbulent scales, on the other hand, were measured through the use of a fast response constant temperature hot wire anemometer. The results demonstrates that the UAV system is capable of directly measuring the full inertial subrange of the atmospheric surface layer with high resolution and allowing for the turbulence dissipation rate to be calculated directly

In-Vitro and In-Silico Investigations of Alternative Surgical Techniques for Single Ventricular Disease

Single ventricle (SV) anomalies account for one-fourth of all cases of congenital Heart disease. The conventional second and third stage i.e. Comprehensive stage II and Fontan procedure of the existing three-staged surgical approach serving as a palliative treatment for this anomaly, entails multiple complications and achieves a survival rate of 50%. Hence, to reduce the morbidity and mortality rate associated with the second and third stages of the existing palliative procedure, the novel alternative techniques called “Hybrid Comprehensive Stage II” (HCSII), and a “Self-powered Fontan circulation” have been proposed. The goal of this research is to conduct in-vitro investigations to validate computational and clinical findings on these proposed novel surgical techniques. The research involves the development of a benchtop study of HCSII and self-powered Fontan circulation

Towards rapid 3D direct manufacture of biomechanical microstructures

The field of stereolithography has developed rapidly over the last 20 years, and commercially available systems currently have sufficient resolution for use in microengineering applications. However, they have not as yet been fully exploited in this field. This thesis investigates the possible microengineering applications of microstereolithography systems, specifically in the areas of active microfluidic devices and microneedles. The fields of micropumps and microvalves, stereolithography and microneedles are reviewed, and a variety of test builds were fabricated using the EnvisionTEC Perfactory Mini Multi-Lens stereolithography system in order to define its capabilities. A number of microneedle geometries were considered. This number was narrowed down using finite element modelling, before another simulation was used to optimise these structures. 9 × 9 arrays of 400 μm tall, 300 μm base diameter microneedles were subjected to mechanical testing. Per needle failure forces of 0.263 and 0.243 N were recorded for the selected geometries, stepped cone and inverted trumpet. The 90 μm needle tips were subjected to between 30 and 32 MPa of pressure at their failure point - more than 10 times the required pressure to puncture average human skin. A range of monolithic micropumps were produced with integrated 4 mm diameter single-layer 70 μm-thick membranes used as the basis for a reciprocating displacement operating principle. The membranes were tested using an oscillating pneumatic actuation, and were found reliable (>1,000,000 cycles) up to 2.0 PSIG. Pneumatic single-membrane nozzle/diffuser rectified devices produced flow rates of up to 1,000 μl/min with backpressures of up to 375 Pa. Another device rectified using active membrane valves was found to self-prime, and produced backpressures of up to 4.9 kPa. These devices and structures show great promise for inclusion in complex, fully integrated and active microfluidic systems fabricated using microstereolithography alone, with implications for both cost of manufacture and lead time

