443 research outputs found

    SPATIAL ANALYSES AND REMOTE SENSING FOR LAND COVER CHANGE DYNAMICS: ASSESSING IN A SPATIAL PLANNING

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    ABSTRACT (EN) Spatial planning is a crucial discipline for the identification and implementation of sustainable development strategies that take into account the environmental impacts on the soil. In recent years, the significant development of technology, like remote sensing and GIS software, has significantly increased the understanding of environmental components, highlighting their peculiarities and criticalities. Geographically referenced information on environmental and socio-economic components represents a fundamental database for identifying and monitoring vulnerable areas, also distinguishing different levels of vulnerability. This is even more relevant considering the increasingly significant impact of land transformation processes, consisting of rapid and frequent changes in land use patterns. In order to achieve some of the Sustainable Development Goals of the 2030 Agenda, the role of environmental planning is crucial in addressing spatial problems, such as agricultural land abandonment and land take, which cause negative impacts on ecosystems. Remote sensing, and in general all Earth Observation techniques, play a key role in achieving SDG 11.3 and 15.3 of Agenda 2030. Through a series of applications and investigations in different areas of Basilicata, it has been demonstrated how the extensive use of remote sensing and spatial analysis in a GIS environment provide a substantial contribution to the results of the SDGs, enabling an informed decisionmaking process and enabling monitoring of the results expected, ensuring data reliability and directly contributing to the calculation of SDG objectives and indicators by facilitating local administrations approaches to work in different development and sustainability sectors. In this thesis have been analyse the dynamics of land transformation in terms of land take and soil erosion in sample areas of the Basilicata Region, which represents an interesting case example for the study of land use land cover change (LULCC). The socio-demographic evolutionary trends and the study of marginality and territorial fragility are fundamental aspects in the context of territorial planning, since they are important drivers of the LULCC and territorial transformation processes. In fact, in Basilicata, settlement dynamics over the years have occurred in an uncontrolled and unregulated manner, leading to a constant consumption of land not accompanied by adequate demographic and economic growth. To better understand the evolution and dynamics of the LULCCs and provide useful tools for formulating territorial planning policies and strategies aimed at a sustainable use of the territory, the socio-economic aspects of the Region were investigated. A first phase involved the creation of a database and the study and identification of essential services in the area as a fundamental parameter against which to evaluate the quality of life in a specific area. The supply of essential services can be understood as an assessment of the lack of minimum requirements with reference to the urban functions exercised by each territorial unit. From a territorial point of view, the level of peripherality of the territories with respect to the network of urban centres profoundly influences the quality of life of citizens and the level of social inclusion. In these, the presence of essential services can act as an attractor capable of generating discrete catchment areas. The purpose of this first part of the work was above all to create a dataset of data useful for the calculation of various socio-economic indicators, in order to frame the demographic evolution and the evolution of the stock of public and private services. The first methodological approach was to reconstruct the offer of essential services through the use of open data in a GIS environment and subsequently estimate the peripherality of each municipality by estimating the accessibility to essential services. The study envisaged the use of territorial analysis techniques aimed at describing the distribution of essential services on the regional territory. It is essential to understand the role of demographic dynamics as a driver of urban land use change such as, for example, the increase in demand for artificial surfaces that occurs locally. Social and economic analyses are important in the spatial planning process. Comparison of socio-economic analyses with land use and land cover change can highlight the need to modify existing policies or implement new ones. A particular land use can degrade and thereby destroy other land resources. If the economic analysis shows that the use is beneficial from the point of view of the land user, it is likely to continue, regardless of whether the process is environmentally friendly. It is important to understand and investigate which drivers have been and will be in the future the most decisive in these dynamics that intrinsically contribute to land take, agricultural abandonment and the consequent processes of land degradation and to define policies or thresholds to mitigate and monitor the effects of these processes. Subsequently, the issues of land take and abandonment of agricultural land were analysed by applying models and techniques of remote sensing, GIS and territorial analysis for the identification and monitoring of abandoned agricultural areas and sealed areas. The classic remote sensing methods have also been integrated by some geostatistical analyses which have provided more information on the investigated phenomenon. The aim was the creation of a quick methodology that would allow to describe the monitoring and analysis activities of the development trends of soil consumption and the monitoring and identification of degraded areas. The first methodology proposed allowed the automatic and rapid detection of detailed LULCC and Land Take maps with an overall accuracy of more than 90%, reducing costs and processing times. The identification of abandoned agricultural areas in degradation is among the most complicated LULCC and Land Degradation processes to identify and monitor as it is driven by a multiplicity of anthropic and natural factors. The model used to estimate soil erosion as a degradation phenomenon is the Revised Universal Soil Loss Equation (RUSLE). To identify potentially degraded areas, two factors of the RUSLE have been correlated: Factor C which describes the vegetation cover of the soil and Factor A which represents the amount of potential soil erosion. Through statistical correlation analysis with the RUSLE factors, on the basis of the deviations from the average RUSLE values and mapping of the areas of vegetation degradation, relating to arable land, through statistical correlation with the vegetation factor C, the areas were identified and mapped that are susceptible to soil degradation. The results obtained allowed the creation of a database and a map of the degraded areas to be paid attention to

