Ophthalmic sensing technologies for ocular disease diagnostics

Point-of-care diagnosis and personalized treatments are critical in ocular physiology and disease. Continuous sampling of tear fluid for ocular diagnosis is a need for further exploration. Several techniques have been developed for possible ophthalmological applications, from traditional spectroscopies to wearable sensors. Contact lenses are commonly used devices for vision correction, as well as for other therapeutic and cosmetic purposes. They are increasingly being developed into ocular sensors, being used to sense and monitor biochemical analytes in tear fluid, ocular surface temperature, intraocular pressure, and pH value. These sensors have had success in detecting ocular conditions, optimizing pharmaceutical treatments, and tracking treatment efficacy in point-of-care settings. However, there is a paucity of new and effective instrumentation reported in ophthalmology. Hence, this review will summarize the applied ophthalmic technologies for ocular diagnostics and tear monitoring, including both conventional and biosensing technologies. Besides applications of smart readout devices for continuous monitoring, targeted biomarkers are also discussed for the convenience of diagnosis of various ocular diseases. A further discussion is also provided for future aspects and market requirements related to the commercialization of novel types of contact lens sensors

Artwork imaging from 370 nm to 1630 nm using a novel multispectral system based on LEDs

The recent use of multispectral systems as a noncontact method for analysis of artworks has already shown promising results. This study explains the application of a novel portable multispectral system based on light-emitting diodes (LEDs) for artwork imaging. This method provides spectral information in a spectral range from 370 to 1630 nm with a 25 cm × 25 cm field of view by using two different image sensors in synchrony with 23 bands of irradiation. The spectral information for each point is estimated and validated using the pseudo-inverse and spline interpolation methods for spectral estimation and three different evaluation metrics. The results of the metrics obtained with both estimation methods show a general good performance of the system over the whole spectral range. The experiments also showed that the selection of the training set for the pseudo-inverse estimation has a great influence in its performance, and thus, it defines whether or not the pseudo-inverse outperforms the spline interpolation method. The system is applied in situ to the study of Catalan art masterpieces, and the results demonstrate the potential of a cost-effective and versatile system using various off-the-shelf elements to reconstruct color information and to reveal features not previously identified. © 2014 Wiley Periodicals, Inc. Col Res Appl, 201

Design and Construction of a Multispectral Camera for Spectral and Colorimetric Reproduction

Multi-spectral imaging and spectral reflectance reconstruction can be used in cultural-heritage institutes to digitalize their collections for documentation purposes. It can be used to simulate artwork under any lighting condition, and to analyze colorants that were used. The basic idea of a multi-spectral imaging system is to sub-sample spectral reflectance factor, producing results similar to a spectrophotometer. The sampled data are used to reconstruct reflectance for the visible spectrum. In this thesis, a wide band multispectral camera was designed and constructed to achieve high spectral and color accuracy as well as high image quality. Noise propagation theory was introduced and tested. A seven channel band- pass filter set was modeled using Gaussian functions and optimized to yield high spectral and colorimetric reproduction accuracy as well as low colori- metric noise. Single and sandwich filters were selected from o!-the-shelf absorption filters using the Gaussian bandpass filter model. Experiments were conducted to test the spectral, color and noise performance of the novel sandwich filters and compared with interference filters. The novel sandwich fil- ters led to increased colorimetric accuracy along with a reduction colorimetric noise. This imaging system will be used as part of a recommended workflow for museum archiving, and will be an important addition to the spectral imaging capabilities at MCSL

Digital Techniques for Documenting and Preserving Cultural Heritage

In this unique collection the authors present a wide range of interdisciplinary methods to study, document, and conserve material cultural heritage. The methods used serve as exemplars of best practice with a wide variety of cultural heritage objects having been recorded, examined, and visualised. The objects range in date, scale, materials, and state of preservation and so pose different research questions and challenges for digitization, conservation, and ontological representation of knowledge. Heritage science and specialist digital technologies are presented in a way approachable to non-scientists, while a separate technical section provides details of methods and techniques, alongside examples of notable applications of spatial and spectral documentation of material cultural heritage, with selected literature and identification of future research. This book is an outcome of interdisciplinary research and debates conducted by the participants of the COST Action TD1201, Colour and Space in Cultural Heritage, 2012–16 and is an Open Access publication available under a CC BY-NC-ND licence.https://scholarworks.wmich.edu/mip_arc_cdh/1000/thumbnail.jp

