8,968 research outputs found
Shape representation and coding of visual objets in multimedia applications — An overview
Emerging multimedia applications have created the need for new functionalities in digital communications. Whereas existing compression standards only deal with the audio-visual scene at a frame level, it is now necessary to handle individual objects separately, thus allowing scalable transmission as well as interactive scene recomposition by the receiver. The future MPEG-4 standard aims at providing compression tools addressing these functionalities. Unlike existing frame-based standards, the corresponding coding schemes need to encode shape information explicitly. This paper reviews existing solutions to the problem of shape representation and coding. Region and contour coding techniques are presented and their performance is discussed, considering coding efficiency and rate-distortion control capability, as well as flexibility to application requirements such as progressive transmission, low-delay coding, and error robustnes
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Parallel data compression
Data compression schemes remove data redundancy in communicated and stored data and increase the effective capacities of communication and storage devices. Parallel algorithms and implementations for textual data compression are surveyed. Related concepts from parallel computation and information theory are briefly discussed. Static and dynamic methods for codeword construction and transmission on various models of parallel computation are described. Included are parallel methods which boost system speed by coding data concurrently, and approaches which employ multiple compression techniques to improve compression ratios. Theoretical and empirical comparisons are reported and areas for future research are suggested
Performance Prediction of Nonbinary Forward Error Correction in Optical Transmission Experiments
In this paper, we compare different metrics to predict the error rate of
optical systems based on nonbinary forward error correction (FEC). It is shown
that the correct metric to predict the performance of coded modulation based on
nonbinary FEC is the mutual information. The accuracy of the prediction is
verified in a detailed example with multiple constellation formats, FEC
overheads in both simulations and optical transmission experiments over a
recirculating loop. It is shown that the employed FEC codes must be universal
if performance prediction based on thresholds is used. A tutorial introduction
into the computation of the threshold from optical transmission measurements is
also given.Comment: submitted to IEEE/OSA Journal of Lightwave Technolog
Control of DNA minor groove width and Fis protein binding by the purine 2-amino group.
The width of the DNA minor groove varies with sequence and can be a major determinant of DNA shape recognition by proteins. For example, the minor groove within the center of the Fis-DNA complex narrows to about half the mean minor groove width of canonical B-form DNA to fit onto the protein surface. G/C base pairs within this segment, which is not contacted by the Fis protein, reduce binding affinities up to 2000-fold over A/T-rich sequences. We show here through multiple X-ray structures and binding properties of Fis-DNA complexes containing base analogs that the 2-amino group on guanine is the primary molecular determinant controlling minor groove widths. Molecular dynamics simulations of free-DNA targets with canonical and modified bases further demonstrate that sequence-dependent narrowing of minor groove widths is modulated almost entirely by the presence of purine 2-amino groups. We also provide evidence that protein-mediated phosphate neutralization facilitates minor groove compression and is particularly important for binding to non-optimally shaped DNA duplexes
Processing and Transmission of Information
Contains reports on seven research projects.Lincoln Laboratory, Purchase Order DDL B-00337U.S. ArmyU.S. NavyU.S. Air Force under Air Force Contract AF19(604)-7400National Institutes of Health (Grant MH-04737-02
DNA Sequence Determinants Controlling Affinity, Stability and Shape of DNA Complexes Bound by the Nucleoid Protein Fis.
The abundant Fis nucleoid protein selectively binds poorly related DNA sequences with high affinities to regulate diverse DNA reactions. Fis binds DNA primarily through DNA backbone contacts and selects target sites by reading conformational properties of DNA sequences, most prominently intrinsic minor groove widths. High-affinity binding requires Fis-stabilized DNA conformational changes that vary depending on DNA sequence. In order to better understand the molecular basis for high affinity site recognition, we analyzed the effects of DNA sequence within and flanking the core Fis binding site on binding affinity and DNA structure. X-ray crystal structures of Fis-DNA complexes containing variable sequences in the noncontacted center of the binding site or variations within the major groove interfaces show that the DNA can adapt to the Fis dimer surface asymmetrically. We show that the presence and position of pyrimidine-purine base steps within the major groove interfaces affect both local DNA bending and minor groove compression to modulate affinities and lifetimes of Fis-DNA complexes. Sequences flanking the core binding site also modulate complex affinities, lifetimes, and the degree of local and global Fis-induced DNA bending. In particular, a G immediately upstream of the 15 bp core sequence inhibits binding and bending, and A-tracts within the flanking base pairs increase both complex lifetimes and global DNA curvatures. Taken together, our observations support a revised DNA motif specifying high-affinity Fis binding and highlight the range of conformations that Fis-bound DNA can adopt. The affinities and DNA conformations of individual Fis-DNA complexes are likely to be tailored to their context-specific biological functions
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