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KARR-seq reveals cellular higher-order RNA structures and RNA–RNA interactions
RNA fate and function are affected by their structures and interactomes. However, how RNA and RNA-binding proteins (RBPs) assemble into higher-order structures and how RNA molecules may interact with each other to facilitate functions remain largely unknown. Here we present KARR-seq, which uses N3-kethoxal labeling and multifunctional chemical crosslinkers to covalently trap and determine RNA–RNA interactions and higher-order RNA structures inside cells, independent of local protein binding to RNA. KARR-seq depicts higher-order RNA structure and detects widespread intermolecular RNA–RNA interactions with high sensitivity and accuracy. Using KARR-seq, we show that translation represses mRNA compaction under native and stress conditions. We determined the higher-order RNA structures of respiratory syncytial virus (RSV) and vesicular stomatitis virus (VSV) and identified RNA–RNA interactions between the viruses and the host RNAs that potentially regulate viral replication
A Survey on Forensics and Compliance Auditing for Critical Infrastructure Protection
The broadening dependency and reliance that modern societies have on essential services
provided by Critical Infrastructures is increasing the relevance of their trustworthiness. However, Critical
Infrastructures are attractive targets for cyberattacks, due to the potential for considerable impact, not just
at the economic level but also in terms of physical damage and even loss of human life. Complementing
traditional security mechanisms, forensics and compliance audit processes play an important role in ensuring
Critical Infrastructure trustworthiness. Compliance auditing contributes to checking if security measures are
in place and compliant with standards and internal policies. Forensics assist the investigation of past security
incidents. Since these two areas significantly overlap, in terms of data sources, tools and techniques, they can
be merged into unified Forensics and Compliance Auditing (FCA) frameworks. In this paper, we survey the
latest developments, methodologies, challenges, and solutions addressing forensics and compliance auditing
in the scope of Critical Infrastructure Protection. This survey focuses on relevant contributions, capable of
tackling the requirements imposed by massively distributed and complex Industrial Automation and Control
Systems, in terms of handling large volumes of heterogeneous data (that can be noisy, ambiguous, and
redundant) for analytic purposes, with adequate performance and reliability. The achieved results produced
a taxonomy in the field of FCA whose key categories denote the relevant topics in the literature. Also, the
collected knowledge resulted in the establishment of a reference FCA architecture, proposed as a generic
template for a converged platform. These results are intended to guide future research on forensics and
compliance auditing for Critical Infrastructure Protection.info:eu-repo/semantics/publishedVersio
ENHANCING CLOUD SYSTEM RUNTIME TO ADDRESS COMPLEX FAILURES
As the reliance on cloud systems intensifies in our progressively digital world, understanding and reinforcing their reliability becomes more crucial than ever. Despite impressive advancements in augmenting the resilience of cloud systems, the growing incidence of complex failures now poses a substantial challenge to the availability of these systems. With cloud systems continuing to scale and increase in complexity, failures not only become more elusive to detect but can also lead to more catastrophic consequences. Such failures question the foundational premises of conventional fault-tolerance designs, necessitating the creation of novel system designs to counteract them.
This dissertation aims to enhance distributed systems’ capabilities to detect, localize, and react to complex failures at runtime. To this end, this dissertation makes contributions to address three emerging categories of failures in cloud systems. The first part delves into the investigation of partial failures, introducing OmegaGen, a tool adept at generating tailored checkers for detecting and localizing such failures. The second part grapples with silent semantic failures prevalent in cloud systems, showcasing our study findings, and introducing Oathkeeper, a tool that leverages past failures to infer rules and expose these silent issues. The third part explores solutions to slow failures via RESIN, a framework specifically designed to detect, diagnose, and mitigate memory leaks in cloud-scale infrastructures, developed in collaboration with Microsoft Azure. The dissertation concludes by offering insights into future directions for the construction of reliable cloud systems
UMSL Bulletin 2023-2024
The 2023-2024 Bulletin and Course Catalog for the University of Missouri St. Louis.https://irl.umsl.edu/bulletin/1088/thumbnail.jp
Natural and Technological Hazards in Urban Areas
Natural hazard events and technological accidents are separate causes of environmental impacts. Natural hazards are physical phenomena active in geological times, whereas technological hazards result from actions or facilities created by humans. In our time, combined natural and man-made hazards have been induced. Overpopulation and urban development in areas prone to natural hazards increase the impact of natural disasters worldwide. Additionally, urban areas are frequently characterized by intense industrial activity and rapid, poorly planned growth that threatens the environment and degrades the quality of life. Therefore, proper urban planning is crucial to minimize fatalities and reduce the environmental and economic impacts that accompany both natural and technological hazardous events
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