    Mathematical Problems in Rock Mechanics and Rock Engineering

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    With increasing requirements for energy, resources and space, rock engineering projects are being constructed more often and are operated in large-scale environments with complex geology. Meanwhile, rock failures and rock instabilities occur more frequently, and severely threaten the safety and stability of rock engineering projects. It is well-recognized that rock has multi-scale structures and involves multi-scale fracture processes. Meanwhile, rocks are commonly subjected simultaneously to complex static stress and strong dynamic disturbance, providing a hotbed for the occurrence of rock failures. In addition, there are many multi-physics coupling processes in a rock mass. It is still difficult to understand these rock mechanics and characterize rock behavior during complex stress conditions, multi-physics processes, and multi-scale changes. Therefore, our understanding of rock mechanics and the prevention and control of failure and instability in rock engineering needs to be furthered. The primary aim of this Special Issue “Mathematical Problems in Rock Mechanics and Rock Engineering” is to bring together original research discussing innovative efforts regarding in situ observations, laboratory experiments and theoretical, numerical, and big-data-based methods to overcome the mathematical problems related to rock mechanics and rock engineering. It includes 12 manuscripts that illustrate the valuable efforts for addressing mathematical problems in rock mechanics and rock engineering

    PROBABILISTIC MODELING OF CHROMATIN INTERACTIONS

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    Higher-order chromatin architecture plays an important role in mammalian transcriptional regulation. However, understanding the mechanisms and impact of complex chromatin contacts remain challenging. In the past decade, breakthroughs in experimental techniques like Chromatin Conformation Capture (3C) assays enable genome-wide detection of chromatin interactions at high resolution. This provides great opportunities of computational modeling to predict functional interactions and identify target genes of disease variants. In this thesis, I started with critical assessment of an existing method for predicting enhancer-promoter interactions. I reported severe overfitting issues of it resulting from improper machine learning experimental design. I also found the limitation of resolution in their training datasets hinder accurate assignment of single regulatory element at interaction boundaries. In the second part, I developed a novel mathematical model to predict CTCF-mediated chromatin loops, which is the most prominent class of chromatin interactions, based on the biological hypothesis of loop extrusion. I showed that this model is capable of predicting CTCF loops measured by CTCF ChIA-PET data with high accuracy, using CTCF ChIP-seq alone as input. Furthermore, this model consistently predicts chromatin interaction frequency due to changes of CTCF binding site by genetic perturbation and looping-related protein factor degradation events. In the last part, I applied this computational framework to a greater set of ChIA-PET data. The analysis result inspired the development of a simple and interpretable for predicting enhancer-promoter interactions. I showed that this model outperforms existing methods on predicting CRISPRi hits that regulated gene expression. Overall, these approaches are applicable to diverse datasets to advance our understanding of chromatin interaction mechanisms as well as their implication in gene regulation and diseases