LED Selection for Spectral (Multispectral) Imaging

Research was performed to design an LED-based spectral imaging system having channels, commonly referred to as a multispectral imaging system. The first part tackled the evaluation of a camera model in predicting the signals of a 10 LED LEDmotive Technologies Spectra Tunelab coupled with a Finger Lakes Instrumentation panchromatic camera. The camera model was shown to be valid and effective in predicting the camera signal taking into account the color transformation noise. The second part involved the computational selection of 10 LEDs in order to determine the optimum combination for a custom Spectra Tunelab. The computational selection used the spectral data provided by the manufacturer for their 37 available LEDs. The LEDs were grouped according to a specified wavelength range. The binning process helped in decreasing the computational cost and time; the possible combinations were reduced to 110,592 from the initial calculated value of 348,330,136 possible combinations. The combinations were further reduced to 1000 according to spectral reflectance Root-Mean-Square-Error (RMSE). The Euclidean and score ranking methods were then used to evaluate color transformation noise, spectral error and colorimetric accuracy. Goodness of Fit Coefficient and Throughput were calculated as well to further evaluate the combinations. A compromise among the values were reached to identify the best possible LED combination. The optimal combination has peak wavelengths at 390 nm, 450 nm, 475 nm, 505 nm, 540 nm, 550 nm, 590 nm, 620 nm, 660 nm, and 745 nm. All the LEDs were narrow band except the LED with its peak wavelength at 550 nm. This particular LED was similar to the human visual system’s luminous efficiency function. Its inclusion was important for colorimetric accuracy and small color transformation noise. When evaluating a large color-gamut target made using commonly used commercial pigments and several artist pigments, the following quality metrics were achieved: average ∆E00 of 0.12, total Noise, N of 3.35, a lightness noise (∆L) of 1.22, spectral reflectance RMSE of 6.4 x10-3, GFC of 0.97 and a total throughput of 646.85

The Effects of Multi-channel Visible Spectrum Imaging on Perceived Spatial Image Quality and Color Reproduction Accuracy

Two paired-comparison psychophysical experiments were performed. The stimuli consisted of six image types resultingfrom several multispectral image-capture and reconstruction techniques. A seventh image type, color-managed images from a high-end consumer camera, was also included in thefirst experiment to compare the accuracy of commercial RGB imaging. The images were evaluated under simulated daylight (6800K) and incandescent (2700K) illumination. The first experiment evaluated color reproduction accuracy. Under simulated daylight, the subjects judged all of the images to have the same color accuracy, except the consumer camera image which was significantly worse. Under incandescent illumination, all the images, including the consumer camera, had equivalent performance. The second experiment evaluated image quality. The results of this experiment were highly target dependent. A subsequent image registration experiment showed that the results of the image quality experiment were affected by image registration to some degree. An analysis of the color reproduction accuracy and image quality experiments combined showed that the consumer camera image type was preferred the least over all. The most preferred image types were the thirty-one-channel image type and both six-channel image types created using RGB filters along with a Wratten filter, with eigenvector analysis and pseudo-inverse transformations

Digital Techniques for Documenting and Preserving Cultural Heritage

This book presents interdisciplinary approaches to the examination and documentation of material cultural heritage, using non-invasive spatial and spectral optical technologies

Ophthalmic sensing technologies for ocular disease diagnostics

Point-of-care diagnosis and personalized treatments are critical in ocular physiology and disease. Continuous sampling of tear fluid for ocular diagnosis is a need for further exploration. Several techniques have been developed for possible ophthalmological applications, from traditional spectroscopies to wearable sensors. Contact lenses are commonly used devices for vision correction, as well as for other therapeutic and cosmetic purposes. They are increasingly being developed into ocular sensors, being used to sense and monitor biochemical analytes in tear fluid, ocular surface temperature, intraocular pressure, and pH value. These sensors have had success in detecting ocular conditions, optimizing pharmaceutical treatments, and tracking treatment efficacy in point-of-care settings. However, there is a paucity of new and effective instrumentation reported in ophthalmology. Hence, this review will summarize the applied ophthalmic technologies for ocular diagnostics and tear monitoring, including both conventional and biosensing technologies. Besides applications of smart readout devices for continuous monitoring, targeted biomarkers are also discussed for the convenience of diagnosis of various ocular diseases. A further discussion is also provided for future aspects and market requirements related to the commercialization of novel types of contact lens sensors