Design of microfluidic multiplex cartridge for point of care diagnostics

This thesis was submitted for the award of Doctor of Philosophy and was awarded by Brunel University LondonA simple, but innovative microfluidic Lab-on-a-chip (LOC) device which is broadly applicable in point of care diagnostics of biological pathogens was designed, fabricated and assembled utilising explicit microfluidic techniques. The purpose of this design was to develop a cartridge with the capability to perform multiplex DNA amplification reactions on a single device. To achieve this outcome, conventional laboratory protocols for sample preparation; involving DNA extraction, purification and elution were miniaturized to suit this lab-on-a-chip device of 75mm X 50mm cross-sectional area. The extraction process was carried out in a uniquely designed microchamber embedded with chitosan membrane that binds DNA at pH 5.0 and elutes when a different solution at pH 9.0 flows through. Likewise, purification protocol that occurs in the designed waste reservoir is very significant in biomedical field because it is concerned with waste treatment and cartridge disposability, was performed with a super absorbent powder that converts liquid to a gel like substance. This powder is known as sodium polyacrylate, which is also they treated with anti-bacterial chemicals to prevent environmental contamination. Furthermore, this process also employed the use of a passive valve for a precise fluid handling operation involving flow regulation from extraction to waste reservoir. In order to achieve the intended multiplexing function a multiplexer was created to distribute flow simultaneously through a bifurcated network of channels connected to six similar amplification microchambers. Prior to fabrication, computational fluid dynamics (CFD) simulation was utilized at flowrates less than 10μL/s as the means to test the effectiveness of each design components and also to specifically deduct empirical values that can be analyzed to improve or understand the relationship between the fluid and geometrical constraints of the microfluidic modular elements. The device produced was a hybrid cartridge composed of PDMS and glass which is the most widely used materials microfluidics research due to their low cost and simplicity of fabrication by soft lithography technique. The choice of material also took into account the various physical and chemical properties advantages and disadvantages in their bio-medical applications. Such properties include but not limited to surface energy that determines the wetting fluid characteristics, biocompatibility, optical transparency. Subsequently, after a prototype cartridge was developed fluid flow experimentation using liquid coloured dye was used on the fully fabricated cartridge to test the efficacy of its microfluidic functionalities before expensive DNA amplification reagents were utilised at similar flowrates to the CFD simulations. This gave rise to comparison between similar and dissimilar flow Peculiarities in the microfluidic circuit of both experiments. The final experiment was performed with the aid of a recent molecular technique in DNA amplification known as of RPA kit (recombinase polymerase amplification reaction). It involved performing two main reaction experiments; first, was the positive experiment that bears the sample DNA and the latter, negative that served as the control without DNA. In the end, quantitative analysis of results was done using an agarose gel that showed 143 base pairs, for the positive samples, thus validating the amplification experiment

Towards rapid 3D direct manufacture of biomechanical microstructures

The field of stereolithography has developed rapidly over the last 20 years, and commercially available systems currently have sufficient resolution for use in microengineering applications. However, they have not as yet been fully exploited in this field. This thesis investigates the possible microengineering applications of microstereolithography systems, specifically in the areas of active microfluidic devices and microneedles. The fields of micropumps and microvalves, stereolithography and microneedles are reviewed, and a variety of test builds were fabricated using the EnvisionTEC Perfactory Mini Multi-Lens stereolithography system in order to define its capabilities. A number of microneedle geometries were considered. This number was narrowed down using finite element modelling, before another simulation was used to optimise these structures. 9 × 9 arrays of 400 μm tall, 300 μm base diameter microneedles were subjected to mechanical testing. Per needle failure forces of 0.263 and 0.243 N were recorded for the selected geometries, stepped cone and inverted trumpet. The 90 μm needle tips were subjected to between 30 and 32 MPa of pressure at their failure point - more than 10 times the required pressure to puncture average human skin. A range of monolithic micropumps were produced with integrated 4 mm diameter single-layer 70 μm-thick membranes used as the basis for a reciprocating displacement operating principle. The membranes were tested using an oscillating pneumatic actuation, and were found reliable (>1,000,000 cycles) up to 2.0 PSIG. Pneumatic single-membrane nozzle/diffuser rectified devices produced flow rates of up to 1,000 μl/min with backpressures of up to 375 Pa. Another device rectified using active membrane valves was found to self-prime, and produced backpressures of up to 4.9 kPa. These devices and structures show great promise for inclusion in complex, fully integrated and active microfluidic systems fabricated using microstereolithography alone, with implications for both cost of manufacture and lead time.EThOS - Electronic Theses Online ServiceEngineering and Physical Sciences Research Council (EPSRC)GBUnited Kingdo

Microthermal Devices for Fluidic Actuation by Modulation of Surface Tension.