    A robotic platform for precision agriculture and applications

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    Agricultural techniques have been improved over the centuries to match with the growing demand of an increase in global population. Farming applications are facing new challenges to satisfy global needs and the recent technology advancements in terms of robotic platforms can be exploited. As the orchard management is one of the most challenging applications because of its tree structure and the required interaction with the environment, it was targeted also by the University of Bologna research group to provide a customized solution addressing new concept for agricultural vehicles. The result of this research has blossomed into a new lightweight tracked vehicle capable of performing autonomous navigation both in the open-filed scenario and while travelling inside orchards for what has been called in-row navigation. The mechanical design concept, together with customized software implementation has been detailed to highlight the strengths of the platform and some further improvements envisioned to improve the overall performances. Static stability testing has proved that the vehicle can withstand steep slopes scenarios. Some improvements have also been investigated to refine the estimation of the slippage that occurs during turning maneuvers and that is typical of skid-steering tracked vehicles. The software architecture has been implemented using the Robot Operating System (ROS) framework, so to exploit community available packages related to common and basic functions, such as sensor interfaces, while allowing dedicated custom implementation of the navigation algorithm developed. Real-world testing inside the university’s experimental orchards have proven the robustness and stability of the solution with more than 800 hours of fieldwork. The vehicle has also enabled a wide range of autonomous tasks such as spraying, mowing, and on-the-field data collection capabilities. The latter can be exploited to automatically estimate relevant orchard properties such as fruit counting and sizing, canopy properties estimation, and autonomous fruit harvesting with post-harvesting estimations.Le tecniche agricole sono state migliorate nel corso dei secoli per soddisfare la crescente domanda di aumento della popolazione mondiale. I recenti progressi tecnologici in termini di piattaforme robotiche possono essere sfruttati in questo contesto. Poiché la gestione del frutteto è una delle applicazioni più impegnative, a causa della sua struttura arborea e della necessaria interazione con l'ambiente, è stata oggetto di ricerca per fornire una soluzione personalizzata che sviluppi un nuovo concetto di veicolo agricolo. Il risultato si è concretizzato in un veicolo cingolato leggero, capace di effettuare una navigazione autonoma sia nello scenario di pieno campo che all'interno dei frutteti (navigazione interfilare). La progettazione meccanica, insieme all'implementazione del software, sono stati dettagliati per evidenziarne i punti di forza, accanto ad alcuni ulteriori miglioramenti previsti per incrementarne le prestazioni complessive. I test di stabilità statica hanno dimostrato che il veicolo può resistere a ripidi pendii. Sono stati inoltre studiati miglioramenti per affinare la stima dello slittamento che si verifica durante le manovre di svolta, tipico dei veicoli cingolati. L'architettura software è stata implementata utilizzando il framework Robot Operating System (ROS), in modo da sfruttare i pacchetti disponibili relativi a componenti base, come le interfacce dei sensori, e consentendo al contempo un'implementazione personalizzata degli algoritmi di navigazione sviluppati. I test in condizioni reali all'interno dei frutteti sperimentali dell'università hanno dimostrato la robustezza e la stabilità della soluzione con oltre 800 ore di lavoro sul campo. Il veicolo ha permesso di attivare e svolgere un'ampia gamma di attività agricole in maniera autonoma, come l'irrorazione, la falciatura e la raccolta di dati sul campo. Questi ultimi possono essere sfruttati per stimare automaticamente le proprietà più rilevanti del frutteto, come il conteggio e la calibratura dei frutti, la stima delle proprietà della chioma e la raccolta autonoma dei frutti con stime post-raccolta

    Chemometric tools for automated method-development and data interpretation in liquid chromatography

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    The thesis explores the challenges and advancements in the field of liquid chromatography (LC), particularly focusing on complex sample analysis using high-resolution mass spectrometry (MS) and two-dimensional (2D) LC techniques. The research addresses the need for efficient optimization and data-handling strategies in modern LC practice. The thesis is divided into several chapters, each addressing specific aspects of LC and polymer analysis. Chapter 2 provides an overview of the need for chemometric tools in LC practice, discussing methods for processing and analyzing data from 1D and 2D-LC systems and how chemometrics can be utilized for method development and optimization. Chapter 3 introduces a novel approach for interpreting the molecular-weight distribution and intrinsic viscosity of polymers, allowing quantitative analysis of polymer properties without prior knowledge of their interactions. This method correlates the curvature parameter of the Mark-Houwink plot with the polymer's structural and chemical properties. Chapters 4 and 5 focus on the analysis of cellulose ethers (CEs), essential in various industrial applications. A new method is presented for mapping the substitution degree and composition of CE samples, providing detailed compositional distributions. Another method involves a comprehensive 2D LC-MS/MS approach for analyzing hydroxypropyl methyl cellulose (HPMC) monomers, revealing subtle differences in composition between industrial HPMC samples. Chapter 6 introduces AutoLC, an algorithm for automated and interpretive development of 1D-LC separations. It uses retention modeling and Bayesian optimization to achieve optimal separation within a few iterations, significantly improving the efficiency of gradient LC separations. Chapter 7 focuses on the development of an open-source algorithm for automated method development in 2D-LC-MS systems. This algorithm improves separation performance by refining gradient profiles and accurately predicting peak widths, enhancing the reliability of complex gradient LC separations. Chapter 8 addresses the challenge of gradient deformation in LC instruments. An algorithm based on the stable function corrects instrument-specific gradient deformations, enabling accurate determination of analyte retention parameters and improving data comparability between different sources. Chapter 9 introduces a novel approach using capacitively-coupled-contactless-conductivity detection (C4D) to measure gradient profiles without adding tracer components. This method enhances inter-system transferability of retention models for polymers, overcoming the limitations of UV-absorbance detectable tracer components. Chapter 10 discusses practical choices and challenges faced in the thesis chapters, highlighting the need for well-defined standard samples in industrial polymer analysis and emphasizing the importance of generalized problem-solving approaches. The thesis identifies future research directions, emphasizing the importance of computational-assisted methods for polymer analysis, the utilization of online reaction modulation techniques, and exploring continuous distributions obtained through size-exclusion chromatography (SEC) in conjunction with triple detection. Chemometric tools are recognized as essential for gaining deeper insights into polymer chemistry and improving data interpretation in the field of LC
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