Expanding Dimensionality in Cinema Color: Impacting Observer Metamerism through Multiprimary Display

Television and cinema display are both trending towards greater ranges and saturation of reproduced colors made possible by near-monochromatic RGB illumination technologies. Through current broadcast and digital cinema standards work, system designs employing laser light sources, narrow-band LED, quantum dots and others are being actively endorsed in promotion of Wide Color Gamut (WCG). Despite artistic benefits brought to creative content producers, spectrally selective excitations of naturally different human color response functions exacerbate variability of observer experience. An exaggerated variation in color-sensing is explicitly counter to the exhaustive controls and calibrations employed in modern motion picture pipelines. Further, singular standard observer summaries of human color vision such as found in the CIE’s 1931 and 1964 color matching functions and used extensively in motion picture color management are deficient in recognizing expected human vision variability. Many researchers have confirmed the magnitude of observer metamerism in color matching in both uniform colors and imagery but few have shown explicit color management with an aim of minimized difference in observer perception variability. This research shows that not only can observer metamerism influences be quantitatively predicted and confirmed psychophysically but that intentionally engineered multiprimary displays employing more than three primaries can offer increased color gamut with drastically improved consistency of experience. To this end, a seven-channel prototype display has been constructed based on observer metamerism models and color difference indices derived from the latest color vision demographic research. This display has been further proven in forced-choice paired comparison tests to deliver superior color matching to reference stimuli versus both contemporary standard RGB cinema projection and recently ratified standard laser projection across a large population of color-normal observers

Image segmentation and pigment mapping of cultural heritage based on spectral imaging

The goal of the work reported in this dissertation is to develop methods for image segmentation and pigment mapping of paintings based on spectral imaging. To reach this goal it is necessary to achieve sufficient spectral and colorimetric accuracies of both the spectral imaging system and pigment mapping. The output is a series of spatial distributions of pigments (or pigment maps) composing a painting. With these pigment maps, the change of the color appearance of the painting can be simulated when the optical properties of one or more pigments are altered. These pigment maps will also be beneficial for enriching the historical knowledge of the painting and aiding conservators in determining the best course for retouching damaged areas of the painting when metamerism is a factor. First, a new spectral reconstruction algorithm was developed based on Wyszecki’s hypothesis and the matrix R theory developed by Cohen and Kappauf. The method achieved both high spectral and colorimetric accuracies for a certain combination of illuminant and observer. The method was successfully tested with a practical spectral imaging system that included a traditional color-filter-array camera coupled with two optimized filters, developed in the Munsell Color Science Laboratory. The spectral imaging system was used to image test paintings, and the method was used to retrieve spectral reflectance factors for these paintings. Next, pigment mapping methods were brought forth, and these methods were based on Kubelka-Munk (K-M) turbid media theory that can predict spectral reflectance factor for a specimen from the optical properties of the specimen’s constituent pigments. The K-M theory has achieved practical success for opaque materials by reduction in mathematical complexity and elimination of controlling thickness. The use of the general K-M theory for the translucent samples was extensively studied, including determination of optical properties of pigments as functions of film thickness, and prediction of spectral reflectance factor of a specimen by selecting the right pigment combination. After that, an investigation was carried out to evaluate the impact of opacity and layer configuration of a specimen on pigment mapping. The conclusions were drawn from the comparisons of prediction accuracies of pigment mapping between opaque and translucent assumption, and between single and bi-layer assumptions. Finally, spectral imaging and pigment mapping were applied to three paintings. Large images were first partitioned into several small images, and each small image was segmented into different clusters based on either an unsupervised or supervised classification method. For each cluster, pigment mapping was done pixel-wise with a limited number of pigments, or with a limited number of pixels and then extended to other pixels based on a similarity calculation. For the masterpiece The Starry Night, these pigment maps can provide historical knowledge about the painting, aid conservators for inpainting damaged areas, and digitally rejuvenate the original color appearance of the painting (e.g. when the lead white was not noticeably darkened)