Fluid manipulation at the micrometer scale has traditionally involved the use of batch-fabricated chips containing miniature channels, electrodes, pumps, and other integrated structures. This dissertation explores how liquids on non-patterned substrates can be manipulated using the Marangoni effect. By placing miniature heat sources above a liquid film, it is possible to generate micro-scale surface temperature gradients which results in controlled Marangoni flow. A variety of useful flow patterns can be designed by tailoring the geometry of the heat source. As a surface tension-based phenomenon, the Marangoni effect is an efficient actuation mechanism at submillimeter dimensions. With optimized liquid carriers, flow velocities >10 mm/s can be generated with only small perturbations in surface temperature (1700 µm/s flow velocity in mineral oil while consuming <20 mW of power. In water films, the probes can generate surface doublets with linear velocities up to 5 mm/sec and rotational velocities up to 1300 rpm, making them potentially useful for active mixing. The utility of Marangoni flows is demonstrated within the context of digital microfluidic systems. In contrast to conventional microfluidics, where samples are flowed through microchannels, digital microfluidic systems contain liquid samples in micro and nanoliter-sized droplets suspended in an immiscible oil layer. Marangoni flows generated in the oil layer can manipulate droplets without any physical structures, thus avoiding surface contamination. By using point, linear, annular, and tapered heat source geometries, it is possible to engineer Marangoni flows which mimic the functionality of droplet channels, mixers, size-selective filters, and pumps. Arbitrary, two-dimensional actuation of droplets (Ф=400-1000 µm) can also be achieved using an array of heaters suspended above the oil layer. The 128-pixel heater array incorporates addressing logic and a software interface which allows it to programmatically transport and merge multiple droplets through the sequential activation of heaters. The appendices outline other aspects of thermal probes, including i) the structure, fabrication, and operational characteristics of single probes and probe arrays, and ii) scanning thermal lithography, a technique for nanoscale patterning of thin films with heat.Ph.D.Electrical EngineeringUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/60871/1/basua_1.pd

Experimental studies on the resistance to single and multiphase flow in a capillary tube

The resistance to the flow in pore space is of great significance because it controls how the fluids transmit the porous media over time. The fundamental of enhanced oil recovery, for example, is the displacement of two phases that occurred in the porous media, the resistance to the flow sets an obstacle for the displacement. Although many have presented their study on this subject, few considered the pore-scale study. This work focuses on the interface behavior study at pore level, presents an innovative way to measure the resistance to flows in a capillary, interprets and defines the effective pore throat and its application, defines the impact of the pore wetting, the effect of fluid property as well as the thermocapillarity on the resistance. The work presented in this thesis provides some fundamental understanding of two-phase fluids in a capillary network and will benefit research in enhanced oil recovery, fuel cell, and microfluidics, etc. To study the resistance to fluids, the pore wetting is an effective tool to start with. In this work, we creatively designed a method to visually observe and digitally analyze the contact angle variation during the imbibition. The dynamic pore contact angle at pore level is dependent mainly on the imbibition rate and fluid specifications, such as the surface tension and the viscosity. A regression equation describing the dynamic contact angle in the capillary is proposed based on the derivation of the empirical equation. The capillary resistances to the single-phase flow and the interface (the contact line between two immiscible phases) are measured and presented. The experimental data demonstrates a significant difference between the resistance to the single-phase and the interface. The measured resistance is compared with Washburn’s equation and Brooks-Corey’s model. Conclusively the resistance to the interface should be considered to understand the multiphase flow in the porous media. An innovative concept of ‘effective pore throat’ is proposed in this project based on our experiment result. The effective pore throat is the critical point where the resistance to the interface rises. It is affected by pore and fluid properties and provides basic principles for applications such as capillary hysteresis or clinical science. Thermocapillary is studied by laser-induced equipment. The temperature differential varies the surface tension along with the interface, creating unbalanced capillary pressure. In our findings, there is resistance to the system when the difference between the capillary pressure rises. The interface movement is tracked when the pressure difference is high enough. Furthermore, thermocapillary emulsification is demonstrated