COLOR MULTIPLEXED SINGLE PATTERN SLI

Structured light pattern projection techniques are well known methods of accurately capturing 3-Dimensional information of the target surface. Traditional structured light methods require several different patterns to recover the depth, without ambiguity or albedo sensitivity, and are corrupted by object movement during the projection/capture process. This thesis work presents and discusses a color multiplexed structured light technique for recovering object shape from a single image thus being insensitive to object motion. This method uses single pattern whose RGB channels are each encoded with a unique subpattern. The pattern is projected on to the target and the reflected image is captured using high resolution color digital camera. The image is then separated into individual color channels and analyzed for 3-D depth reconstruction through use of phase decoding and unwrapping algorithms thereby establishing the viability of the color multiplexed single pattern technique. Compared to traditional methods (like PMP, Laser Scan etc) only one image/one-shot measurement is required to obtain the 3-D depth information of the object, requires less expensive hardware and normalizes albedo sensitivity and surface color reflectance variations. A cosine manifold and a flat surface are measured with sufficient accuracy demonstrating the feasibility of a real-time system

Video-rate volumetric neuronal imaging using 3D targeted illumination

Fast volumetric microscopy is required to monitor large-scale neural ensembles with high spatio-temporal resolution. Widefield fluorescence microscopy can image large 2D fields of view at high resolution and speed while remaining simple and costeffective. A focal sweep add-on can further extend the capacity of widefield microscopy by enabling extended-depth-of-field (EDOF) imaging, but suffers from an inability to reject out-of-focus fluorescence background. Here, by using a digital micromirror device to target only in-focus sample features, we perform EDOF imaging with greatly enhanced contrast and signal-to-noise ratio, while reducing the light dosage delivered to the sample. Image quality is further improved by the application of a robust deconvolution algorithm. We demonstrate the advantages of our technique for in vivo calcium imaging in the mouse brain.This work was funded by the National Institutes of Health (R21EY026310) and the National Science Foundation (CBET-1508988). The authors wish to thank E. McCarthy and Prof. M.J. Baum for providing mouse brain slices used in this manuscript, and A. I. Mohammed for providing in vivo mouse brain samples in the early stages of this work. (R21EY026310 - National Institutes of Health; CBET-1508988 - National Science Foundation)Published versio